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The map label for this gene is ilvB [H]

Identifier: 187934417

GI number: 187934417

Start: 3434227

End: 3435858

Strand: Reverse

Name: ilvB [H]

Synonym: CLL_A3272

Alternate gene names: 187934417

Gene position: 3435858-3434227 (Counterclockwise)

Preceding gene: 187933201

Following gene: 187933671

Centisome position: 90.41

GC content: 28.25

Gene sequence:

>1632_bases
GTGAAATTATCTAAAGCTATAGCAAAATATATTGAAAGTTGTGGTGTGGAATACGTATTTGGTATACCATCAGGAAATGT
AGCAAGTATTTATGATGCACTTAATGACACTAAAGTAGAATTTGTAGTAACTAAAAATGAAGCAGGATCAACTTATTCAG
CAGGAAAATATTCAGATTTAAGTAAAAAACTTAGCTGTTCAATTTTATGTGGAGGGGTAGGTATTACTAATGCAATAAAT
GGAATAGCAGATGCATACATAAATAATATTCCTATGCTTGTTATTAGTGGATATGCAGCAAGTGATAGCATAGGCAAAGG
TTGTGTTCAAGAATTAGATACAGCACCTATAACTGAAAGCATAACAAAATATAGTAAAACAATTACTAAAAAAGAAGATG
TATTATCTGAATTAGATAAAGCAGTAAAATTAGCAGTGCAAGACCCAAAAGGACCAGTTCATTTATCAATATCTATGGAT
TTACCTACAACAGAAATAAATGAAGAGGATATAAAAACTTATGTAGCTGAAAAAATAGATTATAATTATGATCAAGAATC
ATTAAATATAGCAATCAATATGTTAAATGATGCTAAAAAAGGTATTATACTAGTTGGTAGAGGTGCTAAAGGAAATAGTG
AAATTATAAAGAATTTATCATCAAAATTAGGATGGTATGTAGCTACTACCCCTCAAGGAAAAAGTTCAATAGATAGTGAA
TTTGAATTTAATATAGGAAACTATGGATTTAACAGCACTGATTTCGCTCAAAACTATGCAGATACAGAAGAAATAGATTG
TGTATTAGTATTAGGATCAAGCTTAGGAGAAAGTGCAACAAGAAACTTTAATAATGCCTTATTTGATGATGGAAACAAAG
TTATTCAAATAGATAACAAGAAAAAGAGCTTAAATAAAAATCATATTGATAGATTTAAAGTATATTATGAATTATCAGAA
GCATTATCAATAATAAATGAAAAAGTTGAAGATAAAAATGTGACTTTAAATAAAGTAGAAAAGTTAAATAATGATTATGT
AAAAGATCATACAGGAATATCTGTTAGAAGATTAGCAGACATGCTTCCTAACATATTAAATCCTAGTACTACTTATATGT
GTGATATCGGTGAATTTATGAATTACATGTATAAATATCTGTTTATTCCAAAAGAATCAGATTTTATTGCTAGTTTAAAT
TATGGAGCTATGGGAAATTGTGTAGCAGGTTCAATAGGTGCAGCATTAAGTGGAAATACAGAAAAATATGCTGTGGTTGT
TGGAGATGGATCAGTATTTATGAATGGTAGTGAAATTTTAACAGCAAAACAATACTCATTACCTATAATATATTTTGTAA
TAAATAATGCAAAATTAAATTACGTTGACCAGGGAATGACATTCCTATTTGGAAGATCTATTAATGGAATAGTTCAAGAT
AGAGTTTCAATAAAGGCAATAGGAGAAGCAGCTGGAATTAAATCATATGAAATAACTGATTTAAGTCAGCTTAATGAAAT
AAAAGATGAGTTATATTCATGTAGTGAACCTATAATAGTTGAAATAATAACTGATGGTTCAGAAAAAGTTGCAGCAGCAG
ATAGATTCAAAACATTAAAGAATAAAAAGTAA

Upstream 100 bases:

>100_bases
TTTAAAGAAGAACATAAATATTCAGATGAAGAAAAATCAAAAGCAGTAAGCATAGTAAAAAAATATAATAAGATTTTATG
TATTGAGGAGATGTTTAAAC

Downstream 100 bases:

>100_bases
GAACATGTTTTAAGGAAAGTGTTGATATATGCATAAAAAAGTGGACTGAGTAATTGAACTTAGGAATACTAAAAATGAGT
ATAGTTCCATTTTAGCTTGT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKLSKAIAKYIESCGVEYVFGIPSGNVASIYDALNDTKVEFVVTKNEAGSTYSAGKYSDLSKKLSCSILCGGVGITNAIN
GIADAYINNIPMLVISGYAASDSIGKGCVQELDTAPITESITKYSKTITKKEDVLSELDKAVKLAVQDPKGPVHLSISMD
LPTTEINEEDIKTYVAEKIDYNYDQESLNIAINMLNDAKKGIILVGRGAKGNSEIIKNLSSKLGWYVATTPQGKSSIDSE
FEFNIGNYGFNSTDFAQNYADTEEIDCVLVLGSSLGESATRNFNNALFDDGNKVIQIDNKKKSLNKNHIDRFKVYYELSE
ALSIINEKVEDKNVTLNKVEKLNNDYVKDHTGISVRRLADMLPNILNPSTTYMCDIGEFMNYMYKYLFIPKESDFIASLN
YGAMGNCVAGSIGAALSGNTEKYAVVVGDGSVFMNGSEILTAKQYSLPIIYFVINNAKLNYVDQGMTFLFGRSINGIVQD
RVSIKAIGEAAGIKSYEITDLSQLNEIKDELYSCSEPIIVEIITDGSEKVAAADRFKTLKNKK

Sequences:

>Translated_543_residues
MKLSKAIAKYIESCGVEYVFGIPSGNVASIYDALNDTKVEFVVTKNEAGSTYSAGKYSDLSKKLSCSILCGGVGITNAIN
GIADAYINNIPMLVISGYAASDSIGKGCVQELDTAPITESITKYSKTITKKEDVLSELDKAVKLAVQDPKGPVHLSISMD
LPTTEINEEDIKTYVAEKIDYNYDQESLNIAINMLNDAKKGIILVGRGAKGNSEIIKNLSSKLGWYVATTPQGKSSIDSE
FEFNIGNYGFNSTDFAQNYADTEEIDCVLVLGSSLGESATRNFNNALFDDGNKVIQIDNKKKSLNKNHIDRFKVYYELSE
ALSIINEKVEDKNVTLNKVEKLNNDYVKDHTGISVRRLADMLPNILNPSTTYMCDIGEFMNYMYKYLFIPKESDFIASLN
YGAMGNCVAGSIGAALSGNTEKYAVVVGDGSVFMNGSEILTAKQYSLPIIYFVINNAKLNYVDQGMTFLFGRSINGIVQD
RVSIKAIGEAAGIKSYEITDLSQLNEIKDELYSCSEPIIVEIITDGSEKVAAADRFKTLKNKK
>Mature_543_residues
MKLSKAIAKYIESCGVEYVFGIPSGNVASIYDALNDTKVEFVVTKNEAGSTYSAGKYSDLSKKLSCSILCGGVGITNAIN
GIADAYINNIPMLVISGYAASDSIGKGCVQELDTAPITESITKYSKTITKKEDVLSELDKAVKLAVQDPKGPVHLSISMD
LPTTEINEEDIKTYVAEKIDYNYDQESLNIAINMLNDAKKGIILVGRGAKGNSEIIKNLSSKLGWYVATTPQGKSSIDSE
FEFNIGNYGFNSTDFAQNYADTEEIDCVLVLGSSLGESATRNFNNALFDDGNKVIQIDNKKKSLNKNHIDRFKVYYELSE
ALSIINEKVEDKNVTLNKVEKLNNDYVKDHTGISVRRLADMLPNILNPSTTYMCDIGEFMNYMYKYLFIPKESDFIASLN
YGAMGNCVAGSIGAALSGNTEKYAVVVGDGSVFMNGSEILTAKQYSLPIIYFVINNAKLNYVDQGMTFLFGRSINGIVQD
RVSIKAIGEAAGIKSYEITDLSQLNEIKDELYSCSEPIIVEIITDGSEKVAAADRFKTLKNKK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=490, Percent_Identity=22.2448979591837, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI21361361, Length=155, Percent_Identity=21.2903225806452, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790104, Length=532, Percent_Identity=22.1804511278195, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=26.5938069216758, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=22.5454545454545, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=18.9716312056738, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531299, Length=494, Percent_Identity=25.7085020242915, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=494, Percent_Identity=25.7085020242915, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17542570, Length=155, Percent_Identity=23.8709677419355, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=542, Percent_Identity=23.2472324723247, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320816, Length=322, Percent_Identity=23.6024844720497, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=488, Percent_Identity=21.9262295081967, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=478, Percent_Identity=20.9205020920502, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922626, Length=475, Percent_Identity=22.7368421052632, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59427; Mature: 59427

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKAIAKYIESCGVEYVFGIPSGNVASIYDALNDTKVEFVVTKNEAGSTYSAGKYSDL
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHH
SKKLSCSILCGGVGITNAINGIADAYINNIPMLVISGYAASDSIGKGCVQELDTAPITES
HHCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHH
ITKYSKTITKKEDVLSELDKAVKLAVQDPKGPVHLSISMDLPTTEINEEDIKTYVAEKID
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCC
YNYDQESLNIAINMLNDAKKGIILVGRGAKGNSEIIKNLSSKLGWYVATTPQGKSSIDSE
CCCCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCC
FEFNIGNYGFNSTDFAQNYADTEEIDCVLVLGSSLGESATRNFNNALFDDGNKVIQIDNK
EEEECCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEEEEECCC
KKSLNKNHIDRFKVYYELSEALSIINEKVEDKNVTLNKVEKLNNDYVKDHTGISVRRLAD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHH
MLPNILNPSTTYMCDIGEFMNYMYKYLFIPKESDFIASLNYGAMGNCVAGSIGAALSGNT
HHHHHCCCCCEEEECHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHCCCCCEECCCC
EKYAVVVGDGSVFMNGSEILTAKQYSLPIIYFVINNAKLNYVDQGMTFLFGRSINGIVQD
CEEEEEEECCEEEECCCCEEEEECCCCCEEEEEEECCEEEEHHCCCCEEECCCCCCHHHH
RVSIKAIGEAAGIKSYEITDLSQLNEIKDELYSCSEPIIVEIITDGSEKVAAADRFKTLK
CCCEEEHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHC
NKK
CCC
>Mature Secondary Structure
MKLSKAIAKYIESCGVEYVFGIPSGNVASIYDALNDTKVEFVVTKNEAGSTYSAGKYSDL
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHH
SKKLSCSILCGGVGITNAINGIADAYINNIPMLVISGYAASDSIGKGCVQELDTAPITES
HHCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHH
ITKYSKTITKKEDVLSELDKAVKLAVQDPKGPVHLSISMDLPTTEINEEDIKTYVAEKID
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCC
YNYDQESLNIAINMLNDAKKGIILVGRGAKGNSEIIKNLSSKLGWYVATTPQGKSSIDSE
CCCCCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCC
FEFNIGNYGFNSTDFAQNYADTEEIDCVLVLGSSLGESATRNFNNALFDDGNKVIQIDNK
EEEECCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEEEEECCC
KKSLNKNHIDRFKVYYELSEALSIINEKVEDKNVTLNKVEKLNNDYVKDHTGISVRRLAD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHH
MLPNILNPSTTYMCDIGEFMNYMYKYLFIPKESDFIASLNYGAMGNCVAGSIGAALSGNT
HHHHHCCCCCEEEECHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHCCCCCEECCCC
EKYAVVVGDGSVFMNGSEILTAKQYSLPIIYFVINNAKLNYVDQGMTFLFGRSINGIVQD
CEEEEEEECCEEEECCCCEEEEECCCCCEEEEEEECCEEEEHHCCCCEEECCCCCCHHHH
RVSIKAIGEAAGIKSYEITDLSQLNEIKDELYSCSEPIIVEIITDGSEKVAAADRFKTLK
CCCEEEHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHC
NKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]