Definition Clostridium botulinum B str. Eklund 17B, complete genome.
Accession NC_010674
Length 3,800,327

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The map label for this gene is polA [H]

Identifier: 187933407

GI number: 187933407

Start: 2759376

End: 2761991

Strand: Reverse

Name: polA [H]

Synonym: CLL_A2645

Alternate gene names: 187933407

Gene position: 2761991-2759376 (Counterclockwise)

Preceding gene: 187935310

Following gene: 187934279

Centisome position: 72.68

GC content: 26.34

Gene sequence:

>2616_bases
ATGAAAAGATTATTAATTTTAGATTCAAATTCGCTTATGAATAGGGCATTTTATGCTTTACCTCCTCTTACTAATGATGA
CGGTGTAAATACTAATGCTATTTATGGCTTTACAAATATGTTATTTAAAATGAAAGATGAAATAAATCCAGATAATATAA
TTGCAACATTTGATAAGAAGTCGCCAACATTTAGACATAAAGAATACTCAGAGTATAAAGCTGGAAGAAAGAAAATGCCA
CCAGAACTTGGTGAACAATTTCCAATTATAAAAGAATTACTTAAATATATGGGAATAAAGACTTTTGAAATAGATGGTTT
TGAAGCAGATGACATAATAGGAACTATATCAAAATTTGGTGAAGAAAATGATATGGAAGTTTATATAGTTACAGGAGATA
AGGATGCTCTTCAATTAGCATCAGAAAAAACAAAAGTAGTTATAACTAAGAAAGGTGTTACTGAAACAGCAATTTATGAT
TATAAAGCATTTATGGATGAATTTGAAATAACACCAACTCAATTTATAGACGTAAAAGGGCTTATGGGAGATAAATCTGA
TAATATACCAGGGGTACCAGGAGTTGGAGAGAAGACAGCATTTAAACTTATTAAAGAATATGGAACACTTGAAGATGTTT
TAAAGCATATACCAGAGATAAGTGGTAAGAAGTTAAAAGAAAACTTAGAAAATAATATGGAACAAGCAATATTTTCTAAG
AAATTAGCTACAATAATGAGGGAAGTACCATTAGACATTAATTTATCAGATATAGAAAATGCTGATGAAGTAAATTTAGT
AGAACTTAAAAAATTATCTAAAAATCTTAAATTAAAATCAGTACTATCAAAGTATGATTTAGATAATAATGAAGCAGAGT
TACAAGAGATAGCTAATATAGAAATAAGTAAAATAGAAACAATTGAAGAGATGGAAGAGGTATTATTAAGTATTAAAGAT
AGAAGTTATATTTTATTTACATTAAATGATGCATCTAAATACTCTACAATAGAATTAGAATACTTAATATTAGGCGAAGA
AGGCAGATCACATGTTATAGATTTTAAGCTAGTAAGTATGTCAAATAGAGAAAAAGCTTTAAATTTATTAAGAACCTTTA
TGGAAGATAAAACTGTTAAAAAAGTAATACATGATAGCAAAAATTTAATTACATTTTTAAACAAGTATGATATAGATATT
CAAGGTTTTGATTTTGATACAGCCATAGCAGCTTATTTAATAGACTCTTCAAAAGCCAATTATGAAGTTTTTGATTTAAT
TAATAGATTTTTAGAAAAAGATATAAAGTCTGATTGTGATGATCTAAAAGCAATATGCAGCAGTTATTTAACAAAATTAT
ATGAAAAGTTAAAAGAAAGGCTTCATAAAGAAGATATGGATGAATTGTATTATAAAGTAGAACATCCATTAATATATGTA
CTTTCTTCAATGGAAAGCATTGGATTCAAAATAAATAAAGATAAATTAGAAGAACTTAGAATAAAATTCAAAGAACAAAT
AGAAAAGACAGAAAAAGAAATCTATGAATTAGCAGACGAAGAATTCAATGTAAGCTCACCAAAGCAACTTGGAAAAATAC
TATTTGAAAAACTTGATTTGCCAGTAATTAAGAAAACTAAAACAGGGTATTCAACAAATCAAGAAGTTTTAGAAAAATTA
ATAGACAAACATCCTATAATAACTAAAGTTATGTATTATAGACAAATTACTAAAATTAATTCTACTTATGTAGAGGGACT
TAAGAATGTAATAGATATAGATGGATGTATACATTCAAATTTCAATCAAACTGTCACAACTACAGGAAGATTATCAAGTA
CTGAACCTAACCTTCAAAATATACCAATAAAATATGAAATGGGAAAAGAAATAAGAAAAGTGTTTATACCACAGGATAGT
GATGATATATTAATATCTTGCGATTATTCTCAAATAGAATTAAGAGTACTTGCACATGTAGCAGATGATAAAAATATGAT
AGATGCATTTGAACATCATAGTGATATACATACCAAAACAGCTTCAGAAGTTTTTAAAGTTCCTGTAGATGAAGTTACAT
CTACAATGAGAAGTAATGCAAAAGCAGTAAATTTTGGTATAGTTTATGGAATAAGTGACTTTAGTTTAGCACAAGATTTA
AAAATTACTAAAAAAGAAGCAGCAGAGTATATGGCTATTTATTTTGAAAGATATCCTAAGATAAAGGAATATTTAAATTA
TGTAAGTGAATACGCTAAAGAAAAAGGTTATGTATTAACTATATTAAACAGAAGAAGATTCATACCAGAAATAAAATCTT
CAAATAAAATTGTAAAAGCATTAGGTGAAAGATTAGCTATGAATGCGCCAATACAAGGAAGTGCAGCTGATATTATTAAA
CTTGCTATGGTTAATGTTTATAATAAGTTAAGAGAAAAGAATTTAAATAGTGAATTAATTCTTCAAGTACACGATGAACT
TATATTAAATGTTAAAAAAGATGAGTTTGATATAGTTAAACAATTGGTTATTGAAGAAATGGAAAAGGCTATTAAATTAA
ATGTAGCACTTGATGTAGATTTAAAATACGGTTATACATGGTATGATGCAAAATAA

Upstream 100 bases:

>100_bases
AGAATAATGATTTTACTTGATAAAGATAGTATAATATTAATGTATAACTAATTTTATATACAATAAAAGTATCTTAAAGA
TATTCAGGAGGATTATATCA

Downstream 100 bases:

>100_bases
AAATGGGAGATGAACTTTTTAAATGATAAAGATAGGGTTAACAGGGGGTATAGGTACTGGAAAAAGTACTATATCAGATA
TATTTAGAAGTGAAAACTTT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 871; Mature: 871

Protein sequence:

>871_residues
MKRLLILDSNSLMNRAFYALPPLTNDDGVNTNAIYGFTNMLFKMKDEINPDNIIATFDKKSPTFRHKEYSEYKAGRKKMP
PELGEQFPIIKELLKYMGIKTFEIDGFEADDIIGTISKFGEENDMEVYIVTGDKDALQLASEKTKVVITKKGVTETAIYD
YKAFMDEFEITPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGTLEDVLKHIPEISGKKLKENLENNMEQAIFSK
KLATIMREVPLDINLSDIENADEVNLVELKKLSKNLKLKSVLSKYDLDNNEAELQEIANIEISKIETIEEMEEVLLSIKD
RSYILFTLNDASKYSTIELEYLILGEEGRSHVIDFKLVSMSNREKALNLLRTFMEDKTVKKVIHDSKNLITFLNKYDIDI
QGFDFDTAIAAYLIDSSKANYEVFDLINRFLEKDIKSDCDDLKAICSSYLTKLYEKLKERLHKEDMDELYYKVEHPLIYV
LSSMESIGFKINKDKLEELRIKFKEQIEKTEKEIYELADEEFNVSSPKQLGKILFEKLDLPVIKKTKTGYSTNQEVLEKL
IDKHPIITKVMYYRQITKINSTYVEGLKNVIDIDGCIHSNFNQTVTTTGRLSSTEPNLQNIPIKYEMGKEIRKVFIPQDS
DDILISCDYSQIELRVLAHVADDKNMIDAFEHHSDIHTKTASEVFKVPVDEVTSTMRSNAKAVNFGIVYGISDFSLAQDL
KITKKEAAEYMAIYFERYPKIKEYLNYVSEYAKEKGYVLTILNRRRFIPEIKSSNKIVKALGERLAMNAPIQGSAADIIK
LAMVNVYNKLREKNLNSELILQVHDELILNVKKDEFDIVKQLVIEEMEKAIKLNVALDVDLKYGYTWYDAK

Sequences:

>Translated_871_residues
MKRLLILDSNSLMNRAFYALPPLTNDDGVNTNAIYGFTNMLFKMKDEINPDNIIATFDKKSPTFRHKEYSEYKAGRKKMP
PELGEQFPIIKELLKYMGIKTFEIDGFEADDIIGTISKFGEENDMEVYIVTGDKDALQLASEKTKVVITKKGVTETAIYD
YKAFMDEFEITPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGTLEDVLKHIPEISGKKLKENLENNMEQAIFSK
KLATIMREVPLDINLSDIENADEVNLVELKKLSKNLKLKSVLSKYDLDNNEAELQEIANIEISKIETIEEMEEVLLSIKD
RSYILFTLNDASKYSTIELEYLILGEEGRSHVIDFKLVSMSNREKALNLLRTFMEDKTVKKVIHDSKNLITFLNKYDIDI
QGFDFDTAIAAYLIDSSKANYEVFDLINRFLEKDIKSDCDDLKAICSSYLTKLYEKLKERLHKEDMDELYYKVEHPLIYV
LSSMESIGFKINKDKLEELRIKFKEQIEKTEKEIYELADEEFNVSSPKQLGKILFEKLDLPVIKKTKTGYSTNQEVLEKL
IDKHPIITKVMYYRQITKINSTYVEGLKNVIDIDGCIHSNFNQTVTTTGRLSSTEPNLQNIPIKYEMGKEIRKVFIPQDS
DDILISCDYSQIELRVLAHVADDKNMIDAFEHHSDIHTKTASEVFKVPVDEVTSTMRSNAKAVNFGIVYGISDFSLAQDL
KITKKEAAEYMAIYFERYPKIKEYLNYVSEYAKEKGYVLTILNRRRFIPEIKSSNKIVKALGERLAMNAPIQGSAADIIK
LAMVNVYNKLREKNLNSELILQVHDELILNVKKDEFDIVKQLVIEEMEKAIKLNVALDVDLKYGYTWYDAK
>Mature_871_residues
MKRLLILDSNSLMNRAFYALPPLTNDDGVNTNAIYGFTNMLFKMKDEINPDNIIATFDKKSPTFRHKEYSEYKAGRKKMP
PELGEQFPIIKELLKYMGIKTFEIDGFEADDIIGTISKFGEENDMEVYIVTGDKDALQLASEKTKVVITKKGVTETAIYD
YKAFMDEFEITPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGTLEDVLKHIPEISGKKLKENLENNMEQAIFSK
KLATIMREVPLDINLSDIENADEVNLVELKKLSKNLKLKSVLSKYDLDNNEAELQEIANIEISKIETIEEMEEVLLSIKD
RSYILFTLNDASKYSTIELEYLILGEEGRSHVIDFKLVSMSNREKALNLLRTFMEDKTVKKVIHDSKNLITFLNKYDIDI
QGFDFDTAIAAYLIDSSKANYEVFDLINRFLEKDIKSDCDDLKAICSSYLTKLYEKLKERLHKEDMDELYYKVEHPLIYV
LSSMESIGFKINKDKLEELRIKFKEQIEKTEKEIYELADEEFNVSSPKQLGKILFEKLDLPVIKKTKTGYSTNQEVLEKL
IDKHPIITKVMYYRQITKINSTYVEGLKNVIDIDGCIHSNFNQTVTTTGRLSSTEPNLQNIPIKYEMGKEIRKVFIPQDS
DDILISCDYSQIELRVLAHVADDKNMIDAFEHHSDIHTKTASEVFKVPVDEVTSTMRSNAKAVNFGIVYGISDFSLAQDL
KITKKEAAEYMAIYFERYPKIKEYLNYVSEYAKEKGYVLTILNRRRFIPEIKSSNKIVKALGERLAMNAPIQGSAADIIK
LAMVNVYNKLREKNLNSELILQVHDELILNVKKDEFDIVKQLVIEEMEKAIKLNVALDVDLKYGYTWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=508, Percent_Identity=32.6771653543307, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI139394648, Length=533, Percent_Identity=28.3302063789869, Blast_Score=215, Evalue=2e-55,
Organism=Escherichia coli, GI1790294, Length=540, Percent_Identity=40.5555555555556, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI226510970, Length=211, Percent_Identity=28.9099526066351, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI86563272, Length=378, Percent_Identity=30.4232804232804, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI17933644, Length=550, Percent_Identity=29.2727272727273, Blast_Score=226, Evalue=5e-59,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100201; Mature: 100201

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLILDSNSLMNRAFYALPPLTNDDGVNTNAIYGFTNMLFKMKDEINPDNIIATFDKK
CCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCC
SPTFRHKEYSEYKAGRKKMPPELGEQFPIIKELLKYMGIKTFEIDGFEADDIIGTISKFG
CCCCCCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
EENDMEVYIVTGDKDALQLASEKTKVVITKKGVTETAIYDYKAFMDEFEITPTQFIDVKG
CCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECC
LMGDKSDNIPGVPGVGEKTAFKLIKEYGTLEDVLKHIPEISGKKLKENLENNMEQAIFSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KLATIMREVPLDINLSDIENADEVNLVELKKLSKNLKLKSVLSKYDLDNNEAELQEIANI
HHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCC
EISKIETIEEMEEVLLSIKDRSYILFTLNDASKYSTIELEYLILGEEGRSHVIDFKLVSM
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEEEECC
SNREKALNLLRTFMEDKTVKKVIHDSKNLITFLNKYDIDIQGFDFDTAIAAYLIDSSKAN
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCC
YEVFDLINRFLEKDIKSDCDDLKAICSSYLTKLYEKLKERLHKEDMDELYYKVEHPLIYV
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
LSSMESIGFKINKDKLEELRIKFKEQIEKTEKEIYELADEEFNVSSPKQLGKILFEKLDL
HHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
PVIKKTKTGYSTNQEVLEKLIDKHPIITKVMYYRQITKINSTYVEGLKNVIDIDGCIHSN
CEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
FNQTVTTTGRLSSTEPNLQNIPIKYEMGKEIRKVFIPQDSDDILISCDYSQIELRVLAHV
CCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHH
ADDKNMIDAFEHHSDIHTKTASEVFKVPVDEVTSTMRSNAKAVNFGIVYGISDFSLAQDL
HCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHH
KITKKEAAEYMAIYFERYPKIKEYLNYVSEYAKEKGYVLTILNRRRFIPEIKSSNKIVKA
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHH
LGERLAMNAPIQGSAADIIKLAMVNVYNKLREKNLNSELILQVHDELILNVKKDEFDIVK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHHEECCCHHHHHHH
QLVIEEMEKAIKLNVALDVDLKYGYTWYDAK
HHHHHHHHHHHEEEEEEEEEEECCEEEECCC
>Mature Secondary Structure
MKRLLILDSNSLMNRAFYALPPLTNDDGVNTNAIYGFTNMLFKMKDEINPDNIIATFDKK
CCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCC
SPTFRHKEYSEYKAGRKKMPPELGEQFPIIKELLKYMGIKTFEIDGFEADDIIGTISKFG
CCCCCCCHHHHHHCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHC
EENDMEVYIVTGDKDALQLASEKTKVVITKKGVTETAIYDYKAFMDEFEITPTQFIDVKG
CCCCCEEEEEECCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECC
LMGDKSDNIPGVPGVGEKTAFKLIKEYGTLEDVLKHIPEISGKKLKENLENNMEQAIFSK
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
KLATIMREVPLDINLSDIENADEVNLVELKKLSKNLKLKSVLSKYDLDNNEAELQEIANI
HHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCC
EISKIETIEEMEEVLLSIKDRSYILFTLNDASKYSTIELEYLILGEEGRSHVIDFKLVSM
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEEEEECC
SNREKALNLLRTFMEDKTVKKVIHDSKNLITFLNKYDIDIQGFDFDTAIAAYLIDSSKAN
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCCC
YEVFDLINRFLEKDIKSDCDDLKAICSSYLTKLYEKLKERLHKEDMDELYYKVEHPLIYV
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
LSSMESIGFKINKDKLEELRIKFKEQIEKTEKEIYELADEEFNVSSPKQLGKILFEKLDL
HHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
PVIKKTKTGYSTNQEVLEKLIDKHPIITKVMYYRQITKINSTYVEGLKNVIDIDGCIHSN
CEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCC
FNQTVTTTGRLSSTEPNLQNIPIKYEMGKEIRKVFIPQDSDDILISCDYSQIELRVLAHV
CCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHH
ADDKNMIDAFEHHSDIHTKTASEVFKVPVDEVTSTMRSNAKAVNFGIVYGISDFSLAQDL
HCCCHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHH
KITKKEAAEYMAIYFERYPKIKEYLNYVSEYAKEKGYVLTILNRRRFIPEIKSSNKIVKA
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHH
LGERLAMNAPIQGSAADIIKLAMVNVYNKLREKNLNSELILQVHDELILNVKKDEFDIVK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHEEHHHHHEECCCHHHHHHH
QLVIEEMEKAIKLNVALDVDLKYGYTWYDAK
HHHHHHHHHHHEEEEEEEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]