Click here to switch to the map view.

The map label for this gene is capI [H]

Identifier: 187918310

GI number: 187918310

Start: 467517

End: 468575

Strand: Direct

Name: capI [H]

Synonym: BH0444

Alternate gene names: 187918310

Gene position: 467517-468575 (Clockwise)

Preceding gene: 187918309

Following gene: 187918311

Centisome position: 50.69

GC content: 31.82

Gene sequence:

>1059_bases
ATGAGAGTGTTTTTAACAGGTATTGCAGGATTTATTGGATTTCATGTTGCCAAGAGGCTTGCTGATAATGGGCATGAGGT
TTTAGGTGTTGATGTTATAAATGATTATTATGAACCTAATCTGAAATATGAAAGGTTAAAAGCTTTAGGGTTTGATCGTG
AGAATATATGTGGTAAAAAAATTCTTCAAAGCAATAAATATGATAATTTAAGTTTTATTTGTCTTGATATTTTAAATAAA
GAGCAAGTCTTATCTCTTTTTTCTAGGTATAAATTTACGCATGTTTGTCATTTAGCAGCTCAGGCCGGTATTAGAGATAG
TATTGAAAATCCTGATAGTTATATTTCAGTTAATATTGTTGGATTTTTTAATGTTTTAGATGCATGCAGAATACATAAAA
GTCATATTGAGCATTTTGTTTATGCCTCAACATCTGCAGTGTATGGAATAAATGAAAAGATGCCTTCAGATGAGGATAGC
ATTACAGATCATCCTTTGAATTTATATGCAGCTAGCAAAAAATCTAATGAGGTCATAGCACATGCTTACAGTTCATCTTT
TAACATTCCGACAACAGGATTAAGATTTTTTACGGTTTATGGGCCTTATGGACGACCTGATATGGCATTATATTTATTTG
CAGATGGTATTACAAAACAAACTCCCATCAATGTTTTTAATAATGGAAATATGGCTAGAGATTTTACTTATGTCGATGAT
GTTGTAGATGGTGTTTATAGTGTACTTAAAAAGCCAGCTGAGAGTGATCGTAATTTTAATACACAAAATCCCAATTCCTC
AAGCTCGCTTGCTCCTTATAAGATATACAATATAGGTACTGGACATGCTATTAAGCTTATGGAGTTTATTGATGAGCTTG
AAACAAATCTTGGAAATAAAGCTTGTAAAAATTTTTTGCCCATGCAAAAAGCAGATGTTGTGGAGAGTTGCTGTAGTATT
TCAAAACTTAAGAATGATTTTGCTTATGAGGCCTTAACCTCTATTAAAGAGGGGATAAAAAAATTCGCACATTGGTATAA
ATCTAAGATAGTTAAGTAG

Upstream 100 bases:

>100_bases
AAGCTATAACAACTGATATTTAATTAGTTGGTGCTTTATGGTTATAGGGATTTAAGATAGTCAATTTATTGTTTATGTAG
TAATTGATGGAAGTTGTTGT

Downstream 100 bases:

>100_bases
TGTATTGTTGTGTATCTGTGGCATGGAATTATTGTATTTGTTGTTTTTTTAAGATTTTGTTTTTTAAAAGTAGAGTCAAT
TTTGGGTTTTAAGGTATATA

Product: UDP-glucuronate 4-epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRVFLTGIAGFIGFHVAKRLADNGHEVLGVDVINDYYEPNLKYERLKALGFDRENICGKKILQSNKYDNLSFICLDILNK
EQVLSLFSRYKFTHVCHLAAQAGIRDSIENPDSYISVNIVGFFNVLDACRIHKSHIEHFVYASTSAVYGINEKMPSDEDS
ITDHPLNLYAASKKSNEVIAHAYSSSFNIPTTGLRFFTVYGPYGRPDMALYLFADGITKQTPINVFNNGNMARDFTYVDD
VVDGVYSVLKKPAESDRNFNTQNPNSSSSLAPYKIYNIGTGHAIKLMEFIDELETNLGNKACKNFLPMQKADVVESCCSI
SKLKNDFAYEALTSIKEGIKKFAHWYKSKIVK

Sequences:

