| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is hsdM [C]
Identifier: 187927550
GI number: 187927550
Start: 483006
End: 484619
Strand: Direct
Name: hsdM [C]
Synonym: Rpic_0450
Alternate gene names: 187927550
Gene position: 483006-484619 (Clockwise)
Preceding gene: 187927549
Following gene: 187927551
Centisome position: 12.25
GC content: 57.31
Gene sequence:
>1614_bases ATGACCGATCTCGAAAAACAAAAGCTGGGCAAGACCCTCTGGGCCATTGCAGACCAACTGCGCGGCGCAATGAATGCGGA CGATTTCCGCGACTACATGCTGGCTTTCCTGTTCCTGCGCTACCTGTCCGACAATTACGAGGCCGCCGCCAAGAAGGAGT TGGGGCGGGACTACCCGCAGCAGCTCGACAGCTCGGTGTCCACCCCTCTGCAACGCTGGTATGAGAGCAACCTGGACGAC GTGCCAGAGTTCGAGAAGCAAATGCGCCGCAAGGTGCACTACGTGATCGAGCCGCAATTCCTGTGGGGCAACATTGCCGA GATGGCGAGGACACAAGATGCAGTGCTACTGAAAACCTTGCAGAAAGGCTTCAGCTACATCGAGACCGAATCCTTCGCCA GCACCTTCCGAGGGCTGTTCTCGGAGATCAACCTGGCATCCGACAAGCTCGGCAAAACCTATGCTGAGCGCAATGCCCGC CTGTGCAAGATCATCAAGGAAATTGCAGACGGACTGAAGCAGTTTTCCACCGACAGCGACACGTTGGGTGACGCCTACGA GTACTTGATTGGTCAGTTTGCGGCTGGCTCGGGCAAGAAGGCGGGCGAGTTCTACACCCCTCAGCGCATTTCGGACATCT TGTCGGCCATCGTGACGCTCGATAGCCAAGAGCCCGCTACGGGTAAGCGCTCCCATCTCGACAGAGTGATGGATCTGGCC TGCGGCTCAGGTTCCCTGTTATTGAACGTGCGCCACCGCATGAAGGAAGCAAAGGGCACGATCGGGAAAATTTACGGGCA GGAAAAGAACATTACCACCTACAACCTGGCGCGCATGAACATGCTGCTGCACGGGGTGAAGGATTCGGAGTTTGAGATTT TCCATGGCGACACCCTGCTCAACGAGTGGGACATGTTGCGTGAGACCAATCCGGCCAAGATGCCGAAGTTTGACGCGGTG GTGGCCAACCCTCCGTTCAGCTACCGGTGGGAGCCCACCGAAGCACTGGGCGAGGACGTGCGGTTCAAGAATTACGGCCT AGCACCCAAGTCCGCTGCCGACTTTGCCTTCCTGCTGCACGGCTTCCAATTCCTGAAGCAAGACGGGGTGATGGCCATCA TCCTGCCGCATGGTGTGCTATTCCGAGGTGGTGCGGAGGCACGCATCCGCACCAAGCTACTCAAGGACGGCCACATCGAC ACGGTGATCGGCCTGCCGGCCAACCTGTTCTTTTCCACGGGCATCCCGGTATGCATCTTGGTGCTGAAGAAGTGCAAGAA GCCGGATGATGTGCTGTTCATCAACGCAGCCGAGCACTTCGAGAAGGGGAAGCGGCAGAACCAGATCCTGCCCGAGCACA TCGACAAGATCATCGACACCTATCAGTTCCGCAAGGAGGAGGCACGCTATTCGCGGCGCGTGGGCATGGAAGAAATCGAG AAGAACGATTTCAATCTGAACATCTCGCGCTACGTGAGCACGACGGAAGCCGAGGAGGAGATTGATCTCGCGGCGGTGCA CGCTGAACTGGTTTCACTGGACAAGAAGATCAAGATGGCTACCGAGCAGCACAACAAATTCCTCAAAGAGCTAGGGCTGC CGCTGCTGCCGTGA
Upstream 100 bases:
>100_bases AGAACAAGGCTTACTTCAGGAAGATCCTGCACGACTTCCTGAATCGCTATCCCTTCAATCCAGATCTATTTCCTCAAGCC GTTGAGGAAGCAGGCACCCA
Downstream 100 bases:
>100_bases TGAGCGCGGCCAAGACCAGACCGGTCTTGGCTTTCAGCCGAGATGAGGAGAGGCAAGCTGCGCGCGCTTGCCTCTATCTT GAGACGTACCCACTCGGTGC
Product: type I restriction-modification system, M subunit
Products: NA
Alternate protein names: M.MpnORFDP [H]
Number of amino acids: Translated: 537; Mature: 536
Protein sequence:
>537_residues MTDLEKQKLGKTLWAIADQLRGAMNADDFRDYMLAFLFLRYLSDNYEAAAKKELGRDYPQQLDSSVSTPLQRWYESNLDD VPEFEKQMRRKVHYVIEPQFLWGNIAEMARTQDAVLLKTLQKGFSYIETESFASTFRGLFSEINLASDKLGKTYAERNAR LCKIIKEIADGLKQFSTDSDTLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIVTLDSQEPATGKRSHLDRVMDLA CGSGSLLLNVRHRMKEAKGTIGKIYGQEKNITTYNLARMNMLLHGVKDSEFEIFHGDTLLNEWDMLRETNPAKMPKFDAV VANPPFSYRWEPTEALGEDVRFKNYGLAPKSAADFAFLLHGFQFLKQDGVMAIILPHGVLFRGGAEARIRTKLLKDGHID TVIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINAAEHFEKGKRQNQILPEHIDKIIDTYQFRKEEARYSRRVGMEEIE KNDFNLNISRYVSTTEAEEEIDLAAVHAELVSLDKKIKMATEQHNKFLKELGLPLLP
Sequences:
>Translated_537_residues MTDLEKQKLGKTLWAIADQLRGAMNADDFRDYMLAFLFLRYLSDNYEAAAKKELGRDYPQQLDSSVSTPLQRWYESNLDD VPEFEKQMRRKVHYVIEPQFLWGNIAEMARTQDAVLLKTLQKGFSYIETESFASTFRGLFSEINLASDKLGKTYAERNAR LCKIIKEIADGLKQFSTDSDTLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIVTLDSQEPATGKRSHLDRVMDLA CGSGSLLLNVRHRMKEAKGTIGKIYGQEKNITTYNLARMNMLLHGVKDSEFEIFHGDTLLNEWDMLRETNPAKMPKFDAV VANPPFSYRWEPTEALGEDVRFKNYGLAPKSAADFAFLLHGFQFLKQDGVMAIILPHGVLFRGGAEARIRTKLLKDGHID TVIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINAAEHFEKGKRQNQILPEHIDKIIDTYQFRKEEARYSRRVGMEEIE KNDFNLNISRYVSTTEAEEEIDLAAVHAELVSLDKKIKMATEQHNKFLKELGLPLLP >Mature_536_residues TDLEKQKLGKTLWAIADQLRGAMNADDFRDYMLAFLFLRYLSDNYEAAAKKELGRDYPQQLDSSVSTPLQRWYESNLDDV PEFEKQMRRKVHYVIEPQFLWGNIAEMARTQDAVLLKTLQKGFSYIETESFASTFRGLFSEINLASDKLGKTYAERNARL CKIIKEIADGLKQFSTDSDTLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIVTLDSQEPATGKRSHLDRVMDLAC GSGSLLLNVRHRMKEAKGTIGKIYGQEKNITTYNLARMNMLLHGVKDSEFEIFHGDTLLNEWDMLRETNPAKMPKFDAVV ANPPFSYRWEPTEALGEDVRFKNYGLAPKSAADFAFLLHGFQFLKQDGVMAIILPHGVLFRGGAEARIRTKLLKDGHIDT VIGLPANLFFSTGIPVCILVLKKCKKPDDVLFINAAEHFEKGKRQNQILPEHIDKIIDTYQFRKEEARYSRRVGMEEIEK NDFNLNISRYVSTTEAEEEIDLAAVHAELVSLDKKIKMATEQHNKFLKELGLPLLP
Specific function: Methylation of specific adenine residues; required for both restriction and modification activities [H]
COG id: COG0286
COG function: function code V; Type I restriction-modification system methyltransferase subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
Organism=Escherichia coli, GI1790808, Length=261, Percent_Identity=25.6704980842912, Blast_Score=83, Evalue=5e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022749 - InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 - InterPro: IPR004546 [H]
Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 61042; Mature: 60910
Theoretical pI: Translated: 6.66; Mature: 6.66
Prosite motif: PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTDLEKQKLGKTLWAIADQLRGAMNADDFRDYMLAFLFLRYLSDNYEAAAKKELGRDYPQ CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH QLDSSVSTPLQRWYESNLDDVPEFEKQMRRKVHYVIEPQFLWGNIAEMARTQDAVLLKTL HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH QKGFSYIETESFASTFRGLFSEINLASDKLGKTYAERNARLCKIIKEIADGLKQFSTDSD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC TLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIVTLDSQEPATGKRSHLDRVMDLA HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH CGSGSLLLNVRHRMKEAKGTIGKIYGQEKNITTYNLARMNMLLHGVKDSEFEIFHGDTLL CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHH NEWDMLRETNPAKMPKFDAVVANPPFSYRWEPTEALGEDVRFKNYGLAPKSAADFAFLLH HHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHH GFQFLKQDGVMAIILPHGVLFRGGAEARIRTKLLKDGHIDTVIGLPANLFFSTGIPVCIL HHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHCCHHHHHH VLKKCKKPDDVLFINAAEHFEKGKRQNQILPEHIDKIIDTYQFRKEEARYSRRVGMEEIE HHHHCCCCCCEEEEECHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH KNDFNLNISRYVSTTEAEEEIDLAAVHAELVSLDKKIKMATEQHNKFLKELGLPLLP CCCCCEEHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC >Mature Secondary Structure TDLEKQKLGKTLWAIADQLRGAMNADDFRDYMLAFLFLRYLSDNYEAAAKKELGRDYPQ CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHH QLDSSVSTPLQRWYESNLDDVPEFEKQMRRKVHYVIEPQFLWGNIAEMARTQDAVLLKTL HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH QKGFSYIETESFASTFRGLFSEINLASDKLGKTYAERNARLCKIIKEIADGLKQFSTDSD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC TLGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIVTLDSQEPATGKRSHLDRVMDLA HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH CGSGSLLLNVRHRMKEAKGTIGKIYGQEKNITTYNLARMNMLLHGVKDSEFEIFHGDTLL CCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCHHH NEWDMLRETNPAKMPKFDAVVANPPFSYRWEPTEALGEDVRFKNYGLAPKSAADFAFLLH HHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHH GFQFLKQDGVMAIILPHGVLFRGGAEARIRTKLLKDGHIDTVIGLPANLFFSTGIPVCIL HHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHCCHHHHHH VLKKCKKPDDVLFINAAEHFEKGKRQNQILPEHIDKIIDTYQFRKEEARYSRRVGMEEIE HHHHCCCCCCEEEEECHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH KNDFNLNISRYVSTTEAEEEIDLAAVHAELVSLDKKIKMATEQHNKFLKELGLPLLP CCCCCEEHHHHCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8948633 [H]