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The map label for this gene is btuB [H]

Identifier: 187736171

GI number: 187736171

Start: 2043845

End: 2045851

Strand: Direct

Name: btuB [H]

Synonym: Amuc_1684

Alternate gene names: 187736171

Gene position: 2043845-2045851 (Clockwise)

Preceding gene: 187736164

Following gene: 187736172

Centisome position: 76.72

GC content: 55.11

Gene sequence:

>2007_bases
ATGCTTTATACGAAGAAAGCCCTTCAAATGGGCGCAATTGCTGTTGGCCTTGCCGCATTCGCAGGCCAGTCCTCTCTTGC
CGAGTCCGCTAAAAAGGAAGACTCCAAGCCCTCCGTCAGGACGGAAACCATGCAGGTTATGCCGGAACTGACTATGGCGT
CCCACTTTGTGGGCGTGCCGTACAACCGGTCTGGAGTGTCCGTTTCCATCATCAATCCGGAAGAATTCCAGAAGGCGGGC
ATTGAAACCCTGACGGGAGCCCTTTCCCAGACGCCCGGCGTTTTCACGCTGGACGGAGGCGGCACCTGGCAGCGCGGTTC
CGTGAGCAACACCGTCATCCGCGGGATGAACAAGGATACCTACACCCTGACCATGGTTGACGGCATGCGCATCAGTGATG
CCAACATGTCCGGCAACAAGCTGCTGGGCATCACCAATCTCTTTACGGTGGGCAATGTGGAAGTGGTGAAAGGAGCGCAG
GGCGCCGTTTTCGGTTCCGGCGCCATTGGAGGCGTTGTCGCGATGGATACTCCGGAGGGGGAAGGCGATCCCGTAACCAG
GATTTTTGCGGAGGCCGGTTCCTTCGGCTCTTTCAACAGCTATGTCACTTCCTCCGGCAAGATCAAGAAGCTTTCCTATT
TTGTAGGCGTTGGGTTTGAAACTACGGAAAACGATCCGTCAATCTATCCGGCCATTTATGACAACAGGACAGGCATGAAC
GATTTCCGCCAGTGGCAGGAAGCCGTGCGCCTGGGCTATGACATCAATGACAAGGTGAAGGTGAGCTTTACCTATCGCCG
CCTGGATTCCTACTTTGAATATCCCACGCCGTATGTGGATTATAACCAGTGGCCTTCCGTTCCGGAGCCCCATCTGTACA
ATACGGAAGACAAGAACCGCAGCAACTTGGTGACAGGACGTGTGGATGCGGAAATTTCCAAGCTGTGGTCCACCAGCTTC
ATGGTGGGGCATTACAACATGGACTATTCCTGCCATACTCCCGGATTTGACTTCCAGCCCAACGTGATGCGCAACCGCCG
CTTCCAGGCGGAGTGGCGCAATGCCCTGACGTGGAACAAGGAATGGAAAACCATCGCCGGCATGGCCTGGGACCGTTCCG
ACTACATGAGCGAAAACAATTACGTTGCCAAGGATGAATGGCAGAGCACGCTTGCCTTCTTTGCAGAGCAAATGTGGTCA
CCCACGGACAGCTTTGACGCCAGCGTGGCCCTGCGCCTGGAACATGATTCCGTCTGGAACAATCATTTTACGTGGCGTTA
TTCCAATTCCTGGAAAGTGACGGGCAAGGATTCCCCCACCCGTATTTTCGGTTCCGTAGGGTCCGGTTTCCGCGCGCCCA
CCTGGTTTGAGCAGTATGCGGCCAATTACGGTTATGTAGGCAACCCTGATCTGGATGTGTCCAAGTCCCTGGGCGGCGAC
CTGGGTGTGGAACAGCGCCTGGCGGACGGCCATTATGCTTCCGTGACGGGCTTCTGGACCCGCATTAACGATGAAATCGG
CACCAAGAGCGTAGGTACCTGGCCGAACTCCTATACCACTTACGCCAATTATTCCCACTGCACTTCCTACGGTGTGGAAG
TGGCGTTCAAGGGTCAGTTCAAGGACGCGTGGAACAGCGGCTATTATGCCAATTACACCTTCACGATGCCCAAGCGCGAT
TCCATCGGCAAATACGAGACCATTCAGATGGCCAATACCGCCCGCCACACCGTCAACGCAGAGGTTTATACCTCCCCGGT
TGAAAAGCTTACGGTTGGCTTCGGCGTAACGGCCGCCATGGGACGCACGGACTACAACTATGCCCGTCTGGATAATTTCT
TTACGGCGCGCCTGTTTGCCCGTTACCAGGCAACGGACAATGTGTCCCTCCATGTGCGTCTGGAAAACCTGTTTGACCAG
AAGTTCATCATGACGAATGATTATAACTTCGGTCCGCGTGAAGCCCGTGGATTCGGAATCTTCGGCGGTGTGACGGTCGA
ATTCTAA

Upstream 100 bases:

>100_bases
GGTACCGCCACTGTAAGCGAGCCTGCCTCGCAAGCCAGAAAACCAGCCTGTGAGGTTGTCCAACTGTTCGGCGAGTTACC
GAACGTAAGGCACTACAACC

Downstream 100 bases:

>100_bases
GTTCCTTTCAATATTAATTGTTGATTGCTCCCCCATCTTCCGCCTTGCGGACTGTTTCAGGCGAAGTGAACGGTTCCGCG
CGGAAGATGGGTTTTCTGTT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MLYTKKALQMGAIAVGLAAFAGQSSLAESAKKEDSKPSVRTETMQVMPELTMASHFVGVPYNRSGVSVSIINPEEFQKAG
IETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDTYTLTMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQ
GAVFGSGAIGGVVAMDTPEGEGDPVTRIFAEAGSFGSFNSYVTSSGKIKKLSYFVGVGFETTENDPSIYPAIYDNRTGMN
DFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPEPHLYNTEDKNRSNLVTGRVDAEISKLWSTSF
MVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNALTWNKEWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWS
PTDSFDASVALRLEHDSVWNNHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTWFEQYAANYGYVGNPDLDVSKSLGGD
LGVEQRLADGHYASVTGFWTRINDEIGTKSVGTWPNSYTTYANYSHCTSYGVEVAFKGQFKDAWNSGYYANYTFTMPKRD
SIGKYETIQMANTARHTVNAEVYTSPVEKLTVGFGVTAAMGRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQ
KFIMTNDYNFGPREARGFGIFGGVTVEF

Sequences:

>Translated_668_residues
MLYTKKALQMGAIAVGLAAFAGQSSLAESAKKEDSKPSVRTETMQVMPELTMASHFVGVPYNRSGVSVSIINPEEFQKAG
IETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDTYTLTMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQ
GAVFGSGAIGGVVAMDTPEGEGDPVTRIFAEAGSFGSFNSYVTSSGKIKKLSYFVGVGFETTENDPSIYPAIYDNRTGMN
DFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPEPHLYNTEDKNRSNLVTGRVDAEISKLWSTSF
MVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNALTWNKEWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWS
PTDSFDASVALRLEHDSVWNNHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTWFEQYAANYGYVGNPDLDVSKSLGGD
LGVEQRLADGHYASVTGFWTRINDEIGTKSVGTWPNSYTTYANYSHCTSYGVEVAFKGQFKDAWNSGYYANYTFTMPKRD
SIGKYETIQMANTARHTVNAEVYTSPVEKLTVGFGVTAAMGRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQ
KFIMTNDYNFGPREARGFGIFGGVTVEF
>Mature_668_residues
MLYTKKALQMGAIAVGLAAFAGQSSLAESAKKEDSKPSVRTETMQVMPELTMASHFVGVPYNRSGVSVSIINPEEFQKAG
IETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDTYTLTMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQ
GAVFGSGAIGGVVAMDTPEGEGDPVTRIFAEAGSFGSFNSYVTSSGKIKKLSYFVGVGFETTENDPSIYPAIYDNRTGMN
DFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPEPHLYNTEDKNRSNLVTGRVDAEISKLWSTSF
MVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNALTWNKEWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWS
PTDSFDASVALRLEHDSVWNNHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTWFEQYAANYGYVGNPDLDVSKSLGGD
LGVEQRLADGHYASVTGFWTRINDEIGTKSVGTWPNSYTTYANYSHCTSYGVEVAFKGQFKDAWNSGYYANYTFTMPKRD
SIGKYETIQMANTARHTVNAEVYTSPVEKLTVGFGVTAAMGRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQ
KFIMTNDYNFGPREARGFGIFGGVTVEF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=432, Percent_Identity=24.0740740740741, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 74741; Mature: 74741

