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The map label for this gene is rsmD [H]

Identifier: 187927351

GI number: 187927351

Start: 260154

End: 260810

Strand: Direct

Name: rsmD [H]

Synonym: Rpic_0244

Alternate gene names: 187927351

Gene position: 260154-260810 (Clockwise)

Preceding gene: 187927350

Following gene: 187927352

Centisome position: 6.6

GC content: 67.73

Gene sequence:

>657_bases
ATGGCACCTCGTACCCCCAGCTCCAAAGGCCCCAAGGCCAAGACCGCCACGTCGCATGCGCGCGCACCTCAGCAGGTGCG
CATCATCGGCGGGCAATACAAGCGCACGCCGCTCCCGGTGATCGACGCCGACGGCCTTCGCCCGACCAGCGACCGCGTCC
GCGAAACGGTCTTCAACTGGCTCGGTCATGACCTGACCGGCTGGCGCTGCGCCGACATCTTTGCCGGCACCGGGGCGCTC
GGCTTTGAGGCGGCTTCGCGCGGCGCCGCGCACGTGACGCTCGTGGAAAACCACGCCCAGGCTGTGCGCGCGTTGCACGC
CATCCGCGACAAGCTGGGCGCACGCATGGTCGACATCGTATCGGGCGACGCCTTTGCCTGGCTCGCTCGCCAGCCCGATG
CGGCGCTCGATGCGGTGTTCATCGATCCGCCGTTTGCACAGGACTGGACGTTACGCGCGTTGGAAGCCGCGGCCCGGGTG
GTCAAGCCCGGCGGCGTGATCTACGTTGAAGCGGCGCAGCAGCTGGCGGATGTGAGTGCTGATTCACATGGTGGCGAGCC
GGCCGCCGGCATCGGGCTACCGGCTGACCTGGTGCTCCACAAACACCTGCGCGCCGGTGCGGTGCATGCACATTTGCTGC
TCCGCAAAAACGGTTAA

Upstream 100 bases:

>100_bases
GAATTGACGCACGGGGTCTTTATCCGAGAAACCGGCGGCATTTCCCGCCGGTTTTTGTTTGCGCTCGTTTTATGGGGCAA
CAGCCCCTAAAATCGCGCCC

Downstream 100 bases:

>100_bases
ACTACGCCGCTCGCAGCCGATGCATGAGCATCGGGCGGCACCTGCAATGCGGGTGCGCTCGATCGATATCGGCGGCACAG
GCGGCAGGCTCGGGACCTGC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MAPRTPSSKGPKAKTATSHARAPQQVRIIGGQYKRTPLPVIDADGLRPTSDRVRETVFNWLGHDLTGWRCADIFAGTGAL
GFEAASRGAAHVTLVENHAQAVRALHAIRDKLGARMVDIVSGDAFAWLARQPDAALDAVFIDPPFAQDWTLRALEAAARV
VKPGGVIYVEAAQQLADVSADSHGGEPAAGIGLPADLVLHKHLRAGAVHAHLLLRKNG

Sequences:

>Translated_218_residues
MAPRTPSSKGPKAKTATSHARAPQQVRIIGGQYKRTPLPVIDADGLRPTSDRVRETVFNWLGHDLTGWRCADIFAGTGAL
GFEAASRGAAHVTLVENHAQAVRALHAIRDKLGARMVDIVSGDAFAWLARQPDAALDAVFIDPPFAQDWTLRALEAAARV
VKPGGVIYVEAAQQLADVSADSHGGEPAAGIGLPADLVLHKHLRAGAVHAHLLLRKNG
>Mature_217_residues
APRTPSSKGPKAKTATSHARAPQQVRIIGGQYKRTPLPVIDADGLRPTSDRVRETVFNWLGHDLTGWRCADIFAGTGALG
FEAASRGAAHVTLVENHAQAVRALHAIRDKLGARMVDIVSGDAFAWLARQPDAALDAVFIDPPFAQDWTLRALEAAARVV
KPGGVIYVEAAQQLADVSADSHGGEPAAGIGLPADLVLHKHLRAGAVHAHLLLRKNG

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=192, Percent_Identity=39.5833333333333, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23109; Mature: 22977

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRTPSSKGPKAKTATSHARAPQQVRIIGGQYKRTPLPVIDADGLRPTSDRVRETVFNW
CCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHH
LGHDLTGWRCADIFAGTGALGFEAASRGAAHVTLVENHAQAVRALHAIRDKLGARMVDIV
HCCCCCCCEEEHHHCCCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEE
SGDAFAWLARQPDAALDAVFIDPPFAQDWTLRALEAAARVVKPGGVIYVEAAQQLADVSA
CCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCC
DSHGGEPAAGIGLPADLVLHKHLRAGAVHAHLLLRKNG
CCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECC
>Mature Secondary Structure 
APRTPSSKGPKAKTATSHARAPQQVRIIGGQYKRTPLPVIDADGLRPTSDRVRETVFNW
CCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHHH
LGHDLTGWRCADIFAGTGALGFEAASRGAAHVTLVENHAQAVRALHAIRDKLGARMVDIV
HCCCCCCCEEEHHHCCCCCCCHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCCEEEEEE
SGDAFAWLARQPDAALDAVFIDPPFAQDWTLRALEAAARVVKPGGVIYVEAAQQLADVSA
CCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCC
DSHGGEPAAGIGLPADLVLHKHLRAGAVHAHLLLRKNG
CCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]