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The map label for this gene is gltX

Identifier: 187736371

GI number: 187736371

Start: 2293947

End: 2295248

Strand: Reverse

Name: gltX

Synonym: Amuc_1888

Alternate gene names: 187736371

Gene position: 2295248-2293947 (Counterclockwise)

Preceding gene: 187736374

Following gene: 187736370

Centisome position: 86.15

GC content: 58.29

Gene sequence:

>1302_bases
ATGAGTCTCGTCAGAACAAGATTTGCACCTTCCCCCACGGGCTACCTTCACATCGGGGGCGCCCGCACCGCCCTGTTCAA
CTGGCTGTTTGCCCGCAAGATGGGGGGAACCTTCATCCTCCGCATTGAAGATACGGATAACGCCCGCAACACGGAGGAGG
CTACCCGCGCAATCTTCACCGGCATGGAATGGCTGGGGCTGGACTGGGATGAGGGGCCCATGAAGGGCGGCGATTGCGGT
CCCTATTTCCAAAGCCAGCGCAACGATATTTACGACGCCTATTTCAAGAAGCTCCAGGACGCCGGCCGCGTGTACGAGGA
TGAAGGAGCCTGGCGTTTCCGCTTTGACCGCTCCAAACCCGTCACCTTCCATGACCTGATTTGCGGAAACATCACCATCG
ACTACCGGGACGCCTCCAACACCCCGGACATGGCTATCCGCCGCGCGGACGGTTCCTACATCTTCCACTTTGTCAACGTG
GTGGACGATATTGAGATGAAGATGACCCATGTCATTCGCGGGGAAGACCACATCATGAACACGCCCAAGCACATCCAGCT
GTTTGAGGCTTTCGGCGTCACTCCCCCTGTTTTCGCCCACATGCCGCTGATTTTGAACCAGGACGGCTCCAAAATGTCCA
AGCGCGACGTGGGCGCTGCCCTGGGAACCTACCCGGAAGAAGGCTTTCTGCCGGAAGGCGTCATGAACTTTCTGGCCCTG
CTGGGCTGGTCTCCCAAGGACGATACGGAAATTTTCTCCCCTCAGGAGCTGATCGGACGCTTCTCTCTGGAAGCCGTGAA
CCACTCCGCCGCCAAATTCGATATCACCAAATGCCGCTGGGTCAACCAGCAGCACATCATCGCCCTGCCTGCGGAAGAAT
TCGCCCTCCGGGCACGCCCCTTCTGTTTGAAAGCCGGCCTGCCGGATTCCCCGATTCTGGACGCCGCCATCGCCACCGTG
CAAACGAAGGTGCAGACTCTGGCGGAAGTCCCGGATAAAATCCGTTTCTTTTTTGACCTGGGCATGGATCCGGAAGCCGT
CTCCAAAGTTCAGCCGGAAGCCCTGGAACTGCTTTTCAAACTGGCGGACAGGCTGGAACAGGAACCGGAATGGGATGGAC
ATGAACTCATTGGCGTGCTGAAAGCCTTTGCCAAGGAAAACGGCGTAAAAATGGGAGCCGTGATGTTCCCGGCCCGGGTG
GCCCTCACCGGGTTAAGCGGCGGCCCGGACCTTTCCTCCGTCTTTTCCCTGCTGGGCCGGGAGGAAACCGTAAGGAGAAT
CCGTTCCTTTTCTCTGGACTGA

Upstream 100 bases:

>100_bases
ACGTCCTAAAAAATCCACTGCTTCCGCATCCCCGTTTTTTGGCATGCACCCCCTGTATCTGCTTGACGCGGGCAGACAGG
CCCTTACTCTCTTACCCATC

Downstream 100 bases:

>100_bases
TTCCCGCCGGAGTATTCATCTTTTCAAACGCACCGGAAATTCTCCGGTGCGGTTCCTGTTTACGGAAGGGCATCTTCCGG
GGATTCGGGAAATGATGCCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSLVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTDNARNTEEATRAIFTGMEWLGLDWDEGPMKGGDCG
PYFQSQRNDIYDAYFKKLQDAGRVYEDEGAWRFRFDRSKPVTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNV
VDDIEMKMTHVIRGEDHIMNTPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAALGTYPEEGFLPEGVMNFLAL
LGWSPKDDTEIFSPQELIGRFSLEAVNHSAAKFDITKCRWVNQQHIIALPAEEFALRARPFCLKAGLPDSPILDAAIATV
QTKVQTLAEVPDKIRFFFDLGMDPEAVSKVQPEALELLFKLADRLEQEPEWDGHELIGVLKAFAKENGVKMGAVMFPARV
ALTGLSGGPDLSSVFSLLGREETVRRIRSFSLD

Sequences:

>Translated_433_residues
MSLVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTDNARNTEEATRAIFTGMEWLGLDWDEGPMKGGDCG
PYFQSQRNDIYDAYFKKLQDAGRVYEDEGAWRFRFDRSKPVTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNV
VDDIEMKMTHVIRGEDHIMNTPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAALGTYPEEGFLPEGVMNFLAL
LGWSPKDDTEIFSPQELIGRFSLEAVNHSAAKFDITKCRWVNQQHIIALPAEEFALRARPFCLKAGLPDSPILDAAIATV
QTKVQTLAEVPDKIRFFFDLGMDPEAVSKVQPEALELLFKLADRLEQEPEWDGHELIGVLKAFAKENGVKMGAVMFPARV
ALTGLSGGPDLSSVFSLLGREETVRRIRSFSLD
>Mature_432_residues
SLVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTDNARNTEEATRAIFTGMEWLGLDWDEGPMKGGDCGP
YFQSQRNDIYDAYFKKLQDAGRVYEDEGAWRFRFDRSKPVTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNVV
DDIEMKMTHVIRGEDHIMNTPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAALGTYPEEGFLPEGVMNFLALL
GWSPKDDTEIFSPQELIGRFSLEAVNHSAAKFDITKCRWVNQQHIIALPAEEFALRARPFCLKAGLPDSPILDAAIATVQ
TKVQTLAEVPDKIRFFFDLGMDPEAVSKVQPEALELLFKLADRLEQEPEWDGHELIGVLKAFAKENGVKMGAVMFPARVA
LTGLSGGPDLSSVFSLLGREETVRRIRSFSLD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=30.7535641547862, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=37.2591006423983, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=35.1239669421488, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=476, Percent_Identity=31.0924369747899, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=30.9859154929577, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321192, Length=117, Percent_Identity=32.4786324786325, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=31.4814814814815, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_AKKM8 (B2UN91)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878483.1
- GeneID:   6274856
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1888
- HOGENOM:   HBG628189
- OMA:   IVRIATT
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48607; Mature: 48476

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTDNARNTEEATRAIFT
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
GMEWLGLDWDEGPMKGGDCGPYFQSQRNDIYDAYFKKLQDAGRVYEDEGAWRFRFDRSKP
CHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCEEECCCCEEEEECCCCC
VTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNVVDDIEMKMTHVIRGEDHIMN
CCHHHEEECCEEEEECCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCHHHCC
TPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAALGTYPEEGFLPEGVMNFLAL
CCHHHHHHHHHCCCCHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LGWSPKDDTEIFSPQELIGRFSLEAVNHSAAKFDITKCRWVNQQHIIALPAEEFALRARP
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECHHHEECCCCCEEEECHHHHHHHCCC
FCLKAGLPDSPILDAAIATVQTKVQTLAEVPDKIRFFFDLGMDPEAVSKVQPEALELLFK
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCHHHHHHCCHHHHHHHHH
LADRLEQEPEWDGHELIGVLKAFAKENGVKMGAVMFPARVALTGLSGGPDLSSVFSLLGR
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEHEECCHHHEEECCCCCCCHHHHHHHHCC
EETVRRIRSFSLD
HHHHHHHHHCCCC
>Mature Secondary Structure 
SLVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTDNARNTEEATRAIFT
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
GMEWLGLDWDEGPMKGGDCGPYFQSQRNDIYDAYFKKLQDAGRVYEDEGAWRFRFDRSKP
CHHHHCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCEEECCCCEEEEECCCCC
VTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNVVDDIEMKMTHVIRGEDHIMN
CCHHHEEECCEEEEECCCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCHHHCC
TPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAALGTYPEEGFLPEGVMNFLAL
CCHHHHHHHHHCCCCHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LGWSPKDDTEIFSPQELIGRFSLEAVNHSAAKFDITKCRWVNQQHIIALPAEEFALRARP
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEECHHHEECCCCCEEEECHHHHHHHCCC
FCLKAGLPDSPILDAAIATVQTKVQTLAEVPDKIRFFFDLGMDPEAVSKVQPEALELLFK
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCHHHHHHCCHHHHHHHHH
LADRLEQEPEWDGHELIGVLKAFAKENGVKMGAVMFPARVALTGLSGGPDLSSVFSLLGR
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEHEECCHHHEEECCCCCCCHHHHHHHHCC
EETVRRIRSFSLD
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA