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The map label for this gene is cobS [H]

Identifier: 187736167

GI number: 187736167

Start: 2037957

End: 2038697

Strand: Reverse

Name: cobS [H]

Synonym: Amuc_1680

Alternate gene names: 187736167

Gene position: 2038697-2037957 (Counterclockwise)

Preceding gene: 187736168

Following gene: 187736166

Centisome position: 76.52

GC content: 65.99

Gene sequence:

>741_bases
ATGGCTGTCATCACCACCATCCGCTCCGCCTTCGGCTTCCTGACCCGGCTGCCCGTAGGGCCGTGGCCGCTGCAAAATGA
CCTGAACGGAATTTCCGCCTGGCTACCGCTGGTTGGCCTGGTAGTGGGGGGGCTTGCGGGCGGCCTGACCTGGACGACAA
CCCTGCTCTTCCCCCCCCTGGTCTGCGGCGTTATCGGCTGTGCCTGCTGGGTAGCCGTCACGGGCGGCCTGCACCTGGAC
GGAGTAGCGGACTGCGGAGACGGGCTTCCCATTGAAGTATCCCGGGAACGCAGGCTGGAAATCATGAAAGACTCCCGCCT
GGGGACCTTCGGCGGCACGGCCCTGTTTTTCAACCTGGCGTTCAAGGGAGCCGCCCTGGCGACTCTGGCGGCCCACGGCT
CCTGGGAACTTCTGCTCACGGCGTGCGCTCTGGCAGGATTGCTTGCCCGCAGCCAGATATTCATCGCCATGCGCTTTCCG
GGCGCACGGCATGGAGGCATGGGGGAAGCGTTCAAGCAGGGAACGCGCCCCGGGCACGCCCTGGCTGCCGCAGCCGTCAC
CCTGGCTGCCTGCGCTCTGGCAGGATGGAACGGGCTGTACGCCCTGCTCTCCGCCCTGGCCGGCTCCATGGGCCTGCTTC
TATACGCGCGCCGCCGCCTGGGCGGCGTTACCGGGGATGTATTCGGCTGTACCGTGGAATGTACGGAATGGCTTGTCCTG
CTCACCTTCTGCACTTTATGA

Upstream 100 bases:

>100_bases
TTTCCCCTGCTGGACGCCGCCACGCGCGTACTTTCTGAAATGAAAACCCTGCCGGAACTGGACATCACCGTTCCGCGCTG
ACCCTTTCTTCCCTGTATCC

Downstream 100 bases:

>100_bases
AAACTCCACACAAATGCCGCGTTCCAGGCCTCAACATCGGATGCACCTCCTTCATCATCCCGGACTACTACGTGCCCGCC
ATCAGGGAATGCGTCCATTA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGGLAGGLTWTTTLLFPPLVCGVIGCACWVAVTGGLHLD
GVADCGDGLPIEVSRERRLEIMKDSRLGTFGGTALFFNLAFKGAALATLAAHGSWELLLTACALAGLLARSQIFIAMRFP
GARHGGMGEAFKQGTRPGHALAAAAVTLAACALAGWNGLYALLSALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVL
LTFCTL

Sequences:

>Translated_246_residues
MAVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGGLAGGLTWTTTLLFPPLVCGVIGCACWVAVTGGLHLD
GVADCGDGLPIEVSRERRLEIMKDSRLGTFGGTALFFNLAFKGAALATLAAHGSWELLLTACALAGLLARSQIFIAMRFP
GARHGGMGEAFKQGTRPGHALAAAAVTLAACALAGWNGLYALLSALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVL
LTFCTL
>Mature_245_residues
AVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGGLAGGLTWTTTLLFPPLVCGVIGCACWVAVTGGLHLDG
VADCGDGLPIEVSRERRLEIMKDSRLGTFGGTALFFNLAFKGAALATLAAHGSWELLLTACALAGLLARSQIFIAMRFPG
ARHGGMGEAFKQGTRPGHALAAAAVTLAACALAGWNGLYALLSALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVLL
TFCTL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=236, Percent_Identity=35.5932203389831, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25546; Mature: 25415

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGGLAGGLTWTTTLLFPPL
CHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCGVIGCACWVAVTGGLHLDGVADCGDGLPIEVSRERRLEIMKDSRLGTFGGTALFFNLA
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHH
FKGAALATLAAHGSWELLLTACALAGLLARSQIFIAMRFPGARHGGMGEAFKQGTRPGHA
HCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHCCCCCHH
LAAAAVTLAACALAGWNGLYALLSALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHH
LTFCTL
HHHHCC
>Mature Secondary Structure 
AVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGGLAGGLTWTTTLLFPPL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VCGVIGCACWVAVTGGLHLDGVADCGDGLPIEVSRERRLEIMKDSRLGTFGGTALFFNLA
HHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHH
FKGAALATLAAHGSWELLLTACALAGLLARSQIFIAMRFPGARHGGMGEAFKQGTRPGHA
HCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHCCCCCHH
LAAAAVTLAACALAGWNGLYALLSALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHH
LTFCTL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA