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The map label for this gene is nikA [H]

Identifier: 187736336

GI number: 187736336

Start: 2252547

End: 2254751

Strand: Reverse

Name: nikA [H]

Synonym: Amuc_1853

Alternate gene names: 187736336

Gene position: 2254751-2252547 (Counterclockwise)

Preceding gene: 187736337

Following gene: 187736335

Centisome position: 84.63

GC content: 53.97

Gene sequence:

>2205_bases
ATGCTGTCCCTTCTGTTGACAGCCGGGTCCGGTTGCAAGGATTCCTCCCAATCCCAGGGGGTAGGCTGGCAGCCGAATGT
ACCCTTCGGCACACTCCCACCGGGATTTGATTCCTTCCCGGAACGATGGAACAAACAGATCAATGACCGCCTGGCTCGTG
AGGAAGCTGCCAAGCAAAAGGAAATACGGGAGCTCCGGGACAAGTTTTTTAAAGAGGAAGATTCTAAAGTCAGGGAAAAA
CTGCAAAGCAAGCTCATTGCGGATGAAGCCGCCCTGTCCGTCATTCACCGCAGGCAGACGGAAGGCGACTACATCAAATT
CAAAACCCCGGCGGACATCCCCCAAGACCTGAAGTGGGAGGATGGACTGGACAATCCGGAGATAGGGGATCCGAACGCCA
AAAAAGGAGGCGTGCTGCGCCAGTGGGCCCCCGGCTCCTATCCCGACACATTCCGGCCCAACGGCCCGAACAGCAACAGC
GGTTTCCGCGGTCCCCTGTATGATGAAATCATCATCGGCCTCGTTTCCATCCATCCGGTTACGGGAAAAATCATTCCAGG
AATCGCTCATAAATGGGCGGAATCCCCAGACAGGCGCACTGTTTACTTCGAACTGGATCCGGATGCCCGCTATACGGACG
GAGCCAAGGTAAAGGCCATCGACCTCCTGGTGAACATGTATATCCGCACTTCGGAATACAGCCGGGACGTATTCTACAAC
AACTTTTTTTACCAGAACGCTTCCAACATCACCATCTACGACGACAGCCGCTTTTCCATCACACTCCCCTTCGCCAAACC
TCTGCTCCCGTACTATTGCACGCTGTTCATTCCGTCCCCGCCGCATTTTTATTGCGAGTTCGGCCCCAACTACGTGGAAC
GCTACCAGTGGCGCATACCGCCCACTACGGGCGCTTACGTTGTCAAGCCGGACGGTATCATCCGCGGCCGCCAGGTAACG
CTCCAGCGCGTGCCGGACTGGTGGGCCAAGGACAAAAAATTCACGAAATACATGTATAATGTGGATCAAATCGTGTATAA
TTTCATTGCGGAACCATCCAAGGCCATCGAACTCTTCCGCATTGGAGAACTGGACGTTTTGAACATCACCAAACCGGAAC
TATGGCACGAACGCATGGAAATACCGGAGGTTCACAACGGCTACATCAACCGCAGCACATTTTACACCATTTACCCCCGC
CCTCCTTACGGAGTTTTTCTGAATACCTCCAAAGCCCCCTTCAATGACCTCAATGTTCGCCGGGGGTTCCAGCACGCACT
TAATATCCAGAATATCATTGACATTACCTTCCGCGGAGACTACCAGCGGCTCAATTCCTACAATTCAGGTTTCGGCAAAT
TCACCAATCCTTATATCAAGGCGCGCCCCTATTCCCCGGAACAGGCCCGAGCTTATTTCGCCAGGGCGGGCTACACCATC
CCCTGCCCGGACGGTATCCTCCGCAAACCGGACGGCACCCGGCTCACCGCAGCCATCACCTTCCCCAATTCATCTCCTTC
CCTGGCTTCCACTCTGGGCAAGCTGAAGGAAGACGCGCGCAAGTGCGGCCTGGAAATCCAGCTTGACCCTCTGGACTCCA
CCGTAGCTTTCCGCAAAATCATGGAAAAACGCGCTCAGGCCTCCTTCATGGCCTGGGGATTCACTCCCCCCCATCCGATG
AACGAGCAGGGGTTCCACTCCCGGTACGCCTATGATGAGCGTGGAGGCCTTATTACCTATACCAATAACATCTGCGCTTA
CGCGGACAAAGAAATGGACAAGCTGCTGGACGCAGAAACAAACGCAGCCACGGAAGACGAACTCCAAAAGGCCACGTGGA
AAGTGCAGCAGAAAATTGATGACGAAGCCCTGTGGGTTCCCTGCTGGACCACGGAATTCATCAGACTGGGTTACTGGCGC
TGGGTCAAATGGCCGAACAGTGCAACTACGCAATTCTGCCATCCCGTCGTATTTGATCCCATGGAAAGTTATCTGTACTG
GGTGGATAACGATATCAAAAAGGAAACAATGGAAGCCAAGCGGGAAGGAAAAACATTCGAGGAAGTGGATACCGTGTATG
ACCAGTATCGCTACATGGACTCCATTGAAAGCCTGGATAACAAGGATGGCGGCGGCAAGCTGCCGTCTGTGCCTGTCATC
CCGGAAAGCGGCGACCCTCTGGAACCTTCTGCCACGGAAAAATAA

Upstream 100 bases:

>100_bases
GACCCTAAAAAATTCACCACTTATAAATAAAAAGACTCCCGCTTTGCCATTTCTTATGCTTAAGCTGTCCACCCTCCTCC
GTTCCACCCTTTCTTCCGTG

Downstream 100 bases:

>100_bases
CACATGAGCGATCCCCTGTTGGAAATCAAGAACCTGGTCACCGGCTTCGAAACGGAATCCGGCCTGTTGAAAGCGGTGGA
CGGCGTCAGCTTCACCGTCC

Product: extracellular solute-binding protein family 5

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MLSLLLTAGSGCKDSSQSQGVGWQPNVPFGTLPPGFDSFPERWNKQINDRLAREEAAKQKEIRELRDKFFKEEDSKVREK
LQSKLIADEAALSVIHRRQTEGDYIKFKTPADIPQDLKWEDGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFRPNGPNSNS
GFRGPLYDEIIIGLVSIHPVTGKIIPGIAHKWAESPDRRTVYFELDPDARYTDGAKVKAIDLLVNMYIRTSEYSRDVFYN
NFFYQNASNITIYDDSRFSITLPFAKPLLPYYCTLFIPSPPHFYCEFGPNYVERYQWRIPPTTGAYVVKPDGIIRGRQVT
LQRVPDWWAKDKKFTKYMYNVDQIVYNFIAEPSKAIELFRIGELDVLNITKPELWHERMEIPEVHNGYINRSTFYTIYPR
PPYGVFLNTSKAPFNDLNVRRGFQHALNIQNIIDITFRGDYQRLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTI
PCPDGILRKPDGTRLTAAITFPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKIMEKRAQASFMAWGFTPPHPM
NEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAETNAATEDELQKATWKVQQKIDDEALWVPCWTTEFIRLGYWR
WVKWPNSATTQFCHPVVFDPMESYLYWVDNDIKKETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVI
PESGDPLEPSATEK

Sequences:

>Translated_734_residues
MLSLLLTAGSGCKDSSQSQGVGWQPNVPFGTLPPGFDSFPERWNKQINDRLAREEAAKQKEIRELRDKFFKEEDSKVREK
LQSKLIADEAALSVIHRRQTEGDYIKFKTPADIPQDLKWEDGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFRPNGPNSNS
GFRGPLYDEIIIGLVSIHPVTGKIIPGIAHKWAESPDRRTVYFELDPDARYTDGAKVKAIDLLVNMYIRTSEYSRDVFYN
NFFYQNASNITIYDDSRFSITLPFAKPLLPYYCTLFIPSPPHFYCEFGPNYVERYQWRIPPTTGAYVVKPDGIIRGRQVT
LQRVPDWWAKDKKFTKYMYNVDQIVYNFIAEPSKAIELFRIGELDVLNITKPELWHERMEIPEVHNGYINRSTFYTIYPR
PPYGVFLNTSKAPFNDLNVRRGFQHALNIQNIIDITFRGDYQRLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTI
PCPDGILRKPDGTRLTAAITFPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKIMEKRAQASFMAWGFTPPHPM
NEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAETNAATEDELQKATWKVQQKIDDEALWVPCWTTEFIRLGYWR
WVKWPNSATTQFCHPVVFDPMESYLYWVDNDIKKETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVI
PESGDPLEPSATEK
>Mature_734_residues
MLSLLLTAGSGCKDSSQSQGVGWQPNVPFGTLPPGFDSFPERWNKQINDRLAREEAAKQKEIRELRDKFFKEEDSKVREK
LQSKLIADEAALSVIHRRQTEGDYIKFKTPADIPQDLKWEDGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFRPNGPNSNS
GFRGPLYDEIIIGLVSIHPVTGKIIPGIAHKWAESPDRRTVYFELDPDARYTDGAKVKAIDLLVNMYIRTSEYSRDVFYN
NFFYQNASNITIYDDSRFSITLPFAKPLLPYYCTLFIPSPPHFYCEFGPNYVERYQWRIPPTTGAYVVKPDGIIRGRQVT
LQRVPDWWAKDKKFTKYMYNVDQIVYNFIAEPSKAIELFRIGELDVLNITKPELWHERMEIPEVHNGYINRSTFYTIYPR
PPYGVFLNTSKAPFNDLNVRRGFQHALNIQNIIDITFRGDYQRLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTI
PCPDGILRKPDGTRLTAAITFPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKIMEKRAQASFMAWGFTPPHPM
NEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAETNAATEDELQKATWKVQQKIDDEALWVPCWTTEFIRLGYWR
WVKWPNSATTQFCHPVVFDPMESYLYWVDNDIKKETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVI
PESGDPLEPSATEK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=456, Percent_Identity=22.1491228070175, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 84342; Mature: 84342

