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The map label for this gene is queF [H]

Identifier: 187927427

GI number: 187927427

Start: 341017

End: 341853

Strand: Reverse

Name: queF [H]

Synonym: Rpic_0324

Alternate gene names: 187927427

Gene position: 341853-341017 (Counterclockwise)

Preceding gene: 187927428

Following gene: 187927426

Centisome position: 8.67

GC content: 62.01

Gene sequence:

>837_bases
ATGAGCAACCAACCCGAACATTCGCCGCTGGGCAAGACGTCCGCCTACAAGACGGAGTACGACCCGCACCTGCTGTTTCC
GATTCCGCGCCAGGGCAAGCGCGACGAGATCGGCCTCGCCGCCGGCACGCCGCTGCCCTTCTTCGGCGTGGACCTCTGGA
ACCTGTACGAGCTGTCGTGGCTGAACCTGCGCGGCAAGCCGCAGGTGGCGCTGGGCACCGTGATCGTGCCGGCGGATTCG
CCCAACATCGTTGAATCGAAGTCGTTCAAGCTGTATCTGAATTCGTTCAACCAGACGAAGGTGGCGTCGCACGAGGCGCT
GCAGCAGTTGATCCACCACGATCTGTCGGAAGCATGCGGCGCGCCGGTGCAGGTGCGCATCGTCACGCAGGAAGAGTTCG
CGCGGCAGAAGATGGGCGAACTCGATGGCCTGCTGCTCGACCGCCTGGACATCGAAACAGATGTCTACCAGCCCACGCCC
GAGCTGCTACATGCCGATGAGGAAGAAAGCCCGGTGGAAGAAACGCTGGTGTCGCACCTGCTGAAGTCCAACTGCCTGGT
GACGGGCCAGCCGGACTGGGGCAGCGTGCAGATCCGCTACGTGGGCGCGCCGATCAACCAGGAAGCGCTGCTGAAGTATC
TGATCTCGTTCCGCGAACACAATGAATTTCACGAGCAGTGTGTGGAGCGGATCTTTACCGACATCCTGCGCCAGTGCCGC
CCGGTCAAGCTGGCCGTGTATGCGCGCTACACACGTCGCGGCGGGCTGGACATCAATCCGTTCCGCACAAACTACAACAC
ACCGTGGCCGGACAACTTGCGCAATGCACGTCAGTAA

Upstream 100 bases:

>100_bases
TACCGAAGAACGAGAAGCGCGAGTTCAAGGCGTTCCTCGCCACGCTGGGCTATACCTATTGGGACGAAAGCGAGAACCCC
GTCTACAGCCTGTTTTTGTG

Downstream 100 bases:

>100_bases
GCGCTGACGTTGGGCAAAACACGCAAGGCGCCTTCGGGCGCCTTTTTTCTTGCCCGAGCGATCCACCAAACGGGCATTTC
GGCCTCGTCCGTTTTGTGGA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSNQPEHSPLGKTSAYKTEYDPHLLFPIPRQGKRDEIGLAAGTPLPFFGVDLWNLYELSWLNLRGKPQVALGTVIVPADS
PNIVESKSFKLYLNSFNQTKVASHEALQQLIHHDLSEACGAPVQVRIVTQEEFARQKMGELDGLLLDRLDIETDVYQPTP
ELLHADEEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEALLKYLISFREHNEFHEQCVERIFTDILRQCR
PVKLAVYARYTRRGGLDINPFRTNYNTPWPDNLRNARQ

Sequences:

>Translated_278_residues
MSNQPEHSPLGKTSAYKTEYDPHLLFPIPRQGKRDEIGLAAGTPLPFFGVDLWNLYELSWLNLRGKPQVALGTVIVPADS
PNIVESKSFKLYLNSFNQTKVASHEALQQLIHHDLSEACGAPVQVRIVTQEEFARQKMGELDGLLLDRLDIETDVYQPTP
ELLHADEEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEALLKYLISFREHNEFHEQCVERIFTDILRQCR
PVKLAVYARYTRRGGLDINPFRTNYNTPWPDNLRNARQ
>Mature_277_residues
SNQPEHSPLGKTSAYKTEYDPHLLFPIPRQGKRDEIGLAAGTPLPFFGVDLWNLYELSWLNLRGKPQVALGTVIVPADSP
NIVESKSFKLYLNSFNQTKVASHEALQQLIHHDLSEACGAPVQVRIVTQEEFARQKMGELDGLLLDRLDIETDVYQPTPE
LLHADEEESPVEETLVSHLLKSNCLVTGQPDWGSVQIRYVGAPINQEALLKYLISFREHNEFHEQCVERIFTDILRQCRP
VKLAVYARYTRRGGLDINPFRTNYNTPWPDNLRNARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=54.4444444444444, Blast_Score=286, Evalue=7e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 31700; Mature: 31569

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQPEHSPLGKTSAYKTEYDPHLLFPIPRQGKRDEIGLAAGTPLPFFGVDLWNLYELSW
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCHHHHEEEEE
LNLRGKPQVALGTVIVPADSPNIVESKSFKLYLNSFNQTKVASHEALQQLIHHDLSEACG
ECCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
APVQVRIVTQEEFARQKMGELDGLLLDRLDIETDVYQPTPELLHADEEESPVEETLVSHL
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHH
LKSNCLVTGQPDWGSVQIRYVGAPINQEALLKYLISFREHNEFHEQCVERIFTDILRQCR
HHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
PVKLAVYARYTRRGGLDINPFRTNYNTPWPDNLRNARQ
CCEEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SNQPEHSPLGKTSAYKTEYDPHLLFPIPRQGKRDEIGLAAGTPLPFFGVDLWNLYELSW
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCEECCCHHHHEEEEE
LNLRGKPQVALGTVIVPADSPNIVESKSFKLYLNSFNQTKVASHEALQQLIHHDLSEACG
ECCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
APVQVRIVTQEEFARQKMGELDGLLLDRLDIETDVYQPTPELLHADEEESPVEETLVSHL
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHH
LKSNCLVTGQPDWGSVQIRYVGAPINQEALLKYLISFREHNEFHEQCVERIFTDILRQCR
HHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
PVKLAVYARYTRRGGLDINPFRTNYNTPWPDNLRNARQ
CCEEEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]