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The map label for this gene is ppdK [H]

Identifier: 187736456

GI number: 187736456

Start: 2396408

End: 2399152

Strand: Reverse

Name: ppdK [H]

Synonym: Amuc_1974

Alternate gene names: 187736456

Gene position: 2399152-2396408 (Counterclockwise)

Preceding gene: 187736457

Following gene: 187736451

Centisome position: 90.05

GC content: 59.6

Gene sequence:

>2745_bases
ATGTCCACCAATTCCTGCGCGTGTAGCGCATCTACTGACAAATTCGTCTATTCTTTCGGCGGAGGGTCCGCCGACGGCAA
TGGCAGCATGAAAAATCTTTTGGGCGGCAAGGGCGCCAACCTTGCGGAAATGGCCCGTATCGGCCTTCCTGTCCCTCCCG
GATTCACCATCACTACGGAAGTTTGCACTTACTACTACGATCACGGCTGCACTTATCCCGCCCAGCTTGACGCGCAGGTC
AAGGAAGGCATTGCCAAAATGGAACAGATCCTGGGACGCAAGTTCGGCGATACGGAAGCTATGCCCCTGCTGGTGGCCGT
GCGTTCCGGCGCCCGTGAATCCATGCCCGGCATGATGGACACGATTTTGAACCTCGGCCTGAATGAAAAATCCGTGGAAG
CCATGGTGAAGGCTACGAACAACCCCCGCTTCGCCTGGGACTGCTACCGCCGCTTCGTGCAGATGTACGGCGACGTGGTG
CTGGGTGTGCAGAAAACCCCGGATGAAGACCACGAACCCTTTGAAACCGCCATCGCTGCCATCAAGAAAGAACGCTTTGG
CAGCGAGGACGTGGAAGACACCAAGCTCTCTGCCGATGACCTGAAGGAACTTGTCTCCCGCTTCAAGGCCCTGGTGCTGG
AACGCACCGGCCATGAATTCCCGGAATGCCCCTGGGAACAGCTTCAGGGAGCCATTGGCGCCGTGTTCGGTTCCTGGAAC
AATGACCGCGCCATCGTGTACCGCCAGAAATACGGCATTCCGTCCGAATGGGGCACCGCCGTCAACGTGCAGGCCATGGT
CTTCGGCAACACGGGTGAGGAATCCGGTTCCGGCGTGGCGTTCACCCGCAACCCCGCCTCCGGCGAAAACGAATTCTATG
GCGAATTCCTGATGAACGCCCAGGGTGAAGACGTCGTGGCCGGCGTGCGTACGCCCGCCCCCGTTGCTGCCCTTCATGAA
GTGATGCCCGCCGCCTTCAATGAACTGGTGCGCATTCGCGAAGTGCTGGAAAACCACTTCCATGACATGCAGGACTTTGA
ATTCACCATTCAGGACCGCACCGTCTACATGCTCCAGACCCGCAACGGAAAGCGCACCGGCGTGGCCGCTTTCCGCATTG
CCTGTGAAATGGTGGAACAGGGCCTGATTGACTGGAAAACCGCCGTGCGCCGCATTCCTGCGGACCAGGTGGACCAGCTG
CTGACCCCCATCTTTGACCGTGAAGCCATCAAGAGTGCCAAGGTTCTGACCCGCGGTCTTCCCGCCGGGCCCGGCGCCGC
CACCGGACGCATCTACCTGAACGCGGAACGCTGTGTGGAAGCCGCGGATAGAGGTGAAAAGGTTCTGCTGGTCCGTCTGG
AAACCTCTCCGGAAGACCTGCGCGGCATGATTGCCGCTGAAGGCATCCTGACCGCCCGCGGCGGCGTTTCCTCCCATGCC
GCCCTCGTTGCCCGCCAGATGGGCAAAGTCTGCGTTTGCGGCGCGGATGAAATCATTGTGGACTACAACAACCGCACCGT
TTCCGTTGGCGACGTAACCATCAGCGAAGGTGACTACCTCTCCATTGACGGAACGAGCGGCCTGGTATACGCCGGCAAGG
TGGAAACTTCCCCCTCTGAAATCATCCAGGTCCTGATTTCCAAGACCATGAAGCCGGAAGACAGCCGCACCTACCAGAAC
TTCGCCAAGCTCATGCAGTGGTGCGACGACTGTACCAAAATGAAAGTGCGCACCAATGCGGACTCTCCCAAGCAGACGGA
AACCGCCATCGCCTTCGGCGCCACCGGCATCGGCCTCTGCCGCACGGAACACATGTTCTTTGAAGGGGACCGGATCGACT
TCGTCCGTGAAATGATCCTCTCCACCAAGAAGAGCGACCGCGTAGCCGCCGTTTCCAAGCTCCTTCCCTACCAGAAGGGG
GACTTCAAGGGCATCTTCAAGGCGCTCAAGGGCCTGCCGGGCACCATCCGCCTTCTGGACCCGCCCCTGCACGAATTCCT
TCCCCAGACGAAGGAACAGCAGCTTGACCTGGCCCAGAAAATCGGCATGCCTGTGGAAGTTATCATGCGCCGCGTGTCCG
AACTCCATGAGTTCAACCCGATGCTCGGCCACCGCGGCTGCCGTCTCGGCATCGCCTATCCGGAAATCACGGAAATGCAG
GCCCGCGCCATCTTTGAAGCCGCTGTGGAAGTCTCCCAGGAAGAAGGATTTGCCGTGGTGCCTGAAATCATGGTTCCTCT
GGTGGCCTTCAAGAAGGAACTGGACATCCAGAAAGCCATCATTGACAAGGTGGCCCAGCAGGTATTTGAGGAAAAAGGCG
TCAAAGTGGACTACCTCGTCGGCACCATGATTGAAATCCCGCGCGCTGCCGTCACGGCTGACGAAATCGCGGAAACCGCC
CAGTTCTTCTCCTTCGGCACCAATGACCTGACCCAGACGGGCCTGGGCATGAGCCGCGACGACTCCGGCTCCTTCCTGCC
GCAGTACCAGGAACTGGAAATCATCAAGAAGAACGTCTTCGCTTCCATTGACCAGAACGGCGTCGGCAAGCTGATGAAGA
TTGCCGTGGAACTCGGCCGCTCCACCCGCCCGGACATCAAACTGGGCATCTGCGGCGAACACGGCGGCGACCCCGCTTCC
GTCAAGTTCTGCAACACGCTGGGCCTGACTTATGTGAGCTGCTCGCCCTACCGCGTCCCGACCGCCCGTCTGGCCGCGGC
CCAGGCTGCTCTGGAACAGGAATAA

Upstream 100 bases:

>100_bases
CGAAGGCATCCTTATTCGACCCTAACTCGCTATTGGGTCTTTTGTTGGCTCCTTTGGTCGATGTTTGGGCAGAATCATTA
ATTCGACTACAATAATCGAT

Downstream 100 bases:

>100_bases
ACCTTTTTGTTAAGGGTTAATAAACAGCCCGTGGTTCTTCGGAACCACGGGCTGTTTTAGCGGATGGAACGGTCTTGTCA
GATATGGCTTGAACCTTGCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 914; Mature: 913

Protein sequence:

>914_residues
MSTNSCACSASTDKFVYSFGGGSADGNGSMKNLLGGKGANLAEMARIGLPVPPGFTITTEVCTYYYDHGCTYPAQLDAQV
KEGIAKMEQILGRKFGDTEAMPLLVAVRSGARESMPGMMDTILNLGLNEKSVEAMVKATNNPRFAWDCYRRFVQMYGDVV
LGVQKTPDEDHEPFETAIAAIKKERFGSEDVEDTKLSADDLKELVSRFKALVLERTGHEFPECPWEQLQGAIGAVFGSWN
NDRAIVYRQKYGIPSEWGTAVNVQAMVFGNTGEESGSGVAFTRNPASGENEFYGEFLMNAQGEDVVAGVRTPAPVAALHE
VMPAAFNELVRIREVLENHFHDMQDFEFTIQDRTVYMLQTRNGKRTGVAAFRIACEMVEQGLIDWKTAVRRIPADQVDQL
LTPIFDREAIKSAKVLTRGLPAGPGAATGRIYLNAERCVEAADRGEKVLLVRLETSPEDLRGMIAAEGILTARGGVSSHA
ALVARQMGKVCVCGADEIIVDYNNRTVSVGDVTISEGDYLSIDGTSGLVYAGKVETSPSEIIQVLISKTMKPEDSRTYQN
FAKLMQWCDDCTKMKVRTNADSPKQTETAIAFGATGIGLCRTEHMFFEGDRIDFVREMILSTKKSDRVAAVSKLLPYQKG
DFKGIFKALKGLPGTIRLLDPPLHEFLPQTKEQQLDLAQKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQ
ARAIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGVKVDYLVGTMIEIPRAAVTADEIAETA
QFFSFGTNDLTQTGLGMSRDDSGSFLPQYQELEIIKKNVFASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPAS
VKFCNTLGLTYVSCSPYRVPTARLAAAQAALEQE

Sequences:

>Translated_914_residues
MSTNSCACSASTDKFVYSFGGGSADGNGSMKNLLGGKGANLAEMARIGLPVPPGFTITTEVCTYYYDHGCTYPAQLDAQV
KEGIAKMEQILGRKFGDTEAMPLLVAVRSGARESMPGMMDTILNLGLNEKSVEAMVKATNNPRFAWDCYRRFVQMYGDVV
LGVQKTPDEDHEPFETAIAAIKKERFGSEDVEDTKLSADDLKELVSRFKALVLERTGHEFPECPWEQLQGAIGAVFGSWN
NDRAIVYRQKYGIPSEWGTAVNVQAMVFGNTGEESGSGVAFTRNPASGENEFYGEFLMNAQGEDVVAGVRTPAPVAALHE
VMPAAFNELVRIREVLENHFHDMQDFEFTIQDRTVYMLQTRNGKRTGVAAFRIACEMVEQGLIDWKTAVRRIPADQVDQL
LTPIFDREAIKSAKVLTRGLPAGPGAATGRIYLNAERCVEAADRGEKVLLVRLETSPEDLRGMIAAEGILTARGGVSSHA
ALVARQMGKVCVCGADEIIVDYNNRTVSVGDVTISEGDYLSIDGTSGLVYAGKVETSPSEIIQVLISKTMKPEDSRTYQN
FAKLMQWCDDCTKMKVRTNADSPKQTETAIAFGATGIGLCRTEHMFFEGDRIDFVREMILSTKKSDRVAAVSKLLPYQKG
DFKGIFKALKGLPGTIRLLDPPLHEFLPQTKEQQLDLAQKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQ
ARAIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGVKVDYLVGTMIEIPRAAVTADEIAETA
QFFSFGTNDLTQTGLGMSRDDSGSFLPQYQELEIIKKNVFASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPAS
VKFCNTLGLTYVSCSPYRVPTARLAAAQAALEQE
>Mature_913_residues
STNSCACSASTDKFVYSFGGGSADGNGSMKNLLGGKGANLAEMARIGLPVPPGFTITTEVCTYYYDHGCTYPAQLDAQVK
EGIAKMEQILGRKFGDTEAMPLLVAVRSGARESMPGMMDTILNLGLNEKSVEAMVKATNNPRFAWDCYRRFVQMYGDVVL
GVQKTPDEDHEPFETAIAAIKKERFGSEDVEDTKLSADDLKELVSRFKALVLERTGHEFPECPWEQLQGAIGAVFGSWNN
DRAIVYRQKYGIPSEWGTAVNVQAMVFGNTGEESGSGVAFTRNPASGENEFYGEFLMNAQGEDVVAGVRTPAPVAALHEV
MPAAFNELVRIREVLENHFHDMQDFEFTIQDRTVYMLQTRNGKRTGVAAFRIACEMVEQGLIDWKTAVRRIPADQVDQLL
TPIFDREAIKSAKVLTRGLPAGPGAATGRIYLNAERCVEAADRGEKVLLVRLETSPEDLRGMIAAEGILTARGGVSSHAA
LVARQMGKVCVCGADEIIVDYNNRTVSVGDVTISEGDYLSIDGTSGLVYAGKVETSPSEIIQVLISKTMKPEDSRTYQNF
AKLMQWCDDCTKMKVRTNADSPKQTETAIAFGATGIGLCRTEHMFFEGDRIDFVREMILSTKKSDRVAAVSKLLPYQKGD
FKGIFKALKGLPGTIRLLDPPLHEFLPQTKEQQLDLAQKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQA
RAIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGVKVDYLVGTMIEIPRAAVTADEIAETAQ
FFSFGTNDLTQTGLGMSRDDSGSFLPQYQELEIIKKNVFASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPASV
KFCNTLGLTYVSCSPYRVPTARLAAAQAALEQE

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=441, Percent_Identity=26.0770975056689, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1788756, Length=465, Percent_Identity=25.1612903225806, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 100127; Mature: 99995

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNSCACSASTDKFVYSFGGGSADGNGSMKNLLGGKGANLAEMARIGLPVPPGFTITTE
CCCCCCEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCEEHHH
VCTYYYDHGCTYPAQLDAQVKEGIAKMEQILGRKFGDTEAMPLLVAVRSGARESMPGMMD
HHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCHHCCCHHHH
TILNLGLNEKSVEAMVKATNNPRFAWDCYRRFVQMYGDVVLGVQKTPDEDHEPFETAIAA
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHH
IKKERFGSEDVEDTKLSADDLKELVSRFKALVLERTGHEFPECPWEQLQGAIGAVFGSWN
HHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
NDRAIVYRQKYGIPSEWGTAVNVQAMVFGNTGEESGSGVAFTRNPASGENEFYGEFLMNA
CCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCC
QGEDVVAGVRTPAPVAALHEVMPAAFNELVRIREVLENHFHDMQDFEFTIQDRTVYMLQT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEE
RNGKRTGVAAFRIACEMVEQGLIDWKTAVRRIPADQVDQLLTPIFDREAIKSAKVLTRGL
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAGPGAATGRIYLNAERCVEAADRGEKVLLVRLETSPEDLRGMIAAEGILTARGGVSSHA
CCCCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCEEEECCCCCHHH
ALVARQMGKVCVCGADEIIVDYNNRTVSVGDVTISEGDYLSIDGTSGLVYAGKVETSPSE
HHHHHHHCCEEEECCCEEEEEECCCEEEECEEEECCCCEEEEECCCCEEEEEECCCCHHH
IIQVLISKTMKPEDSRTYQNFAKLMQWCDDCTKMKVRTNADSPKQTETAIAFGATGIGLC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHEEEECCCCCCHH
RTEHMFFEGDRIDFVREMILSTKKSDRVAAVSKLLPYQKGDFKGIFKALKGLPGTIRLLD
EECCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECC
PPLHEFLPQTKEQQLDLAQKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQ
CCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEECCCCCHHHHH
ARAIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGVKVDYLV
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEH
GTMIEIPRAAVTADEIAETAQFFSFGTNDLTQTGLGMSRDDSGSFLPQYQELEIIKKNVF
HHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
ASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPASVKFCNTLGLTYVSCSPYRVP
HHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEHHCCCEEEECCCCCCC
TARLAAAQAALEQE
HHHHHHHHHHHCCC
>Mature Secondary Structure 
STNSCACSASTDKFVYSFGGGSADGNGSMKNLLGGKGANLAEMARIGLPVPPGFTITTE
CCCCCEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCEEHHH
VCTYYYDHGCTYPAQLDAQVKEGIAKMEQILGRKFGDTEAMPLLVAVRSGARESMPGMMD
HHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCHHCCCHHHH
TILNLGLNEKSVEAMVKATNNPRFAWDCYRRFVQMYGDVVLGVQKTPDEDHEPFETAIAA
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHH
IKKERFGSEDVEDTKLSADDLKELVSRFKALVLERTGHEFPECPWEQLQGAIGAVFGSWN
HHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
NDRAIVYRQKYGIPSEWGTAVNVQAMVFGNTGEESGSGVAFTRNPASGENEFYGEFLMNA
CCCEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCC
QGEDVVAGVRTPAPVAALHEVMPAAFNELVRIREVLENHFHDMQDFEFTIQDRTVYMLQT
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEE
RNGKRTGVAAFRIACEMVEQGLIDWKTAVRRIPADQVDQLLTPIFDREAIKSAKVLTRGL
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAGPGAATGRIYLNAERCVEAADRGEKVLLVRLETSPEDLRGMIAAEGILTARGGVSSHA
CCCCCCCCCEEEEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCEEEECCCCCHHH
ALVARQMGKVCVCGADEIIVDYNNRTVSVGDVTISEGDYLSIDGTSGLVYAGKVETSPSE
HHHHHHHCCEEEECCCEEEEEECCCEEEECEEEECCCCEEEEECCCCEEEEEECCCCHHH
IIQVLISKTMKPEDSRTYQNFAKLMQWCDDCTKMKVRTNADSPKQTETAIAFGATGIGLC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCHHHHEEEECCCCCCHH
RTEHMFFEGDRIDFVREMILSTKKSDRVAAVSKLLPYQKGDFKGIFKALKGLPGTIRLLD
EECCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECC
PPLHEFLPQTKEQQLDLAQKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQ
CCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHCCCCCEEECCCCCHHHHH
ARAIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGVKVDYLV
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEH
GTMIEIPRAAVTADEIAETAQFFSFGTNDLTQTGLGMSRDDSGSFLPQYQELEIIKKNVF
HHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
ASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPASVKFCNTLGLTYVSCSPYRVP
HHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEHHCCCEEEECCCCCCC
TARLAAAQAALEQE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]