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The map label for this gene is exoA [H]

Identifier: 187918395

GI number: 187918395

Start: 548758

End: 549519

Strand: Direct

Name: exoA [H]

Synonym: BH0534

Alternate gene names: 187918395

Gene position: 548758-549519 (Clockwise)

Preceding gene: 187918394

Following gene: 187918396

Centisome position: 59.5

GC content: 32.55

Gene sequence:

>762_bases
ATGAATTTAATTTCTTGGAATGTCAATGGAATAAGAGCTGTTTTTGGTAAGGGTTTCTTTGAGTTTATTGAAAAATATAA
TCCGGATATCTTGTGTCTTCAGGAAACCAAGGCTTGCAAAGAGCAGTTACCAAAAGAGCTTATTAGCATTGAGGGGTATT
ATTCTTATTTTTCAAAATCCATAATCAAGGGTTATAGTGGGGTTGCTCTTTATTCAAAGATTGAGCCTATTAAATTAGAG
AATATGAGAATGGAAATATTTGATAGAGAAGGGAGATGTCTTATTGCTCATTATCGTGATTTTATCCTTATTAATGCGTA
TTTTCCTAATTCCCAAGCTTTAAGAAAAAGACTTAATTATAAGCTTGAATTTTTAATGAGCCTTGAATCTATTGCAAATT
CATTTGTAACTGCTGGAAAGAATGTTGTTATTTGTGGTGATTTTAATATTGCGCATACAGAAATTGATCTTTCTAATCCT
AAGTCCAGCAGAGAATCTGCTGGTTTTTATATTGAAGAGACTACTTGGATGGATAACTTTTTAAATGGAGGTTATGTAGA
TACCTTTAGAATGTTTAACGGGGAGCCAGGTAACTATACTTGGTGGGATTATAGAACAAGGGCAAGAGAGCGTAATATAG
GTTGGAGGATTGATTATTTTCTTGTAAATAAAGTCTTTAAATCTGAGGTAAGGGATGCTTTAATTTTAAATGAGGTAATG
GGTAGCGATCATTGTCCTGTTTTTTTAGAGTTAAAGCAGTAA

Upstream 100 bases:

>100_bases
GATATCCTGCATGAGGAATTTGATTATCTAAAGAGAGATGATATTTATTTGGCTTATGATGGGCTGCAGATACATGTTTA
ACTTGAATTGGTAAATAGTT

Downstream 100 bases:

>100_bases
AGTGTGTGTATGGAAGATGTATATGAGTAGATATTTTATAACAATTCTGTTTTTTTTTATTCATGGTTTTATTTTTGGGA
TTAATTTAGAGAGTGTTGAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNLISWNVNGIRAVFGKGFFEFIEKYNPDILCLQETKACKEQLPKELISIEGYYSYFSKSIIKGYSGVALYSKIEPIKLE
NMRMEIFDREGRCLIAHYRDFILINAYFPNSQALRKRLNYKLEFLMSLESIANSFVTAGKNVVICGDFNIAHTEIDLSNP
KSSRESAGFYIEETTWMDNFLNGGYVDTFRMFNGEPGNYTWWDYRTRARERNIGWRIDYFLVNKVFKSEVRDALILNEVM
GSDHCPVFLELKQ

Sequences:

>Translated_253_residues
MNLISWNVNGIRAVFGKGFFEFIEKYNPDILCLQETKACKEQLPKELISIEGYYSYFSKSIIKGYSGVALYSKIEPIKLE
NMRMEIFDREGRCLIAHYRDFILINAYFPNSQALRKRLNYKLEFLMSLESIANSFVTAGKNVVICGDFNIAHTEIDLSNP
KSSRESAGFYIEETTWMDNFLNGGYVDTFRMFNGEPGNYTWWDYRTRARERNIGWRIDYFLVNKVFKSEVRDALILNEVM
GSDHCPVFLELKQ
>Mature_253_residues
MNLISWNVNGIRAVFGKGFFEFIEKYNPDILCLQETKACKEQLPKELISIEGYYSYFSKSIIKGYSGVALYSKIEPIKLE
NMRMEIFDREGRCLIAHYRDFILINAYFPNSQALRKRLNYKLEFLMSLESIANSFVTAGKNVVICGDFNIAHTEIDLSNP
KSSRESAGFYIEETTWMDNFLNGGYVDTFRMFNGEPGNYTWWDYRTRARERNIGWRIDYFLVNKVFKSEVRDALILNEVM
GSDHCPVFLELKQ

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=38.28125, Blast_Score=184, Evalue=5e-47,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=38.28125, Blast_Score=184, Evalue=5e-47,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=38.28125, Blast_Score=184, Evalue=5e-47,
Organism=Homo sapiens, GI18375507, Length=311, Percent_Identity=31.5112540192926, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=29.1970802919708, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=36.1867704280156, Blast_Score=163, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71989539, Length=115, Percent_Identity=38.2608695652174, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319452, Length=349, Percent_Identity=23.4957020057307, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=44.8818897637795, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=44.8818897637795, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29570; Mature: 29570

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLISWNVNGIRAVFGKGFFEFIEKYNPDILCLQETKACKEQLPKELISIEGYYSYFSKS
CCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
IIKGYSGVALYSKIEPIKLENMRMEIFDREGRCLIAHYRDFILINAYFPNSQALRKRLNY
HHCCCCCEEEECCCCCEEECCEEEEEECCCCCEEEEECCCEEEEEEECCCHHHHHHHHCH
KLEFLMSLESIANSFVTAGKNVVICGDFNIAHTEIDLSNPKSSRESAGFYIEETTWMDNF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHH
LNGGYVDTFRMFNGEPGNYTWWDYRTRARERNIGWRIDYFLVNKVFKSEVRDALILNEVM
CCCCEEEEEEEECCCCCCEEEECHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
GSDHCPVFLELKQ
CCCCCCEEEEECC
>Mature Secondary Structure
MNLISWNVNGIRAVFGKGFFEFIEKYNPDILCLQETKACKEQLPKELISIEGYYSYFSKS
CCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
IIKGYSGVALYSKIEPIKLENMRMEIFDREGRCLIAHYRDFILINAYFPNSQALRKRLNY
HHCCCCCEEEECCCCCEEECCEEEEEECCCCCEEEEECCCEEEEEEECCCHHHHHHHHCH
KLEFLMSLESIANSFVTAGKNVVICGDFNIAHTEIDLSNPKSSRESAGFYIEETTWMDNF
HHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHH
LNGGYVDTFRMFNGEPGNYTWWDYRTRARERNIGWRIDYFLVNKVFKSEVRDALILNEVM
CCCCEEEEEEEECCCCCCEEEECHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
GSDHCPVFLELKQ
CCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]