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The map label for this gene is pheS [H]

Identifier: 187918378

GI number: 187918378

Start: 528911

End: 530458

Strand: Direct

Name: pheS [H]

Synonym: BH0513

Alternate gene names: 187918378

Gene position: 528911-530458 (Clockwise)

Preceding gene: 187918377

Following gene: 187918379

Centisome position: 57.35

GC content: 33.01

Gene sequence:

>1548_bases
GTGGAGAATAAATTTGAGGTAGTAAAGACATTACATCCCCTTGAGATAAAAGTGATTTTGAATTATAAAGAGGGAGATGA
TATTTTTGCTTTAAGGCTTGCTGCAGACTTACTTTATAATGAGGGGCAATCAAATAAAACAATTGAATGGCTTGTTTCTA
AAGGTATTCTTAGAGAAACTTTTAGAAAGCTTAATGTGTTTTATCGTCTAACTGAAAAAGGTATTGATGCTTTAGATAAT
GGTTTAATTGAAGAGAAAATAATCAGTCTTGTATCTAGAAAGACAGTTTTCGTTGCCAATTTGTCATCAGAGTTAGAAAT
TGATGCTAGAGATGCTGGTAAGGCATTTGGCAATTTATCTAAAGAGGGAGTTTTGTCTTTGGGATTGGGTAAGGAAATTA
TTGTGAAAGATTTAAATCGTGCTAATTACAAGATAGTTAAAGAATTGCTTGTAAAAGCTAAAGATGGTGATCTCTTAGAA
GATGATTTATCCCAAGATGAATTGTTAGTCATTTCTAATTATTCTAAGAAGAAAGGTGCTAGTGATGTGTTATTTAAAGT
AATAGAGAAATTAGACTTAAAATTTGAATTTTCTAAGTTTGGATTAGAAGTAAAATCCGAACTTAGAGAGAGTAATTTAA
CAGGTGATGAGATTGTAAAGTTGACCCCTGAGCTTTTAAAAAATAAAGCTTATGAGAATAAAAATTTTAGGGCTTATAAT
ATTCATGTTTCATCAAGTAAGACCTTTATTGGACGTGCAAATCCTTATTCAGAGTATATTGCTAAAGTTAAGGATAAACT
TGTAAGTCTTGGTTTTGAAGAATTTGATGGTCCCTTAGTTGAGAGTGAGTTTTTTAATAATGATGCTCTTTTTATGCCTC
AATTTCATCCTGCACGTGATGTGAGAGATGTTTATTATATTAAAGACCCAAGCTCACTTTTATCTTTGCCTGAGCCTTAT
TTTTCTAATGTTAAGGCTGTTCATGAGAATGGTTACACTACAGGTTCAAGAGGTTGGAGGTATGATTTTAGCGAGAGTAT
TTCAAAAAGATTAGTGCTTAGAACTCAAGGTACAGTGCTCTCAGCAAAGCAATTAATTAATGCAAAAAATCCTGGCAAAT
ATTTTGGAATTGTTAGGTGTTTTAGATACGATCAAGTTGATGCTACTCATGGAGCTGATTTTTATCAAACAGAAGGAATT
GTTATTGGAGATGTTAATATTAAGATTTTACTGGGTCTTCTTGAAATTTTTGCTAAAGAATTGGCAGGTGCTACTGAGGT
TAAATATGTTCCTGCTTATTTTCCATTTACAGAGCCTTCAATTGAAGTGCATGTAAAGCATCCTGTGCTTGGTTGGTTTG
AATTAGGTGGGAGTGGTATTTTCAGACCAGAGGTTACAAAACCTTTTGGAATTGATGTGCCCGTTATTGCTTGGGGTATT
GGTATTGATAGAATGGCTTTAATGCATTTAGGTTTAAGTGATTTAAGGGAGCTTTTTACCCATGATATTGGTGATGTTGT
ATTAAGGCGGGGAAAAGTAAATGCCTAA

Upstream 100 bases:

>100_bases
CCTTGGAAATTTCAAGAGCTACTAAATTATCTGTTGGAGAGGTTGAGCTTATTTTGGAACTTGGAATTATTAGTAAAAGT
GATGATTAAGGGATAGTTTT

Downstream 100 bases:

