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The map label for this gene is rfaF [H]

Identifier: 187927586

GI number: 187927586

Start: 519067

End: 520092

Strand: Reverse

Name: rfaF [H]

Synonym: Rpic_0486

Alternate gene names: 187927586

Gene position: 520092-519067 (Counterclockwise)

Preceding gene: 187927587

Following gene: 187927585

Centisome position: 13.19

GC content: 67.54

Gene sequence:

>1026_bases
ATGATGCGCAAGATCCTCGTCGTCGCCCCCAACTGGATCGGTGATGCGCTGATGGCGCAGCCGCTGTTTGCGCAGCTCAA
GGCGCGGCACCCGCGCGCGCAGATCCACGCCATTGCCCCGAAATGGGTCGCGCCCGTGCTCGCGCGCATGCCGGAAATCG
CGCGCGTACTGCCCACCGAACTTGCCCACGGCAAGCTGCAGCTCGGCAGCCGCACGATGTTCGCGCAGCAGCTCAAGGGC
GAAACCTTCGACGCAGCCTATGTGCTGCCGAACTCGTTCAAGAGCGCGCTGATCCCGTGGCTGGCCGGCATTCCGCTGCG
TATCGGCTACAAGGGCGAGTCGCGGCTGGGCGTGCTGAACGTGCGCTACCCGAATCCGCCCAAGAATGCGCGTCCGCCGA
TGGTGCAGCACTACGCGGCGCTGGCTTTCAAGCCCCATGCCAAGCTGCCCGACACGCTGCCCGACCCGAAGCTGGACGTT
GATGTGCAGCGCGTGGCAGCCACGTCCGCCAAGTTCGGCATTCCTGGCAACGCGCGGCTGATTGCCTTCTGCCCCGGCGC
GGAATACGGCCCAGCAAAGCGCTGGCCGGCCGAGCACTTCGCAGAGCTGGCGCAGATGCTGCGCCGCTCGTTCCCGTACG
CGCAGATCGTCGCGCTCGGTTCGGGCAAGGACCGCGAGATGGCCAACGCCATCGTCGAGCGCGCGCCGTTCGTGCGCAAC
CTGTGCGGCGAGACCTCGCTGGATGAAGCGATCGAACTGCTCGCCCGCGCCGAAGCCGCCATCTGCAACGACTCCGGCCT
GATGCACGTGACGGCTGCCCTGGGGCGCCCGCAGGTGGCCGTGTTCGGCTCCAGCGACCCCCGCCACACGCCTCCGCTGT
CGCCTGCTGCGAGTATCATGTGGCTCCATCTGGAGTGCAGTCCGTGCTTTGCACGCGAATGCCCGCTGGGCCACTTGCGC
TGCCTGCGCGACATCGGCCCCGAGATGGTGTTTCATGAATTGCGCCGGCTGCTGCAGCCGCAGTAG

Upstream 100 bases:

>100_bases
GGCACCGTCTACAAGCTGGCTCCCGGCGTTGAACTGAAGGGCCACCATTGAGCCCGATTCACCTGCAGTACACCGGACGC
CGCGCCCGCCAGGCGATTCG

Downstream 100 bases:

>100_bases
TACGCTGTCCTTAACGTTCACCGCCATGCCACGATTTGCCCGCCTGTTCGAAGCCGCCGAGGACATCCTCGAAGCCTATC
GCGATGCGCTCAAGCGCCGC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MMRKILVVAPNWIGDALMAQPLFAQLKARHPRAQIHAIAPKWVAPVLARMPEIARVLPTELAHGKLQLGSRTMFAQQLKG
ETFDAAYVLPNSFKSALIPWLAGIPLRIGYKGESRLGVLNVRYPNPPKNARPPMVQHYAALAFKPHAKLPDTLPDPKLDV
DVQRVAATSAKFGIPGNARLIAFCPGAEYGPAKRWPAEHFAELAQMLRRSFPYAQIVALGSGKDREMANAIVERAPFVRN
LCGETSLDEAIELLARAEAAICNDSGLMHVTAALGRPQVAVFGSSDPRHTPPLSPAASIMWLHLECSPCFARECPLGHLR
CLRDIGPEMVFHELRRLLQPQ

Sequences:

>Translated_341_residues
MMRKILVVAPNWIGDALMAQPLFAQLKARHPRAQIHAIAPKWVAPVLARMPEIARVLPTELAHGKLQLGSRTMFAQQLKG
ETFDAAYVLPNSFKSALIPWLAGIPLRIGYKGESRLGVLNVRYPNPPKNARPPMVQHYAALAFKPHAKLPDTLPDPKLDV
DVQRVAATSAKFGIPGNARLIAFCPGAEYGPAKRWPAEHFAELAQMLRRSFPYAQIVALGSGKDREMANAIVERAPFVRN
LCGETSLDEAIELLARAEAAICNDSGLMHVTAALGRPQVAVFGSSDPRHTPPLSPAASIMWLHLECSPCFARECPLGHLR
CLRDIGPEMVFHELRRLLQPQ
>Mature_341_residues
MMRKILVVAPNWIGDALMAQPLFAQLKARHPRAQIHAIAPKWVAPVLARMPEIARVLPTELAHGKLQLGSRTMFAQQLKG
ETFDAAYVLPNSFKSALIPWLAGIPLRIGYKGESRLGVLNVRYPNPPKNARPPMVQHYAALAFKPHAKLPDTLPDPKLDV
DVQRVAATSAKFGIPGNARLIAFCPGAEYGPAKRWPAEHFAELAQMLRRSFPYAQIVALGSGKDREMANAIVERAPFVRN
LCGETSLDEAIELLARAEAAICNDSGLMHVTAALGRPQVAVFGSSDPRHTPPLSPAASIMWLHLECSPCFARECPLGHLR
CLRDIGPEMVFHELRRLLQPQ

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=347, Percent_Identity=44.956772334294, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 37514; Mature: 37514

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKILVVAPNWIGDALMAQPLFAQLKARHPRAQIHAIAPKWVAPVLARMPEIARVLPTE
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHH
LAHGKLQLGSRTMFAQQLKGETFDAAYVLPNSFKSALIPWLAGIPLRIGYKGESRLGVLN
HCCCEEECCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCCCEEEEE
VRYPNPPKNARPPMVQHYAALAFKPHAKLPDTLPDPKLDVDVQRVAATSAKFGIPGNARL
EECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
IAFCPGAEYGPAKRWPAEHFAELAQMLRRSFPYAQIVALGSGKDREMANAIVERAPFVRN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCHHHHH
LCGETSLDEAIELLARAEAAICNDSGLMHVTAALGRPQVAVFGSSDPRHTPPLSPAASIM
HCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEE
WLHLECSPCFARECPLGHLRCLRDIGPEMVFHELRRLLQPQ
EEEEECCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRKILVVAPNWIGDALMAQPLFAQLKARHPRAQIHAIAPKWVAPVLARMPEIARVLPTE
CCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHH
LAHGKLQLGSRTMFAQQLKGETFDAAYVLPNSFKSALIPWLAGIPLRIGYKGESRLGVLN
HCCCEEECCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCEEEECCCCCCEEEEE
VRYPNPPKNARPPMVQHYAALAFKPHAKLPDTLPDPKLDVDVQRVAATSAKFGIPGNARL
EECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEE
IAFCPGAEYGPAKRWPAEHFAELAQMLRRSFPYAQIVALGSGKDREMANAIVERAPFVRN
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCHHHHH
LCGETSLDEAIELLARAEAAICNDSGLMHVTAALGRPQVAVFGSSDPRHTPPLSPAASIM
HCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEE
WLHLECSPCFARECPLGHLRCLRDIGPEMVFHELRRLLQPQ
EEEEECCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]