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLLLTAGSGCKDSSQSQGVGWQPNVPFGTLPPGFDSFPERWNKQINDRLAREEAAKQK
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIRELRDKFFKEEDSKVREKLQSKLIADEAALSVIHRRQTEGDYIKFKTPADIPQDLKWE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFRPNGPNSNSGFRGPLYDEIIIGLVSIHPV
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TGKIIPGIAHKWAESPDRRTVYFELDPDARYTDGAKVKAIDLLVNMYIRTSEYSRDVFYN
CCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCEEC
NFFYQNASNITIYDDSRFSITLPFAKPLLPYYCTLFIPSPPHFYCEFGPNYVERYQWRIP
CEEEECCCEEEEEECCEEEEECCCHHHHHCEEEEEEECCCCCEEEECCCCHHHHEEEECC
PTTGAYVVKPDGIIRGRQVTLQRVPDWWAKDKKFTKYMYNVDQIVYNFIAEPSKAIELFR
CCCCEEEECCCCEECCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEE
IGELDVLNITKPELWHERMEIPEVHNGYINRSTFYTIYPRPPYGVFLNTSKAPFNDLNVR
CCCEEEEECCCHHHHHHHCCCCCHHCCEECCCEEEEEECCCCCCEEEECCCCCCCCCHHH
RGFQHALNIQNIIDITFRGDYQRLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTI
HHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCC
PCPDGILRKPDGTRLTAAITFPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKI
CCCCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
MEKRAQASFMAWGFTPPHPMNEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAET
HHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECHHHHHHHHCCCC
NAATEDELQKATWKVQQKIDDEALWVPCWTTEFIRLGYWRWVKWPNSATTQFCHPVVFDP
CCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCEEEEECCCCCCHHHCCCHHHCC
MESYLYWVDNDIKKETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVI
HHHHEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PESGDPLEPSATEK
CCCCCCCCCCCCCC
>Mature Secondary Structure
MLSLLLTAGSGCKDSSQSQGVGWQPNVPFGTLPPGFDSFPERWNKQINDRLAREEAAKQK
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EIRELRDKFFKEEDSKVREKLQSKLIADEAALSVIHRRQTEGDYIKFKTPADIPQDLKWE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
DGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFRPNGPNSNSGFRGPLYDEIIIGLVSIHPV
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TGKIIPGIAHKWAESPDRRTVYFELDPDARYTDGAKVKAIDLLVNMYIRTSEYSRDVFYN
CCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCEEC
NFFYQNASNITIYDDSRFSITLPFAKPLLPYYCTLFIPSPPHFYCEFGPNYVERYQWRIP
CEEEECCCEEEEEECCEEEEECCCHHHHHCEEEEEEECCCCCEEEECCCCHHHHEEEECC
PTTGAYVVKPDGIIRGRQVTLQRVPDWWAKDKKFTKYMYNVDQIVYNFIAEPSKAIELFR
CCCCEEEECCCCEECCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHEEEEE
IGELDVLNITKPELWHERMEIPEVHNGYINRSTFYTIYPRPPYGVFLNTSKAPFNDLNVR
CCCEEEEECCCHHHHHHHCCCCCHHCCEECCCEEEEEECCCCCCEEEECCCCCCCCCHHH
RGFQHALNIQNIIDITFRGDYQRLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTI
HHHHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCC
PCPDGILRKPDGTRLTAAITFPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKI
CCCCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
MEKRAQASFMAWGFTPPHPMNEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAET
HHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECHHHHHHHHCCCC
NAATEDELQKATWKVQQKIDDEALWVPCWTTEFIRLGYWRWVKWPNSATTQFCHPVVFDP
CCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCEEEEECCCCCCHHHCCCHHHCC
MESYLYWVDNDIKKETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVI
HHHHEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PESGDPLEPSATEK
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]