>100_bases
AGTTGAAGTTTATAGAAGTATTTTATTAGGAAAGATAGGAAAAGATTTAACGGATTGTGAGCTTGTATCTATTCTTGAAA
TGGCTAAGGCTGAAATTTGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MENKFEVVKTLHPLEIKVILNYKEGDDIFALRLAADLLYNEGQSNKTIEWLVSKGILRETFRKLNVFYRLTEKGIDALDN
GLIEEKIISLVSRKTVFVANLSSELEIDARDAGKAFGNLSKEGVLSLGLGKEIIVKDLNRANYKIVKELLVKAKDGDLLE
DDLSQDELLVISNYSKKKGASDVLFKVIEKLDLKFEFSKFGLEVKSELRESNLTGDEIVKLTPELLKNKAYENKNFRAYN
IHVSSSKTFIGRANPYSEYIAKVKDKLVSLGFEEFDGPLVESEFFNNDALFMPQFHPARDVRDVYYIKDPSSLLSLPEPY
FSNVKAVHENGYTTGSRGWRYDFSESISKRLVLRTQGTVLSAKQLINAKNPGKYFGIVRCFRYDQVDATHGADFYQTEGI
VIGDVNIKILLGLLEIFAKELAGATEVKYVPAYFPFTEPSIEVHVKHPVLGWFELGGSGIFRPEVTKPFGIDVPVIAWGI
GIDRMALMHLGLSDLRELFTHDIGDVVLRRGKVNA

Sequences:

>Translated_515_residues
MENKFEVVKTLHPLEIKVILNYKEGDDIFALRLAADLLYNEGQSNKTIEWLVSKGILRETFRKLNVFYRLTEKGIDALDN
GLIEEKIISLVSRKTVFVANLSSELEIDARDAGKAFGNLSKEGVLSLGLGKEIIVKDLNRANYKIVKELLVKAKDGDLLE
DDLSQDELLVISNYSKKKGASDVLFKVIEKLDLKFEFSKFGLEVKSELRESNLTGDEIVKLTPELLKNKAYENKNFRAYN
IHVSSSKTFIGRANPYSEYIAKVKDKLVSLGFEEFDGPLVESEFFNNDALFMPQFHPARDVRDVYYIKDPSSLLSLPEPY
FSNVKAVHENGYTTGSRGWRYDFSESISKRLVLRTQGTVLSAKQLINAKNPGKYFGIVRCFRYDQVDATHGADFYQTEGI
VIGDVNIKILLGLLEIFAKELAGATEVKYVPAYFPFTEPSIEVHVKHPVLGWFELGGSGIFRPEVTKPFGIDVPVIAWGI
GIDRMALMHLGLSDLRELFTHDIGDVVLRRGKVNA
>Mature_515_residues
MENKFEVVKTLHPLEIKVILNYKEGDDIFALRLAADLLYNEGQSNKTIEWLVSKGILRETFRKLNVFYRLTEKGIDALDN
GLIEEKIISLVSRKTVFVANLSSELEIDARDAGKAFGNLSKEGVLSLGLGKEIIVKDLNRANYKIVKELLVKAKDGDLLE
DDLSQDELLVISNYSKKKGASDVLFKVIEKLDLKFEFSKFGLEVKSELRESNLTGDEIVKLTPELLKNKAYENKNFRAYN
IHVSSSKTFIGRANPYSEYIAKVKDKLVSLGFEEFDGPLVESEFFNNDALFMPQFHPARDVRDVYYIKDPSSLLSLPEPY
FSNVKAVHENGYTTGSRGWRYDFSESISKRLVLRTQGTVLSAKQLINAKNPGKYFGIVRCFRYDQVDATHGADFYQTEGI
VIGDVNIKILLGLLEIFAKELAGATEVKYVPAYFPFTEPSIEVHVKHPVLGWFELGGSGIFRPEVTKPFGIDVPVIAWGI
GIDRMALMHLGLSDLRELFTHDIGDVVLRRGKVNA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=335, Percent_Identity=34.6268656716418, Blast_Score=199, Evalue=6e-51,
Organism=Escherichia coli, GI1788007, Length=259, Percent_Identity=28.957528957529, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32563657, Length=293, Percent_Identity=37.8839590443686, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17508957, Length=293, Percent_Identity=37.8839590443686, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32566635, Length=231, Percent_Identity=26.8398268398268, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321087, Length=465, Percent_Identity=32.0430107526882, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI18858079, Length=290, Percent_Identity=36.8965517241379, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319 [H]

Pfam domain/function: PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 58085; Mature: 58085

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKFEVVKTLHPLEIKVILNYKEGDDIFALRLAADLLYNEGQSNKTIEWLVSKGILRET
CCCHHHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FRKLNVFYRLTEKGIDALDNGLIEEKIISLVSRKTVFVANLSSELEIDARDAGKAFGNLS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCCEECCHHHHHHHCCCC
KEGVLSLGLGKEIIVKDLNRANYKIVKELLVKAKDGDLLEDDLSQDELLVISNYSKKKGA
CCCEEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCCCCH
SDVLFKVIEKLDLKFEFSKFGLEVKSELRESNLTGDEIVKLTPELLKNKAYENKNFRAYN
HHHHHHHHHHHCCEEEHHHHCHHHHHHHHHCCCCHHHHHHCCHHHHHCCCCCCCCEEEEE
IHVSSSKTFIGRANPYSEYIAKVKDKLVSLGFEEFDGPLVESEFFNNDALFMPQFHPARD
EEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHHCCCCEECHHHCCCCCEECCCCCCCCC
VRDVYYIKDPSSLLSLPEPYFSNVKAVHENGYTTGSRGWRYDFSESISKRLVLRTQGTVL
CCEEEEEECCHHHHCCCCHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEECCCHH
SAKQLINAKNPGKYFGIVRCFRYDQVDATHGADFYQTEGIVIGDVNIKILLGLLEIFAKE
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHHH
LAGATEVKYVPAYFPFTEPSIEVHVKHPVLGWFELGGSGIFRPEVTKPFGIDVPVIAWGI
HCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEECC
GIDRMALMHLGLSDLRELFTHDIGDVVLRRGKVNA
CHHHHHHHHCCHHHHHHHHHHCHHHHHHHCCCCCC
>Mature Secondary Structure
MENKFEVVKTLHPLEIKVILNYKEGDDIFALRLAADLLYNEGQSNKTIEWLVSKGILRET
CCCHHHHHHCCCCEEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FRKLNVFYRLTEKGIDALDNGLIEEKIISLVSRKTVFVANLSSELEIDARDAGKAFGNLS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCCCEECCHHHHHHHCCCC
KEGVLSLGLGKEIIVKDLNRANYKIVKELLVKAKDGDLLEDDLSQDELLVISNYSKKKGA
CCCEEEECCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCCCCCH
SDVLFKVIEKLDLKFEFSKFGLEVKSELRESNLTGDEIVKLTPELLKNKAYENKNFRAYN
HHHHHHHHHHHCCEEEHHHHCHHHHHHHHHCCCCHHHHHHCCHHHHHCCCCCCCCEEEEE
IHVSSSKTFIGRANPYSEYIAKVKDKLVSLGFEEFDGPLVESEFFNNDALFMPQFHPARD
EEEECCCEEEECCCCHHHHHHHHHHHHHHCCHHHCCCCEECHHHCCCCCEECCCCCCCCC
VRDVYYIKDPSSLLSLPEPYFSNVKAVHENGYTTGSRGWRYDFSESISKRLVLRTQGTVL
CCEEEEEECCHHHHCCCCHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHEEEEEECCCHH
SAKQLINAKNPGKYFGIVRCFRYDQVDATHGADFYQTEGIVIGDVNIKILLGLLEIFAKE
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCEEECCEEEECCHHHHHHHHHHHHHHH
LAGATEVKYVPAYFPFTEPSIEVHVKHPVLGWFELGGSGIFRPEVTKPFGIDVPVIAWGI
HCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEECC
GIDRMALMHLGLSDLRELFTHDIGDVVLRRGKVNA
CHHHHHHHHCCHHHHHHHHHHCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]