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The map label for this gene is paaF [C]

Identifier: 187927210

GI number: 187927210

Start: 118803

End: 119582

Strand: Direct

Name: paaF [C]

Synonym: Rpic_0102

Alternate gene names: 187927210

Gene position: 118803-119582 (Clockwise)

Preceding gene: 187927206

Following gene: 187927211

Centisome position: 3.01

GC content: 67.56

Gene sequence:

>780_bases
GTGAGCACGGACCCGGAACTCCTCATCGAGCGGCGCGGCCAAGCGCTGTGGCTGACCATCCAGCGCGAAGACCGCCGCAA
CGCGATCAACGCCGCCGTGCTGGAAGCGCTGACTGCCGCGCTGGTCGACGCCGGCCGTGACGCCGCCGTGCGGGCCGTGG
TGCTGACCGGCACCGGCACGCGTGCCTTCTGTGCCGGCGCCGATCTGCAGACCGGACAGTCCTTCCGCTTCGACTATTCC
CAGCCGTACCAAGCCCTGGCGGATCTGTTTCGCCAGGCGCGCCGCTGCACCGTGCCGCTCATCGCCCGCGTGAATGGCGC
TTGCATGGCGGGCGGCATGGGTTTGCTGGCGATGTGCGATCTGGCGGTGGCAAGCCACAGCGCCACGTTCGGCTTGCCGG
AGGTGAAGGTCGGCGTGTTTCCCGCCCAGGTGCTGGCCGTGCTGCAGCACCAGTTGCCACGCCGCGTGCTGAACGAGTTG
TGCTTGACGGGCGAACCGATGAACGCAGACCGCGCGCTCGCCGCTGGCCTCGTCAACGAAGTCACCGAGCCCGAAGCGCT
TGATGGGGCTGTCGATGCATTGCTTGCGCGGCTGGTCGACAAATCGCCGTCGGCCATCCGGCGCGGCCTGTACACGCTCA
AGCACGTGGAATCGCTCAGCTTCGAGCAGGCCATGGCCTTTACCGAAAGCCAGATCGGTCTGTTTGCGTTGACCGAAGAC
GCGCAGGAAGGCCAAGCCGCCTTTCGCGAAAAGCGCGCCCCGAACTGGAGGGGCCGCTGA

Upstream 100 bases:

>100_bases
GAGGACCCACAGCACGCGAACGCTTCCATTTTTTCAATCAAATGATTTAGTATTGCCGGGCAGGCTGTCCAACCGAATTC
CCTTTCAAGGCGAGCGTCGC

Downstream 100 bases:

>100_bases
CATGCGCAATGACACCGTTTCCATCGGCGGGGCCAGCGGCTTCTGGGGCGACAGCGTGACCGGGCCGCTGCAGCTGGTGG
CCTCCGGCCGTCTCGACTTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSTDPELLIERRGQALWLTIQREDRRNAINAAVLEALTAALVDAGRDAAVRAVVLTGTGTRAFCAGADLQTGQSFRFDYS
QPYQALADLFRQARRCTVPLIARVNGACMAGGMGLLAMCDLAVASHSATFGLPEVKVGVFPAQVLAVLQHQLPRRVLNEL
CLTGEPMNADRALAAGLVNEVTEPEALDGAVDALLARLVDKSPSAIRRGLYTLKHVESLSFEQAMAFTESQIGLFALTED
AQEGQAAFREKRAPNWRGR

Sequences:

>Translated_259_residues
MSTDPELLIERRGQALWLTIQREDRRNAINAAVLEALTAALVDAGRDAAVRAVVLTGTGTRAFCAGADLQTGQSFRFDYS
QPYQALADLFRQARRCTVPLIARVNGACMAGGMGLLAMCDLAVASHSATFGLPEVKVGVFPAQVLAVLQHQLPRRVLNEL
CLTGEPMNADRALAAGLVNEVTEPEALDGAVDALLARLVDKSPSAIRRGLYTLKHVESLSFEQAMAFTESQIGLFALTED
AQEGQAAFREKRAPNWRGR
>Mature_258_residues
STDPELLIERRGQALWLTIQREDRRNAINAAVLEALTAALVDAGRDAAVRAVVLTGTGTRAFCAGADLQTGQSFRFDYSQ
PYQALADLFRQARRCTVPLIARVNGACMAGGMGLLAMCDLAVASHSATFGLPEVKVGVFPAQVLAVLQHQLPRRVLNELC
LTGEPMNADRALAAGLVNEVTEPEALDGAVDALLARLVDKSPSAIRRGLYTLKHVESLSFEQAMAFTESQIGLFALTEDA
QEGQAAFREKRAPNWRGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=28.9256198347107, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=29.1828793774319, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI70995211, Length=238, Percent_Identity=33.1932773109244, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4502327, Length=274, Percent_Identity=27.7372262773723, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI37594469, Length=207, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=207, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=32.3076923076923, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=31.9391634980989, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=32.4110671936759, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=28.4552845528455, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=31.0483870967742, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=270, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=28.6274509803922, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=31.4285714285714, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=25.1937984496124, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=28.110599078341, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=193, Percent_Identity=25.9067357512953, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=27.0531400966184, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=216, Percent_Identity=28.2407407407407, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=27.5590551181102, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20129971, Length=237, Percent_Identity=29.535864978903, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653477, Length=237, Percent_Identity=29.535864978903, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=27.0676691729323, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=269, Percent_Identity=27.5092936802974, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=26.4248704663212, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=282, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27949; Mature: 27818

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPELLIERRGQALWLTIQREDRRNAINAAVLEALTAALVDAGRDAAVRAVVLTGTGT
CCCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
RAFCAGADLQTGQSFRFDYSQPYQALADLFRQARRCTVPLIARVNGACMAGGMGLLAMCD
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCHHHHHHHH
LAVASHSATFGLPEVKVGVFPAQVLAVLQHQLPRRVLNELCLTGEPMNADRALAAGLVNE
HHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VTEPEALDGAVDALLARLVDKSPSAIRRGLYTLKHVESLSFEQAMAFTESQIGLFALTED
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AQEGQAAFREKRAPNWRGR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STDPELLIERRGQALWLTIQREDRRNAINAAVLEALTAALVDAGRDAAVRAVVLTGTGT
CCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCC
RAFCAGADLQTGQSFRFDYSQPYQALADLFRQARRCTVPLIARVNGACMAGGMGLLAMCD
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHCCHHHHHHHH
LAVASHSATFGLPEVKVGVFPAQVLAVLQHQLPRRVLNELCLTGEPMNADRALAAGLVNE
HHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VTEPEALDGAVDALLARLVDKSPSAIRRGLYTLKHVESLSFEQAMAFTESQIGLFALTED
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AQEGQAAFREKRAPNWRGR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA