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The map label for this gene is pflD [H]

Identifier: 187932402

GI number: 187932402

Start: 1930744

End: 1933155

Strand: Reverse

Name: pflD [H]

Synonym: CLL_A1841

Alternate gene names: 187932402

Gene position: 1933155-1930744 (Counterclockwise)

Preceding gene: 187933420

Following gene: 187933302

Centisome position: 50.87

GC content: 30.18

Gene sequence:

>2412_bases
ATGGATATATTAGCAAAAGGATTTATAAATCCTACTAAAAGGGTAGAAAAATTAAAAGAAGAAATATTTTCAGCAATTCC
ATGTATTGAAGCTGATAGAGCAATAATATTAACTGAATCATTTAAAGAAACAGAAAATGAACCTATAATTATTCGAAAGG
CTAAATCTCTTGAGAAGATACTAAGTGAAATACCTGTAGTAATTAGAGATAATGAACTTATTGTAGGAAGCTTAACTAAG
AATCCTAGATCATGTCAAGTTTTTCCTGAATTTTCAAATCAATGGTTAGTTGATGAATTTGATAAATTTGAGAAAAGAAC
GTCAGATTTATTCAAAATTCCAGAAGAAACAAAATCTAAGCTTAAAGAAGTTTTTAAGTATTGGCAAGGAAAAACAGTAA
GCGAATTAGCTACATCTTATATGTCTAATGAAACTAAAGATGCTATGAATACAAAAGTATTTACAGTTGCTAATTATCAT
TTTAATGGTTTAGGTCATATTTCTGTTGATTATCCTAAAGTACTAGAAAAAGGATTTTTAGGCATTATAAAAGAAACAGA
GAATGCTATAATTAATGCTGATAAAAGCAATCCTGAATATGTTAAAAAGAAAGTATTCTGGGATTCTATAATAATTTCAT
GTAAGGCAGCAATAAAATATGCAAATAGGTATAGTGAATTAGCTAAAAAATTAGCAGGCGAAACACTAGATATAAATAGA
AAAAATGAATTATTAAAAATAGCTGAAATCTGTAGTAAAGTTCCAGCTAATCCAGCAGAAACCTTTTATGAAGCATGTCA
ATCATTTTGGTTTGTACAAGCTATAATTAGCTTAGAATCTAACGGACATGCTATATCTCCAGCACGTTTTGACCAATATA
TGTATCCTTATTATAAGAATGATATTGACAATAAATTTGCAACTAAAGAAGTAAACACAGAAATTCTTCACTGTTTGTGG
GTTAAATTTAATGACCTTACTAAAGTTCGTGATGAAACAACAACTAAAGCTTTTAGTGGTTATTCTATGTTTCAAAACTT
AATTGTAGGTGGACAAACTCCAGATGGAAAAGATGCAACAAACGAATTATCTTATATGTGTCTTGAAGCTACAGGAAGTT
TAAAATTACCACAACCATCTCTTTGTGTTAGAATATGGAGCAAAACTCCTGATGAATTTTTAATTAGAACTTGTGAATTA
ACTCGTTTAGGAACTGGATTGCCAGCATTTTACAATGATGAAGTGGTTATTCCAACCTTGATTAATCAAGGTTTAACAAT
AGAAGATGCTAGAGATTATGCAATTGTAGGATGCGTTGAACCTCAAAAGCCTGGAAAAACTGATGGCTGGTATGATGCTG
CATTCTTTAACTTAGCAAAGATATTAGAACTATCTATGAATAATGGTAGACTTAATGGTAGACAAGTTGGGCCTGAAACT
GGTGAATTTACTTCATTTAAAAATATAGATGATTTTATAAATGCATATAAAAAACAAATAGAATATTTTGTATTCCATAT
GGTTGCAGCAGATAATTGTGTTGATATAGCACATGCTGAAAGGGCGCCACTCCCATTTTTATCATCTATGCTTGATGATT
GTATTGGCACAGGAAAGTCAATTCAAGAAGGGGGAGGACACTATCGTTTTTCAGGACCTCTAGGTGTTGGAATTGCAAAT
GTTGGTGATTCATTCATGGCTATAAAGAAGCTTATATTTGATGAAAACAAAATTACTTTATTAAAATTAAAATCTGCTGT
TGATAGTAATTTTGGTGAGAATGAAGATGATCCAATTAAAAAAGCAGAATATGAAGATATAAAACAATTAATTTTAAATA
GAGTTCCTAAGTTTGGTAATGATATAGATGAAGTAGATGAATTTACACGTGATGGTGCACTTATTTATTGTAAGGAAGTA
TTAAAATATACAAATCAACGTGGAGGAAAATTCATTCCAGGATTATACCCAGTTTCAAATAATGTATATTTAGGAAGTCT
TGTTGGTGCAACACCTGATGGTAGAAGTGCATTTAAACCTTTAGCAGATGGAGTATCTCCAACTAGAGGTGCAGATGTTA
ATGGACCAACAGCTGCTGCAAATTCAGTATCTAAATTAGAACATTTTGCAGCTCCAAGTGGAACTTTATTCAATCAAAAG
TTCAATCCAAATTCTCTACAAGGAGATAATGGATTAAAAAACTTAGGATCTTTAATAAGAAGTTATTTTGATAGAAAAGG
TATGCATATACAATTTAATGTAATAGATAAAAAAGTTCTTGTTGAAGCTCAAAAACATCCTGAACAATATAGAGATTTAA
TAGTACGTGTTGCTGGTTATAGTGCTCAATTTATTTGTTTAGATAAAGGCGTACAAGATGATATTATAAAGAGAACAGAA
CAAAATTTATAA

Upstream 100 bases:

>100_bases
GAAATAGATTATTTGAGCTAAATCAAGTAAACGTATTCCTATGAAAAATCACATTACTTAGCTTACAAATAAAATATCTT
GTAATAAGAGGGGGAGAACT

Downstream 100 bases:

>100_bases
ATAAGTTACTATAAGTTTAAATATAGAAATATAAATATTCTTATGAAAGGAAAAAGCTATGCTAAAAAACGAAGTGGATT
TAAATAAAATGGGTACTGTT

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MDILAKGFINPTKRVEKLKEEIFSAIPCIEADRAIILTESFKETENEPIIIRKAKSLEKILSEIPVVIRDNELIVGSLTK
NPRSCQVFPEFSNQWLVDEFDKFEKRTSDLFKIPEETKSKLKEVFKYWQGKTVSELATSYMSNETKDAMNTKVFTVANYH
FNGLGHISVDYPKVLEKGFLGIIKETENAIINADKSNPEYVKKKVFWDSIIISCKAAIKYANRYSELAKKLAGETLDINR
KNELLKIAEICSKVPANPAETFYEACQSFWFVQAIISLESNGHAISPARFDQYMYPYYKNDIDNKFATKEVNTEILHCLW
VKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPDGKDATNELSYMCLEATGSLKLPQPSLCVRIWSKTPDEFLIRTCEL
TRLGTGLPAFYNDEVVIPTLINQGLTIEDARDYAIVGCVEPQKPGKTDGWYDAAFFNLAKILELSMNNGRLNGRQVGPET
GEFTSFKNIDDFINAYKKQIEYFVFHMVAADNCVDIAHAERAPLPFLSSMLDDCIGTGKSIQEGGGHYRFSGPLGVGIAN
VGDSFMAIKKLIFDENKITLLKLKSAVDSNFGENEDDPIKKAEYEDIKQLILNRVPKFGNDIDEVDEFTRDGALIYCKEV
LKYTNQRGGKFIPGLYPVSNNVYLGSLVGATPDGRSAFKPLADGVSPTRGADVNGPTAAANSVSKLEHFAAPSGTLFNQK
FNPNSLQGDNGLKNLGSLIRSYFDRKGMHIQFNVIDKKVLVEAQKHPEQYRDLIVRVAGYSAQFICLDKGVQDDIIKRTE
QNL

Sequences:

