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The map label for this gene is ctfB [H]

Identifier: 187933378

GI number: 187933378

Start: 2231293

End: 2231958

Strand: Reverse

Name: ctfB [H]

Synonym: CLL_A2132

Alternate gene names: 187933378

Gene position: 2231958-2231293 (Counterclockwise)

Preceding gene: 187934410

Following gene: 187933749

Centisome position: 58.73

GC content: 34.38

Gene sequence:

>666_bases
GTGATTACAGATAGTAAATTAGCAAGACAAATAATTGCAAAAAGGGTTGCAAAGGAATTAGAGGACGGACAACTTGTGAA
CCTTGGAATAGGACTTCCAACATTGGTTGCAAATTATGTACCAAAAGACAAGCATGTATTCTTTCAATCTGAAAATGGAA
TGATTGGAATGGGTGCAGTACCAACATCAGGACATGAAGATGCAAATATAATAAATGCTGGTGGACAATGTGTAACAGCA
TTACCAGAAGCAGCATTCTTTGATAGTTCTATGTCCTTTGCATTAATTAGAGGTGGACATGTTGATGCTACTGTACTTGG
AGCTTTAGAGGTAGATGAGGAAGGTAGTATAGCTAATTGGATTATTCCAGGTAAAATGGTGCCAGGTATGGGTGGAGCTA
TGGATTTAGTAACAGGTGCTAAAAAAGTAATTGTAGCCATGCAGCATACTGGAAAGGGAAAACCTAAAATTCTTAAAAAA
TGTAGATTACCATTAACTGCAAAATCACAAGTAAACTTAATTATTACAGAACTTTGTGTAATTGAAGTAACAGATAAGGG
ATTATTTTTAAGGGAGATTAGTGATTATACAACAATCGATGAAATTAAGTCTTTAACAGAAGCAAATTTAATTATTCCAG
ATGATATAAAACGTATGGCTGTATAA

Upstream 100 bases:

>100_bases
AATGGAAGCTGAAAATTTAGTTGAATTTGAAGAATTAAATAGGGAAGATATAATGACTCCAGGTGTATTAGTAAATTATA
TAATAAGGGAGGAAATTTAA

Downstream 100 bases:

>100_bases
AATTTATAGAATGATGGCTTTTTTATGAGAGTTTAGATAATTTTATATAAAAACAAATTATCCACTATTTAGTTTGGATT
TATGTTTATATATTTAGACA

Product: 3-oxoacid CoA-transferase, B subunit

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MITDSKLARQIIAKRVAKELEDGQLVNLGIGLPTLVANYVPKDKHVFFQSENGMIGMGAVPTSGHEDANIINAGGQCVTA
LPEAAFFDSSMSFALIRGGHVDATVLGALEVDEEGSIANWIIPGKMVPGMGGAMDLVTGAKKVIVAMQHTGKGKPKILKK
CRLPLTAKSQVNLIITELCVIEVTDKGLFLREISDYTTIDEIKSLTEANLIIPDDIKRMAV

Sequences:

>Translated_221_residues
MITDSKLARQIIAKRVAKELEDGQLVNLGIGLPTLVANYVPKDKHVFFQSENGMIGMGAVPTSGHEDANIINAGGQCVTA
LPEAAFFDSSMSFALIRGGHVDATVLGALEVDEEGSIANWIIPGKMVPGMGGAMDLVTGAKKVIVAMQHTGKGKPKILKK
CRLPLTAKSQVNLIITELCVIEVTDKGLFLREISDYTTIDEIKSLTEANLIIPDDIKRMAV
>Mature_221_residues
MITDSKLARQIIAKRVAKELEDGQLVNLGIGLPTLVANYVPKDKHVFFQSENGMIGMGAVPTSGHEDANIINAGGQCVTA
LPEAAFFDSSMSFALIRGGHVDATVLGALEVDEEGSIANWIIPGKMVPGMGGAMDLVTGAKKVIVAMQHTGKGKPKILKK
CRLPLTAKSQVNLIITELCVIEVTDKGLFLREISDYTTIDEIKSLTEANLIIPDDIKRMAV

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=207, Percent_Identity=52.1739130434783, Blast_Score=207, Evalue=6e-54,
Organism=Homo sapiens, GI11545841, Length=215, Percent_Identity=48.8372093023256, Blast_Score=198, Evalue=3e-51,
Organism=Escherichia coli, GI1788552, Length=211, Percent_Identity=56.3981042654028, Blast_Score=247, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=51.1627906976744, Blast_Score=207, Evalue=4e-54,
Organism=Drosophila melanogaster, GI21358325, Length=219, Percent_Identity=48.8584474885845, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24655794, Length=219, Percent_Identity=48.8584474885845, Blast_Score=211, Evalue=3e-55,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23641; Mature: 23641

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDSKLARQIIAKRVAKELEDGQLVNLGIGLPTLVANYVPKDKHVFFQSENGMIGMGAV
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEEECCC
PTSGHEDANIINAGGQCVTALPEAAFFDSSMSFALIRGGHVDATVLGALEVDEEGSIANW
CCCCCCCCEEEECCCCEEECCCCHHHHCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEE
IIPGKMVPGMGGAMDLVTGAKKVIVAMQHTGKGKPKILKKCRLPLTAKSQVNLIITELCV
EECCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCHHEEEEEEEE
IEVTDKGLFLREISDYTTIDEIKSLTEANLIIPDDIKRMAV
EEECCCCEEEEHHCCCCCHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure
MITDSKLARQIIAKRVAKELEDGQLVNLGIGLPTLVANYVPKDKHVFFQSENGMIGMGAV
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCEEEEECCCCEEEECCC
PTSGHEDANIINAGGQCVTALPEAAFFDSSMSFALIRGGHVDATVLGALEVDEEGSIANW
CCCCCCCCEEEECCCCEEECCCCHHHHCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEE
IIPGKMVPGMGGAMDLVTGAKKVIVAMQHTGKGKPKILKKCRLPLTAKSQVNLIITELCV
EECCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCHHEEEEEEEE
IEVTDKGLFLREISDYTTIDEIKSLTEANLIIPDDIKRMAV
EEECCCCEEEEHHCCCCCHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]