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The map label for this gene is tuf [H]

Identifier: 187918342

GI number: 187918342

Start: 500276

End: 501460

Strand: Direct

Name: tuf [H]

Synonym: BH0476

Alternate gene names: 187918342

Gene position: 500276-501460 (Clockwise)

Preceding gene: 187918340

Following gene: 187918343

Centisome position: 54.24

GC content: 37.47

Gene sequence:

>1185_bases
ATGGCTAAGGAAGTTTTTCAGAGAACAAAGCCGCATATGAATGTGGGTACAATAGGGCATGTTGACCATGGTAAGACAAC
ATTAACAGCAGCTATTAGTATTTATTGTTCAAAGGTAAATAAAGATGCTAAGGCGCTCAAGTATGAAGATATTGATAATG
CGCCTGAAGAGAAAGCAAGAGGAATAACAATTAATGCCAGACATATTGAGTATGAAACTGCTGGTAGGCATTATGCTCAC
GTTGATTGTCCCGGACACGCTGACTATATTAAAAATATGATTACAGGTGCAGCTCAGATGGATGCGGCAATATTGCTGGT
TGCTGCTGATAGTGGAGCAGAGCCTCAGACAAAAGAACATTTACTTCTTGCGCAGCGAATGGGAATAAAGAAAATAATAG
TGTTTTTAAATAAATTAGATTTAGCAGATCCTGAACTTGTTGAACTTGTTGAAGTTGAAGTTTTGGAACTTGTTGAAAAA
TATGGATTCCCTGGCGATACACCAATAGTCAAGGGTTCGGCTTTTGGGGCTATGTCAAATCCTGATGATCCTGAAGCTAC
AAAATGCATAAAGGAACTTCTTGAAACTATGGATAGTTATTTTGATCTTCCTGAGAGAGATATTGATAAGCCATTTTTGC
TTGCTATTGAAGATGTTTTCTCTATCTCTGGACGTGGTACTGTTGCTACTGGTCGTATTGAAAGAGGTATTATTAAGGTT
GGACAAGAAGTTGAAATCGTTGGAATTAGAGAAACCAGAAAGACAACTGTTACTGGTGTTGAAATGTTTCAAAAGATTCT
TGAGCAAGGTGAGGCAGGGGATAATGTTGGTCTTTTGTTAAGAGGTGTTGATAAAAAGGATGTTGAGAGAGGTCAGGTTA
TTGCTGCTATTGGTACAATTACTCCTCATAAAAAGTTCAAGGCTTCTATATATTGTCTAACTAAGGAAGAAGGCGGAAGA
CATAAACCATTCTTTTCAGGATATAGACCACAGTTTTTCTTTAGAACAACTGATGTTACAGGTATGGTTAGCTTAGAGGG
CAAGGAAATGGTTATGCCTGGAGATAATGTTGACATTGTTGTTGAACTTATATCTTTAATAGCTATGGATAAAAATGTTG
AGTTTGCTGTTAGAGAAGGTGGTAGAACAGTTGCTTCAGGAAGGATTCTTGAAATATTGGAATAG

Upstream 100 bases:

>100_bases
ATGATTGTTGTATCTTAATGAAGAAACAAGGTAGAATTCTTAAAATAAGAGTTTTGAAGAATAGGTAAAAATTGAGAGTA
TAAATTTTGGGAGGTGATTC

Downstream 100 bases:

>100_bases
TGTAGGGTTTAGAGGTTTTTATATCTCTAAAGTTTAGGAGAATAAATTGATTACTAAAGATAAGATACGGGTAAGACTTT
TTAGTTTTGATGTTAAGATA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKVNKDAKALKYEDIDNAPEEKARGITINARHIEYETAGRHYAH
VDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEK
YGFPGDTPIVKGSAFGAMSNPDDPEATKCIKELLETMDSYFDLPERDIDKPFLLAIEDVFSISGRGTVATGRIERGIIKV
GQEVEIVGIRETRKTTVTGVEMFQKILEQGEAGDNVGLLLRGVDKKDVERGQVIAAIGTITPHKKFKASIYCLTKEEGGR
HKPFFSGYRPQFFFRTTDVTGMVSLEGKEMVMPGDNVDIVVELISLIAMDKNVEFAVREGGRTVASGRILEILE

Sequences:

>Translated_394_residues
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKVNKDAKALKYEDIDNAPEEKARGITINARHIEYETAGRHYAH
VDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEK
YGFPGDTPIVKGSAFGAMSNPDDPEATKCIKELLETMDSYFDLPERDIDKPFLLAIEDVFSISGRGTVATGRIERGIIKV
GQEVEIVGIRETRKTTVTGVEMFQKILEQGEAGDNVGLLLRGVDKKDVERGQVIAAIGTITPHKKFKASIYCLTKEEGGR
HKPFFSGYRPQFFFRTTDVTGMVSLEGKEMVMPGDNVDIVVELISLIAMDKNVEFAVREGGRTVASGRILEILE
>Mature_393_residues
AKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKVNKDAKALKYEDIDNAPEEKARGITINARHIEYETAGRHYAHV
DCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEHLLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKY
GFPGDTPIVKGSAFGAMSNPDDPEATKCIKELLETMDSYFDLPERDIDKPFLLAIEDVFSISGRGTVATGRIERGIIKVG
QEVEIVGIRETRKTTVTGVEMFQKILEQGEAGDNVGLLLRGVDKKDVERGQVIAAIGTITPHKKFKASIYCLTKEEGGRH
KPFFSGYRPQFFFRTTDVTGMVSLEGKEMVMPGDNVDIVVELISLIAMDKNVEFAVREGGRTVASGRILEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=51.3924050632911, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI4503475, Length=326, Percent_Identity=34.9693251533742, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=34.9693251533742, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.3854875283447, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.3854875283447, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI54607086, Length=322, Percent_Identity=30.7453416149068, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=26.1467889908257, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=26.1467889908257, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=26.1467889908257, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI46094014, Length=436, Percent_Identity=26.3761467889908, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4503507, Length=280, Percent_Identity=30.7142857142857, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI157426893, Length=380, Percent_Identity=22.8947368421053, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=64.1414141414141, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=64.1414141414141, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=31.1572700296736, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789108, Length=332, Percent_Identity=27.4096385542169, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=29.3478260869565, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=34.0425531914894, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=49.7512437810945, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=41.4507772020725, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17552884, Length=328, Percent_Identity=37.1951219512195, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=328, Percent_Identity=37.1951219512195, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=312, Percent_Identity=34.6153846153846, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=31.9852941176471, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=26.6203703703704, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=26.6203703703704, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=340, Percent_Identity=27.3529411764706, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=39.3103448275862, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=455, Percent_Identity=26.8131868131868, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.8481012658228, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71994658, Length=233, Percent_Identity=31.3304721030043, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=56.1712846347607, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6325337, Length=347, Percent_Identity=34.0057636887608, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=347, Percent_Identity=34.0057636887608, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=24.8351648351648, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=280, Percent_Identity=30.3571428571429, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=324, Percent_Identity=28.3950617283951, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=136, Percent_Identity=32.3529411764706, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=53.4177215189873, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=53.4177215189873, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=48.7113402061856, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=326, Percent_Identity=34.3558282208589, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=326, Percent_Identity=34.3558282208589, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=326, Percent_Identity=34.3558282208589, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=326, Percent_Identity=34.3558282208589, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=326, Percent_Identity=34.6625766871166, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=326, Percent_Identity=34.6625766871166, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=345, Percent_Identity=28.9855072463768, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137380, Length=441, Percent_Identity=27.6643990929705, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=30.0813008130081, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=30.0813008130081, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43360; Mature: 43229

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKVNKDAKALKYEDIDNAPEEKAR
CCHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHC
GITINARHIEYETAGRHYAHVDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHH
LLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKYGFPGDTPIVKGSAFGAMSN
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
PDDPEATKCIKELLETMDSYFDLPERDIDKPFLLAIEDVFSISGRGTVATGRIERGIIKV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCEEEECHHHHHHHHC
GQEVEIVGIRETRKTTVTGVEMFQKILEQGEAGDNVGLLLRGVDKKDVERGQVIAAIGTI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEEEECC
TPHKKFKASIYCLTKEEGGRHKPFFSGYRPQFFFRTTDVTGMVSLEGKEMVMPGDNVDIV
CCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCHHHH
VELISLIAMDKNVEFAVREGGRTVASGRILEILE
HHHHHHHHCCCCCEEEEECCCCEEECCCEEEECC
>Mature Secondary Structure 
AKEVFQRTKPHMNVGTIGHVDHGKTTLTAAISIYCSKVNKDAKALKYEDIDNAPEEKAR
CHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHC
GITINARHIEYETAGRHYAHVDCPGHADYIKNMITGAAQMDAAILLVAADSGAEPQTKEH
CEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCEEEEEEEECCCCCCCHHHH
LLLAQRMGIKKIIVFLNKLDLADPELVELVEVEVLELVEKYGFPGDTPIVKGSAFGAMSN
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
PDDPEATKCIKELLETMDSYFDLPERDIDKPFLLAIEDVFSISGRGTVATGRIERGIIKV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCEEEECHHHHHHHHC
GQEVEIVGIRETRKTTVTGVEMFQKILEQGEAGDNVGLLLRGVDKKDVERGQVIAAIGTI
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCEEEEEEECC
TPHKKFKASIYCLTKEEGGRHKPFFSGYRPQFFFRTTDVTGMVSLEGKEMVMPGDNVDIV
CCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCEEECCCCCHHHH
VELISLIAMDKNVEFAVREGGRTVASGRILEILE
HHHHHHHHCCCCCEEEEECCCCEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA