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The map label for this gene is murD [H]

Identifier: 187918447

GI number: 187918447

Start: 612056

End: 613411

Strand: Direct

Name: murD [H]

Synonym: BH0585

Alternate gene names: 187918447

Gene position: 612056-613411 (Clockwise)

Preceding gene: 187918446

Following gene: 187918450

Centisome position: 66.36

GC content: 29.13

Gene sequence:

>1356_bases
GTGTATTTAAATGATATAAGAGGTAAAAATTTTTTGATTATGGGCTTGGGGCTTCATGGAGGAGGTCTTGCTGCTGCTAA
ATTTTTGTTGAAACATGGCGGTAATTTGGTAATTACTGATTTAAAGAGTGAGTTGGAATTAAGTCCAAGTATTAAGTCCT
TGAAGCAATTTAAAGATAAAGTTCGATATGTTTTAGGATATCATGATGAGGATGATTTTAAGAGCGCAGATATTGTTATT
AAAAATCCTGGTGTAAGCTTTGATAATAAGTATTTAAAGCTTGCAAAAAGAGTTGAGACCGATATTAGTTTATTTTTAAT
GTTTAATCAAAATCCGATAATTGCTATTACAGGAACTAAGGGAAAATCTACACTTGCATCTCTTTTGCATAAGGTTTTAG
TTGCTAAGTATCCGAATTCTAAGCTTGGAGGTAATATTGGGATATCTCCTTTAAGTTTTTTAGATGAACTTGATGGAACA
TCGCCTATTGTTTTAGAACTTTCTTCTTGGCAATTACATGATCTTGAAAATTTGTGTCCTATTATTAGTATTATTACAAA
TATTTATTGTGATCATCAAAATTATTATTCAAATTTTGATAGCTACATAGCAGATAAGTCAAAAATTTTTATAAATCAAA
ATTCCGGGATTTTGATCTCTCAGGATCAAGCTTATTATAATTATTTTTATAAATTTAAATCTAAATCCAAGCTCATTTTA
TTCTCAGAAACTGTGCCTTTAAACTTTAAAAATGATATTTTTTATTTTGAGATGGGTAAAGTTTATTTAAATAATAAAGT
GATGGGTACTCTTAGTGACTCAAGAATTGTGTTATTGATCTCTAAAATGATCACCGTTTTTATTGCAAGTTATTTACGTT
TGGATTTAAGTGTTGTATCTAAGATTTTGACTGATTTTGATGGTATTGAACATAGGTTAGAATTCGTAAGGGAATTTGAA
GGTGTTAAATATTATAATGATACGGCATCAACAATTCCTGATTCTACAGTTTTGTCTGTTAAAAGTTTAAGGCCCTACGG
AGGATTGATTAATCTTATTACTGGCGGGACTGATAAAGGGCTTGATTTTGCAGTTTTTGGTGAGATTTTAAGTATGGTTA
AAACTTGGATCTTATTAAGAGGAAGTGCTACTTTAAAGATTATTAAATTTTTAGAAGATAATAATCTTCATTATTTTATC
TTCTCTTCTTTAGAAGAATGTGTTTATTATGCTAAAAAAAACTCTATTCGGGATGATATAGTTTTATTTTCCCCAGCCAG
TGCTTCTTTTGAGCTTTTTAAGAATGAATTTGATAGAGGACTGCAATTTAAGAATTTAGTGAGTATTATGACTTAA

Upstream 100 bases:

>100_bases
AGATGTAATTAAGTTGGGTATTTCTCTTCCTTATTTTTATAGTACTAAGTGGATTAAGTCTATTAAAACAGGTTGATTAT
TTTGTTATAATACTGATGCT

Downstream 100 bases:

>100_bases
AATCTTTTTTTTATGATTTTATAGTAGATGTATCTTAGTATTTCAAGAATGCTATAAACTTTATATAAAAATTTTTTGTA
CACAAAGTCATAACATCCAA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MYLNDIRGKNFLIMGLGLHGGGLAAAKFLLKHGGNLVITDLKSELELSPSIKSLKQFKDKVRYVLGYHDEDDFKSADIVI
KNPGVSFDNKYLKLAKRVETDISLFLMFNQNPIIAITGTKGKSTLASLLHKVLVAKYPNSKLGGNIGISPLSFLDELDGT
SPIVLELSSWQLHDLENLCPIISIITNIYCDHQNYYSNFDSYIADKSKIFINQNSGILISQDQAYYNYFYKFKSKSKLIL
FSETVPLNFKNDIFYFEMGKVYLNNKVMGTLSDSRIVLLISKMITVFIASYLRLDLSVVSKILTDFDGIEHRLEFVREFE
GVKYYNDTASTIPDSTVLSVKSLRPYGGLINLITGGTDKGLDFAVFGEILSMVKTWILLRGSATLKIIKFLEDNNLHYFI
FSSLEECVYYAKKNSIRDDIVLFSPASASFELFKNEFDRGLQFKNLVSIMT

Sequences:

>Translated_451_residues
MYLNDIRGKNFLIMGLGLHGGGLAAAKFLLKHGGNLVITDLKSELELSPSIKSLKQFKDKVRYVLGYHDEDDFKSADIVI
KNPGVSFDNKYLKLAKRVETDISLFLMFNQNPIIAITGTKGKSTLASLLHKVLVAKYPNSKLGGNIGISPLSFLDELDGT
SPIVLELSSWQLHDLENLCPIISIITNIYCDHQNYYSNFDSYIADKSKIFINQNSGILISQDQAYYNYFYKFKSKSKLIL
FSETVPLNFKNDIFYFEMGKVYLNNKVMGTLSDSRIVLLISKMITVFIASYLRLDLSVVSKILTDFDGIEHRLEFVREFE
GVKYYNDTASTIPDSTVLSVKSLRPYGGLINLITGGTDKGLDFAVFGEILSMVKTWILLRGSATLKIIKFLEDNNLHYFI
FSSLEECVYYAKKNSIRDDIVLFSPASASFELFKNEFDRGLQFKNLVSIMT
>Mature_451_residues
MYLNDIRGKNFLIMGLGLHGGGLAAAKFLLKHGGNLVITDLKSELELSPSIKSLKQFKDKVRYVLGYHDEDDFKSADIVI
KNPGVSFDNKYLKLAKRVETDISLFLMFNQNPIIAITGTKGKSTLASLLHKVLVAKYPNSKLGGNIGISPLSFLDELDGT
SPIVLELSSWQLHDLENLCPIISIITNIYCDHQNYYSNFDSYIADKSKIFINQNSGILISQDQAYYNYFYKFKSKSKLIL
FSETVPLNFKNDIFYFEMGKVYLNNKVMGTLSDSRIVLLISKMITVFIASYLRLDLSVVSKILTDFDGIEHRLEFVREFE
GVKYYNDTASTIPDSTVLSVKSLRPYGGLINLITGGTDKGLDFAVFGEILSMVKTWILLRGSATLKIIKFLEDNNLHYFI
FSSLEECVYYAKKNSIRDDIVLFSPASASFELFKNEFDRGLQFKNLVSIMT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=469, Percent_Identity=27.7185501066098, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50970; Mature: 50970

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLNDIRGKNFLIMGLGLHGGGLAAAKFLLKHGGNLVITDLKSELELSPSIKSLKQFKDK
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
VRYVLGYHDEDDFKSADIVIKNPGVSFDNKYLKLAKRVETDISLFLMFNQNPIIAITGTK
HHEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLASLLHKVLVAKYPNSKLGGNIGISPLSFLDELDGTSPIVLELSSWQLHDLENLCP
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHCHHHHHH
IISIITNIYCDHQNYYSNFDSYIADKSKIFINQNSGILISQDQAYYNYFYKFKSKSKLIL
HHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHEEHHEEECCCCEEEE
FSETVPLNFKNDIFYFEMGKVYLNNKVMGTLSDSRIVLLISKMITVFIASYLRLDLSVVS
EECCCCCCCCCCEEEEEECEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KILTDFDGIEHRLEFVREFEGVKYYNDTASTIPDSTVLSVKSLRPYGGLINLITGGTDKG
HHHHHCCCHHHHHHHHHHHCCCEEECCCHHCCCCCCEEEECCCCCCCCEEEEEECCCCCC
LDFAVFGEILSMVKTWILLRGSATLKIIKFLEDNNLHYFIFSSLEECVYYAKKNSIRDDI
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCCE
VLFSPASASFELFKNEFDRGLQFKNLVSIMT
EEECCCCCHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MYLNDIRGKNFLIMGLGLHGGGLAAAKFLLKHGGNLVITDLKSELELSPSIKSLKQFKDK
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHH
VRYVLGYHDEDDFKSADIVIKNPGVSFDNKYLKLAKRVETDISLFLMFNQNPIIAITGTK
HHEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEEECCC
GKSTLASLLHKVLVAKYPNSKLGGNIGISPLSFLDELDGTSPIVLELSSWQLHDLENLCP
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHCHHHHHH
IISIITNIYCDHQNYYSNFDSYIADKSKIFINQNSGILISQDQAYYNYFYKFKSKSKLIL
HHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCEEEECCCHHEEHHEEECCCCEEEE
FSETVPLNFKNDIFYFEMGKVYLNNKVMGTLSDSRIVLLISKMITVFIASYLRLDLSVVS
EECCCCCCCCCCEEEEEECEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KILTDFDGIEHRLEFVREFEGVKYYNDTASTIPDSTVLSVKSLRPYGGLINLITGGTDKG
HHHHHCCCHHHHHHHHHHHCCCEEECCCHHCCCCCCEEEECCCCCCCCEEEEEECCCCCC
LDFAVFGEILSMVKTWILLRGSATLKIIKFLEDNNLHYFIFSSLEECVYYAKKNSIRDDI
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCCE
VLFSPASASFELFKNEFDRGLQFKNLVSIMT
EEECCCCCHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]