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The map label for this gene is bioB [H]

Identifier: 187927225

GI number: 187927225

Start: 137071

End: 138150

Strand: Reverse

Name: bioB [H]

Synonym: Rpic_0117

Alternate gene names: 187927225

Gene position: 138150-137071 (Counterclockwise)

Preceding gene: 187927226

Following gene: 187927224

Centisome position: 3.5

GC content: 64.81

Gene sequence:

>1080_bases
ATGCAATCCACTCCGCTCAACTTCGTTCCAAACGCTGCCAAGGCGCCCGTCACGCCCGGCCAGGCGCCCGACGCACGCTG
GAGCCGCGAAGCCATCGAAGCACTGTTCGCGCTGCCGTTCAACGACCTGCTGTTCCAGGCGCAGCAGGTGCACCGCGCCA
ACTTTGACGCGAACGCGGTGCAGCTATCGACGCTGCTGTCGATCAAGACCGGCGGCTGCCCGGAAGATTGCTCGTACTGC
CCGCAATCGGCGCGCTACGACACGGGTGTCGAGGCTGAAAAGCTGATGCCGATCGACGCCGTGCTGGAAGCCGCATCGCG
CGCCAAGCAAAACGGCGCAAGCCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCCAAGCCGCATCAGCTCGATGCCGTGG
CCGACATGGTGCGCGGCGTGAAGGCGATGGGGCTGGAGACCTGCGTGACGCTCGGCATGCTCAAGCAGGAACAGGCCGCG
CAGTTGAAGGAAGCGGGCCTGGACTACTACAACCACAACCTCGACACCGCGCCCGAGTTCTACGGCGAGATCATCACCAC
GCGCACGTATCAGGACCGGCTCGACACGCTCGAGCACGTGCGTGACGCCGGCATCAACGTGTGCTGCGGCGGCATCGTCG
GGCTGGGGGAATCGGTGCACGAGCGTGCGGGTCTGATCGCCGAACTGGCGAACATGGAGCCGTATCCGGACTCGGTGCCC
ATCAACAACCTGGTGAAGGTGGAAGGCACGCCGCTGGCGGGCAACGAAGAACTGGACCCGTTTGATTTCGTGCGCACGAT
TGCCGTGGCGCGGATCACGATGCCCAAGGCGATGGTGCGGCTCTCCGCTGGCCGCGAGGCGATGGACGATGCCTTGCAGG
CGCTGTGCTTCATGGCGGGCGCCAACTCGATCTTCTATGGCGAGAAGCTGCTGACGACGGATAACCCGGAGGCGGATGCG
GATCGGAAGCTGCTGGCGCGGTTGGGGATGCGGGTGGAGGTGCAGGATCACATGCACCATGAATCGCATGTGGGGGCGCA
GTCTTCGCATTGCCATATCGACATTACGCCCGCGGACTGA

Upstream 100 bases:

>100_bases
GAAGGCGTGCAAAGTTTTCTGGGCAAGCGCAAGCCGAACTGGCTGCTGCCGGCCTGATCCGCACAACAACGAAACCACGC
ATCACTCGAAGGAAGTCCTC

Downstream 100 bases:

>100_bases
TGCTTTGTTGTGGGGGTGGGTCTTGGTTTCTTGTTGGTGGTCTGCCTCCCTTTCGTCCCCTGCCGGGGCCGACTCACTTT
CTTTGTCTTGCCAAAGAAAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MQSTPLNFVPNAAKAPVTPGQAPDARWSREAIEALFALPFNDLLFQAQQVHRANFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDAVLEAASRAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAA
QLKEAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESVHERAGLIAELANMEPYPDSVP
INNLVKVEGTPLAGNEELDPFDFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHMHHESHVGAQSSHCHIDITPAD

Sequences:

>Translated_359_residues
MQSTPLNFVPNAAKAPVTPGQAPDARWSREAIEALFALPFNDLLFQAQQVHRANFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDAVLEAASRAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAA
QLKEAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESVHERAGLIAELANMEPYPDSVP
INNLVKVEGTPLAGNEELDPFDFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHMHHESHVGAQSSHCHIDITPAD
>Mature_359_residues
MQSTPLNFVPNAAKAPVTPGQAPDARWSREAIEALFALPFNDLLFQAQQVHRANFDANAVQLSTLLSIKTGGCPEDCSYC
PQSARYDTGVEAEKLMPIDAVLEAASRAKQNGASRFCMGAAWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAA
QLKEAGLDYYNHNLDTAPEFYGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESVHERAGLIAELANMEPYPDSVP
INNLVKVEGTPLAGNEELDPFDFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAGANSIFYGEKLLTTDNPEADA
DRKLLARLGMRVEVQDHMHHESHVGAQSSHCHIDITPAD

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=66.5573770491803, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321725, Length=319, Percent_Identity=51.0971786833856, Blast_Score=337, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39077; Mature: 39077

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTPLNFVPNAAKAPVTPGQAPDARWSREAIEALFALPFNDLLFQAQQVHRANFDANAV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHH
QLSTLLSIKTGGCPEDCSYCPQSARYDTGVEAEKLMPIDAVLEAASRAKQNGASRFCMGA
HHHHHHEECCCCCCHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
AWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAAQLKEAGLDYYNHNLDTAPEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHH
YGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESVHERAGLIAELANMEPYPDSVP
HHHHHHCCCHHHHHHHHHHHHHCCCCEEECHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
INNLVKVEGTPLAGNEELDPFDFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAG
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
ANSIFYGEKLLTTDNPEADADRKLLARLGMRVEVQDHMHHESHVGAQSSHCHIDITPAD
CCCEEECCEEECCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCEEEEEECCC
>Mature Secondary Structure
MQSTPLNFVPNAAKAPVTPGQAPDARWSREAIEALFALPFNDLLFQAQQVHRANFDANAV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHH
QLSTLLSIKTGGCPEDCSYCPQSARYDTGVEAEKLMPIDAVLEAASRAKQNGASRFCMGA
HHHHHHEECCCCCCHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
AWRNPKPHQLDAVADMVRGVKAMGLETCVTLGMLKQEQAAQLKEAGLDYYNHNLDTAPEF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHH
YGEIITTRTYQDRLDTLEHVRDAGINVCCGGIVGLGESVHERAGLIAELANMEPYPDSVP
HHHHHHCCCHHHHHHHHHHHHHCCCCEEECHHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
INNLVKVEGTPLAGNEELDPFDFVRTIAVARITMPKAMVRLSAGREAMDDALQALCFMAG
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
ANSIFYGEKLLTTDNPEADADRKLLARLGMRVEVQDHMHHESHVGAQSSHCHIDITPAD
CCCEEECCEEECCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]