>Translated_352_residues
MRVFLTGIAGFIGFHVAKRLADNGHEVLGVDVINDYYEPNLKYERLKALGFDRENICGKKILQSNKYDNLSFICLDILNK
EQVLSLFSRYKFTHVCHLAAQAGIRDSIENPDSYISVNIVGFFNVLDACRIHKSHIEHFVYASTSAVYGINEKMPSDEDS
ITDHPLNLYAASKKSNEVIAHAYSSSFNIPTTGLRFFTVYGPYGRPDMALYLFADGITKQTPINVFNNGNMARDFTYVDD
VVDGVYSVLKKPAESDRNFNTQNPNSSSSLAPYKIYNIGTGHAIKLMEFIDELETNLGNKACKNFLPMQKADVVESCCSI
SKLKNDFAYEALTSIKEGIKKFAHWYKSKIVK
>Mature_352_residues
MRVFLTGIAGFIGFHVAKRLADNGHEVLGVDVINDYYEPNLKYERLKALGFDRENICGKKILQSNKYDNLSFICLDILNK
EQVLSLFSRYKFTHVCHLAAQAGIRDSIENPDSYISVNIVGFFNVLDACRIHKSHIEHFVYASTSAVYGINEKMPSDEDS
ITDHPLNLYAASKKSNEVIAHAYSSSFNIPTTGLRFFTVYGPYGRPDMALYLFADGITKQTPINVFNNGNMARDFTYVDD
VVDGVYSVLKKPAESDRNFNTQNPNSSSSLAPYKIYNIGTGHAIKLMEFIDELETNLGNKACKNFLPMQKADVVESCCSI
SKLKNDFAYEALTSIKEGIKKFAHWYKSKIVK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=366, Percent_Identity=26.775956284153, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=365, Percent_Identity=23.2876712328767, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI56118217, Length=365, Percent_Identity=23.2876712328767, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI189083684, Length=365, Percent_Identity=23.2876712328767, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI42516563, Length=358, Percent_Identity=23.7430167597765, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1786974, Length=363, Percent_Identity=22.3140495867769, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788353, Length=268, Percent_Identity=26.865671641791, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=23.2044198895028, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=370, Percent_Identity=24.3243243243243, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=24.5231607629428, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=359, Percent_Identity=24.7910863509749, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=350, Percent_Identity=22.8571428571429, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=23.8227146814404, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=258, Percent_Identity=29.0697674418605, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 39624; Mature: 39624

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFLTGIAGFIGFHVAKRLADNGHEVLGVDVINDYYEPNLKYERLKALGFDRENICGKK
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
ILQSNKYDNLSFICLDILNKEQVLSLFSRYKFTHVCHLAAQAGIRDSIENPDSYISVNIV
HHHCCCCCCCEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEH
GFFNVLDACRIHKSHIEHFVYASTSAVYGINEKMPSDEDSITDHPLNLYAASKKSNEVIA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEE
HAYSSSFNIPTTGLRFFTVYGPYGRPDMALYLFADGITKQTPINVFNNGNMARDFTYVDD
EECCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHH
VVDGVYSVLKKPAESDRNFNTQNPNSSSSLAPYKIYNIGTGHAIKLMEFIDELETNLGNK
HHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
ACKNFLPMQKADVVESCCSISKLKNDFAYEALTSIKEGIKKFAHWYKSKIVK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVFLTGIAGFIGFHVAKRLADNGHEVLGVDVINDYYEPNLKYERLKALGFDRENICGKK
CEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
ILQSNKYDNLSFICLDILNKEQVLSLFSRYKFTHVCHLAAQAGIRDSIENPDSYISVNIV
HHHCCCCCCCEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEH
GFFNVLDACRIHKSHIEHFVYASTSAVYGINEKMPSDEDSITDHPLNLYAASKKSNEVIA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCEEE
HAYSSSFNIPTTGLRFFTVYGPYGRPDMALYLFADGITKQTPINVFNNGNMARDFTYVDD
EECCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHH
VVDGVYSVLKKPAESDRNFNTQNPNSSSSLAPYKIYNIGTGHAIKLMEFIDELETNLGNK
HHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
ACKNFLPMQKADVVESCCSISKLKNDFAYEALTSIKEGIKKFAHWYKSKIVK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]