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The map label for this gene is glyS

Identifier: 187927503

GI number: 187927503

Start: 424687

End: 426780

Strand: Reverse

Name: glyS

Synonym: Rpic_0400

Alternate gene names: 187927503

Gene position: 426780-424687 (Counterclockwise)

Preceding gene: 187927504

Following gene: 187927502

Centisome position: 10.82

GC content: 66.48

Gene sequence:

>2094_bases
ATGTCCACCCTCCTGATCGAACTGCTGACCGAAGAACTGCCGCCCAAGGCGCTGGCCCGCCTGGGCGAGGCGTTTGCACG
CGGCCTGTTTGACGGTCTGTCGGCGCAAGGCCTGCTCGAAGAGGGCGCCGCCGTGGAAGGATTTGCCACGCCGCGCCGCC
TGGCCGCATCGATCACCGGCGCGCGCCGCACCGCGCCCGATCGCGAACTGCGCGAGAAGGTGCTGCCCGTGACCATCGCC
TTCGACGCCGAGGGCAAGCCCGCAGCGCCGCTGGTCAAGAAGCTGGCCGCGCTGGCCAAGACCGTGGGCGTGGAGGTCAT
CGCCGCTGAATCGCTGGAACGCGGGCCCGACGGCAAGGCCGAAGCGCTGTTTTATCGCTACACCGCACGCGGCGCCGTGC
TGGCTGACGGCCTTCAGACTGCACTGTCGCAAACCATCGCCAACCTTCCGATCCCGAAGGTGATGACGTATCAGCGCCCG
AACGGCGAGAACGTCCAGTTCGTGCGCCCCGCCCACAAGCTGATTGCGCTGCTCGACAGCGAGGTCATCCCCGTCAGCGC
GTTCGGCCTGCAATCGGGCAACGTGACGCTGGGCCACCGTTTCCTGTCGGCGGGCGAAATCGTCATCCAGGACGCCGCGT
CGTATGCGAGCACGCTGGAGTCGCAGGGCAAGGTCATCGCCGGCTACGGCAAGCGCAAAGAGGCCATCCGCGCCGAACTG
CTCAAGACCGCCGGCGCCGATACGGTAGTCATGCCCGAAGCGCTACTCGATGAGGTGAACGCGTTGGTCGAATGGCCCGT
GGTCTACCCCTGCCATTTCGAGGAAGCGTTCCTCGCCGTACCGCAGGAGTGTCTGATCCTGACCATGCAGACCAACCAGA
AGTACTTTGCGCTGACCGACGCGCAGGGCCATCTGCGCAACCGCTTCCTGATCGTCTCGAACATCGCCACGGATACACCC
GAGGCCATCATTCAAGGCAATGAGCGCGTGGTCCGCCCGCGCCTGGCCGACGCGCGCTTCTTCTTCGAGCAGGACAAGAA
GAAGCCGCTGGCCGATCGCGTGCCGCTGCTCTCGCGCGTGGTCTATCACAACAAGATCGGCACGCAACTTGAGCGCGTGT
CGCGCCTGCAAGCCATTGCCGGCCAACTGGCCGAGAAGCTCGGCGCGGACGTGGCACACGCCTCGCGCGGCGCGCTGCTG
GCCAAAGCCGATCTGCTGACCGACATGGTGGGCGAGTTCCCCGAGCTGCAAGGCACGATGGGGACGTACTACGCCCGACA
CGACGGCGAACCCGACGACGTGGCGCTGGCCTGCTCCGAACACTACCAACCGCGCTTTGCCGGCGATGCGCTGCCCAGCA
CCGCCACCGGTACCGTGGTGGCGCTGGCCGACAAGCTCGAAACGCTGGTCGGCATCTGGGGCATCGGCCTGCAACCCACG
GGCGAGAAAGACCCGTTCGCGCTGCGCCGCCACGCGCTGGGCATCCTGCGCATGCTGATCGAGAAGCCGCTCGCGCTCAC
GATTGACGATGCACTGCAGATCGCCGCTGCCAGCTTCGACGGCATCGCCGCCGTCAAACCGAACCTGGCCGCCATCACCG
ACTTCCTCTACGACCGCCTGCGCGGCTACCTGAAGGACAAGGGCTACAGCACCAACGAGGTCGAAGCCGTGGTGAGTCAG
CGCCCGCAGCGCCTAGACGACATCGTCGCGCGCCTAGAAGCCGTACGCGCCTTTGCTGCCCTGCCGCAGGCCGAGGCGCT
GGCGGCCGCCAACAAGCGCATCACCAACATCCTGAAGAAAACGGACGTGGCCATTGGTGCCGTGCAGCCGCCACTGCTCA
AGGAAGACGCCGAGCGCGCGCTGCACCAATCCGTGGTTTCAGCGGAACCGCAGGTGCACGACGCCTTCGCACGCGGCGAT
TTCACCACTGCGCTCAAGACGTTGGCCGGTCTGCGCGAAGCGGTCGACACCTTCTTCGACGGCGTGATGGTGATGGCCGA
CGACACCGCGCTGCGCGACAACCGCCTGGCGCTGCTGGCTGAACTGCACGGACTGATGAACCGCGTGGCGGACATCTCCA
AGCTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCTCGCCCTGCTGATCACTGAACCGAACCACACAACGTACCGCCGCACCTGATCTGAACACCAAGCGGCGCAGAACAACG
ACGTCACCGATTCAAACGCT

Downstream 100 bases:

>100_bases
TCGGCACCCCAAGCACCGTTCGGAGCCCACCATGCCCCACGTTCCCAAACTGGTGATTCTCGACCGCGATGGCGTCATCA
ACCGAGACAGCGACCAGTTC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSTLLIELLTEELPPKALARLGEAFARGLFDGLSAQGLLEEGAAVEGFATPRRLAASITGARRTAPDRELREKVLPVTIA
FDAEGKPAAPLVKKLAALAKTVGVEVIAAESLERGPDGKAEALFYRYTARGAVLADGLQTALSQTIANLPIPKVMTYQRP
NGENVQFVRPAHKLIALLDSEVIPVSAFGLQSGNVTLGHRFLSAGEIVIQDAASYASTLESQGKVIAGYGKRKEAIRAEL
LKTAGADTVVMPEALLDEVNALVEWPVVYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNIATDTP
EAIIQGNERVVRPRLADARFFFEQDKKKPLADRVPLLSRVVYHNKIGTQLERVSRLQAIAGQLAEKLGADVAHASRGALL
AKADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACSEHYQPRFAGDALPSTATGTVVALADKLETLVGIWGIGLQPT
GEKDPFALRRHALGILRMLIEKPLALTIDDALQIAAASFDGIAAVKPNLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQ
RPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDVAIGAVQPPLLKEDAERALHQSVVSAEPQVHDAFARGD
FTTALKTLAGLREAVDTFFDGVMVMADDTALRDNRLALLAELHGLMNRVADISKLAA

Sequences:

>Translated_697_residues
MSTLLIELLTEELPPKALARLGEAFARGLFDGLSAQGLLEEGAAVEGFATPRRLAASITGARRTAPDRELREKVLPVTIA
FDAEGKPAAPLVKKLAALAKTVGVEVIAAESLERGPDGKAEALFYRYTARGAVLADGLQTALSQTIANLPIPKVMTYQRP
NGENVQFVRPAHKLIALLDSEVIPVSAFGLQSGNVTLGHRFLSAGEIVIQDAASYASTLESQGKVIAGYGKRKEAIRAEL
LKTAGADTVVMPEALLDEVNALVEWPVVYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNIATDTP
EAIIQGNERVVRPRLADARFFFEQDKKKPLADRVPLLSRVVYHNKIGTQLERVSRLQAIAGQLAEKLGADVAHASRGALL
AKADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACSEHYQPRFAGDALPSTATGTVVALADKLETLVGIWGIGLQPT
GEKDPFALRRHALGILRMLIEKPLALTIDDALQIAAASFDGIAAVKPNLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQ
RPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDVAIGAVQPPLLKEDAERALHQSVVSAEPQVHDAFARGD
FTTALKTLAGLREAVDTFFDGVMVMADDTALRDNRLALLAELHGLMNRVADISKLAA
>Mature_696_residues
STLLIELLTEELPPKALARLGEAFARGLFDGLSAQGLLEEGAAVEGFATPRRLAASITGARRTAPDRELREKVLPVTIAF
DAEGKPAAPLVKKLAALAKTVGVEVIAAESLERGPDGKAEALFYRYTARGAVLADGLQTALSQTIANLPIPKVMTYQRPN
GENVQFVRPAHKLIALLDSEVIPVSAFGLQSGNVTLGHRFLSAGEIVIQDAASYASTLESQGKVIAGYGKRKEAIRAELL
KTAGADTVVMPEALLDEVNALVEWPVVYPCHFEEAFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNIATDTPE
AIIQGNERVVRPRLADARFFFEQDKKKPLADRVPLLSRVVYHNKIGTQLERVSRLQAIAGQLAEKLGADVAHASRGALLA
KADLLTDMVGEFPELQGTMGTYYARHDGEPDDVALACSEHYQPRFAGDALPSTATGTVVALADKLETLVGIWGIGLQPTG
EKDPFALRRHALGILRMLIEKPLALTIDDALQIAAASFDGIAAVKPNLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQR
PQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDVAIGAVQPPLLKEDAERALHQSVVSAEPQVHDAFARGDF
TTALKTLAGLREAVDTFFDGVMVMADDTALRDNRLALLAELHGLMNRVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=701, Percent_Identity=45.9343794579173, Blast_Score=555, Evalue=1e-159,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_RALPJ (B2UFN5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897990.1
- ProteinModelPortal:   B2UFN5
- GeneID:   6288503
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0400
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75194; Mature: 75063

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLLIELLTEELPPKALARLGEAFARGLFDGLSAQGLLEEGAAVEGFATPRRLAASITG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHCC
ARRTAPDRELREKVLPVTIAFDAEGKPAAPLVKKLAALAKTVGVEVIAAESLERGPDGKA
CCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCC
EALFYRYTARGAVLADGLQTALSQTIANLPIPKVMTYQRPNGENVQFVRPAHKLIALLDS
CEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEECHHHHHHHHHCC
EVIPVSAFGLQSGNVTLGHRFLSAGEIVIQDAASYASTLESQGKVIAGYGKRKEAIRAEL
CCCEEEEECCCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHH
LKTAGADTVVMPEALLDEVNALVEWPVVYPCHFEEAFLAVPQECLILTMQTNQKYFALTD
HHHCCCCCEECCHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCEEEEEECCCEEEEEEC
AQGHLRNRFLIVSNIATDTPEAIIQGNERVVRPRLADARFFFEQDKKKPLADRVPLLSRV
CCHHHCCCEEEEECCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VYHNKIGTQLERVSRLQAIAGQLAEKLGADVAHASRGALLAKADLLTDMVGEFPELQGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCH
GTYYARHDGEPDDVALACSEHYQPRFAGDALPSTATGTVVALADKLETLVGIWGIGLQPT
HHEEECCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GEKDPFALRRHALGILRMLIEKPLALTIDDALQIAAASFDGIAAVKPNLAAITDFLYDRL
CCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHH
RGYLKDKGYSTNEVEAVVSQRPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKK
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TDVAIGAVQPPLLKEDAERALHQSVVSAEPQVHDAFARGDFTTALKTLAGLREAVDTFFD
HCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GVMVMADDTALRDNRLALLAELHGLMNRVADISKLAA
CEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLLIELLTEELPPKALARLGEAFARGLFDGLSAQGLLEEGAAVEGFATPRRLAASITG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHCC
ARRTAPDRELREKVLPVTIAFDAEGKPAAPLVKKLAALAKTVGVEVIAAESLERGPDGKA
CCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCC
EALFYRYTARGAVLADGLQTALSQTIANLPIPKVMTYQRPNGENVQFVRPAHKLIALLDS
CEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEECHHHHHHHHHCC
EVIPVSAFGLQSGNVTLGHRFLSAGEIVIQDAASYASTLESQGKVIAGYGKRKEAIRAEL
CCCEEEEECCCCCCEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHH
LKTAGADTVVMPEALLDEVNALVEWPVVYPCHFEEAFLAVPQECLILTMQTNQKYFALTD
HHHCCCCCEECCHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCEEEEEECCCEEEEEEC
AQGHLRNRFLIVSNIATDTPEAIIQGNERVVRPRLADARFFFEQDKKKPLADRVPLLSRV
CCHHHCCCEEEEECCCCCCCHHHHCCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHH
VYHNKIGTQLERVSRLQAIAGQLAEKLGADVAHASRGALLAKADLLTDMVGEFPELQGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCH
GTYYARHDGEPDDVALACSEHYQPRFAGDALPSTATGTVVALADKLETLVGIWGIGLQPT
HHEEECCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GEKDPFALRRHALGILRMLIEKPLALTIDDALQIAAASFDGIAAVKPNLAAITDFLYDRL
CCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHH
RGYLKDKGYSTNEVEAVVSQRPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKK
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TDVAIGAVQPPLLKEDAERALHQSVVSAEPQVHDAFARGDFTTALKTLAGLREAVDTFFD
HCCHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GVMVMADDTALRDNRLALLAELHGLMNRVADISKLAA
CEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA