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The map label for this gene is ruvC

Identifier: 187736503

GI number: 187736503

Start: 2457802

End: 2458305

Strand: Reverse

Name: ruvC

Synonym: Amuc_2024

Alternate gene names: 187736503

Gene position: 2458305-2457802 (Counterclockwise)

Preceding gene: 187736504

Following gene: 187736500

Centisome position: 92.28

GC content: 60.12

Gene sequence:

>504_bases
ATGCGCATTCTTGCCATAGACCCGGCCATCCGCAACACGGGTTATGCCGTGGTGGAGGGGGATTACCGGCGCGCGCGCGC
CCTGGACTATGGAACGCTATCCATTCCCCGGAGCGTGTCCCAGTCCGGCTGCCTGCTTGCCATCAAGCAGCATTTGGGCA
ATCTCATTGACAAATGGAACCCGGATGAAATGGCGGTGGAGCGCATCATCTATGTCCAGTCTCATCAGACGGCCATCACG
ATGGGTGCGGCCAAGGCGGCCGTGGTCATTGCCGCGGCGGAAGCCGGGCTGCGTATTATGGAATATTCCCCCAAAAGCGT
GAAGCTCTCCGTCGTAGGGCGCGGGGCCGCTCAAAAAACGCAGGTCGCCTTCATGGTGCGGGCTCTTTTGGAACTCCGGG
AAACGCCGGAATCCGATGCCGCCGACGCCCTGGCGATCGGCCTCACCCACCTCTTTTCTGCGGACCCTTTGAAGGCCCAC
ATGATGGAGAGGAAATACATTTAG

Upstream 100 bases:

>100_bases
ATCCGTCCGCAAGCCCAAACCCGCAGGTCCCAAGCGCCGCAGGCGCTAAGGAGGGGGGGGAAAACATGATGTTTCTCTCT
GATGGAATGAAGATCATCCC

Downstream 100 bases:

>100_bases
TAGTGATGAGATATGAGTGTAAAAACCGTACTTGCAGCCCGCGGATTGAAATCTGACCGCCCGGCGCTGTTCTCTAATCA
CTGATTCAAGCCGTCAGGGC

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWNPDEMAVERIIYVQSHQTAIT
MGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKTQVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAH
MMERKYI

Sequences:

>Translated_167_residues
MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWNPDEMAVERIIYVQSHQTAIT
MGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKTQVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAH
MMERKYI
>Mature_167_residues
MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWNPDEMAVERIIYVQSHQTAIT
MGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKTQVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAH
MMERKYI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=150, Percent_Identity=38.6666666666667, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_AKKM8 (B2UP63)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878615.1
- ProteinModelPortal:   B2UP63
- SMR:   B2UP63
- GeneID:   6274776
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_2024
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18192; Mature: 18192

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWN
CEEEEECCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDEMAVERIIYVQSHQTAITMGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKT
CHHHHHHHHHEEECCCEEEEECCCCEEEEEEECCCCEEEEEECCCEEEEEEEECCCCHHH
QVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAHMMERKYI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWN
CEEEEECCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PDEMAVERIIYVQSHQTAITMGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKT
CHHHHHHHHHEEECCCEEEEECCCCEEEEEEECCCCEEEEEECCCEEEEEEEECCCCHHH
QVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAHMMERKYI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA