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The map label for this gene is rhtB [H]

Identifier: 187927301

GI number: 187927301

Start: 211603

End: 212244

Strand: Direct

Name: rhtB [H]

Synonym: Rpic_0193

Alternate gene names: 187927301

Gene position: 211603-212244 (Clockwise)

Preceding gene: 187927300

Following gene: 187927305

Centisome position: 5.37

GC content: 64.02

Gene sequence:

>642_bases
ATGACGTTGCAAGTCTGGCTGGCCTTCTTTTTGGCTGCCTGGGTGATCGCCATCTCGCCCGGGTCGGGCGCCATCCTGTG
CATGAGCCACGGCCTGACGCATGGCGTGCGCCGCACGTCGGTCACGATTGCCGGGCTGGAGCTGGGCATTGTCGTGATCC
TGTTCATAGCGGGTGCCGGGCTGGGCGCGCTGCTGATCGCGTCGGAGCACGCCTTTACGGCCATCAAGATCATTGGTGCG
ATGTACCTGATCTACCTGGGTATCGTCCAATGGCGCACGCCCATCCAGATCGCCAAGCCCACTGAGGGCGAGCCCCTGAA
GGTCTCGGGCAGCGGTGCGCGCCGTCGGTTCCTGGTTGGATTGCTGACCAATCTGACGAACCCGAAGGGCATCCTGTTCA
TGGTGGCCGTGCTGCCGCAGTTCATCGATCCGGCCAAGCCGCTGCTTTCGCAACTCGGCATCCTGGCGGCGACGATGGTG
TTCGTCGACCTGATCGTCATGCACGGCTATGCGCTGCTGGCCTCGCGCGCGCAGCGCCTGTTCCGCAATCCGCGCGCACT
GCGTTGGCAGAGCCGCTTCTTTGGCGGCATGCTGATGAGCATCGGCGCAGCGCTGTTCTTCGTGAAGCGCCAGACTGCGT
GA

Upstream 100 bases:

>100_bases
TCCGTACTAGGCCCGCACAGACCTGGGCGAACTGGCCGCAGCCGGTGCACAATTATCGTTTTGCGCTTTACCGCAACACA
ACGCATCACGGGGAACCATC

Downstream 100 bases:

>100_bases
GCTTCACACCGCCGCAGGGCTGCCCGCCAAGTCGTTGAGGGCACAGCGTGGGGATGCGCGCTAATCCGCCTTCACCCCGC
GCCGATACTGAATGGCCTCG

Product: Lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTLQVWLAFFLAAWVIAISPGSGAILCMSHGLTHGVRRTSVTIAGLELGIVVILFIAGAGLGALLIASEHAFTAIKIIGA
MYLIYLGIVQWRTPIQIAKPTEGEPLKVSGSGARRRFLVGLLTNLTNPKGILFMVAVLPQFIDPAKPLLSQLGILAATMV
FVDLIVMHGYALLASRAQRLFRNPRALRWQSRFFGGMLMSIGAALFFVKRQTA

Sequences:

>Translated_213_residues
MTLQVWLAFFLAAWVIAISPGSGAILCMSHGLTHGVRRTSVTIAGLELGIVVILFIAGAGLGALLIASEHAFTAIKIIGA
MYLIYLGIVQWRTPIQIAKPTEGEPLKVSGSGARRRFLVGLLTNLTNPKGILFMVAVLPQFIDPAKPLLSQLGILAATMV
FVDLIVMHGYALLASRAQRLFRNPRALRWQSRFFGGMLMSIGAALFFVKRQTA
>Mature_212_residues
TLQVWLAFFLAAWVIAISPGSGAILCMSHGLTHGVRRTSVTIAGLELGIVVILFIAGAGLGALLIASEHAFTAIKIIGAM
YLIYLGIVQWRTPIQIAKPTEGEPLKVSGSGARRRFLVGLLTNLTNPKGILFMVAVLPQFIDPAKPLLSQLGILAATMVF
VDLIVMHGYALLASRAQRLFRNPRALRWQSRFFGGMLMSIGAALFFVKRQTA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=207, Percent_Identity=42.0289855072464, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1788099, Length=166, Percent_Identity=28.3132530120482, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23029; Mature: 22898

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQVWLAFFLAAWVIAISPGSGAILCMSHGLTHGVRRTSVTIAGLELGIVVILFIAGAG
CHHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHCHHHHEEEEEHHHHHHHHHHHHHCCC
LGALLIASEHAFTAIKIIGAMYLIYLGIVQWRTPIQIAKPTEGEPLKVSGSGARRRFLVG
HHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHH
LLTNLTNPKGILFMVAVLPQFIDPAKPLLSQLGILAATMVFVDLIVMHGYALLASRAQRL
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNPRALRWQSRFFGGMLMSIGAALFFVKRQTA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLQVWLAFFLAAWVIAISPGSGAILCMSHGLTHGVRRTSVTIAGLELGIVVILFIAGAG
HHHHHHHHHHHHHHHEECCCCCEEEEEECCCHHCHHHHEEEEEHHHHHHHHHHHHHCCC
LGALLIASEHAFTAIKIIGAMYLIYLGIVQWRTPIQIAKPTEGEPLKVSGSGARRRFLVG
HHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCCHHHHHHHH
LLTNLTNPKGILFMVAVLPQFIDPAKPLLSQLGILAATMVFVDLIVMHGYALLASRAQRL
HHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNPRALRWQSRFFGGMLMSIGAALFFVKRQTA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]