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The map label for this gene is cusS [H]

Identifier: 187928800

GI number: 187928800

Start: 1810752

End: 1812149

Strand: Reverse

Name: cusS [H]

Synonym: Rpic_1717

Alternate gene names: 187928800

Gene position: 1812149-1810752 (Counterclockwise)

Preceding gene: 187928801

Following gene: 187928798

Centisome position: 45.96

GC content: 62.09

Gene sequence:

>1398_bases
ATGGGGCAGTTCTCCCTGACCACTCGGCTCACCGCGTATTTCTCGCTGTGTTCGGCAGCCGTCTTGCTGGGCCTGGGCGT
GGTGATTGCGCTGGCCATGGATCAGCACTTTGCGGTGGAGGACTACACCGCGCTGCGCGAGAACGTCAGCGTCATTGAAA
AGATTGTCGAATCGAGTCCCGCCGAACAGGTCCCCGAACGCATCCGCGAGGCAATCCAACATCGCACCGATCTCGTAGCT
CGTATCCAGGGTTCGGACGGGCACATGCTGTACGTAACCCAGGATTTCGATTTTCAAGCGGCAACGCAAGCGCTGGCGCA
GTTGCGGCATACCAGCGAAACCCTCGTTTGGGCGCAAGGCGGTCAGCAATATCGGGGCATGCACGCGGCCATCCCACTGC
GAGATGGATCATCGGGCGAGCTTGATGTCCTGCTGGGGATCAACACGGACATTCACGCCCACTTCCTCCACACCTTCCGC
GGGACGCTCGCTTTCTATATCGCCGTCGCAGCGCTGGCCACGGGCCTATTCGGCTGGTGGGCTGCACGCCGAGGCCTGGC
GCCTTTGCGCACCATGGCCTCGCGGGCGCGCACGGTGACTGCCGACAAGCTCGATGAACGCATGCCTGTTGATACCGTGC
CAGTCGAAGTGGCGGACCTTGCCGCGACCCTGAACGGCATGTTGGAGCGCCTGCAGAACGATTTCCGCCGCTTGTCGGAC
TTCTCGACCGACATCGCCCATGAACTGCGTACGCCTATCACGAACCTGCTCACGCAGACGGAGGTCGTGCTATCCCAGCC
GCGCGAGGGCACGAAGTATCGCGATGTGCTGACCTCCAATGCGGAGGAACTGCAGCGCCTGGCACGCATGGTGTCCGACA
TGCTGTACCTCGCCAAGATGGAGCACAGCCTGACGCTGCCAAGCGCCGAAGACATCCGCATCGCTGACGAAATCCGCGCG
TTGTTCGATTTCTATGACGCGCTTGCAGAAGACAAGGCTGTCCTGCTCGAAACGCGGGGCGATGGACACGTCACAGGAGA
CCGGCTCATGCTGCGCCGTGCGTTGAGCAACCTGCTCTCCAACGCCATCCGGCACACCCCGCGACATGGCCGCGTGGTCG
TCTCGGTCAGTGATGATCGCGACGGCATGACGGTTTCGGTAGACAACGACGGCAGCGAGATCGCGCCGCAGCTGCTGCCA
TTTATCTTCGAGCGCTTTTTCCGGGCGGACAAATCGCGCGCGCGGCCCGAGTCGGAAAGTGCCGGCCTTGGCTTGGCCAT
CACCAAAGCGATCGTTGCGGCGCACGGCGGCACCATCACCGTTGCGCGCATTGACGGGCGCACACGGTTCTCTATCCGCC
TACCGCGTTGCATCGATGCCGCAATATTGTCTGTATAG

Upstream 100 bases:

>100_bases
GCCGGTTGCGCGCCAAGATCGACGACCACTTCGAGCCGAAGCTGATCCAAACGGTACGCGGCATGGGTTATGTACTGGAA
GCGCCCGAGGACGCGGCCTG

Downstream 100 bases:

>100_bases
TTCTTTGCACACTGCGGCGACTACTGCCAAGCCGGGCTTGGCGGGCATTTTGGGTCGCAAACCGAAACAGCCGCCGGTTT
CGCGCGCCACTCCGATGGAC

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGQFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPAEQVPERIREAIQHRTDLVA
RIQGSDGHMLYVTQDFDFQAATQALAQLRHTSETLVWAQGGQQYRGMHAAIPLRDGSSGELDVLLGINTDIHAHFLHTFR
GTLAFYIAVAALATGLFGWWAARRGLAPLRTMASRARTVTADKLDERMPVDTVPVEVADLAATLNGMLERLQNDFRRLSD
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRA
LFDFYDALAEDKAVLLETRGDGHVTGDRLMLRRALSNLLSNAIRHTPRHGRVVVSVSDDRDGMTVSVDNDGSEIAPQLLP
FIFERFFRADKSRARPESESAGLGLAITKAIVAAHGGTITVARIDGRTRFSIRLPRCIDAAILSV

Sequences:

>Translated_465_residues
MGQFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPAEQVPERIREAIQHRTDLVA
RIQGSDGHMLYVTQDFDFQAATQALAQLRHTSETLVWAQGGQQYRGMHAAIPLRDGSSGELDVLLGINTDIHAHFLHTFR
GTLAFYIAVAALATGLFGWWAARRGLAPLRTMASRARTVTADKLDERMPVDTVPVEVADLAATLNGMLERLQNDFRRLSD
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRA
LFDFYDALAEDKAVLLETRGDGHVTGDRLMLRRALSNLLSNAIRHTPRHGRVVVSVSDDRDGMTVSVDNDGSEIAPQLLP
FIFERFFRADKSRARPESESAGLGLAITKAIVAAHGGTITVARIDGRTRFSIRLPRCIDAAILSV
>Mature_464_residues
GQFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPAEQVPERIREAIQHRTDLVAR
IQGSDGHMLYVTQDFDFQAATQALAQLRHTSETLVWAQGGQQYRGMHAAIPLRDGSSGELDVLLGINTDIHAHFLHTFRG
TLAFYIAVAALATGLFGWWAARRGLAPLRTMASRARTVTADKLDERMPVDTVPVEVADLAATLNGMLERLQNDFRRLSDF
STDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRAL
FDFYDALAEDKAVLLETRGDGHVTGDRLMLRRALSNLLSNAIRHTPRHGRVVVSVSDDRDGMTVSVDNDGSEIAPQLLPF
IFERFFRADKSRARPESESAGLGLAITKAIVAAHGGTITVARIDGRTRFSIRLPRCIDAAILSV

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=473, Percent_Identity=36.9978858350951, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788279, Length=305, Percent_Identity=31.1475409836066, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=33.9449541284404, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=30.9090909090909, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787894, Length=323, Percent_Identity=30.030959752322, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=30.1204819277108, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790861, Length=352, Percent_Identity=27.5568181818182, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790551, Length=293, Percent_Identity=25.5972696245734, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789403, Length=259, Percent_Identity=29.3436293436293, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=26.007326007326, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786912, Length=216, Percent_Identity=27.3148148148148, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=331, Percent_Identity=23.8670694864048, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=24.3589743589744, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51359; Mature: 51228

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
AEQVPERIREAIQHRTDLVARIQGSDGHMLYVTQDFDFQAATQALAQLRHTSETLVWAQG
HHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCC
GQQYRGMHAAIPLRDGSSGELDVLLGINTDIHAHFLHTFRGTLAFYIAVAALATGLFGWW
CHHHCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRGLAPLRTMASRARTVTADKLDERMPVDTVPVEVADLAATLNGMLERLQNDFRRLSD
HHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHSLTLPSAEDIRIADEIRALFDFYDALAEDKAVLLETRGDGHVTGDRLMLRRALSNLLS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
NAIRHTPRHGRVVVSVSDDRDGMTVSVDNDGSEIAPQLLPFIFERFFRADKSRARPESES
HHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGLGLAITKAIVAAHGGTITVARIDGRTRFSIRLPRCIDAAILSV
CCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
GQFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
AEQVPERIREAIQHRTDLVARIQGSDGHMLYVTQDFDFQAATQALAQLRHTSETLVWAQG
HHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEECCC
GQQYRGMHAAIPLRDGSSGELDVLLGINTDIHAHFLHTFRGTLAFYIAVAALATGLFGWW
CHHHCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARRGLAPLRTMASRARTVTADKLDERMPVDTVPVEVADLAATLNGMLERLQNDFRRLSD
HHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHSLTLPSAEDIRIADEIRALFDFYDALAEDKAVLLETRGDGHVTGDRLMLRRALSNLLS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
NAIRHTPRHGRVVVSVSDDRDGMTVSVDNDGSEIAPQLLPFIFERFFRADKSRARPESES
HHHHCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGLGLAITKAIVAAHGGTITVARIDGRTRFSIRLPRCIDAAILSV
CCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]