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The map label for this gene is asnB [H]

Identifier: 187934146

GI number: 187934146

Start: 1435631

End: 1437220

Strand: Reverse

Name: asnB [H]

Synonym: CLL_A1395

Alternate gene names: 187934146

Gene position: 1437220-1435631 (Counterclockwise)

Preceding gene: 187932871

Following gene: 187935679

Centisome position: 37.82

GC content: 32.52

Gene sequence:

>1590_bases
ATGTGTACAATTATGTGTTATACAGGAACAGATATGAAACATGAAAAATTTGCTGAGGCATTACAAAGAACTGAGTCTAG
AGGACCAGATATGACTGAAATTCTAACTTTACCATCTGGTATTTTAGGTTTTCAAAGACTTGCTATTATGGATTTAAGTT
TTGGTGGAATGCAACCATTCACAAGAAAAAAAGATGCATCTATCTGTAATGGTGAAATTTATGGATTCCGTAAATTAAAA
GAAGAACTAATGGCAAAAGGCCATAAATTTATTTCTGACAGTGATTGCGAAATATTACTTCCAATGTACTTAGAGTACGG
ACTAGATATGTTCTCAAAATTAGATGCAGAATTCGCAGCTGTTATCTATGATGGAGAAAAAGATATTTTCATTGCAGCAA
GAGATCCAATTGGAATTCGACCTCTATTCTATGGCTATTCTGAATGTGGTAAAATCATGTTTGCTAGTGAAGCTAAAAAT
TTAGTTGGCCTTACAGAAAAGATAATGTCATTTCCACCAGGACATTATTATGCTGATGGTAAATTTACATGCTACTGTGA
TATCGCATCAGTAGAGGGTGATTACTGTAAAGATGACTTGGACGTTATTTGTAGTAACATTCATGACAAATTAGTAACTG
CTATAGAAAAACGTATGGATGCTGATGCTTCAGTAGGTTTCTTATTAAGTGGTGGCTTAGACAGCTCATTAGTATGTTCA
ATTGCTCAAAAAATGTATCCTAATAAACCAATAAAAACCTTTGCTATAGGTATGACAGAAGATGCCATTGACTTAAAATA
TGCAAAAGAAGTTGCAGATTACATTCATAGTGATCATACTGTTGTTTACATGACAAAAGAAGAAGTTCTTGACTCACTTG
AAGAAGTTATTAAACTTCTTGGAACATTTGATATTACAACAATAAGAGCTAGTATTGGTATGTATTTAATTTGTAAAAAA
ATACATGAGATTTCTGATGTTAGAGTACTTTTAACTGGTGAAATCTCTGATGAATTATTTGGATATAAATATACTGATTT
TGCGCCAAATGCAGAAGAATTCCAAAAAGAATCACAAAAGAGAATTAAAGAACTCTTTATGTATGATGTACTTCGAGCAG
ACCGTTGTATCGCATCTAATTCTTTAGAAGCAAGAGTGCCATTTGGCGATTTGGATTTTGTAAAATATGTTATGTCTATT
GATCCTGAAAAGAAATTAAACAAATACAATATGGGTAAATACCTTTTAAGACATTCTTTTGAAGGTGATTATCTTCCACA
TAATATTTTATATCGTGAAAAAGCAGCCTTTAGCGATGCTGTAGGACATTCTATGGTAGATTATTTAAAGGAGTATGCAG
AAACAGTCTATAATGGTGAAGATTTTGATAAAATAAGTGAAAAGTATAACTACCATGCTCAACCATTCACAAAAGAATCA
TTGTTATATAGAGAAATTTTTGAAAAATACTATCCAGGCCAAGCAGAAATGATAGTGGACTTTTGGATGCCAAACAAAGA
ATGGGAAGGCTGTGCTGTAGACGATCCTAGTGCCAGAGCATTATCAAATTATGGTGATAGTGGACTATAA

Upstream 100 bases:

>100_bases
ATGTTTATAGGATGAAGGGCTTTTTTTATTAGAAAAAAGTTTAATAGAGATAAATATTTGAATAATTACATTTTTTATTT
AATTGAGGAGGAATATTAAT

Downstream 100 bases:

>100_bases
TTAATAAACCAAATTTGTTTATTCTATCTGATTATTATTTAGCTTAATTATAGCAACCTCATCATGACACTATGATGAGG
TTACTTATATACTGTGTTAT

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MCTIMCYTGTDMKHEKFAEALQRTESRGPDMTEILTLPSGILGFQRLAIMDLSFGGMQPFTRKKDASICNGEIYGFRKLK
EELMAKGHKFISDSDCEILLPMYLEYGLDMFSKLDAEFAAVIYDGEKDIFIAARDPIGIRPLFYGYSECGKIMFASEAKN
LVGLTEKIMSFPPGHYYADGKFTCYCDIASVEGDYCKDDLDVICSNIHDKLVTAIEKRMDADASVGFLLSGGLDSSLVCS
IAQKMYPNKPIKTFAIGMTEDAIDLKYAKEVADYIHSDHTVVYMTKEEVLDSLEEVIKLLGTFDITTIRASIGMYLICKK
IHEISDVRVLLTGEISDELFGYKYTDFAPNAEEFQKESQKRIKELFMYDVLRADRCIASNSLEARVPFGDLDFVKYVMSI
DPEKKLNKYNMGKYLLRHSFEGDYLPHNILYREKAAFSDAVGHSMVDYLKEYAETVYNGEDFDKISEKYNYHAQPFTKES
LLYREIFEKYYPGQAEMIVDFWMPNKEWEGCAVDDPSARALSNYGDSGL

Sequences:

>Translated_529_residues
MCTIMCYTGTDMKHEKFAEALQRTESRGPDMTEILTLPSGILGFQRLAIMDLSFGGMQPFTRKKDASICNGEIYGFRKLK
EELMAKGHKFISDSDCEILLPMYLEYGLDMFSKLDAEFAAVIYDGEKDIFIAARDPIGIRPLFYGYSECGKIMFASEAKN
LVGLTEKIMSFPPGHYYADGKFTCYCDIASVEGDYCKDDLDVICSNIHDKLVTAIEKRMDADASVGFLLSGGLDSSLVCS
IAQKMYPNKPIKTFAIGMTEDAIDLKYAKEVADYIHSDHTVVYMTKEEVLDSLEEVIKLLGTFDITTIRASIGMYLICKK
IHEISDVRVLLTGEISDELFGYKYTDFAPNAEEFQKESQKRIKELFMYDVLRADRCIASNSLEARVPFGDLDFVKYVMSI
DPEKKLNKYNMGKYLLRHSFEGDYLPHNILYREKAAFSDAVGHSMVDYLKEYAETVYNGEDFDKISEKYNYHAQPFTKES
LLYREIFEKYYPGQAEMIVDFWMPNKEWEGCAVDDPSARALSNYGDSGL
>Mature_529_residues
MCTIMCYTGTDMKHEKFAEALQRTESRGPDMTEILTLPSGILGFQRLAIMDLSFGGMQPFTRKKDASICNGEIYGFRKLK
EELMAKGHKFISDSDCEILLPMYLEYGLDMFSKLDAEFAAVIYDGEKDIFIAARDPIGIRPLFYGYSECGKIMFASEAKN
LVGLTEKIMSFPPGHYYADGKFTCYCDIASVEGDYCKDDLDVICSNIHDKLVTAIEKRMDADASVGFLLSGGLDSSLVCS
IAQKMYPNKPIKTFAIGMTEDAIDLKYAKEVADYIHSDHTVVYMTKEEVLDSLEEVIKLLGTFDITTIRASIGMYLICKK
IHEISDVRVLLTGEISDELFGYKYTDFAPNAEEFQKESQKRIKELFMYDVLRADRCIASNSLEARVPFGDLDFVKYVMSI
DPEKKLNKYNMGKYLLRHSFEGDYLPHNILYREKAAFSDAVGHSMVDYLKEYAETVYNGEDFDKISEKYNYHAQPFTKES
LLYREIFEKYYPGQAEMIVDFWMPNKEWEGCAVDDPSARALSNYGDSGL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=571, Percent_Identity=37.1278458844133, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI168229252, Length=571, Percent_Identity=37.1278458844133, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI168229250, Length=571, Percent_Identity=37.1278458844133, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI296010848, Length=545, Percent_Identity=37.9816513761468, Blast_Score=295, Evalue=1e-79,
Organism=Homo sapiens, GI296010852, Length=471, Percent_Identity=39.0658174097665, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI296010850, Length=471, Percent_Identity=39.0658174097665, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1786889, Length=531, Percent_Identity=35.7815442561205, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17560178, Length=509, Percent_Identity=36.5422396856582, Blast_Score=293, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25147557, Length=541, Percent_Identity=35.3049907578558, Blast_Score=280, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71993933, Length=539, Percent_Identity=35.0649350649351, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI25147560, Length=277, Percent_Identity=34.6570397111913, Blast_Score=128, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=580, Percent_Identity=38.2758620689655, Blast_Score=300, Evalue=3e-82,
Organism=Saccharomyces cerevisiae, GI6321563, Length=578, Percent_Identity=37.7162629757785, Blast_Score=300, Evalue=4e-82,
Organism=Drosophila melanogaster, GI45553209, Length=519, Percent_Identity=35.8381502890173, Blast_Score=294, Evalue=8e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 60195; Mature: 60195

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTIMCYTGTDMKHEKFAEALQRTESRGPDMTEILTLPSGILGFQRLAIMDLSFGGMQPF
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHEECCCCCCCCC
TRKKDASICNGEIYGFRKLKEELMAKGHKFISDSDCEILLPMYLEYGLDMFSKLDAEFAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEE
VIYDGEKDIFIAARDPIGIRPLFYGYSECGKIMFASEAKNLVGLTEKIMSFPPGHYYADG
EEEECCCEEEEEECCCCCCCHHHCCHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCEEECC
KFTCYCDIASVEGDYCKDDLDVICSNIHDKLVTAIEKRMDADASVGFLLSGGLDSSLVCS
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
IAQKMYPNKPIKTFAIGMTEDAIDLKYAKEVADYIHSDHTVVYMTKEEVLDSLEEVIKLL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
GTFDITTIRASIGMYLICKKIHEISDVRVLLTGEISDELFGYKYTDFAPNAEEFQKESQK
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHH
RIKELFMYDVLRADRCIASNSLEARVPFGDLDFVKYVMSIDPEKKLNKYNMGKYLLRHSF
HHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCC
EGDYLPHNILYREKAAFSDAVGHSMVDYLKEYAETVYNGEDFDKISEKYNYHAQPFTKES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHH
LLYREIFEKYYPGQAEMIVDFWMPNKEWEGCAVDDPSARALSNYGDSGL
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MCTIMCYTGTDMKHEKFAEALQRTESRGPDMTEILTLPSGILGFQRLAIMDLSFGGMQPF
CEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHEECCCCCCCCC
TRKKDASICNGEIYGFRKLKEELMAKGHKFISDSDCEILLPMYLEYGLDMFSKLDAEFAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEE
VIYDGEKDIFIAARDPIGIRPLFYGYSECGKIMFASEAKNLVGLTEKIMSFPPGHYYADG
EEEECCCEEEEEECCCCCCCHHHCCHHHHCCEEEHHHHHHHHHHHHHHHHCCCCCEEECC
KFTCYCDIASVEGDYCKDDLDVICSNIHDKLVTAIEKRMDADASVGFLLSGGLDSSLVCS
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
IAQKMYPNKPIKTFAIGMTEDAIDLKYAKEVADYIHSDHTVVYMTKEEVLDSLEEVIKLL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
GTFDITTIRASIGMYLICKKIHEISDVRVLLTGEISDELFGYKYTDFAPNAEEFQKESQK
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHH
RIKELFMYDVLRADRCIASNSLEARVPFGDLDFVKYVMSIDPEKKLNKYNMGKYLLRHSF
HHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHCC
EGDYLPHNILYREKAAFSDAVGHSMVDYLKEYAETVYNGEDFDKISEKYNYHAQPFTKES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHH
LLYREIFEKYYPGQAEMIVDFWMPNKEWEGCAVDDPSARALSNYGDSGL
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]