>Translated_803_residues
MDILAKGFINPTKRVEKLKEEIFSAIPCIEADRAIILTESFKETENEPIIIRKAKSLEKILSEIPVVIRDNELIVGSLTK
NPRSCQVFPEFSNQWLVDEFDKFEKRTSDLFKIPEETKSKLKEVFKYWQGKTVSELATSYMSNETKDAMNTKVFTVANYH
FNGLGHISVDYPKVLEKGFLGIIKETENAIINADKSNPEYVKKKVFWDSIIISCKAAIKYANRYSELAKKLAGETLDINR
KNELLKIAEICSKVPANPAETFYEACQSFWFVQAIISLESNGHAISPARFDQYMYPYYKNDIDNKFATKEVNTEILHCLW
VKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPDGKDATNELSYMCLEATGSLKLPQPSLCVRIWSKTPDEFLIRTCEL
TRLGTGLPAFYNDEVVIPTLINQGLTIEDARDYAIVGCVEPQKPGKTDGWYDAAFFNLAKILELSMNNGRLNGRQVGPET
GEFTSFKNIDDFINAYKKQIEYFVFHMVAADNCVDIAHAERAPLPFLSSMLDDCIGTGKSIQEGGGHYRFSGPLGVGIAN
VGDSFMAIKKLIFDENKITLLKLKSAVDSNFGENEDDPIKKAEYEDIKQLILNRVPKFGNDIDEVDEFTRDGALIYCKEV
LKYTNQRGGKFIPGLYPVSNNVYLGSLVGATPDGRSAFKPLADGVSPTRGADVNGPTAAANSVSKLEHFAAPSGTLFNQK
FNPNSLQGDNGLKNLGSLIRSYFDRKGMHIQFNVIDKKVLVEAQKHPEQYRDLIVRVAGYSAQFICLDKGVQDDIIKRTE
QNL
>Mature_803_residues
MDILAKGFINPTKRVEKLKEEIFSAIPCIEADRAIILTESFKETENEPIIIRKAKSLEKILSEIPVVIRDNELIVGSLTK
NPRSCQVFPEFSNQWLVDEFDKFEKRTSDLFKIPEETKSKLKEVFKYWQGKTVSELATSYMSNETKDAMNTKVFTVANYH
FNGLGHISVDYPKVLEKGFLGIIKETENAIINADKSNPEYVKKKVFWDSIIISCKAAIKYANRYSELAKKLAGETLDINR
KNELLKIAEICSKVPANPAETFYEACQSFWFVQAIISLESNGHAISPARFDQYMYPYYKNDIDNKFATKEVNTEILHCLW
VKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPDGKDATNELSYMCLEATGSLKLPQPSLCVRIWSKTPDEFLIRTCEL
TRLGTGLPAFYNDEVVIPTLINQGLTIEDARDYAIVGCVEPQKPGKTDGWYDAAFFNLAKILELSMNNGRLNGRQVGPET
GEFTSFKNIDDFINAYKKQIEYFVFHMVAADNCVDIAHAERAPLPFLSSMLDDCIGTGKSIQEGGGHYRFSGPLGVGIAN
VGDSFMAIKKLIFDENKITLLKLKSAVDSNFGENEDDPIKKAEYEDIKQLILNRVPKFGNDIDEVDEFTRDGALIYCKEV
LKYTNQRGGKFIPGLYPVSNNVYLGSLVGATPDGRSAFKPLADGVSPTRGADVNGPTAAANSVSKLEHFAAPSGTLFNQK
FNPNSLQGDNGLKNLGSLIRSYFDRKGMHIQFNVIDKKVLVEAQKHPEQYRDLIVRVAGYSAQFICLDKGVQDDIIKRTE
QNL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=798, Percent_Identity=39.8496240601504, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI1787044, Length=812, Percent_Identity=35.4679802955665, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI48994926, Length=580, Percent_Identity=25.6896551724138, Blast_Score=158, Evalue=2e-39,
Organism=Escherichia coli, GI1787131, Length=561, Percent_Identity=26.3814616755793, Blast_Score=153, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90111; Mature: 90111

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILAKGFINPTKRVEKLKEEIFSAIPCIEADRAIILTESFKETENEPIIIRKAKSLEKI
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCEEEECHHHHHHH
LSEIPVVIRDNELIVGSLTKNPRSCQVFPEFSNQWLVDEFDKFEKRTSDLFKIPEETKSK
HHHCCEEEECCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LKEVFKYWQGKTVSELATSYMSNETKDAMNTKVFTVANYHFNGLGHISVDYPKVLEKGFL
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCEEEEEEEEEECCCEEEECCHHHHHHHHHH
GIIKETENAIINADKSNPEYVKKKVFWDSIIISCKAAIKYANRYSELAKKLAGETLDINR
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KNELLKIAEICSKVPANPAETFYEACQSFWFVQAIISLESNGHAISPARFDQYMYPYYKN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHC
DIDNKFATKEVNTEILHCLWVKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPDGKDAT
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
NELSYMCLEATGSLKLPQPSLCVRIWSKTPDEFLIRTCELTRLGTGLPAFYNDEVVIPTL
HHHHEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHH
INQGLTIEDARDYAIVGCVEPQKPGKTDGWYDAAFFNLAKILELSMNNGRLNGRQVGPET
HCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GEFTSFKNIDDFINAYKKQIEYFVFHMVAADNCVDIAHAERAPLPFLSSMLDDCIGTGKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCC
IQEGGGHYRFSGPLGVGIANVGDSFMAIKKLIFDENKITLLKLKSAVDSNFGENEDDPIK
HHCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCH
KAEYEDIKQLILNRVPKFGNDIDEVDEFTRDGALIYCKEVLKYTNQRGGKFIPGLYPVSN
HCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCC
NVYLGSLVGATPDGRSAFKPLADGVSPTRGADVNGPTAAANSVSKLEHFAAPSGTLFNQK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCC
FNPNSLQGDNGLKNLGSLIRSYFDRKGMHIQFNVIDKKVLVEAQKHPEQYRDLIVRVAGY
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHCCC
SAQFICLDKGVQDDIIKRTEQNL
CEEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MDILAKGFINPTKRVEKLKEEIFSAIPCIEADRAIILTESFKETENEPIIIRKAKSLEKI
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHCCCCCEEEECHHHHHHH
LSEIPVVIRDNELIVGSLTKNPRSCQVFPEFSNQWLVDEFDKFEKRTSDLFKIPEETKSK
HHHCCEEEECCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LKEVFKYWQGKTVSELATSYMSNETKDAMNTKVFTVANYHFNGLGHISVDYPKVLEKGFL
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCEEEEEEEEEECCCEEEECCHHHHHHHHHH
GIIKETENAIINADKSNPEYVKKKVFWDSIIISCKAAIKYANRYSELAKKLAGETLDINR
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KNELLKIAEICSKVPANPAETFYEACQSFWFVQAIISLESNGHAISPARFDQYMYPYYKN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHC
DIDNKFATKEVNTEILHCLWVKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPDGKDAT
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
NELSYMCLEATGSLKLPQPSLCVRIWSKTPDEFLIRTCELTRLGTGLPAFYNDEVVIPTL
HHHHEEEEECCCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHH
INQGLTIEDARDYAIVGCVEPQKPGKTDGWYDAAFFNLAKILELSMNNGRLNGRQVGPET
HCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GEFTSFKNIDDFINAYKKQIEYFVFHMVAADNCVDIAHAERAPLPFLSSMLDDCIGTGKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCC
IQEGGGHYRFSGPLGVGIANVGDSFMAIKKLIFDENKITLLKLKSAVDSNFGENEDDPIK
HHCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCH
KAEYEDIKQLILNRVPKFGNDIDEVDEFTRDGALIYCKEVLKYTNQRGGKFIPGLYPVSN
HCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCC
NVYLGSLVGATPDGRSAFKPLADGVSPTRGADVNGPTAAANSVSKLEHFAAPSGTLFNQK
CEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCC
FNPNSLQGDNGLKNLGSLIRSYFDRKGMHIQFNVIDKKVLVEAQKHPEQYRDLIVRVAGY
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHCCC
SAQFICLDKGVQDDIIKRTEQNL
CEEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]