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The map label for this gene is menB [C]

Identifier: 187927226

GI number: 187927226

Start: 138194

End: 138988

Strand: Reverse

Name: menB [C]

Synonym: Rpic_0118

Alternate gene names: 187927226

Gene position: 138988-138194 (Counterclockwise)

Preceding gene: 187927227

Following gene: 187927225

Centisome position: 3.53

GC content: 68.43

Gene sequence:

>795_bases
ATGAACACTTTCGAGACCCTCGCCTTCGCACAGAACGGCGCCGTCGCCACGCTCACGCTGAACCGGCCCGACGTGCGCAA
CGCCTTCAACGAGACCGTCATCGCCGAGCTGACCGGCGCGTTCCGCGCGCTGGCCGATGAGCCGGGCGTGCGCGCCATCG
TCCTGACGGGCAACGGCCCGGCCTTCTGCGCGGGTGCCGACCTGAACTGGATGAAGAAGATGGCCGGCTACTCCGATGAC
GAGAACCGCGCCGACGCCCTGCGCCTGGCCGAGATGCTGCGCGCCATCTACACCTGCCCCAAGCCTGTGATCGCGCGCGT
GCAGGGCGACACGTATGCCGGCGGCGTCGGCCTGGTCGCCGCGTGCGACATCGTCGTTGCCGTGGACACGGCCAACTTCT
GCCTGTCGGAAGCGAAGCTCGGCTTGATCCCGGCCACCATCAGCCCGTACGTGATTCGCGCCCTGGGCGAGCAGGCGTCG
CGCCGCTACTTCATCACCGCAGAACGCTTTTCCGCTGCCGAAGCGCATCGCCTCGGCCTCGTGCATGAGCTGGTGAGCGC
AGACGCCCTCGACGCCAAGGTGGCCGACATCACCGCCGCGCTCGTCGCCAACAGCCCGAACGCGGTGCGCGAGAGCAAGC
GCCTGGTGCGTGAAGTCTCTGGCGCCGCCGTCAACGACGCCTTGCTGGCGGATACCGCTGAGCGCATCGCCGCCATCCGC
GCCTCGGAAGAAGGCCGCGAAGGCGTGCAAAGTTTTCTGGGCAAGCGCAAGCCGAACTGGCTGCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TTGGCTTGCCCGCCGGCCCGGTGCAAGCGGTGGCACCAGGCAGCAACGCTGAAGCATCCGGCGCAGACGACCCCAAGTCC
GACACCAACAAGGACCCCGA

Downstream 100 bases:

>100_bases
TCCGCACAACAACGAAACCACGCATCACTCGAAGGAAGTCCTCATGCAATCCACTCCGCTCAACTTCGTTCCAAACGCTG
CCAAGGCGCCCGTCACGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNTFETLAFAQNGAVATLTLNRPDVRNAFNETVIAELTGAFRALADEPGVRAIVLTGNGPAFCAGADLNWMKKMAGYSDD
ENRADALRLAEMLRAIYTCPKPVIARVQGDTYAGGVGLVAACDIVVAVDTANFCLSEAKLGLIPATISPYVIRALGEQAS
RRYFITAERFSAAEAHRLGLVHELVSADALDAKVADITAALVANSPNAVRESKRLVREVSGAAVNDALLADTAERIAAIR
ASEEGREGVQSFLGKRKPNWLLPA

Sequences:

>Translated_264_residues
MNTFETLAFAQNGAVATLTLNRPDVRNAFNETVIAELTGAFRALADEPGVRAIVLTGNGPAFCAGADLNWMKKMAGYSDD
ENRADALRLAEMLRAIYTCPKPVIARVQGDTYAGGVGLVAACDIVVAVDTANFCLSEAKLGLIPATISPYVIRALGEQAS
RRYFITAERFSAAEAHRLGLVHELVSADALDAKVADITAALVANSPNAVRESKRLVREVSGAAVNDALLADTAERIAAIR
ASEEGREGVQSFLGKRKPNWLLPA
>Mature_264_residues
MNTFETLAFAQNGAVATLTLNRPDVRNAFNETVIAELTGAFRALADEPGVRAIVLTGNGPAFCAGADLNWMKKMAGYSDD
ENRADALRLAEMLRAIYTCPKPVIARVQGDTYAGGVGLVAACDIVVAVDTANFCLSEAKLGLIPATISPYVIRALGEQAS
RRYFITAERFSAAEAHRLGLVHELVSADALDAKVADITAALVANSPNAVRESKRLVREVSGAAVNDALLADTAERIAAIR
ASEEGREGVQSFLGKRKPNWLLPA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=30.7392996108949, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=27.843137254902, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=27.843137254902, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=27.843137254902, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=27.5193798449612, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI70995211, Length=274, Percent_Identity=30.6569343065693, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594471, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI37594469, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=28.6259541984733, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=28.780487804878, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=31.5589353612167, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=31.3253012048193, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=30.6513409961686, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=31.0344827586207, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=27.3076923076923, Blast_Score=88, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=29.8850574712644, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=234, Percent_Identity=30.3418803418803, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=30.3738317757009, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=29.9107142857143, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=26.8, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=25.7777777777778, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=259, Percent_Identity=24.7104247104247, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=259, Percent_Identity=24.7104247104247, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=30.1115241635688, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=27.0916334661355, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=31.0204081632653, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=31.0204081632653, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=31.7180616740088, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=26.027397260274, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28029; Mature: 28029

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFETLAFAQNGAVATLTLNRPDVRNAFNETVIAELTGAFRALADEPGVRAIVLTGNGP
CCCHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AFCAGADLNWMKKMAGYSDDENRADALRLAEMLRAIYTCPKPVIARVQGDTYAGGVGLVA
EEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHH
ACDIVVAVDTANFCLSEAKLGLIPATISPYVIRALGEQASRRYFITAERFSAAEAHRLGL
HCEEEEEECCHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
VHELVSADALDAKVADITAALVANSPNAVRESKRLVREVSGAAVNDALLADTAERIAAIR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASEEGREGVQSFLGKRKPNWLLPA
CCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTFETLAFAQNGAVATLTLNRPDVRNAFNETVIAELTGAFRALADEPGVRAIVLTGNGP
CCCHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AFCAGADLNWMKKMAGYSDDENRADALRLAEMLRAIYTCPKPVIARVQGDTYAGGVGLVA
EEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCCHHHHH
ACDIVVAVDTANFCLSEAKLGLIPATISPYVIRALGEQASRRYFITAERFSAAEAHRLGL
HCEEEEEECCHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHH
VHELVSADALDAKVADITAALVANSPNAVRESKRLVREVSGAAVNDALLADTAERIAAIR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASEEGREGVQSFLGKRKPNWLLPA
CCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA