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The map label for this gene is dctA1 [H]

Identifier: 187927294

GI number: 187927294

Start: 203997

End: 205286

Strand: Direct

Name: dctA1 [H]

Synonym: Rpic_0186

Alternate gene names: 187927294

Gene position: 203997-205286 (Clockwise)

Preceding gene: 187927289

Following gene: 187927295

Centisome position: 5.17

GC content: 61.24

Gene sequence:

>1290_bases
ATGAAAAAACCGTTCTACAAAATCCTGTACGTACAGGTGCTGGCGGCCATCGTCATCGGCGTTCTGCTGGGCCATTACTC
GCCCGAACTCGCCGTCAAGATGAAGCCGCTGGGCGATGGCTTCATCCAACTGATCAAGATGGTGATCGGCCCGATCATCT
TCTGTACTGTCGTCTCCGGCATTGCCGGCATGCGCGACATGAAGAAGGTGGGCCGCGTGGGCGGCAAGGCGTTGCTCTAC
TTCGAGGTCGTCTCGACCTTCGCGCTGATCATCGGTCTCGCGGCGGGCCACATCTTCAACCCCGGCGCGGGCTTTAACGT
CGACGTCAACACCATCGATGCCAAGGCTGTGGCGCAGTACGCCGCCAAGGCGCACTCGGCCAGCACGGTCGACTTCCTGC
TCAACATCATCCCGAGCACGGTGGTGGATGCCTTCGCCAAGGGCGACATCCTGCAGATCCTGCTGATCGCGCTGCTGTTT
GGGGGTGCACTTTCTGCCATGGGCGAGCGCGCGCAGATGGTGACGGACTTCATCGACCAGATCTCGCACGTGTTCTTCCG
TATCGTGCACGTCATCACGCGTGTGGCCCCGATCGGTGCCTTTGGCGCCATGGCTTTCACCATCGGCAAATACGGCGTGA
TCTCGCTGGTGCCGCTGCTCAAGCTGGTCGGCACGTTCTACTTCACGGCGATCATCTTTGTCGTGGTGGTGCTGGGCATC
ATCGCGCGGGTGGTCGGCTTCAACATCTTCCGCTTCGTTGCGTACATCAAGGAAGAACTGCTAATCGTGCTCGGCACCAG
CTCGTCGGAATCGGCGCTGCCGCACCTGATGGAAAAGATGGAGAAGCTGGGCTGCTCGAAGTCGGTGGTGGGCCTGGTGG
TTCCGACCGGTTACTCGTTCAATCTGGACGGCACCAACATCTACATGACGATGGCGGTGATCTTCATCTCGCAAGCACTG
AACATCGAGCTGACCTGGACGCAGCAGCTGACCATCCTGGCCGTTGCCATGCTGACGTCGAAGGGCGCATCGGGCATCAC
CGGTGCGGGCTTCATCACGCTGGCCGCAACGCTGGCCGTGGTGCCGGATATTCCGGTGGCGGGCATGGTGCTGATTCTCG
GTATCGACCGCTTCATGAGCGAATGCCGTGCCCTGACCAACATCACCGGCAACGGCGTGGCCTGCGTGGTGATCTCGGCC
TGGGAGCGTGAATTGGATCGCGCCAAACTGGCTCGCGTGCTGTCGGGCGACCGCAGCGACGAGGGCGTGCCGGCCACCCC
GGCAGTTTGA

Upstream 100 bases:

>100_bases
GACACATCAGCGTATCGAGGCAGGAGAGAGACCACACCAGGGCGGGTCGGACCGGGCGAGCAGCTGAGCGCACGGCCGGT
CTGTGACACTCGAGGAAGAC

Downstream 100 bases:

>100_bases
TCGATCGGTTCTAGCCGGCGCCTGCGGGCGCCGGCGCCTGAAAGCGCAAAGCGTATGATTGCGGGGCCTGTCCTTCCAGA
CGGCCCCGCGTCTTTTTTGG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKKPFYKILYVQVLAAIVIGVLLGHYSPELAVKMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALIIGLAAGHIFNPGAGFNVDVNTIDAKAVAQYAAKAHSASTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQISHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVISLVPLLKLVGTFYFTAIIFVVVVLGI
IARVVGFNIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTWTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPDIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRAKLARVLSGDRSDEGVPATPAV

Sequences:

>Translated_429_residues
MKKPFYKILYVQVLAAIVIGVLLGHYSPELAVKMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALIIGLAAGHIFNPGAGFNVDVNTIDAKAVAQYAAKAHSASTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQISHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVISLVPLLKLVGTFYFTAIIFVVVVLGI
IARVVGFNIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTWTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPDIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRAKLARVLSGDRSDEGVPATPAV
>Mature_429_residues
MKKPFYKILYVQVLAAIVIGVLLGHYSPELAVKMKPLGDGFIQLIKMVIGPIIFCTVVSGIAGMRDMKKVGRVGGKALLY
FEVVSTFALIIGLAAGHIFNPGAGFNVDVNTIDAKAVAQYAAKAHSASTVDFLLNIIPSTVVDAFAKGDILQILLIALLF
GGALSAMGERAQMVTDFIDQISHVFFRIVHVITRVAPIGAFGAMAFTIGKYGVISLVPLLKLVGTFYFTAIIFVVVVLGI
IARVVGFNIFRFVAYIKEELLIVLGTSSSESALPHLMEKMEKLGCSKSVVGLVVPTGYSFNLDGTNIYMTMAVIFISQAL
NIELTWTQQLTILAVAMLTSKGASGITGAGFITLAATLAVVPDIPVAGMVLILGIDRFMSECRALTNITGNGVACVVISA
WERELDRAKLARVLSGDRSDEGVPATPAV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=458, Percent_Identity=25.1091703056769, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI21314632, Length=438, Percent_Identity=25.7990867579909, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI4827012, Length=323, Percent_Identity=28.4829721362229, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI40254478, Length=448, Percent_Identity=25.8928571428571, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI66773030, Length=408, Percent_Identity=24.5098039215686, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI5032093, Length=374, Percent_Identity=26.4705882352941, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI194239697, Length=452, Percent_Identity=24.5575221238938, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468564, Length=251, Percent_Identity=27.4900398406374, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI223468566, Length=267, Percent_Identity=26.5917602996255, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI262359914, Length=406, Percent_Identity=24.1379310344828, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI301601644, Length=206, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789947, Length=422, Percent_Identity=61.6113744075829, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1790514, Length=413, Percent_Identity=40.6779661016949, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1788024, Length=408, Percent_Identity=20.0980392156863, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789473, Length=220, Percent_Identity=27.7272727272727, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=431, Percent_Identity=26.6821345707657, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=465, Percent_Identity=24.0860215053763, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=465, Percent_Identity=24.0860215053763, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI193206505, Length=451, Percent_Identity=24.6119733924612, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=428, Percent_Identity=22.6635514018692, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71996953, Length=417, Percent_Identity=23.021582733813, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=430, Percent_Identity=23.0232558139535, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=437, Percent_Identity=21.2814645308924, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137668, Length=437, Percent_Identity=21.2814645308924, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583023, Length=437, Percent_Identity=21.2814645308924, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281360481, Length=480, Percent_Identity=25.2083333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281360483, Length=480, Percent_Identity=25.2083333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137666, Length=480, Percent_Identity=25.2083333333333, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45638; Mature: 45638

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure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CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]