Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 187935011

GI number: 187935011

Start: 2798274

End: 2799401

Strand: Reverse

Name: fadH [C]

Synonym: CLL_A2684

Alternate gene names: 187935011

Gene position: 2799401-2798274 (Counterclockwise)

Preceding gene: 187933502

Following gene: 187934711

Centisome position: 73.66

GC content: 29.7

Gene sequence:

>1128_bases
ATGAAAAGTCTTTGGGATAAAACAAATATAGGTAAAATGAAATTAAAAAATAGATTTTTTAGAGGTGCACTTTGGGAAGA
GTTAGCAGGTGAAAAAGGGCATTTAACACCTGAATTAAGTTCAATCTATGAAGAACTGGCAAAGGGTGGAGTAGGTACAA
TAATAACAGGTTATGCTTTTGTAACAAAAGATGAGCAACCTAATCCAAGAATGATGGGAATATATAATGATTCATTTATA
AAGGAATATAAAGAATTTACAAATAAAATACATGATTTAGGTGCAAATATAATAATGCAAATAGTTTATGGTGGATTTAT
GACTACTTTTAATGTAGGGGAAAGAACTATATGGGGTCCATCAACTATGCAGAATGAAGTTACTAAGACTTGGGCAAAAG
AAATGACTAAAGATGAAATAAAATATTTAGTTAATGCATATTCAGAAGCAGCAAGAAGAGTTAAGGAATCAGGTTTTGAT
GGAGTTGAGATACATGGTGGACATGGATATTTACTTAGTCAATTTTTATCACCATATTACAATAAAAGAACTGATGAATA
TGGGGGTAGCATAGAAAATAGAGGAAGAATAATATTTGAAATCTTTCAAGCTATGAGAGAAAAAGTAGGTAACGATTTCC
CAATTTTAATAAAACTTAATTCTTCTGACTATATTAAAGAAGGTGGATTAAATAAAGAGGAAAGCTTATATGTAGCTAAA
AAACTTGCTAACTTAGGAATAGATGCTATTGAGGTAACTGGTGGAAATGAATCAATACAAGAAGTATTAGATAATAATAT
GGGAGCAGCTCGTACTAAAGTAGTAATATCTAAGGAAAGAGAATCATACTTTAAAGATTATGCTACAGGGCTTGCAGCTA
ATATAGATATACCAGTTATTTTAATAGGTGGTAACAGACATTTAGATGTTATGGAAGATTTGTTAAATAATGAAAATATA
GAATATTTTACATTGTCAAGACCTCTAACTGCTGAACCAGATTTAATAAATAAATGGGCAACTGGCAATTTAAAGAATCC
TAAATGTGTATCATGTAATCAATGTTATAACACACCAGGTAAAAGATGTATTTTAAATATTAGAGAAGCAGAAAAGAAGA
ATAAGTAA

Upstream 100 bases:

>100_bases
ATAATATCAAGTTACTAAAAGGTGCATACTTGTTAGACTTTTAATATGGCGTTATAGTATTTATAAGCCGCGGATAACTT
AATTCAAAGGAGAGATTTTA

Downstream 100 bases:

>100_bases
GTTGCATGAATTATAAATTAAGTCTATTATACTATAGACTTTTAAGTTTTAATATCAGAAGAGAATTATTATTTACTCCT
ATAATGGGGGGAGAATCAAG

Product: oxidoreductase, FAD/FMN-binding

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKSLWDKTNIGKMKLKNRFFRGALWEELAGEKGHLTPELSSIYEELAKGGVGTIITGYAFVTKDEQPNPRMMGIYNDSFI
KEYKEFTNKIHDLGANIIMQIVYGGFMTTFNVGERTIWGPSTMQNEVTKTWAKEMTKDEIKYLVNAYSEAARRVKESGFD
GVEIHGGHGYLLSQFLSPYYNKRTDEYGGSIENRGRIIFEIFQAMREKVGNDFPILIKLNSSDYIKEGGLNKEESLYVAK
KLANLGIDAIEVTGGNESIQEVLDNNMGAARTKVVISKERESYFKDYATGLAANIDIPVILIGGNRHLDVMEDLLNNENI
EYFTLSRPLTAEPDLINKWATGNLKNPKCVSCNQCYNTPGKRCILNIREAEKKNK

Sequences:

>Translated_375_residues
MKSLWDKTNIGKMKLKNRFFRGALWEELAGEKGHLTPELSSIYEELAKGGVGTIITGYAFVTKDEQPNPRMMGIYNDSFI
KEYKEFTNKIHDLGANIIMQIVYGGFMTTFNVGERTIWGPSTMQNEVTKTWAKEMTKDEIKYLVNAYSEAARRVKESGFD
GVEIHGGHGYLLSQFLSPYYNKRTDEYGGSIENRGRIIFEIFQAMREKVGNDFPILIKLNSSDYIKEGGLNKEESLYVAK
KLANLGIDAIEVTGGNESIQEVLDNNMGAARTKVVISKERESYFKDYATGLAANIDIPVILIGGNRHLDVMEDLLNNENI
EYFTLSRPLTAEPDLINKWATGNLKNPKCVSCNQCYNTPGKRCILNIREAEKKNK
>Mature_375_residues
MKSLWDKTNIGKMKLKNRFFRGALWEELAGEKGHLTPELSSIYEELAKGGVGTIITGYAFVTKDEQPNPRMMGIYNDSFI
KEYKEFTNKIHDLGANIIMQIVYGGFMTTFNVGERTIWGPSTMQNEVTKTWAKEMTKDEIKYLVNAYSEAARRVKESGFD
GVEIHGGHGYLLSQFLSPYYNKRTDEYGGSIENRGRIIFEIFQAMREKVGNDFPILIKLNSSDYIKEGGLNKEESLYVAK
KLANLGIDAIEVTGGNESIQEVLDNNMGAARTKVVISKERESYFKDYATGLAANIDIPVILIGGNRHLDVMEDLLNNENI
EYFTLSRPLTAEPDLINKWATGNLKNPKCVSCNQCYNTPGKRCILNIREAEKKNK

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=386, Percent_Identity=26.9430051813472, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787939, Length=270, Percent_Identity=28.1481481481481, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=31.1953352769679, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17559802, Length=347, Percent_Identity=30.5475504322767, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17540738, Length=360, Percent_Identity=31.9444444444444, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17559804, Length=347, Percent_Identity=31.4121037463977, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI72001454, Length=355, Percent_Identity=30.9859154929577, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17564188, Length=349, Percent_Identity=30.6590257879656, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17566914, Length=378, Percent_Identity=30.4232804232804, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17566916, Length=317, Percent_Identity=27.1293375394322, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=236, Percent_Identity=31.7796610169492, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321973, Length=186, Percent_Identity=32.7956989247312, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 42385; Mature: 42385

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLWDKTNIGKMKLKNRFFRGALWEELAGEKGHLTPELSSIYEELAKGGVGTIITGYAF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECEEE
VTKDEQPNPRMMGIYNDSFIKEYKEFTNKIHDLGANIIMQIVYGGFMTTFNVGERTIWGP
EECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCC
STMQNEVTKTWAKEMTKDEIKYLVNAYSEAARRVKESGFDGVEIHGGHGYLLSQFLSPYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
NKRTDEYGGSIENRGRIIFEIFQAMREKVGNDFPILIKLNSSDYIKEGGLNKEESLYVAK
CCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHH
KLANLGIDAIEVTGGNESIQEVLDNNMGAARTKVVISKERESYFKDYATGLAANIDIPVI
HHHHCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHEECCCEEEE
LIGGNRHLDVMEDLLNNENIEYFTLSRPLTAEPDLINKWATGNLKNPKCVSCNQCYNTPG
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCEECCHHHHCCCC
KRCILNIREAEKKNK
HHEEEECHHHHCCCC
>Mature Secondary Structure
MKSLWDKTNIGKMKLKNRFFRGALWEELAGEKGHLTPELSSIYEELAKGGVGTIITGYAF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECEEE
VTKDEQPNPRMMGIYNDSFIKEYKEFTNKIHDLGANIIMQIVYGGFMTTFNVGERTIWGP
EECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCC
STMQNEVTKTWAKEMTKDEIKYLVNAYSEAARRVKESGFDGVEIHGGHGYLLSQFLSPYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
NKRTDEYGGSIENRGRIIFEIFQAMREKVGNDFPILIKLNSSDYIKEGGLNKEESLYVAK
CCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHH
KLANLGIDAIEVTGGNESIQEVLDNNMGAARTKVVISKERESYFKDYATGLAANIDIPVI
HHHHCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHEECCCEEEE
LIGGNRHLDVMEDLLNNENIEYFTLSRPLTAEPDLINKWATGNLKNPKCVSCNQCYNTPG
EECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCEECCHHHHCCCC
KRCILNIREAEKKNK
HHEEEECHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]