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The map label for this gene is ygfA [C]

Identifier: 187736248

GI number: 187736248

Start: 2144037

End: 2144627

Strand: Direct

Name: ygfA [C]

Synonym: Amuc_1761

Alternate gene names: 187736248

Gene position: 2144037-2144627 (Clockwise)

Preceding gene: 187736247

Following gene: 187736254

Centisome position: 80.48

GC content: 62.77

Gene sequence:

>591_bases
ATGGAAGGAAAAGCATCCCAATCCGGAACCGTAAGCGGTCCGGCGGCTGCCAAACGGGAAGTGCGCCGCATGATACGTGA
AAGGGTGGGCGGATTGGATGCCGCCGCCCGGGAAGCCATGTCCCGTTCCATCTGCCTCCGTGTGTCGGCTCTGGTGCGGG
AGAAGAATTTCCGGCGCGTGGCTGTTTTTGCCGCCAGGCCGGGAGAGGTGGATTTGCTTTCCCTGCTGGATCTGCTTCCG
GACCGGGAATGGTACTTCCCGCTGGTGCATGAGGGGAGAAGGCTGAGTTTTCACCGTGTATGCCATCACGGGGAATTGGT
GGCGGGCAGCTGGGGCATACGCGAACCCGGTCCGGGATGCCCGGAACTGCATGCGGGGGAAATGGATTTGATCGTGCTGC
CTGGGGCCGCATTTACCAGGGACGGCAAGCGGCTGGGGTATGGCGGCGGATTTTATGATACGCTGCTGGCCGGCCCGGCT
GCTGGGGTGCCGCTGGCGGGAGTCTGCTTCCCCTGCCAGCTTCTGGACGATCTGCCCATGGAAGCCCATGACAGGCATGT
GGACATGGTGATTGCGCCGGGAGGGGAGTAA

Upstream 100 bases:

>100_bases
GGCTGAAGGAGATGGTGAAGGAAGACGGCCGTGCCGTTTGGCTGGAGGATGGGCATCCGCGCAACTCCCTGGAAAGCGTA
TTTCTAAAAAGTTTGTTGGA

Downstream 100 bases:

>100_bases
CAGTAACACCCTTCCGGAGAAAGGGAAAGAGGCTAGATACGGAACCGGTGTTTCTGCACGGTCAGTTTATAAAACAGGTC
GTAGCGGGCGTTGAGCAGGG

Product: 5-formyltetrahydrofolate cyclo-ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MEGKASQSGTVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSICLRVSALVREKNFRRVAVFAARPGEVDLLSLLDLLP
DREWYFPLVHEGRRLSFHRVCHHGELVAGSWGIREPGPGCPELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPA
AGVPLAGVCFPCQLLDDLPMEAHDRHVDMVIAPGGE

Sequences:

>Translated_196_residues
MEGKASQSGTVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSICLRVSALVREKNFRRVAVFAARPGEVDLLSLLDLLP
DREWYFPLVHEGRRLSFHRVCHHGELVAGSWGIREPGPGCPELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPA
AGVPLAGVCFPCQLLDDLPMEAHDRHVDMVIAPGGE
>Mature_196_residues
MEGKASQSGTVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSICLRVSALVREKNFRRVAVFAARPGEVDLLSLLDLLP
DREWYFPLVHEGRRLSFHRVCHHGELVAGSWGIREPGPGCPELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPA
AGVPLAGVCFPCQLLDDLPMEAHDRHVDMVIAPGGE

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=177, Percent_Identity=30.5084745762712, Blast_Score=67, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321031, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161077430, Length=191, Percent_Identity=27.2251308900524, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 21178; Mature: 21178

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKASQSGTVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSICLRVSALVREKNFRRV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVFAARPGEVDLLSLLDLLPDREWYFPLVHEGRRLSFHRVCHHGELVAGSWGIREPGPGC
EEEECCCCCHHHHHHHHHCCCCCEECHHHHCCCCCHHHHHHCCCCEEECCCCCCCCCCCC
PELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPAAGVPLAGVCFPCQLLDDLPM
CCCCCCCEEEEEECCCCCCCCCCEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
EAHDRHVDMVIAPGGE
CCCCCEEEEEEECCCC
>Mature Secondary Structure
MEGKASQSGTVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSICLRVSALVREKNFRRV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVFAARPGEVDLLSLLDLLPDREWYFPLVHEGRRLSFHRVCHHGELVAGSWGIREPGPGC
EEEECCCCCHHHHHHHHHCCCCCEECHHHHCCCCCHHHHHHCCCCEEECCCCCCCCCCCC
PELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPAAGVPLAGVCFPCQLLDDLPM
CCCCCCCEEEEEECCCCCCCCCCEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
EAHDRHVDMVIAPGGE
CCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]