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTKKALQMGAIAVGLAAFAGQSSLAESAKKEDSKPSVRTETMQVMPELTMASHFVGVP
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCC
YNRSGVSVSIINPEEFQKAGIETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDT
CCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCCCCCE
YTLTMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQGAVFGSGAIGGVVAMDTPEG
EEEEEECCEEEECCCCCCCEEEEEEEEEEECCEEEEECCCCCEEECCCCCCEEEEECCCC
EGDPVTRIFAEAGSFGSFNSYVTSSGKIKKLSYFVGVGFETTENDPSIYPAIYDNRTGMN
CCCHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEEECCEEECCCCCCEEEEEECCCCCHH
DFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPEPHLYNTEDKNR
HHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SNLVTGRVDAEISKLWSTSFMVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNALTWNK
CCEEEEECCHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCC
EWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWSPTDSFDASVALRLEHDSVWN
CCCEEECEEECCHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTWFEQYAANYGYVGNPDLDVSKSLGGD
CCEEEEECCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCC
LGVEQRLADGHYASVTGFWTRINDEIGTKSVGTWPNSYTTYANYSHCTSYGVEVAFKGQF
CCHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHCCCEEEEEECCC
KDAWNSGYYANYTFTMPKRDSIGKYETIQMANTARHTVNAEVYTSPVEKLTVGFGVTAAM
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCHHHEECCEEEECCHHHEEECCCCEECC
GRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQKFIMTNDYNFGPREARGFGI
CCCCCCHHHHHHHHHEEHHHEEECCCCCEEEEEEHHHCCCEEEEECCCCCCCCCCCCEEE
FGGVTVEF
ECCEEEEC
>Mature Secondary Structure
MLYTKKALQMGAIAVGLAAFAGQSSLAESAKKEDSKPSVRTETMQVMPELTMASHFVGVP
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHEECCC
YNRSGVSVSIINPEEFQKAGIETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDT
CCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCCCCCE
YTLTMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQGAVFGSGAIGGVVAMDTPEG
EEEEEECCEEEECCCCCCCEEEEEEEEEEECCEEEEECCCCCEEECCCCCCEEEEECCCC
EGDPVTRIFAEAGSFGSFNSYVTSSGKIKKLSYFVGVGFETTENDPSIYPAIYDNRTGMN
CCCHHHHHHHHCCCCCCCCCEEECCCCEEEEEEEEECCEEECCCCCCEEEEEECCCCCHH
DFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPEPHLYNTEDKNR
HHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SNLVTGRVDAEISKLWSTSFMVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNALTWNK
CCEEEEECCHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCC
EWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWSPTDSFDASVALRLEHDSVWN
CCCEEECEEECCHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTWFEQYAANYGYVGNPDLDVSKSLGGD
CCEEEEECCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHCCCC
LGVEQRLADGHYASVTGFWTRINDEIGTKSVGTWPNSYTTYANYSHCTSYGVEVAFKGQF
CCHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHCCCEEEEEECCC
KDAWNSGYYANYTFTMPKRDSIGKYETIQMANTARHTVNAEVYTSPVEKLTVGFGVTAAM
CCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCHHHEECCEEEECCHHHEEECCCCEECC
GRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQKFIMTNDYNFGPREARGFGI
CCCCCCHHHHHHHHHEEHHHEEECCCCCEEEEEEHHHCCCEEEEECCCCCCCCCCCCEEE
FGGVTVEF
ECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA