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The map label for this gene is pat [H]

Identifier: 187736417

GI number: 187736417

Start: 2348584

End: 2349183

Strand: Reverse

Name: pat [H]

Synonym: Amuc_1935

Alternate gene names: 187736417

Gene position: 2349183-2348584 (Counterclockwise)

Preceding gene: 187736418

Following gene: 187736416

Centisome position: 88.18

GC content: 48.33

Gene sequence:

>600_bases
ATGAAAAGCAGCAGAATCAGAATTGCAACGAGGCGGGATGCGGCCAAGCTGCTGGAAATTTACGCTCCGTATGTGGAAAA
GACAGCTGTCACGTTTGAATATGAAGTGCCTGCAGTGACGGAGTTTGAAAAAAGAATAGATCACGTACTGGGAAAATACC
CCTATTTGGTTGCGGAAAGGGCAGGGGATATTTCCGGTTATGCCTATGCAGGAGTGTTCAAGGACCGCGCAGCGTATCGC
TGGGCTGTGGAAACGACGGTATACGTGCGGGAAGACATGAAAAAAATGGGAACGGGAAGGGAACTGTATGCAGCTCTGGA
AAAGATTCTCTCCATGCAGAATATTTTGAATTTAAACGCGTGCATTGCTTATCCCTGTGAAGCTGAGGACCCCTATCTTA
CACGGGACAGCGTCCAGTTCCATAAACGTTTGGGGTATCAATTTGCAGGGAAATTTCATCGGTGCGGATACAAATTCGGC
CGTTGGTACCATATGATCTGGATGGAGAAGTTTCTCGGAGGCCATACGGAAAATCCGCCCGCAGTGGAATCTTTTGGCGA
GGTAAGGAGCCTTGTCGCGGAGAAATTCGGAATTTGGTGA

Upstream 100 bases:

>100_bases
AATGGCGGGTACTTGGGCGGCCTCAAATTGACAATCGCTTGTGAAATATTAGAATGATCGGCAGCGCTTCCGGCTCAGGG
GCCCAGCACGGTCAACCGAA

Downstream 100 bases:

>100_bases
AAGGCGGCTCCTGGGCGCATTCGGAAACAGAAAATATTTCCGAATCAATAACATGCCGATGTTCCGGCATGCGGCCACAA
GAGGGGTTTTTTGTTGCAAA

Product: GCN5-related N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKSSRIRIATRRDAAKLLEIYAPYVEKTAVTFEYEVPAVTEFEKRIDHVLGKYPYLVAERAGDISGYAYAGVFKDRAAYR
WAVETTVYVREDMKKMGTGRELYAALEKILSMQNILNLNACIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFG
RWYHMIWMEKFLGGHTENPPAVESFGEVRSLVAEKFGIW

Sequences:

>Translated_199_residues
MKSSRIRIATRRDAAKLLEIYAPYVEKTAVTFEYEVPAVTEFEKRIDHVLGKYPYLVAERAGDISGYAYAGVFKDRAAYR
WAVETTVYVREDMKKMGTGRELYAALEKILSMQNILNLNACIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFG
RWYHMIWMEKFLGGHTENPPAVESFGEVRSLVAEKFGIW
>Mature_199_residues
MKSSRIRIATRRDAAKLLEIYAPYVEKTAVTFEYEVPAVTEFEKRIDHVLGKYPYLVAERAGDISGYAYAGVFKDRAAYR
WAVETTVYVREDMKKMGTGRELYAALEKILSMQNILNLNACIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFG
RWYHMIWMEKFLGGHTENPPAVESFGEVRSLVAEKFGIW

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=181, Percent_Identity=30.939226519337, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 23054; Mature: 23054

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSRIRIATRRDAAKLLEIYAPYVEKTAVTFEYEVPAVTEFEKRIDHVLGKYPYLVAER
CCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHC
AGDISGYAYAGVFKDRAAYRWAVETTVYVREDMKKMGTGRELYAALEKILSMQNILNLNA
CCCCCCEEEHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
CIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFGRWYHMIWMEKFLGGHTENPP
EEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
AVESFGEVRSLVAEKFGIW
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSSRIRIATRRDAAKLLEIYAPYVEKTAVTFEYEVPAVTEFEKRIDHVLGKYPYLVAER
CCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHC
AGDISGYAYAGVFKDRAAYRWAVETTVYVREDMKKMGTGRELYAALEKILSMQNILNLNA
CCCCCCEEEHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
CIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFGRWYHMIWMEKFLGGHTENPP
EEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCH
AVESFGEVRSLVAEKFGIW
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3240868; 8593056 [H]