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The map label for this gene is trmB

Identifier: 187927749

GI number: 187927749

Start: 691756

End: 692529

Strand: Reverse

Name: trmB

Synonym: Rpic_0653

Alternate gene names: 187927749

Gene position: 692529-691756 (Counterclockwise)

Preceding gene: 187927750

Following gene: 187927744

Centisome position: 17.57

GC content: 65.76

Gene sequence:

>774_bases
ATGCAGCCCATCGAACAGCCGGGCACCGGCCCCGACGACATCACGCCGGAATCGCAAGACACCAATACCGCAGAGAGCGC
CGAAAGCGGTGCGGAAACCGGCCACCCGCGTCGCATCCGCTCGTTCGTGCGCCGCGCCGGCCGTACGTCGACCGGCCAGC
AGCGTGCCATCAATGAACTCGGTCCGCGCTTCCAATTGCCCTACACCACAGAGCCGCTGGACTGGGACGCCGCCTTCGGC
CGCGCTGGCGCCAAGCGCATCTTCGAGATCGGCTTCGGCATGGGTGAAACCACCGCGCACATCGCGCAATTGCGCCCGGA
CGACGATTTTCTAGGCGTCGAGGTGCATGAGCCGGGCGTTGGAGCGCTGCTCAAGCTGATCGGCGAACGCGAAATCGGCA
ACATCCGTATCGTTTCGCACGATGCGGTGGAAGTGCTGGCGCAGATGATTCCGGAGGGTACGCTGGATGGCATCCACGTG
TTCTTCCCCGACCCGTGGCACAAGAAGCGCCACAACAAGCGGCGCCTGATCCAGAGCCCGTTTGTCGCACGGCTGGCGGC
GCACCTGAAACCGGGCGGCTATCTGCACTGCGCGACGGACTGGGAGGAATACGCCCACCAGATGCTGGAGGTGCTGTCCT
CCGAGCCGACGCTGGAAAACACCGCCGACGGCTTTGCACCGCGCCCCGACTACCGCCCTGTCACCAAGTTCGAGAAACGC
GGCCTGCGCCTCGGGCACGGCGTGTGGGACGTGGTCTTCCGCAAGCGCGGCTAG

Upstream 100 bases:

>100_bases
CATCGCCCGCTCCACCGAACAGTCTGAAGCCGCCCTCGGCGGCTTTTTTGTTGCCTGCGCGCCCGTCGCTCCATCGACGG
GCAACCGGCACGGGATCTCC

Downstream 100 bases:

>100_bases
CCACGTCCGCGCACGCTCGCGCAAGTCAGCGTGCGCCGAAATGCGTATCGCCGAAGAGGTTCTTGGCGTGGCGCGGGAAA
GCGCGCCAGTATTGCGGCGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQPIEQPGTGPDDITPESQDTNTAESAESGAETGHPRRIRSFVRRAGRTSTGQQRAINELGPRFQLPYTTEPLDWDAAFG
RAGAKRIFEIGFGMGETTAHIAQLRPDDDFLGVEVHEPGVGALLKLIGEREIGNIRIVSHDAVEVLAQMIPEGTLDGIHV
FFPDPWHKKRHNKRRLIQSPFVARLAAHLKPGGYLHCATDWEEYAHQMLEVLSSEPTLENTADGFAPRPDYRPVTKFEKR
GLRLGHGVWDVVFRKRG

Sequences:

>Translated_257_residues
MQPIEQPGTGPDDITPESQDTNTAESAESGAETGHPRRIRSFVRRAGRTSTGQQRAINELGPRFQLPYTTEPLDWDAAFG
RAGAKRIFEIGFGMGETTAHIAQLRPDDDFLGVEVHEPGVGALLKLIGEREIGNIRIVSHDAVEVLAQMIPEGTLDGIHV
FFPDPWHKKRHNKRRLIQSPFVARLAAHLKPGGYLHCATDWEEYAHQMLEVLSSEPTLENTADGFAPRPDYRPVTKFEKR
GLRLGHGVWDVVFRKRG
>Mature_257_residues
MQPIEQPGTGPDDITPESQDTNTAESAESGAETGHPRRIRSFVRRAGRTSTGQQRAINELGPRFQLPYTTEPLDWDAAFG
RAGAKRIFEIGFGMGETTAHIAQLRPDDDFLGVEVHEPGVGALLKLIGEREIGNIRIVSHDAVEVLAQMIPEGTLDGIHV
FFPDPWHKKRHNKRRLIQSPFVARLAAHLKPGGYLHCATDWEEYAHQMLEVLSSEPTLENTADGFAPRPDYRPVTKFEKR
GLRLGHGVWDVVFRKRG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=51.9823788546256, Blast_Score=238, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_RALPJ (B2U7E2)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898236.1
- ProteinModelPortal:   B2U7E2
- SMR:   B2U7E2
- GeneID:   6288040
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0653
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28699; Mature: 28699

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 114-114 BINDING 141-141 BINDING 164-164 BINDING 168-168 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIEQPGTGPDDITPESQDTNTAESAESGAETGHPRRIRSFVRRAGRTSTGQQRAINEL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHC
GPRFQLPYTTEPLDWDAAFGRAGAKRIFEIGFGMGETTAHIAQLRPDDDFLGVEVHEPGV
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCH
GALLKLIGEREIGNIRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWHKKRHNKRRLIQSP
HHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCH
FVARLAAHLKPGGYLHCATDWEEYAHQMLEVLSSEPTLENTADGFAPRPDYRPVTKFEKR
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GLRLGHGVWDVVFRKRG
CCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MQPIEQPGTGPDDITPESQDTNTAESAESGAETGHPRRIRSFVRRAGRTSTGQQRAINEL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHC
GPRFQLPYTTEPLDWDAAFGRAGAKRIFEIGFGMGETTAHIAQLRPDDDFLGVEVHEPGV
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEECCCCH
GALLKLIGEREIGNIRIVSHDAVEVLAQMIPEGTLDGIHVFFPDPWHKKRHNKRRLIQSP
HHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCH
FVARLAAHLKPGGYLHCATDWEEYAHQMLEVLSSEPTLENTADGFAPRPDYRPVTKFEKR
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GLRLGHGVWDVVFRKRG
CCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA