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The map label for this gene is paaF [C]

Identifier: 187927455

GI number: 187927455

Start: 376560

End: 377324

Strand: Direct

Name: paaF [C]

Synonym: Rpic_0352

Alternate gene names: 187927455

Gene position: 376560-377324 (Clockwise)

Preceding gene: 187927454

Following gene: 187927457

Centisome position: 9.55

GC content: 65.36

Gene sequence:

>765_bases
ATGTCGATTCGTACCGAGACCGCAGACGGCGTCCTGACGCTGACCTTTGATCGTCTGGACCGCAAGAACGCCATCACGGC
CGCGATGTATCAAACGTTGGCCGATGCCCTGGTGGCTGCCGAAACCGATCCCGCCATCCGCGTGATTGTGCTGGCCGGCC
ACGAGAGCGTGTTCACGGCGGGCAACGATCTCGAAGACTTCATGAAGAATCCGCCCAAGGACGAGAGCGCCCCTGTGCAC
CAGTTCCTGCGAGCGATCAGCACAGCGAGCAAGCCGTTGATCGCCTCCGTGAGCGGCGCGGCTGTCGGCGTGGGTACGAC
CATGCTGCTGCATTGCGACCTCGTCTACGCATCCGAGACGGCCAAGCTGTCGATGCCATTCGCGCAGCTGGGCCTCTGCC
CTGAAGCCGCATCGAGCTTGCTGCTGCCGCAGCTGGCCGGCTACCACCGCGCGGCGGACAAGCTGCTCTTCGGCGAGCCG
TTCGACGCGAACGAAGCGCGCGAGCTGGGGCTGGTCAATCGCGTGCTGCCGGCTGGCGAGCTGGATGCCTTCGTGCGTAC
GCAGGCGCGCAAGCTGACGCTGCTGCCGCCCGCATCGCTGCGCGCCACCAAGCGCCTGATGAAGGAGGGCGCCACGCCGC
AGATCGCCGAGCGCATGTCGGTGGAAGGCAAGCAATTTGGGGAAATGCTGCGTGCCCCCGAGGCGCGCGAGGCGTTCACG
GCGTTCTTCGAAAAGCGCCGCCCGGATTTCTCCAAGTTCAACTGA

Upstream 100 bases:

>100_bases
TGACCGTGGCGTTGAACGTGGGGGCCAACGCGTTCTCCGTCGGTTATCTATCTGATTCGCCTGTGCCGATTTAGCCGCGC
AGGCTTCACCTGGAGTTTTC

Downstream 100 bases:

>100_bases
GCGAATCGTTCACCTGCAAAAGAAAACCCGGCGCGAAGCCGGGTTTTTTCTTGGCCGTTGCCCCTTCAGAGCGGCGGCAG
CACGCGCGGCTTGCGAGTCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSIRTETADGVLTLTFDRLDRKNAITAAMYQTLADALVAAETDPAIRVIVLAGHESVFTAGNDLEDFMKNPPKDESAPVH
QFLRAISTASKPLIASVSGAAVGVGTTMLLHCDLVYASETAKLSMPFAQLGLCPEAASSLLLPQLAGYHRAADKLLFGEP
FDANEARELGLVNRVLPAGELDAFVRTQARKLTLLPPASLRATKRLMKEGATPQIAERMSVEGKQFGEMLRAPEAREAFT
AFFEKRRPDFSKFN

Sequences:

>Translated_254_residues
MSIRTETADGVLTLTFDRLDRKNAITAAMYQTLADALVAAETDPAIRVIVLAGHESVFTAGNDLEDFMKNPPKDESAPVH
QFLRAISTASKPLIASVSGAAVGVGTTMLLHCDLVYASETAKLSMPFAQLGLCPEAASSLLLPQLAGYHRAADKLLFGEP
FDANEARELGLVNRVLPAGELDAFVRTQARKLTLLPPASLRATKRLMKEGATPQIAERMSVEGKQFGEMLRAPEAREAFT
AFFEKRRPDFSKFN
>Mature_253_residues
SIRTETADGVLTLTFDRLDRKNAITAAMYQTLADALVAAETDPAIRVIVLAGHESVFTAGNDLEDFMKNPPKDESAPVHQ
FLRAISTASKPLIASVSGAAVGVGTTMLLHCDLVYASETAKLSMPFAQLGLCPEAASSLLLPQLAGYHRAADKLLFGEPF
DANEARELGLVNRVLPAGELDAFVRTQARKLTLLPPASLRATKRLMKEGATPQIAERMSVEGKQFGEMLRAPEAREAFTA
FFEKRRPDFSKFN

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=35.7142857142857, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=35.7142857142857, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI260274832, Length=210, Percent_Identity=35.7142857142857, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI194097323, Length=238, Percent_Identity=27.7310924369748, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=25.4166666666667, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=25.4166666666667, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI4757966, Length=198, Percent_Identity=28.7878787878788, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=198, Percent_Identity=28.7878787878788, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=240, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI49355787, Length=240, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=23.4042553191489, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=23.4042553191489, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=25.8964143426295, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221307494, Length=235, Percent_Identity=24.2553191489362, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=25.8555133079848, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI68989263, Length=168, Percent_Identity=30.3571428571429, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI116235487, Length=161, Percent_Identity=31.055900621118, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=26.3779527559055, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=33.469387755102, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=32.421875, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=28.735632183908, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI221142681, Length=245, Percent_Identity=29.7959183673469, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=26.2745098039216, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=25.6983240223464, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=31.4516129032258, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=34.703196347032, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=30, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=241, Percent_Identity=29.045643153527, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=26.1061946902655, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=182, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=25.5813953488372, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=248, Percent_Identity=27.0161290322581, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=170, Percent_Identity=27.0588235294118, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24654903, Length=248, Percent_Identity=30.6451612903226, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=29.0196078431373, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=29.0196078431373, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=26, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=28.1081081081081, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=27.3224043715847, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650670, Length=221, Percent_Identity=27.1493212669683, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27550; Mature: 27419

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRTETADGVLTLTFDRLDRKNAITAAMYQTLADALVAAETDPAIRVIVLAGHESVFTA
CCCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEC
GNDLEDFMKNPPKDESAPVHQFLRAISTASKPLIASVSGAAVGVGTTMLLHCDLVYASET
CCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHEEEHHHHHHEECCC
AKLSMPFAQLGLCPEAASSLLLPQLAGYHRAADKLLFGEPFDANEARELGLVNRVLPAGE
CEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCH
LDAFVRTQARKLTLLPPASLRATKRLMKEGATPQIAERMSVEGKQFGEMLRAPEAREAFT
HHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHH
AFFEKRRPDFSKFN
HHHHHCCCCCCCCC
>Mature Secondary Structure 
SIRTETADGVLTLTFDRLDRKNAITAAMYQTLADALVAAETDPAIRVIVLAGHESVFTA
CCCCCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEC
GNDLEDFMKNPPKDESAPVHQFLRAISTASKPLIASVSGAAVGVGTTMLLHCDLVYASET
CCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHEEEHHHHHHEECCC
AKLSMPFAQLGLCPEAASSLLLPQLAGYHRAADKLLFGEPFDANEARELGLVNRVLPAGE
CEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCH
LDAFVRTQARKLTLLPPASLRATKRLMKEGATPQIAERMSVEGKQFGEMLRAPEAREAFT
HHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHH
AFFEKRRPDFSKFN
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA