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The map label for this gene is nlaIIIM [H]

Identifier: 187736388

GI number: 187736388

Start: 2310879

End: 2311973

Strand: Direct

Name: nlaIIIM [H]

Synonym: Amuc_1905

Alternate gene names: 187736388

Gene position: 2310879-2311973 (Clockwise)

Preceding gene: 187736386

Following gene: 187736389

Centisome position: 86.74

GC content: 53.24

Gene sequence:

>1095_bases
ATGGAGGAAGATCCCAGATTCCTGACGGAACAAATTGTAACCTATTTGGGCAATAAGCGTTCCCTTCTTCATTTTCTGGG
GACGGGGCTGGAACAGGTGAAAAAACGCCTGGGTAAGGAAAGGCTGAAAGCAGGGGATCTTTTTTCCGGCAGCGGCATTG
TGGCGCGTTTTCTGAAACAGCATTCGGAAGACTTGATCGTCAATGATCTGGAGGAATACAGCCGGGTCGTCAATACCTGT
TACCTGAGCAACGCGGAGGAAGTGGAAAATCTGGAGCTGGACAGACATTACAGACGCCTGCTGCGGTATATGGAAACGCA
TGAATCCCCTGGCTTCATCACGGAACTGTACGCTCCCAGAAATCCGGACTGCATCACGGCGGATGACCGCGTTTTCTATA
CGCGCCGCAACGCCGTGTACCTGGATACGGCGAGGCAGACCATCAATTTGCTTCCGGCAGAAGTCAGGCCCTATTTTATC
GCTCCTCTTCTGGCGGAAGCTTCCGTCCACACAAACACATCCGGCGTATTCAAAGGTTTTTACAAAGACAGGAACGGAAT
CGGCAAATTCGGGGGAACGGGAGGCAACGCCCTTTCCCGCATTCTGGGAGAAATTTCGCTGCCGTTTCCCGTGTTTTCCC
GGTTTGACTGCCGGTATTCCATCCATTGCCGTGACGCCAATGAACTGGCTGCCGAGCTGCCGGAAATGGATGTGGTGTAC
CTGGACCCTCCCTATAACCAGCATCCGTACGGTTCCAATTATTTCATGCTGAATTTGCTGTCTTCTTATGAAAAGCCGGA
GGAAACCAGCCGAGTTTCCGGCATTCCCGCAGATTGGAAACGTTCCACGTACAATAGCCGCCAGCATGCTCCGGAGGCCC
TGTTCCGGCTGCTGGAGGATTGCCCGGCCAAATTTATTCTGCTTTCCTACAGCTCGGAGGGGTTCATCAACTATGAGGAG
ATGACCGGTTTCCTTGGACGCCTGGGCCATACCGTTACGCTGGAAACGCCGTACGCCGCGTTCCGGGGCAGCCGGAATTT
CAGAAACCGTCCTCAGAACGTGACGGAATTCCTGTTCCTGGTGGAACGGTTTTAA

Upstream 100 bases:

>100_bases
CGCACCCCGGAGCAACTTGACGGAAAGTCCGGAGTGCCGTATGCTGGACGGGACCTGCTATATGACCCGGAACGAGCCAT
CATCCCTTCCAGCTCCGCAG

Downstream 100 bases:

>100_bases
CAGATGACAGGAAAAGCAGCCTCCATGCAAACTCATTTGGAACGCTTCATCCGTTTTCTGGCCGCGGAGAAAGGCCTGAG
CACCGCCTACCAGCTTTCCG

Product: Site-specific DNA-methyltransferase (adenine-specific)

Products: NA

Alternate protein names: M.NlaIII; Adenine-specific methyltransferase NlaIII [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEEDPRFLTEQIVTYLGNKRSLLHFLGTGLEQVKKRLGKERLKAGDLFSGSGIVARFLKQHSEDLIVNDLEEYSRVVNTC
YLSNAEEVENLELDRHYRRLLRYMETHESPGFITELYAPRNPDCITADDRVFYTRRNAVYLDTARQTINLLPAEVRPYFI
APLLAEASVHTNTSGVFKGFYKDRNGIGKFGGTGGNALSRILGEISLPFPVFSRFDCRYSIHCRDANELAAELPEMDVVY
LDPPYNQHPYGSNYFMLNLLSSYEKPEETSRVSGIPADWKRSTYNSRQHAPEALFRLLEDCPAKFILLSYSSEGFINYEE
MTGFLGRLGHTVTLETPYAAFRGSRNFRNRPQNVTEFLFLVERF

Sequences:

>Translated_364_residues
MEEDPRFLTEQIVTYLGNKRSLLHFLGTGLEQVKKRLGKERLKAGDLFSGSGIVARFLKQHSEDLIVNDLEEYSRVVNTC
YLSNAEEVENLELDRHYRRLLRYMETHESPGFITELYAPRNPDCITADDRVFYTRRNAVYLDTARQTINLLPAEVRPYFI
APLLAEASVHTNTSGVFKGFYKDRNGIGKFGGTGGNALSRILGEISLPFPVFSRFDCRYSIHCRDANELAAELPEMDVVY
LDPPYNQHPYGSNYFMLNLLSSYEKPEETSRVSGIPADWKRSTYNSRQHAPEALFRLLEDCPAKFILLSYSSEGFINYEE
MTGFLGRLGHTVTLETPYAAFRGSRNFRNRPQNVTEFLFLVERF
>Mature_364_residues
MEEDPRFLTEQIVTYLGNKRSLLHFLGTGLEQVKKRLGKERLKAGDLFSGSGIVARFLKQHSEDLIVNDLEEYSRVVNTC
YLSNAEEVENLELDRHYRRLLRYMETHESPGFITELYAPRNPDCITADDRVFYTRRNAVYLDTARQTINLLPAEVRPYFI
APLLAEASVHTNTSGVFKGFYKDRNGIGKFGGTGGNALSRILGEISLPFPVFSRFDCRYSIHCRDANELAAELPEMDVVY
LDPPYNQHPYGSNYFMLNLLSSYEKPEETSRVSGIPADWKRSTYNSRQHAPEALFRLLEDCPAKFILLSYSSEGFINYEE
MTGFLGRLGHTVTLETPYAAFRGSRNFRNRPQNVTEFLFLVERF

Specific function: This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41831; Mature: 41831

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDPRFLTEQIVTYLGNKRSLLHFLGTGLEQVKKRLGKERLKAGDLFSGSGIVARFLKQ
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
HSEDLIVNDLEEYSRVVNTCYLSNAEEVENLELDRHYRRLLRYMETHESPGFITELYAPR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
NPDCITADDRVFYTRRNAVYLDTARQTINLLPAEVRPYFIAPLLAEASVHTNTSGVFKGF
CCCEECCCCEEEEEECCEEEEECHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCHHHHHHH
YKDRNGIGKFGGTGGNALSRILGEISLPFPVFSRFDCRYSIHCRDANELAAELPEMDVVY
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCHHHHHHHCCCCCEEE
LDPPYNQHPYGSNYFMLNLLSSYEKPEETSRVSGIPADWKRSTYNSRQHAPEALFRLLED
ECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHH
CPAKFILLSYSSEGFINYEEMTGFLGRLGHTVTLETPYAAFRGSRNFRNRPQNVTEFLFL
CCCEEEEEEECCCCCEEHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHH
VERF
HHCC
>Mature Secondary Structure
MEEDPRFLTEQIVTYLGNKRSLLHFLGTGLEQVKKRLGKERLKAGDLFSGSGIVARFLKQ
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
HSEDLIVNDLEEYSRVVNTCYLSNAEEVENLELDRHYRRLLRYMETHESPGFITELYAPR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
NPDCITADDRVFYTRRNAVYLDTARQTINLLPAEVRPYFIAPLLAEASVHTNTSGVFKGF
CCCEECCCCEEEEEECCEEEEECHHHHHHHCCHHCCHHHHHHHHHHHHCCCCCHHHHHHH
YKDRNGIGKFGGTGGNALSRILGEISLPFPVFSRFDCRYSIHCRDANELAAELPEMDVVY
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCHHHHHHHCCCCCEEE
LDPPYNQHPYGSNYFMLNLLSSYEKPEETSRVSGIPADWKRSTYNSRQHAPEALFRLLED
ECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHH
CPAKFILLSYSSEGFINYEEMTGFLGRLGHTVTLETPYAAFRGSRNFRNRPQNVTEFLFL
CCCEEEEEEECCCCCEEHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCCCCHHHHHHHHH
VERF
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]