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The map label for this gene is mutY [H]

Identifier: 187927363

GI number: 187927363

Start: 272019

End: 273167

Strand: Direct

Name: mutY [H]

Synonym: Rpic_0256

Alternate gene names: 187927363

Gene position: 272019-273167 (Clockwise)

Preceding gene: 187927362

Following gene: 187927376

Centisome position: 6.9

GC content: 66.75

Gene sequence:

>1149_bases
ATGACCGCCAGCCCTCGCCGCACGCGCCGCGTTGCTCAGCCCGTTCCCGTATTGCCGCCGCTTCCTGACGATTTCGCCAC
GCGCGTGATCGCCTGGCAGCAGCGTCATGGCCGTCACCATCTGCCGTGGCAGAACACGGGCGATGCGTATCGCACATGGC
TCTCCGAGATCATGCTGCAGCAGACGCAAGTGAGCGCGGTGCTGGGCTACTACGCACGCTTCATCGAGCGCTTCCCGACT
GTGCAGGCGCTGGCCGCTGCGCCGGCAGACGACGTGATGGCAGCGTGGGCCGGTCTCGGCTACTACACGCGTGCGCGCAA
CCTGCATCGCTGCGCGCAAATCGTGGTGGCAGAACACGGCGGCATCTTCCCGCGCGATCCCGACGTGTTGGTCGCGCTGC
CCGGCATCGGCCGCTCGACCGCCGCGGCCATCGCCGCGTTCTCATACGGCGTGCGCGCGGCCATTCTCGATGGCAACGTC
AAGCGCGTGTTTGCACGTGCGTTCGGCATCGATGGCTTTCCCGGCGACAAACGCGTCGAAGACACCATGTGGCGCATCGC
CGAAACCGTGCTGCCGCCGGCCGAAGGCATTCAACCGTACACCCAGGGTTTGATGGACCTGGGAGCCACCGTCTGCACGC
GCGGCAAGCCGGCATGCCTGACCGGCGAACGCGCCTGTCCGCTCGAATCGCTATGCGAAGCGCGCAGCACCGGTCGCGTG
ATGGAGCTACCCGTGCCGCGCCCGCGCAAGGCGATACCCGAGCGCGCAGCGACACTCGTCATCGCGCTGCACGCCGACGC
GGTGTTGCTGCAGCGTCGTCCGCAACGCGGTATCTGGGGTGGGTTGTGGTCTTTGCCGCTGGTGGGCGAGATGGACGACG
CGCTGGACGCGCATCCGCTGAATGTCGACACCGTGCGTCGTGCCGCGCAGGCATATGGCGCGGTCTCAACCTTGGAGATG
GCCGGCGCGCTCACGCACACCTTCACACACTTCCGCCTGCAGATGCATCTGCTGCGCGCAGAGATTGCCTCGCCCGCTGC
GCTTGGCGACGACTGGCGCTGGATACCGCTTGCACAACTCAATTCAGTGGGATTGCCTGCGCCGGTGAAGTTGGCGCTGG
AGACGCTGGTGCAGCCAAGCCTGATCTAG

Upstream 100 bases:

>100_bases
GCATCGTTCGTTACGGAGAAGGCGCCTGCGGGCGCTTTTTTCGTTCTGGTAGGATCGCCGCCAGCGCGCACGTTCCGTCC
GCCGCTTTTCCATCACACGC

Downstream 100 bases:

>100_bases
CCAGCCCGGTCTGGCCAGGCGGATACGGCATCACAGAATGTGGTGTTGCTTCATGTAGCGATGGACGATCTCGATGCGCA
TGCCGGCGTCCATCAGTTCC

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTASPRRTRRVAQPVPVLPPLPDDFATRVIAWQQRHGRHHLPWQNTGDAYRTWLSEIMLQQTQVSAVLGYYARFIERFPT
VQALAAAPADDVMAAWAGLGYYTRARNLHRCAQIVVAEHGGIFPRDPDVLVALPGIGRSTAAAIAAFSYGVRAAILDGNV
KRVFARAFGIDGFPGDKRVEDTMWRIAETVLPPAEGIQPYTQGLMDLGATVCTRGKPACLTGERACPLESLCEARSTGRV
MELPVPRPRKAIPERAATLVIALHADAVLLQRRPQRGIWGGLWSLPLVGEMDDALDAHPLNVDTVRRAAQAYGAVSTLEM
AGALTHTFTHFRLQMHLLRAEIASPAALGDDWRWIPLAQLNSVGLPAPVKLALETLVQPSLI

Sequences:

>Translated_382_residues
MTASPRRTRRVAQPVPVLPPLPDDFATRVIAWQQRHGRHHLPWQNTGDAYRTWLSEIMLQQTQVSAVLGYYARFIERFPT
VQALAAAPADDVMAAWAGLGYYTRARNLHRCAQIVVAEHGGIFPRDPDVLVALPGIGRSTAAAIAAFSYGVRAAILDGNV
KRVFARAFGIDGFPGDKRVEDTMWRIAETVLPPAEGIQPYTQGLMDLGATVCTRGKPACLTGERACPLESLCEARSTGRV
MELPVPRPRKAIPERAATLVIALHADAVLLQRRPQRGIWGGLWSLPLVGEMDDALDAHPLNVDTVRRAAQAYGAVSTLEM
AGALTHTFTHFRLQMHLLRAEIASPAALGDDWRWIPLAQLNSVGLPAPVKLALETLVQPSLI
>Mature_381_residues
TASPRRTRRVAQPVPVLPPLPDDFATRVIAWQQRHGRHHLPWQNTGDAYRTWLSEIMLQQTQVSAVLGYYARFIERFPTV
QALAAAPADDVMAAWAGLGYYTRARNLHRCAQIVVAEHGGIFPRDPDVLVALPGIGRSTAAAIAAFSYGVRAAILDGNVK
RVFARAFGIDGFPGDKRVEDTMWRIAETVLPPAEGIQPYTQGLMDLGATVCTRGKPACLTGERACPLESLCEARSTGRVM
ELPVPRPRKAIPERAATLVIALHADAVLLQRRPQRGIWGGLWSLPLVGEMDDALDAHPLNVDTVRRAAQAYGAVSTLEMA
GALTHTFTHFRLQMHLLRAEIASPAALGDDWRWIPLAQLNSVGLPAPVKLALETLVQPSLI

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=405, Percent_Identity=33.8271604938272, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI6912520, Length=405, Percent_Identity=33.8271604938272, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI190358497, Length=405, Percent_Identity=33.8271604938272, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI115298654, Length=405, Percent_Identity=33.8271604938272, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI115298652, Length=405, Percent_Identity=33.8271604938272, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI115298650, Length=405, Percent_Identity=33.8271604938272, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789331, Length=357, Percent_Identity=43.1372549019608, Blast_Score=291, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 41748; Mature: 41617

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPRRTRRVAQPVPVLPPLPDDFATRVIAWQQRHGRHHLPWQNTGDAYRTWLSEIMLQ
CCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
QTQVSAVLGYYARFIERFPTVQALAAAPADDVMAAWAGLGYYTRARNLHRCAQIVVAEHG
HHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIFPRDPDVLVALPGIGRSTAAAIAAFSYGVRAAILDGNVKRVFARAFGIDGFPGDKRVE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCCCCHHH
DTMWRIAETVLPPAEGIQPYTQGLMDLGATVCTRGKPACLTGERACPLESLCEARSTGRV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHCCCE
MELPVPRPRKAIPERAATLVIALHADAVLLQRRPQRGIWGGLWSLPLVGEMDDALDAHPL
EECCCCCCHHHHHHHHHEEEEEECCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHCCCCCC
NVDTVRRAAQAYGAVSTLEMAGALTHTFTHFRLQMHLLRAEIASPAALGDDWRWIPLAQL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHH
NSVGLPAPVKLALETLVQPSLI
CCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TASPRRTRRVAQPVPVLPPLPDDFATRVIAWQQRHGRHHLPWQNTGDAYRTWLSEIMLQ
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
QTQVSAVLGYYARFIERFPTVQALAAAPADDVMAAWAGLGYYTRARNLHRCAQIVVAEHG
HHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GIFPRDPDVLVALPGIGRSTAAAIAAFSYGVRAAILDGNVKRVFARAFGIDGFPGDKRVE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCCCCHHH
DTMWRIAETVLPPAEGIQPYTQGLMDLGATVCTRGKPACLTGERACPLESLCEARSTGRV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHCCCE
MELPVPRPRKAIPERAATLVIALHADAVLLQRRPQRGIWGGLWSLPLVGEMDDALDAHPL
EECCCCCCHHHHHHHHHEEEEEECCCHHHHHCCCCCCCCCCHHHCCCCCCCHHHCCCCCC
NVDTVRRAAQAYGAVSTLEMAGALTHTFTHFRLQMHLLRAEIASPAALGDDWRWIPLAQL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHH
NSVGLPAPVKLALETLVQPSLI
CCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]