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The map label for this gene is ribH

Identifier: 187927757

GI number: 187927757

Start: 702407

End: 702907

Strand: Reverse

Name: ribH

Synonym: Rpic_0661

Alternate gene names: 187927757

Gene position: 702907-702407 (Counterclockwise)

Preceding gene: 187927758

Following gene: 187927756

Centisome position: 17.83

GC content: 64.87

Gene sequence:

>501_bases
ATGGACCACGGCTTCTACCCGACCAATCTCAACGGCGAAGGCCTGCGCATCGGCATCGTCCAGGCGCGTTTCAACGAGCC
CGTGTGCGAAGCACTGCGAGAAGCTTGCGTCGCTGAACTCGAACAACTCGGCGTGGCCGGCGAAGACGTGCTGCTCGTGA
CTGTGCCGGGTGCGCTGGAAGTCCCGCTGGCGCTGCAGAAGATGGCCGAGTCGGGCCAGTTCGACGCGCTGATCGCGCTG
GGCGCAGTCATCCGCGGCGAGACGTACCACTTCGAACTGGTGTCCAACGAATCGGGCGCCGGCATCACGCGCGTGGGCCT
GGACTTCAACATCGCCATCGCCAACGGCATCCTGACCACCGACACAGACGCACAGGCCCACGCCCGCACCCGCGAAAAAG
GTCGCGACTGCGCCCGCACCGCCGTCGAAATGGCCAACCTGACCAACGACCTCGACGGTCTGCAGGATCACGGCCAGGAC
GACGACGGCGAGAATGAATAA

Upstream 100 bases:

>100_bases
ATCCACCGCGACACACTGATTTTCCCGCGGCAGCCGAGACTGCCGCGCCGGCTTTCTTCTTCTGACGTTGCCCGACCGGC
AACCTTAACTGGAATACACC

Downstream 100 bases:

>100_bases
GCGCTTCCTCGCGCAAAGGCTTTTCCCATGACGCAAGACAATTCCCAAGCCAAGGCCAAAGCGCCCGCCAAGAGCGCGCG
CCGCCGTGCGCGCGAACTGG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MDHGFYPTNLNGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVGLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAVEMANLTNDLDGLQDHGQD
DDGENE

Sequences:

>Translated_166_residues
MDHGFYPTNLNGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVGLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAVEMANLTNDLDGLQDHGQD
DDGENE
>Mature_166_residues
MDHGFYPTNLNGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALEVPLALQKMAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRVGLDFNIAIANGILTTDTDAQAHARTREKGRDCARTAVEMANLTNDLDGLQDHGQD
DDGENE

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=137, Percent_Identity=42.3357664233577, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=30.4054054054054, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_RALPJ (B2U7F0)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898244.1
- ProteinModelPortal:   B2U7F0
- SMR:   B2U7F0
- GeneID:   6288301
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0661
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17721; Mature: 17721

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHGFYPTNLNGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VPLALQKMAESGQFDALIALGAVIRGETYHFELVSNESGAGITRVGLDFNIAIANGILTT
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEEEEEEECCEEEC
DTDAQAHARTREKGRDCARTAVEMANLTNDLDGLQDHGQDDDGENE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDHGFYPTNLNGEGLRIGIVQARFNEPVCEALREACVAELEQLGVAGEDVLLVTVPGALE
CCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VPLALQKMAESGQFDALIALGAVIRGETYHFELVSNESGAGITRVGLDFNIAIANGILTT
CCHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEEEEEEECCEEEC
DTDAQAHARTREKGRDCARTAVEMANLTNDLDGLQDHGQDDDGENE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA