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The map label for this gene is cysJ [H]

Identifier: 187736382

GI number: 187736382

Start: 2306040

End: 2306474

Strand: Reverse

Name: cysJ [H]

Synonym: Amuc_1899

Alternate gene names: 187736382

Gene position: 2306474-2306040 (Counterclockwise)

Preceding gene: 187736383

Following gene: 187736377

Centisome position: 86.58

GC content: 51.95

Gene sequence:

>435_bases
ATGGAAATCACTATTATCTACGGAACGGAAACTGGCAATTCGGAGAGTCTGGCCCAGGACGCACGGGCAAAATTGACCAA
GCTCGGCCATCAGGCAAAGGTGGTTGACATGGAAGGTATAACGGTGGAACAGTTGAAGAATGCGGGAACGCTGCTGGTCA
TCACCAGTACGTGGGGGGACGGAGAACCTCCCACCAACGCAGAGGCCCTCCATCAGGAATTGAAGGACTCTTCGGAAGAT
TTGTCCGAGGTAAGCTACGCCGTTTTTGCCCTTGGAGATGAATTTTACGAAAACTTCTGCCAGGCTGGCAAGGACTTCGA
TTCCTTCCTGGAACGTTTGAAAGCGCAAAGGCTTCTGCCGGTAGTGCTTTCCAATGGAGACCATGATGATACCTTCCCGG
AATGGGTGGATGCCATCGCGGAAAAACTCTCCTGA

Upstream 100 bases:

>100_bases
ATAAACAAGGAAGAAAAATTCAGCCGTCGTCCTGCTGTTTTGCCTTGCCTGTTTAATTAGTTTAATCTAACTTTCGTTGT
CAAACCAATCATCAGAACGC

Downstream 100 bases:

>100_bases
TGTTTTTTCAAGGTTTGCAATACCTTATCCTCAAAAGAGCGCTTCTTCCGGGATGAACGTCTGGAGGAAGCGCTTTTTCC
TGAAAAAAGCAGGAACTCTC

Product: flavodoxin/nitric oxide synthase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGITVEQLKNAGTLLVITSTWGDGEPPTNAEALHQELKDSSED
LSEVSYAVFALGDEFYENFCQAGKDFDSFLERLKAQRLLPVVLSNGDHDDTFPEWVDAIAEKLS

Sequences:

>Translated_144_residues
MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGITVEQLKNAGTLLVITSTWGDGEPPTNAEALHQELKDSSED
LSEVSYAVFALGDEFYENFCQAGKDFDSFLERLKAQRLLPVVLSNGDHDDTFPEWVDAIAEKLS
>Mature_144_residues
MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGITVEQLKNAGTLLVITSTWGDGEPPTNAEALHQELKDSSED
LSEVSYAVFALGDEFYENFCQAGKDFDSFLERLKAQRLLPVVLSNGDHDDTFPEWVDAIAEKLS

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=139, Percent_Identity=38.1294964028777, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI127139033, Length=141, Percent_Identity=31.2056737588652, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789123, Length=145, Percent_Identity=39.3103448275862, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789154, Length=117, Percent_Identity=41.025641025641, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1790181, Length=139, Percent_Identity=30.2158273381295, Blast_Score=60, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17554134, Length=150, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17566446, Length=158, Percent_Identity=31.6455696202532, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322597, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137192, Length=144, Percent_Identity=30.5555555555556, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 15838; Mature: 15838

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGITVEQLKNAGTLLVITSTWGD
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEEEEEECCCC
GEPPTNAEALHQELKDSSEDLSEVSYAVFALGDEFYENFCQAGKDFDSFLERLKAQRLLP
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVLSNGDHDDTFPEWVDAIAEKLS
HHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGITVEQLKNAGTLLVITSTWGD
CEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCEEEEEECCCC
GEPPTNAEALHQELKDSSEDLSEVSYAVFALGDEFYENFCQAGKDFDSFLERLKAQRLLP
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VVLSNGDHDDTFPEWVDAIAEKLS
HHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]