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The map label for this gene is wcaF [H]

Identifier: 187736570

GI number: 187736570

Start: 2543977

End: 2544516

Strand: Direct

Name: wcaF [H]

Synonym: Amuc_2092

Alternate gene names: 187736570

Gene position: 2543977-2544516 (Clockwise)

Preceding gene: 187736569

Following gene: 187736571

Centisome position: 95.49

GC content: 61.48

Gene sequence:

>540_bases
ATGGATCTTTCCCGTTACCAGGAACACTACTCCCTCAGCCACCGCCTCAAACGGTATGGCTGGTACATCGTCAACCGGAC
GCTCTTCCGGCTCATGGTCACCAACGCCATGAAAGTCCCCCGGAACCTGCTGCTCCGCCTGTTCGGGGCAAACATACCGC
TTGCCTCCCTCGTTTACCCCAGCAGCAATATCTGGGCGCCATGGAATCTCTCCGTAGGGGAATACGCCTGCATCGGCCCT
GATACAGAAATTTACAACAAGGCCCCCATCTCCATCGGAAACCACGCCGTCATTTCCCAGGGGGCCTTCCTCTGCACGGC
CTCCCATGACATCTCGGATCCCGCCCACGCCCTCATCTCCGCCCCCATTACTGTGGAAGACCAGGCATGGGTGGCGGCGC
AGGCCTTTGTGGGCATGGGGGTGACGGTTGGCCGGGGAGCCGTGGTAGGAGCCCGCAGCGCCGTATTCAGGGATGTGGCC
CCCTGGACGGTTGTGGGCGGGAACCCGGCCCGATTCATCAAACAACGCACGCTCCGCTAA

Upstream 100 bases:

>100_bases
GCCGCTGGCCGCCTTTTCCACCGGAATAAAAACAGACGCGGAACCAGCCGCCACACCCCGGCCATAACCCCTCTTTCACC
ACTCACCCCCATCCCCAGCC

Downstream 100 bases:

>100_bases
ACCATGCTTGACCTCTCCGTTATCATCCTCGCCAGAAATGAGGAACTCCATATCCGCCGCTGTCTGGAAAACATTCTTCC
CGTCGCTAAAGAAGTCTTTG

Product: transferase hexapeptide repeat containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MDLSRYQEHYSLSHRLKRYGWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYPSSNIWAPWNLSVGEYACIGP
DTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALISAPITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVA
PWTVVGGNPARFIKQRTLR

Sequences:

>Translated_179_residues
MDLSRYQEHYSLSHRLKRYGWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYPSSNIWAPWNLSVGEYACIGP
DTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALISAPITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVA
PWTVVGGNPARFIKQRTLR
>Mature_179_residues
MDLSRYQEHYSLSHRLKRYGWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYPSSNIWAPWNLSVGEYACIGP
DTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALISAPITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVA
PWTVVGGNPARFIKQRTLR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=178, Percent_Identity=38.7640449438202, Blast_Score=124, Evalue=3e-30,
Organism=Escherichia coli, GI1786664, Length=139, Percent_Identity=30.9352517985612, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 19696; Mature: 19696

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSRYQEHYSLSHRLKRYGWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SSNIWAPWNLSVGEYACIGPDTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALIS
CCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEECCCEEEEECCCCCCCHHHHHHC
APITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVAPWTVVGGNPARFIKQRTLR
CCEEECCCHHHHHHHHHHCCEEECCCCEECCHHHHHHCCCCEEEECCCHHHHHHHHCCC
>Mature Secondary Structure
MDLSRYQEHYSLSHRLKRYGWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
SSNIWAPWNLSVGEYACIGPDTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALIS
CCCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEECCCEEEEECCCCCCCHHHHHHC
APITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVAPWTVVGGNPARFIKQRTLR
CCEEECCCHHHHHHHHHHCCEEECCCCEECCHHHHHHCCCCEEEECCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]