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The map label for this gene is yxeP [H]

Identifier: 187933336

GI number: 187933336

Start: 3484242

End: 3485420

Strand: Reverse

Name: yxeP [H]

Synonym: CLL_A3313

Alternate gene names: 187933336

Gene position: 3485420-3484242 (Counterclockwise)

Preceding gene: 187933889

Following gene: 187932832

Centisome position: 91.71

GC content: 30.2

Gene sequence:

>1179_bases
ATGATTAATGATAAGTTGTTAGAGAAAATAGGTAATTTAGAAGAGGAACTTATAAACTTATATCATATTTTACATCAAAA
TCCAGAACTATCAAATGAAGAGTTTGAAACTACGAATATGATAAAGAAATTATTACAAGAAGTAGAGATAGATATTTTAC
ATACTGATTTAAAGACAGGCCTTATAGCTGAAGTTAAAGGGAATCCAAATGGACCAATAGTTGCAATAAGATGCGATATA
GATGCGTTACCTATAAATGAGGAAAATAGTTTATCTTATAAATCTAAAAATAATGGTAAAATGCATGCATGTGGTCATGA
TTTTCATATGGCAGCAATTTTAGGTGCAGCTTATTTAATAAAGAAAAGCCAAAGAGATTTAATTGGAACAGTTAGATTTA
TATTTGAGCCTAGTGAAGAAAGCTCAAATGGGGCAAAAAAAATAATTAATACAGGTGCTCTAGAAAATGTAGAGGCTATT
TTTGGTTTGCATAATGTTCCTAATTTAGACGTTGGTGTGATGGGAATAAAACAAGGAGCAATGACAGCTGCGGTGGATAG
ATTTGAAATTAAAGTATTGGGTCGTGGTAGTCATGCAGCAAAGCCAGAAAAAAGTGTTGATCCAATTATAATATCATCAA
ATATTATAAATGCTTTACAAACTATTATAAGTAGAAATGTAAATCCAATTAATGATGCACTTTTAAGTATAACTCATATA
GAAAGCGGAAATTCATGGAATATAATACCTGACTATGCTTATATGGAAGGGACTGTTAGAACCTTAGATAAAGAAACAAG
AAATCTGATTCCTAAAAGAATGAGTAATATTATTAATGGAATAGCAGAATCATTCGGAGGAAAGGCTGAATTTATATGGC
ATGAGGGACACCCTGCTACGAATAATGAAAAAGAATGGGTAAGTATTGCATCACAGATAGGAAGAAGAATGGGATATAAA
GTAGGTGAAAATTCAATGGGATTAGAAGGTGAAGATTTTGCTTATTATCAAGAAACTATTCCAGGTGCATTTATAATTTT
TGGAATAGGTAAATCAATTGCACATCATAATCCTAAATTTTCTGTAGATGAAAATGCTATATTAAATTGTGCAAAATATT
TTGCAAATTTATCAGAAGAAGCATTATCTATATTAGTTAAGAAAAGACACAAATCATAG

Upstream 100 bases:

>100_bases
TAAAATAATATAATTATAACAAATCAATTTATATATTAGTATATATTTACATAAACAAAATGGATGTTATAATTTAAGGT
ATATTATAGGGGGATTAATA

Downstream 100 bases:

>100_bases
AAGTTATAAGAATAATACCTTAATAAATTTAGAATACTAAGTTTATTAAGGTATTTTTTATTTTTTAAGAATTTATATTA
TTAAAAAATTTGTGGATAAT

Product: thermostable carboxypeptidase 1

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MINDKLLEKIGNLEEELINLYHILHQNPELSNEEFETTNMIKKLLQEVEIDILHTDLKTGLIAEVKGNPNGPIVAIRCDI
DALPINEENSLSYKSKNNGKMHACGHDFHMAAILGAAYLIKKSQRDLIGTVRFIFEPSEESSNGAKKIINTGALENVEAI
FGLHNVPNLDVGVMGIKQGAMTAAVDRFEIKVLGRGSHAAKPEKSVDPIIISSNIINALQTIISRNVNPINDALLSITHI
ESGNSWNIIPDYAYMEGTVRTLDKETRNLIPKRMSNIINGIAESFGGKAEFIWHEGHPATNNEKEWVSIASQIGRRMGYK
VGENSMGLEGEDFAYYQETIPGAFIIFGIGKSIAHHNPKFSVDENAILNCAKYFANLSEEALSILVKKRHKS

Sequences:

>Translated_392_residues
MINDKLLEKIGNLEEELINLYHILHQNPELSNEEFETTNMIKKLLQEVEIDILHTDLKTGLIAEVKGNPNGPIVAIRCDI
DALPINEENSLSYKSKNNGKMHACGHDFHMAAILGAAYLIKKSQRDLIGTVRFIFEPSEESSNGAKKIINTGALENVEAI
FGLHNVPNLDVGVMGIKQGAMTAAVDRFEIKVLGRGSHAAKPEKSVDPIIISSNIINALQTIISRNVNPINDALLSITHI
ESGNSWNIIPDYAYMEGTVRTLDKETRNLIPKRMSNIINGIAESFGGKAEFIWHEGHPATNNEKEWVSIASQIGRRMGYK
VGENSMGLEGEDFAYYQETIPGAFIIFGIGKSIAHHNPKFSVDENAILNCAKYFANLSEEALSILVKKRHKS
>Mature_392_residues
MINDKLLEKIGNLEEELINLYHILHQNPELSNEEFETTNMIKKLLQEVEIDILHTDLKTGLIAEVKGNPNGPIVAIRCDI
DALPINEENSLSYKSKNNGKMHACGHDFHMAAILGAAYLIKKSQRDLIGTVRFIFEPSEESSNGAKKIINTGALENVEAI
FGLHNVPNLDVGVMGIKQGAMTAAVDRFEIKVLGRGSHAAKPEKSVDPIIISSNIINALQTIISRNVNPINDALLSITHI
ESGNSWNIIPDYAYMEGTVRTLDKETRNLIPKRMSNIINGIAESFGGKAEFIWHEGHPATNNEKEWVSIASQIGRRMGYK
VGENSMGLEGEDFAYYQETIPGAFIIFGIGKSIAHHNPKFSVDENAILNCAKYFANLSEEALSILVKKRHKS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=333, Percent_Identity=26.7267267267267, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 43380; Mature: 43380

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDKLLEKIGNLEEELINLYHILHQNPELSNEEFETTNMIKKLLQEVEIDILHTDLKTG
CCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCC
LIAEVKGNPNGPIVAIRCDIDALPINEENSLSYKSKNNGKMHACGHDFHMAAILGAAYLI
EEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KKSQRDLIGTVRFIFEPSEESSNGAKKIINTGALENVEAIFGLHNVPNLDVGVMGIKQGA
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCC
MTAAVDRFEIKVLGRGSHAAKPEKSVDPIIISSNIINALQTIISRNVNPINDALLSITHI
HHHHHCEEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHEEEEEE
ESGNSWNIIPDYAYMEGTVRTLDKETRNLIPKRMSNIINGIAESFGGKAEFIWHEGHPAT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
NNEKEWVSIASQIGRRMGYKVGENSMGLEGEDFAYYQETIPGAFIIFGIGKSIAHHNPKF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHHHCCCCCC
SVDENAILNCAKYFANLSEEALSILVKKRHKS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINDKLLEKIGNLEEELINLYHILHQNPELSNEEFETTNMIKKLLQEVEIDILHTDLKTG
CCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCC
LIAEVKGNPNGPIVAIRCDIDALPINEENSLSYKSKNNGKMHACGHDFHMAAILGAAYLI
EEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KKSQRDLIGTVRFIFEPSEESSNGAKKIINTGALENVEAIFGLHNVPNLDVGVMGIKQGA
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCC
MTAAVDRFEIKVLGRGSHAAKPEKSVDPIIISSNIINALQTIISRNVNPINDALLSITHI
HHHHHCEEEEEEEECCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHEEEEEE
ESGNSWNIIPDYAYMEGTVRTLDKETRNLIPKRMSNIINGIAESFGGKAEFIWHEGHPAT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
NNEKEWVSIASQIGRRMGYKVGENSMGLEGEDFAYYQETIPGAFIIFGIGKSIAHHNPKF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCHHHHCCCCCC
SVDENAILNCAKYFANLSEEALSILVKKRHKS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]