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The map label for this gene is gcvA [H]

Identifier: 187927289

GI number: 187927289

Start: 199864

End: 200826

Strand: Direct

Name: gcvA [H]

Synonym: Rpic_0181

Alternate gene names: 187927289

Gene position: 199864-200826 (Clockwise)

Preceding gene: 187927285

Following gene: 187927294

Centisome position: 5.07

GC content: 65.63

Gene sequence:

>963_bases
ATGACCGAAATCCGCGAACCGATGCGCTTGCCGTCACTTGGCGCGCTGAGGGTGTTCGAGGCGGCAGCCCGCTATGAGAG
TTTTTCGCGTGCAGCGACGGAGCTGTTCGTTACGCACGGCGCCGTCAGCCATCAGATGCGCACGCTGGAAGACGAGCTGG
GCGTGCCGTTGTTCGAGCGGCGGGGCAAGCGCGTCATGCTCACGCACGCGGGCCGCGCCTATGCCGATCGCGTGCGCGAG
GCGCTCGACCAGATCGCCCAGGCTACGCACCAACTGCGCGCCGGTAACCGAGACAACCGCCTGGCCATCAGCACGATGCC
GTCGTTTGCGGCGCGCTGGCTGACGCCGCATATCGGCACCTTCATCGAGCGCCATCCCGAGTTGGAAGTGGAACTGTTCT
CCAGCCCCGCGCTGGTCGATTTTGCGCGCGAAGAGGTGGACGTGGCGCTGCGCATGGGTTCGGGCAACTATCCGGGCCTG
TACGTGGAAAAGCTGCTCGACGATGTGTTCTTCCCGATCTGCAGCCCGTCGTTCAACGGCGGACGCCTGCCGCGCACGCT
GGCCGAGATGGCAAGCATGACCCTGCTGCGCAGCGAGGGCGAGGCATGGAAACCGTGGTTCGATGCGGCCGGCCTTGAGG
GGTTTGTGGAGCCGCGCGGGGGGCTGATGTTCCAGGATTCATCATTGCTGTTGCAGGCCGCGGCAGCGGGGCAGGGCATT
GCGCTGATCCGCCCGACGCTGGCGTTCAACGAATTGCTGGCCGGCCGCGTGGTGCGCCTGTTCGAGACGACGATCCCCTG
TCCGTGGAACTACTACTTCGTCTGTCCACACAGCGCGCTGCAGACGCCCAAGATGCAGGCGTTCCGCACGTGGCTGCTGC
CGGAGATTGCGGCGTTCAAGCTCAAGCTGGCGGGGCTGATGAACGACAACGCGGTGTGCCTGGGGCACGTGTCCAAGGGC
TGA

Upstream 100 bases:

>100_bases
TAGGCGCTTGGTTGCCTAGTACCAAACGATATATATTGCGTTTCTTGATAGTGCAGCTCACATAAAAATCACCCGGCGCT
ACGAAAATCCGGAGGGAAAG

Downstream 100 bases:

>100_bases
GCCCGGGCATCGGAAAAAATCCTCTACAAAGCAGAACGCCCCGGCATGACCGGGGCGTTTTGCGTTCTGCTGAGTGCTGC
GGAGGTGCGTTACAGCACGA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTEIREPMRLPSLGALRVFEAAARYESFSRAATELFVTHGAVSHQMRTLEDELGVPLFERRGKRVMLTHAGRAYADRVRE
ALDQIAQATHQLRAGNRDNRLAISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGL
YVEKLLDDVFFPICSPSFNGGRLPRTLAEMASMTLLRSEGEAWKPWFDAAGLEGFVEPRGGLMFQDSSLLLQAAAAGQGI
ALIRPTLAFNELLAGRVVRLFETTIPCPWNYYFVCPHSALQTPKMQAFRTWLLPEIAAFKLKLAGLMNDNAVCLGHVSKG

Sequences:

>Translated_320_residues
MTEIREPMRLPSLGALRVFEAAARYESFSRAATELFVTHGAVSHQMRTLEDELGVPLFERRGKRVMLTHAGRAYADRVRE
ALDQIAQATHQLRAGNRDNRLAISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGL
YVEKLLDDVFFPICSPSFNGGRLPRTLAEMASMTLLRSEGEAWKPWFDAAGLEGFVEPRGGLMFQDSSLLLQAAAAGQGI
ALIRPTLAFNELLAGRVVRLFETTIPCPWNYYFVCPHSALQTPKMQAFRTWLLPEIAAFKLKLAGLMNDNAVCLGHVSKG
>Mature_319_residues
TEIREPMRLPSLGALRVFEAAARYESFSRAATELFVTHGAVSHQMRTLEDELGVPLFERRGKRVMLTHAGRAYADRVREA
LDQIAQATHQLRAGNRDNRLAISTMPSFAARWLTPHIGTFIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLY
VEKLLDDVFFPICSPSFNGGRLPRTLAEMASMTLLRSEGEAWKPWFDAAGLEGFVEPRGGLMFQDSSLLLQAAAAGQGIA
LIRPTLAFNELLAGRVVRLFETTIPCPWNYYFVCPHSALQTPKMQAFRTWLLPEIAAFKLKLAGLMNDNAVCLGHVSKG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=40.7534246575342, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.2517006802721, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=30.4054054054054, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=28.6713286713287, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=27.5618374558304, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI157672245, Length=159, Percent_Identity=34.5911949685535, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=25.3571428571429, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787128, Length=152, Percent_Identity=32.2368421052632, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=254, Percent_Identity=25.5905511811024, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081978, Length=244, Percent_Identity=28.2786885245902, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790262, Length=238, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=24.5508982035928, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=26.0162601626016, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35622; Mature: 35490

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIREPMRLPSLGALRVFEAAARYESFSRAATELFVTHGAVSHQMRTLEDELGVPLFER
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RGKRVMLTHAGRAYADRVREALDQIAQATHQLRAGNRDNRLAISTMPSFAARWLTPHIGT
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCHHHHH
FIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLYVEKLLDDVFFPICSPSFNG
HHHCCCCCEEEECCCCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
GRLPRTLAEMASMTLLRSEGEAWKPWFDAAGLEGFVEPRGGLMFQDSSLLLQAAAAGQGI
CCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCE
ALIRPTLAFNELLAGRVVRLFETTIPCPWNYYFVCPHSALQTPKMQAFRTWLLPEIAAFK
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
LKLAGLMNDNAVCLGHVSKG
HHHHHHCCCCCEEEEECCCC
>Mature Secondary Structure 
TEIREPMRLPSLGALRVFEAAARYESFSRAATELFVTHGAVSHQMRTLEDELGVPLFER
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RGKRVMLTHAGRAYADRVREALDQIAQATHQLRAGNRDNRLAISTMPSFAARWLTPHIGT
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHCHHHHH
FIERHPELEVELFSSPALVDFAREEVDVALRMGSGNYPGLYVEKLLDDVFFPICSPSFNG
HHHCCCCCEEEECCCCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCC
GRLPRTLAEMASMTLLRSEGEAWKPWFDAAGLEGFVEPRGGLMFQDSSLLLQAAAAGQGI
CCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCE
ALIRPTLAFNELLAGRVVRLFETTIPCPWNYYFVCPHSALQTPKMQAFRTWLLPEIAAFK
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHH
LKLAGLMNDNAVCLGHVSKG
HHHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]