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The map label for this gene is metK [H]

Identifier: 187918243

GI number: 187918243

Start: 388442

End: 389611

Strand: Direct

Name: metK [H]

Synonym: BH0376

Alternate gene names: 187918243

Gene position: 388442-389611 (Clockwise)

Preceding gene: 187918242

Following gene: 187918244

Centisome position: 42.12

GC content: 32.74

Gene sequence:

>1170_bases
ATGCTCAATAATAATCTAACATCAACATCTGAAGCCGTATCTGAAGGACATCCGGATAAGATTGCGGATCAAATTTCTGA
CGCTATACTTGATGAAATACTTAAAAGAGATAAAATGGCAAAAGTCGCATGCGAGACTTTAGTTTCACAGAACTTAGTAG
TAATAGCAGGAGAGATAAACAGCAAAGCAAAAAAAGATATAGATATAAAGGAAATTGCAAAACAAACAATAAAAAATATT
GGATACACAAATATAGAATATGGACTTGACTATAAATCTGCTACAATAATTGATGCTATTGGCTCCCAGTCAATTGACAT
TACAAATGCAGTTGATAAAAAAGATACGAAAGATATAGGAGCAGGTGATCAGGGAATAATATTTGGTTATGCATGCAATG
AGACTGAAAATTTTTTACCAATAGCATATGAATTATCTAACTTAATCTTGCAAAAAGCCAGCGAACTTAGAAAATCAGGT
GAAATTAAATGGCTACGTCCTGACGCAAAATCCCAAGTTACCATTGAATATGATTCTAATAAAAATCCTATTAGAATCAA
TAATATTGTTGTATCTCATCAACATGACCCTGATATTCCCCAAGATTTCCTAAGACAAACAATTATTGAAAAGATTATAA
AACCTACCCTGCAAAGCAAATCAATGCTTGATGAGAATATCAAATATTATATTAACCCCTCAGGCAATTTTGTAATCGGA
GGACCTACTGGGGATACAGGTCTTACAGGAAGAAAGATTATTGCTGATAGTTATGGTGGATTTGCAAGGCATGGTGGTGG
TGCTTACAGCGGCAAAGATGCTACTAAGGTTGACCGGTCAGCTGCTTACATATCAAGATACATTGCTAAAAACATGGTAG
CCGCAGGCATTTCTAAAGAATTTGAAATGCAACTTGCTTATGCCATTGGAATTCCCAATCCAGTATCTATTAAAATAACT
ACAGGCATAAATGATCATGAATATGAAAAAAAAATATTAAGTTTTATCATAAATAACTTTGACCTAACTCCCAACGGTAT
AATTAAAAAATTAAAATTAAGAGAACCTATTTACTCTAAGACATGCATATACGGACATTTCGGAAAAAATGAACTAGAAT
GGGAAAAATTAGATTTCGTAGACATAATAAAAAAGGAGTTTAAAATATGA

Upstream 100 bases:

>100_bases
CATAGATTTCAATAAGTTCTTACAAGATGCATCAATGAACTCAGCCAAGATGGTAAGAGAACTCATTAAACTCATATAAT
TTATATACAAAGGTGATAAT

Downstream 100 bases:

>100_bases
ATAAAATATCGAGCTTTACAATTGATCACACAAAACTAAAACCTGGTATATATATATCAAGAAAAGATATATTTGAAAAT
TTAACATGCACTACTGTAGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLNNNLTSTSEAVSEGHPDKIADQISDAILDEILKRDKMAKVACETLVSQNLVVIAGEINSKAKKDIDIKEIAKQTIKNI
GYTNIEYGLDYKSATIIDAIGSQSIDITNAVDKKDTKDIGAGDQGIIFGYACNETENFLPIAYELSNLILQKASELRKSG
EIKWLRPDAKSQVTIEYDSNKNPIRINNIVVSHQHDPDIPQDFLRQTIIEKIIKPTLQSKSMLDENIKYYINPSGNFVIG
GPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYISRYIAKNMVAAGISKEFEMQLAYAIGIPNPVSIKIT
TGINDHEYEKKILSFIINNFDLTPNGIIKKLKLREPIYSKTCIYGHFGKNELEWEKLDFVDIIKKEFKI

Sequences:

>Translated_389_residues
MLNNNLTSTSEAVSEGHPDKIADQISDAILDEILKRDKMAKVACETLVSQNLVVIAGEINSKAKKDIDIKEIAKQTIKNI
GYTNIEYGLDYKSATIIDAIGSQSIDITNAVDKKDTKDIGAGDQGIIFGYACNETENFLPIAYELSNLILQKASELRKSG
EIKWLRPDAKSQVTIEYDSNKNPIRINNIVVSHQHDPDIPQDFLRQTIIEKIIKPTLQSKSMLDENIKYYINPSGNFVIG
GPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYISRYIAKNMVAAGISKEFEMQLAYAIGIPNPVSIKIT
TGINDHEYEKKILSFIINNFDLTPNGIIKKLKLREPIYSKTCIYGHFGKNELEWEKLDFVDIIKKEFKI
>Mature_389_residues
MLNNNLTSTSEAVSEGHPDKIADQISDAILDEILKRDKMAKVACETLVSQNLVVIAGEINSKAKKDIDIKEIAKQTIKNI
GYTNIEYGLDYKSATIIDAIGSQSIDITNAVDKKDTKDIGAGDQGIIFGYACNETENFLPIAYELSNLILQKASELRKSG
EIKWLRPDAKSQVTIEYDSNKNPIRINNIVVSHQHDPDIPQDFLRQTIIEKIIKPTLQSKSMLDENIKYYINPSGNFVIG
GPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYISRYIAKNMVAAGISKEFEMQLAYAIGIPNPVSIKIT
TGINDHEYEKKILSFIINNFDLTPNGIIKKLKLREPIYSKTCIYGHFGKNELEWEKLDFVDIIKKEFKI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=371, Percent_Identity=47.7088948787062, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI4557737, Length=371, Percent_Identity=47.9784366576819, Blast_Score=355, Evalue=5e-98,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=50.2659574468085, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538498, Length=380, Percent_Identity=48.4210526315789, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17538494, Length=380, Percent_Identity=48.4210526315789, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI17551082, Length=383, Percent_Identity=48.0417754569191, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145633, Length=380, Percent_Identity=48.1578947368421, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565905, Length=380, Percent_Identity=44.4736842105263, Blast_Score=311, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=47.112462006079, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI25145635, Length=329, Percent_Identity=46.5045592705167, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=48, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=89, Percent_Identity=47.1910112359551, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323209, Length=372, Percent_Identity=49.1935483870968, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=48.4042553191489, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=48.4126984126984, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=48.4126984126984, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=47.8835978835979, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=47.554347826087, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=47.8632478632479, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43202; Mature: 43202

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNNLTSTSEAVSEGHPDKIADQISDAILDEILKRDKMAKVACETLVSQNLVVIAGEIN
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
SKAKKDIDIKEIAKQTIKNIGYTNIEYGLDYKSATIIDAIGSQSIDITNAVDKKDTKDIG
CCHHCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
AGDQGIIFGYACNETENFLPIAYELSNLILQKASELRKSGEIKWLRPDAKSQVTIEYDSN
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCC
KNPIRINNIVVSHQHDPDIPQDFLRQTIIEKIIKPTLQSKSMLDENIKYYINPSGNFVIG
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
GPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYISRYIAKNMVAAGISKE
CCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
FEMQLAYAIGIPNPVSIKITTGINDHEYEKKILSFIINNFDLTPNGIIKKLKLREPIYSK
HHEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
TCIYGHFGKNELEWEKLDFVDIIKKEFKI
EEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNNNLTSTSEAVSEGHPDKIADQISDAILDEILKRDKMAKVACETLVSQNLVVIAGEIN
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
SKAKKDIDIKEIAKQTIKNIGYTNIEYGLDYKSATIIDAIGSQSIDITNAVDKKDTKDIG
CCHHCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCC
AGDQGIIFGYACNETENFLPIAYELSNLILQKASELRKSGEIKWLRPDAKSQVTIEYDSN
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCC
KNPIRINNIVVSHQHDPDIPQDFLRQTIIEKIIKPTLQSKSMLDENIKYYINPSGNFVIG
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEE
GPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYISRYIAKNMVAAGISKE
CCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
FEMQLAYAIGIPNPVSIKITTGINDHEYEKKILSFIINNFDLTPNGIIKKLKLREPIYSK
HHEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
TCIYGHFGKNELEWEKLDFVDIIKKEFKI
EEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA