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The map label for this gene is hsdM [H]

Identifier: 187736397

GI number: 187736397

Start: 2324114

End: 2326675

Strand: Reverse

Name: hsdM [H]

Synonym: Amuc_1915

Alternate gene names: 187736397

Gene position: 2326675-2324114 (Counterclockwise)

Preceding gene: 187736398

Following gene: 187736396

Centisome position: 87.33

GC content: 51.48

Gene sequence:

>2562_bases
ATGAACAAACAACAGCTTGCATCGAAGATTTGGGAGTCTGCCAACAAAATGCGCTCCAAAATTGAAGCCAATGAATACAA
GGACTACATTCTCGGCTTCATCTTCTACAAGTTCCTTTCCGACAAGGAAGTCAAGTTCCTGAAAGAGAACGACTGGACAG
ACGATGATTTGCCCCATGTTACGGAAGATGACGCGGAGACGGTTGACTATATCCGCAGCAATGTAGGTTACTTCATCTCC
TATAAACACCTCTTTTCTACATGGATTGACAAGGGCAGCGACTTCAACGCCGCTGATGTGACGGAGGCTCTTTCTGCTTT
CAGCCGCCTGATCAATCCCTCTCACAAAAAGGTCTTTGACAAGGTGTTCGCCACCTTGGAAACAGGCCTCTCCAAACTTG
GGGAAAACTCCGGTGCCCGCACCAAAGCCATCCGCGACCTCTTGCATCTCATCAAGGATATCCCCATGGATGGGCGGCAG
GATTATGATGTCCTGGGGTTTATCTACGAATACCTCATCAGCAACTTTGCTGCAAACGCGGGCAAGAAAGCGGGCGAATT
CTACACCCCGCATGAGGTTTCGCTCCTCATGTCGGAAATCGTGGCTGCCCACCTTAAAGACAGGCAGCAAATCAAGATTT
ATGACCCCACCAGCGGTTCAGGCTCCCTGCTTATCAATATCGGCAAGTGCGTGGCGCGTTACATGGGCGGGGGAGACAAC
ATCAAATACTACGCTCAGGAGCTTAAGGAAAACACCTACAACCTGACCCGCATGAATCTGGTAATGCGGGGGATTTTGCC
GAACAATATCGTGACGCGCAACGGCGACACGCTGGAGGAAGATTGGCCGTATTTTGATGATAACGACCCGGTTAACACCT
ATGACCCGCTCTATGTAGATGCTGTTGTCTCCAACCCTCCCTATTCCCAATCGTGGAATCCCGCAGACAAGGAAAGCGAC
CCGCGTTACCGCTTTGGGCTTGCCCCGAAAGGCAAGGCTGACTATGCATTCCTGCTGCATGACCTATACCACCTCAAGCC
TGACGGCATCATGACGATTGTACTGCCCCATGGCGTCCTGTTCCGTGGCGGTACGGAGGGGGCTATCCGCAGGAATCTGG
TGGAATACAACCATATTGACGCTATCATCGGCTTGCCTGCCAACATTTTCTTTGGCACCGGTATTCCCACCATCATTATG
GTGCTCAAGCAGAAACGGGAAAATACGGATGTGCTGATTGTGGATGCGTCCAAGGGCTTTGCCAAGGTGGGCAAGAACAA
TGTCCTCCGCGCGTGCGACATCAAGAAGATTGTGGATGTGGTTTCTGCCCGCGCCGATGTGGAGAAGTTCGCCAAGGTGG
TAAGCCTTGATGAAATCCGGGGCAATGACTATAACCTCAATATACCGCGCTATGTCGATTCCTCAGACAAAAACGAACGT
TGGGATATTTTCGCTACCATGTTTGGCGGCATCCCCTTGTCTGAACTGGCGGATTTTCATGAGTACTGGGCAGCATTCCC
TCATCTGAAAGATGCTCTGTTCACCGCTTCCGGCGAGGCTCATTGCCGCTTGAACGTGGCTAACCTCAAGTCGTCCGTTC
TTGACGCCGCTGATGTTACGGCATTTACCGCTCGCTACGCTTCCTCTTTTGCTGATTTTGAAGCATTCCTCACCACCCGA
TTGCTCGATGGTATGGAAACGCTCAATATCACCAGAGAAAAATCCATCATCAGTGATGATGTTTTCTCTCGCCTGAACGG
GCTTCCCCTGATAGACAAATACAAAGCCTACCAGTTGCTGGATGATGAGTGGAAAAAGATTGCCGTGGACCTGGAGATTA
TCAGGACTGAGGGCTTTGCTTCTTCCAAACAGGTTGACCCCAACATGGTCGTTAAAACCAAGGACGGCAAAAAGCAGGAG
GTGCAGGACGGCTGGATTGGACACATTATTCCCTTTGCCCTGGTGCAGCAAACCCTGCTCGCCGCAGACCTAGCCAGGCT
GAACGCCAGGGAAGCCCGCTTGAGTGAACTCGCGGCAGAGTATGAATCCTTGCTGAATGAGCTGAGCGAGGAGGATAAGG
ACAAGCCCTTTGTCAATGATGAGGGCGACGCCTTTGTGCCGAAAGAGGTTGCAAAGGCCATCAAGAGCATGGAGGTTGAG
CCGGAAACGCTCGCCGTCCTGCGAAAGGTGAACGCCCTGAATAATGAGGAAAAATCCCTCAAGAAACAAATCAAGGCGGA
CAGTACCGCGCTCCACCTCCTCACCAAAAAGACCATTGAGGAATTGACGGATGAGCAGGCAAGGCAAATGATTAAGCTCA
AGTGGATTCCTCCCGTGCTGGATAGCATGGCCGGGTTGCCCTCCGCTGTCATTGCCGATTTCATTGCCCGACTCCTTGCT
TTGGCTGGGAAATATGAAACGACATTCACGGAGGTAGACAGCCAAATCGCTGAGACAGAGTCCTCTCTCGTATCGCTTTT
GGATGAATTGACCGGCAACGAGTTCGATATGCAGGGCATTCAGGAACTCAAATCCCTGCTCGGAGGAAGCGCCCATGAAT
GA

Upstream 100 bases:

>100_bases
GAATGAGGAAAGACCACGCTGCAAGCGGAAAAAATGGAATTGCTGATGGGCCTGTCGCTGGACTTTTGGGAAGATATGTG
TATACTGTTCAACCCTGTCT

Downstream 100 bases:

>100_bases
GAAATCGTTGATTCCGTCTATCCGCTTTGCCGGATTTACTGACGCATGGGAACGGCGTAAGCTGGGGGATTTAGCGGAGT
TTAGAAGAGGGCTAACCTAT

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 853; Mature: 853

Protein sequence:

>853_residues
MNKQQLASKIWESANKMRSKIEANEYKDYILGFIFYKFLSDKEVKFLKENDWTDDDLPHVTEDDAETVDYIRSNVGYFIS
YKHLFSTWIDKGSDFNAADVTEALSAFSRLINPSHKKVFDKVFATLETGLSKLGENSGARTKAIRDLLHLIKDIPMDGRQ
DYDVLGFIYEYLISNFAANAGKKAGEFYTPHEVSLLMSEIVAAHLKDRQQIKIYDPTSGSGSLLINIGKCVARYMGGGDN
IKYYAQELKENTYNLTRMNLVMRGILPNNIVTRNGDTLEEDWPYFDDNDPVNTYDPLYVDAVVSNPPYSQSWNPADKESD
PRYRFGLAPKGKADYAFLLHDLYHLKPDGIMTIVLPHGVLFRGGTEGAIRRNLVEYNHIDAIIGLPANIFFGTGIPTIIM
VLKQKRENTDVLIVDASKGFAKVGKNNVLRACDIKKIVDVVSARADVEKFAKVVSLDEIRGNDYNLNIPRYVDSSDKNER
WDIFATMFGGIPLSELADFHEYWAAFPHLKDALFTASGEAHCRLNVANLKSSVLDAADVTAFTARYASSFADFEAFLTTR
LLDGMETLNITREKSIISDDVFSRLNGLPLIDKYKAYQLLDDEWKKIAVDLEIIRTEGFASSKQVDPNMVVKTKDGKKQE
VQDGWIGHIIPFALVQQTLLAADLARLNAREARLSELAAEYESLLNELSEEDKDKPFVNDEGDAFVPKEVAKAIKSMEVE
PETLAVLRKVNALNNEEKSLKKQIKADSTALHLLTKKTIEELTDEQARQMIKLKWIPPVLDSMAGLPSAVIADFIARLLA
LAGKYETTFTEVDSQIAETESSLVSLLDELTGNEFDMQGIQELKSLLGGSAHE

Sequences:

>Translated_853_residues
MNKQQLASKIWESANKMRSKIEANEYKDYILGFIFYKFLSDKEVKFLKENDWTDDDLPHVTEDDAETVDYIRSNVGYFIS
YKHLFSTWIDKGSDFNAADVTEALSAFSRLINPSHKKVFDKVFATLETGLSKLGENSGARTKAIRDLLHLIKDIPMDGRQ
DYDVLGFIYEYLISNFAANAGKKAGEFYTPHEVSLLMSEIVAAHLKDRQQIKIYDPTSGSGSLLINIGKCVARYMGGGDN
IKYYAQELKENTYNLTRMNLVMRGILPNNIVTRNGDTLEEDWPYFDDNDPVNTYDPLYVDAVVSNPPYSQSWNPADKESD
PRYRFGLAPKGKADYAFLLHDLYHLKPDGIMTIVLPHGVLFRGGTEGAIRRNLVEYNHIDAIIGLPANIFFGTGIPTIIM
VLKQKRENTDVLIVDASKGFAKVGKNNVLRACDIKKIVDVVSARADVEKFAKVVSLDEIRGNDYNLNIPRYVDSSDKNER
WDIFATMFGGIPLSELADFHEYWAAFPHLKDALFTASGEAHCRLNVANLKSSVLDAADVTAFTARYASSFADFEAFLTTR
LLDGMETLNITREKSIISDDVFSRLNGLPLIDKYKAYQLLDDEWKKIAVDLEIIRTEGFASSKQVDPNMVVKTKDGKKQE
VQDGWIGHIIPFALVQQTLLAADLARLNAREARLSELAAEYESLLNELSEEDKDKPFVNDEGDAFVPKEVAKAIKSMEVE
PETLAVLRKVNALNNEEKSLKKQIKADSTALHLLTKKTIEELTDEQARQMIKLKWIPPVLDSMAGLPSAVIADFIARLLA
LAGKYETTFTEVDSQIAETESSLVSLLDELTGNEFDMQGIQELKSLLGGSAHE
>Mature_853_residues
MNKQQLASKIWESANKMRSKIEANEYKDYILGFIFYKFLSDKEVKFLKENDWTDDDLPHVTEDDAETVDYIRSNVGYFIS
YKHLFSTWIDKGSDFNAADVTEALSAFSRLINPSHKKVFDKVFATLETGLSKLGENSGARTKAIRDLLHLIKDIPMDGRQ
DYDVLGFIYEYLISNFAANAGKKAGEFYTPHEVSLLMSEIVAAHLKDRQQIKIYDPTSGSGSLLINIGKCVARYMGGGDN
IKYYAQELKENTYNLTRMNLVMRGILPNNIVTRNGDTLEEDWPYFDDNDPVNTYDPLYVDAVVSNPPYSQSWNPADKESD
PRYRFGLAPKGKADYAFLLHDLYHLKPDGIMTIVLPHGVLFRGGTEGAIRRNLVEYNHIDAIIGLPANIFFGTGIPTIIM
VLKQKRENTDVLIVDASKGFAKVGKNNVLRACDIKKIVDVVSARADVEKFAKVVSLDEIRGNDYNLNIPRYVDSSDKNER
WDIFATMFGGIPLSELADFHEYWAAFPHLKDALFTASGEAHCRLNVANLKSSVLDAADVTAFTARYASSFADFEAFLTTR
LLDGMETLNITREKSIISDDVFSRLNGLPLIDKYKAYQLLDDEWKKIAVDLEIIRTEGFASSKQVDPNMVVKTKDGKKQE
VQDGWIGHIIPFALVQQTLLAADLARLNAREARLSELAAEYESLLNELSEEDKDKPFVNDEGDAFVPKEVAKAIKSMEVE
PETLAVLRKVNALNNEEKSLKKQIKADSTALHLLTKKTIEELTDEQARQMIKLKWIPPVLDSMAGLPSAVIADFIARLLA
LAGKYETTFTEVDSQIAETESSLVSLLDELTGNEFDMQGIQELKSLLGGSAHE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=286, Percent_Identity=25.1748251748252, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 95769; Mature: 95769

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQQLASKIWESANKMRSKIEANEYKDYILGFIFYKFLSDKEVKFLKENDWTDDDLPHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
TEDDAETVDYIRSNVGYFISYKHLFSTWIDKGSDFNAADVTEALSAFSRLINPSHKKVFD
CCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
KVFATLETGLSKLGENSGARTKAIRDLLHLIKDIPMDGRQDYDVLGFIYEYLISNFAANA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKKAGEFYTPHEVSLLMSEIVAAHLKDRQQIKIYDPTSGSGSLLINIGKCVARYMGGGDN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHCCCCC
IKYYAQELKENTYNLTRMNLVMRGILPNNIVTRNGDTLEEDWPYFDDNDPVNTYDPLYVD
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
AVVSNPPYSQSWNPADKESDPRYRFGLAPKGKADYAFLLHDLYHLKPDGIMTIVLPHGVL
EEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEE
FRGGTEGAIRRNLVEYNHIDAIIGLPANIFFGTGIPTIIMVLKQKRENTDVLIVDASKGF
EECCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCCCCEEEEECCCCH
AKVGKNNVLRACDIKKIVDVVSARADVEKFAKVVSLDEIRGNDYNLNIPRYVDSSDKNER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
WDIFATMFGGIPLSELADFHEYWAAFPHLKDALFTASGEAHCRLNVANLKSSVLDAADVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
AFTARYASSFADFEAFLTTRLLDGMETLNITREKSIISDDVFSRLNGLPLIDKYKAYQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DDEWKKIAVDLEIIRTEGFASSKQVDPNMVVKTKDGKKQEVQDGWIGHIIPFALVQQTLL
HHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHH
AADLARLNAREARLSELAAEYESLLNELSEEDKDKPFVNDEGDAFVPKEVAKAIKSMEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PETLAVLRKVNALNNEEKSLKKQIKADSTALHLLTKKTIEELTDEQARQMIKLKWIPPVL
HHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DSMAGLPSAVIADFIARLLALAGKYETTFTEVDSQIAETESSLVSLLDELTGNEFDMQGI
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QELKSLLGGSAHE
HHHHHHHCCCCCC
>Mature Secondary Structure
MNKQQLASKIWESANKMRSKIEANEYKDYILGFIFYKFLSDKEVKFLKENDWTDDDLPHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCC
TEDDAETVDYIRSNVGYFISYKHLFSTWIDKGSDFNAADVTEALSAFSRLINPSHKKVFD
CCCHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
KVFATLETGLSKLGENSGARTKAIRDLLHLIKDIPMDGRQDYDVLGFIYEYLISNFAANA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKKAGEFYTPHEVSLLMSEIVAAHLKDRQQIKIYDPTSGSGSLLINIGKCVARYMGGGDN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHCCCCC
IKYYAQELKENTYNLTRMNLVMRGILPNNIVTRNGDTLEEDWPYFDDNDPVNTYDPLYVD
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
AVVSNPPYSQSWNPADKESDPRYRFGLAPKGKADYAFLLHDLYHLKPDGIMTIVLPHGVL
EEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCEE
FRGGTEGAIRRNLVEYNHIDAIIGLPANIFFGTGIPTIIMVLKQKRENTDVLIVDASKGF
EECCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCHHHHHHHHHHHCCCCCEEEEECCCCH
AKVGKNNVLRACDIKKIVDVVSARADVEKFAKVVSLDEIRGNDYNLNIPRYVDSSDKNER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
WDIFATMFGGIPLSELADFHEYWAAFPHLKDALFTASGEAHCRLNVANLKSSVLDAADVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
AFTARYASSFADFEAFLTTRLLDGMETLNITREKSIISDDVFSRLNGLPLIDKYKAYQLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DDEWKKIAVDLEIIRTEGFASSKQVDPNMVVKTKDGKKQEVQDGWIGHIIPFALVQQTLL
HHHHHHHHHHHEEEECCCCCCCCCCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHH
AADLARLNAREARLSELAAEYESLLNELSEEDKDKPFVNDEGDAFVPKEVAKAIKSMEVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PETLAVLRKVNALNNEEKSLKKQIKADSTALHLLTKKTIEELTDEQARQMIKLKWIPPVL
HHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
DSMAGLPSAVIADFIARLLALAGKYETTFTEVDSQIAETESSLVSLLDELTGNEFDMQGI
HHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QELKSLLGGSAHE
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]