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The map label for this gene is lacZ [H]

Identifier: 187736154

GI number: 187736154

Start: 2017753

End: 2020329

Strand: Direct

Name: lacZ [H]

Synonym: Amuc_1667

Alternate gene names: 187736154

Gene position: 2017753-2020329 (Clockwise)

Preceding gene: 187736153

Following gene: 187736156

Centisome position: 75.74

GC content: 59.95

Gene sequence:

>2577_bases
ATGCGCAAGATGATGGCCTGCATGCTGGGCGCTGCCCTGTGTTTTTCCATGGCCGGAGCGGAAACAGGCGTTTCCTCTGC
GGAAAAAGCTTCACCCCTGTTCGGGAGGATGATCTGGAATCGCGGCTGGGAGTTCCGGCTTGAGGACGAGCCCGGAAAGA
GCGGCTGGCAGACGGTTCATCTGCCCCATACGTTCAGTCTGCCCTACTTTATGTCCGATTCCTTTTACACGGGGTTCGGC
TCCTACCGCAAGAAGCTTGTCATGCCGGAGGAATGGCAGGGGAAGAAGGTGTTTCTGGATTGCGGCGCAGCTTTCCAGGT
GGCGGAGGTGAAGGTGAATGGGAAGACTGCGGGAAGGCATGAAGGGGGGTATACGGCCTTCCGGTCGGATTTGACGCCTT
TCCTGAAGCCCGGCGAGAACTGGGTGGAGGTACGGGTGGATAACCGCTGGAGCCCCCGCATTGCGCCTCGGGCGGGGGAG
CATACTTTTTCCGGAGGACTGTACCGGAACGTGCATCTGCACGTGTGTTCTCCCGTGTATTTGCCCGCGCACGGAGTATG
GGTTCAGACGCCGGAGGTGACGGCGGCCCGCGGGAAGGTCCGGATTTTGACGGAAGTGAAGAATGATACCGGGACAGTGA
GGAAAGTGACGGTGCGGCATACTGTGCGGGATGAGCAAAGCGGCTGCGTGGTGCTGCGGGGAGAGAAGGGCGCGGAACTG
GGGGCCGGGGAAGATTCTCGCGTACAGGCTGACCTGCCTCCTCTGGCTTCTCCCAGGTTGTGGAGCCCTGCGGCCCCCAA
TATGTACCGTGTGACGACGGAATTGTTGGACGAGAAGGGGAAGGTGCTGGACCGGGCTGAAAATCCCCTGGGTTTCCGTA
CTCTGGAGCTGACGGCGGACCAGGGTATGCTGATTAACGGGAAACCTGTTTATCTGCAGGGGGCCAATGTGCACCAGGAC
CACGCCGGGTGGGGGGATGCCGTGACGGATGCCGGGGCCCGCCGTGATGTACGGCTGATGAAGAACGCCGGGTTTAATTT
CATCCGCGGCTCCCATTACCCTCATTCCCAGGCTTTTCTGGATGCTTGCGACCGGGAGGGCATGTGCATGCTGAATGAAG
GCATTTTCTGGGGAATGGGCGGGTTCAAGGAGCATGACAGATACTGGAATTGCGATGCCTATCCTGCGGAAAAGAAGGAT
CGTATTGCGTTTGAGGAAAGCTGCATGCGCCAGGTGAGGGAGATGGTGCTTCAATTCCGCAATCATCCTTCCATCGTCAT
CTGGAGCATCAGCAATGAACCGTTTTTTACCAGGCATGCGCCGGAGGCCAGGGCTTTGTGCAACAGGCTCATTACCTTGG
TGAAGGAGTTGGACCCGACGCGCCCCGTGTGCGCCGGGGGCGGGCAGCGCGGAAATTTTGACAAGCTGGGGGACATGGCC
GCCTTGAACGGGGACGGTTCCCATGTGAAGACGCCCGGCAGGCCCAGCATGGTGACGGAGTATGGTTCCGTGAGCTGCCG
GAGGCCGGGGGCGTATGCGCCGGGCTGGGGGGATATGAAGAAGGATAAGGAAGCGGGGATTCGCTATCCCTGGCGCGTGG
GAGAGGCGGTCTGGTGCGGCTTTGACCACGGCAGCATCTGGCCTTCCGGAGGGCGCATGGGCATTGTGGATTACTTCCGC
ATTCCCAAGCGGGCCTGGTACTGGTACAGGAATGCGTTGAGGAATATTCCTCCTCCGGAGTGGCCTGTAGAGGGGACTCC
GGCACAGGTGAAACTGTCCGCGGACAAGAAGGTTATCAGCCCGGCGGACGGCACGGACGATGTGCATGTGACCGTGAAAG
TGGCGGACGCCGCCGGAAGGCAGATATCCAATGCCGTGCCGGTGACGCTTACGGTGGTATCGGGTCCCGGGGAGTTCCCT
ACCGGCAAAAGCATTACGTTCACGCCCGGAACGGATATTGATTTAATTGACGGCTGTGCGGCGATTGAGTTTCGGTCTTA
TTATGCCGGGAAGACAGTGATCAGGGCGTCTTCTCCCGGATTGAAGGGGGATAGCCTCCAAATCGTTTGCCGGAATGCTC
CGGCTTACGTAGCGGGCAGGAGCGCGGAAACGCGGGAAAGGCCCTACAAGCGTTTTAGCGCAAAGGAGAGGGATATCCAG
CTGGCGCGGTACGGACGGCCCGAATCCGGAGAGAAAGCCAATTTGGCTGTATTGAGGCCCTGTTCCGCTTCCTCCGGATT
TCAGGAGACGATGAAGGCTTCCGACGGTGACGATGTTTCTGCATGGCATCCGTCCGTGGAAGACAAAGCGCCGTGGTGGC
AGCTGGACATGGAGTTTGAATTCCGCCTGGACCGGGTGGAGGTGAAGGCAGCCGGAAGCTGGAAGGGGCCGGCTCCCTCG
GTTCAGGTCAGCAGGGACGGAAATAGCTGGAAGGACATTAAAGCCGTTTTGCGCAAGGATGGGACGGAGCTGACCGCAAT
GTGTCCGGAAGGGACCGGCGCCAGGTACGTACGCATCCGCCTGTCTCCGGGGCAGGGAATTGCGGAAGTGGCCGTATGGC
CGGCGGATGCGTCATGA

Upstream 100 bases:

>100_bases
GCGCCCTTTCCGGGGTGCACGGCTTGTTGTTGCGGGCTTTTCAGGGTGCGGAAATGTTTTTTTAATGAAAGAAAGAACAT
GGCGGAATGGTAACATACGC

Downstream 100 bases:

>100_bases
GGGATCCCGTTTTTCCGGAACAGGAGGCTTTTTTCTGTTACTGCGCGTACCGGAGTTTCCGGCGTCCTCTTCCGCCCGCT
GGCGGCGCAGTTTGTCCCAA

Product: glycoside hydrolase family 2 sugar binding

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 858; Mature: 858

Protein sequence:

>858_residues
MRKMMACMLGAALCFSMAGAETGVSSAEKASPLFGRMIWNRGWEFRLEDEPGKSGWQTVHLPHTFSLPYFMSDSFYTGFG
SYRKKLVMPEEWQGKKVFLDCGAAFQVAEVKVNGKTAGRHEGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGE
HTFSGGLYRNVHLHVCSPVYLPAHGVWVQTPEVTAARGKVRILTEVKNDTGTVRKVTVRHTVRDEQSGCVVLRGEKGAEL
GAGEDSRVQADLPPLASPRLWSPAAPNMYRVTTELLDEKGKVLDRAENPLGFRTLELTADQGMLINGKPVYLQGANVHQD
HAGWGDAVTDAGARRDVRLMKNAGFNFIRGSHYPHSQAFLDACDREGMCMLNEGIFWGMGGFKEHDRYWNCDAYPAEKKD
RIAFEESCMRQVREMVLQFRNHPSIVIWSISNEPFFTRHAPEARALCNRLITLVKELDPTRPVCAGGGQRGNFDKLGDMA
ALNGDGSHVKTPGRPSMVTEYGSVSCRRPGAYAPGWGDMKKDKEAGIRYPWRVGEAVWCGFDHGSIWPSGGRMGIVDYFR
IPKRAWYWYRNALRNIPPPEWPVEGTPAQVKLSADKKVISPADGTDDVHVTVKVADAAGRQISNAVPVTLTVVSGPGEFP
TGKSITFTPGTDIDLIDGCAAIEFRSYYAGKTVIRASSPGLKGDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQ
LARYGRPESGEKANLAVLRPCSASSGFQETMKASDGDDVSAWHPSVEDKAPWWQLDMEFEFRLDRVEVKAAGSWKGPAPS
VQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIRLSPGQGIAEVAVWPADAS

Sequences:

>Translated_858_residues
MRKMMACMLGAALCFSMAGAETGVSSAEKASPLFGRMIWNRGWEFRLEDEPGKSGWQTVHLPHTFSLPYFMSDSFYTGFG
SYRKKLVMPEEWQGKKVFLDCGAAFQVAEVKVNGKTAGRHEGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGE
HTFSGGLYRNVHLHVCSPVYLPAHGVWVQTPEVTAARGKVRILTEVKNDTGTVRKVTVRHTVRDEQSGCVVLRGEKGAEL
GAGEDSRVQADLPPLASPRLWSPAAPNMYRVTTELLDEKGKVLDRAENPLGFRTLELTADQGMLINGKPVYLQGANVHQD
HAGWGDAVTDAGARRDVRLMKNAGFNFIRGSHYPHSQAFLDACDREGMCMLNEGIFWGMGGFKEHDRYWNCDAYPAEKKD
RIAFEESCMRQVREMVLQFRNHPSIVIWSISNEPFFTRHAPEARALCNRLITLVKELDPTRPVCAGGGQRGNFDKLGDMA
ALNGDGSHVKTPGRPSMVTEYGSVSCRRPGAYAPGWGDMKKDKEAGIRYPWRVGEAVWCGFDHGSIWPSGGRMGIVDYFR
IPKRAWYWYRNALRNIPPPEWPVEGTPAQVKLSADKKVISPADGTDDVHVTVKVADAAGRQISNAVPVTLTVVSGPGEFP
TGKSITFTPGTDIDLIDGCAAIEFRSYYAGKTVIRASSPGLKGDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQ
LARYGRPESGEKANLAVLRPCSASSGFQETMKASDGDDVSAWHPSVEDKAPWWQLDMEFEFRLDRVEVKAAGSWKGPAPS
VQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIRLSPGQGIAEVAVWPADAS
>Mature_858_residues
MRKMMACMLGAALCFSMAGAETGVSSAEKASPLFGRMIWNRGWEFRLEDEPGKSGWQTVHLPHTFSLPYFMSDSFYTGFG
SYRKKLVMPEEWQGKKVFLDCGAAFQVAEVKVNGKTAGRHEGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGE
HTFSGGLYRNVHLHVCSPVYLPAHGVWVQTPEVTAARGKVRILTEVKNDTGTVRKVTVRHTVRDEQSGCVVLRGEKGAEL
GAGEDSRVQADLPPLASPRLWSPAAPNMYRVTTELLDEKGKVLDRAENPLGFRTLELTADQGMLINGKPVYLQGANVHQD
HAGWGDAVTDAGARRDVRLMKNAGFNFIRGSHYPHSQAFLDACDREGMCMLNEGIFWGMGGFKEHDRYWNCDAYPAEKKD
RIAFEESCMRQVREMVLQFRNHPSIVIWSISNEPFFTRHAPEARALCNRLITLVKELDPTRPVCAGGGQRGNFDKLGDMA
ALNGDGSHVKTPGRPSMVTEYGSVSCRRPGAYAPGWGDMKKDKEAGIRYPWRVGEAVWCGFDHGSIWPSGGRMGIVDYFR
IPKRAWYWYRNALRNIPPPEWPVEGTPAQVKLSADKKVISPADGTDDVHVTVKVADAAGRQISNAVPVTLTVVSGPGEFP
TGKSITFTPGTDIDLIDGCAAIEFRSYYAGKTVIRASSPGLKGDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQ
LARYGRPESGEKANLAVLRPCSASSGFQETMKASDGDDVSAWHPSVEDKAPWWQLDMEFEFRLDRVEVKAAGSWKGPAPS
VQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIRLSPGQGIAEVAVWPADAS

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=445, Percent_Identity=24.9438202247191, Blast_Score=130, Evalue=4e-30,
Organism=Escherichia coli, GI1787903, Length=583, Percent_Identity=24.5283018867925, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI48994920, Length=510, Percent_Identity=24.5098039215686, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI1786539, Length=493, Percent_Identity=26.1663286004057, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=473, Percent_Identity=23.6786469344609, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI62471735, Length=454, Percent_Identity=25.5506607929515, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI19922582, Length=454, Percent_Identity=25.5506607929515, Blast_Score=132, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24655438, Length=454, Percent_Identity=25.5506607929515, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24651745, Length=498, Percent_Identity=24.2971887550201, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 94831; Mature: 94831

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50022 FA58C_3 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKMMACMLGAALCFSMAGAETGVSSAEKASPLFGRMIWNRGWEFRLEDEPGKSGWQTVH
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCCCCCEEEE
LPHTFSLPYFMSDSFYTGFGSYRKKLVMPEEWQGKKVFLDCGAAFQVAEVKVNGKTAGRH
CCEEECCCEEECCCCCCCCHHHHHEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCC
EGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGEHTFSGGLYRNVHLHVCSPVY
CCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCEE
LPAHGVWVQTPEVTAARGKVRILTEVKNDTGTVRKVTVRHTVRDEQSGCVVLRGEKGAEL
ECCCCEEEECCCCEECCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCC
GAGEDSRVQADLPPLASPRLWSPAAPNMYRVTTELLDEKGKVLDRAENPLGFRTLELTAD
CCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHCCCCCCEEEEEEECC
QGMLINGKPVYLQGANVHQDHAGWGDAVTDAGARRDVRLMKNAGFNFIRGSHYPHSQAFL
CCEEECCCEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHCCCCEECCCCCCCHHHHH
DACDREGMCMLNEGIFWGMGGFKEHDRYWNCDAYPAEKKDRIAFEESCMRQVREMVLQFR
HHCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NHPSIVIWSISNEPFFTRHAPEARALCNRLITLVKELDPTRPVCAGGGQRGNFDKLGDMA
CCCCEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHCCEE
ALNGDGSHVKTPGRPSMVTEYGSVSCRRPGAYAPGWGDMKKDKEAGIRYPWRVGEAVWCG
EECCCCCCCCCCCCCCEEECCCCCEECCCCCCCCCCCCCCCHHHCCCCCCEECCCEEEEE
FDHGSIWPSGGRMGIVDYFRIPKRAWYWYRNALRNIPPPEWPVEGTPAQVKLSADKKVIS
CCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEC
PADGTDDVHVTVKVADAAGRQISNAVPVTLTVVSGPGEFPTGKSITFTPGTDIDLIDGCA
CCCCCCCEEEEEEEECCCCCHHCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHCCEE
AIEFRSYYAGKTVIRASSPGLKGDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQ
EEEEHHHHCCCEEEEECCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCEE
LARYGRPESGEKANLAVLRPCSASSGFQETMKASDGDDVSAWHPSVEDKAPWWQLDMEFE
HEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
FRLDRVEVKAAGSWKGPAPSVQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIR
EEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEE
LSPGQGIAEVAVWPADAS
ECCCCCEEEEEEECCCCC
>Mature Secondary Structure
MRKMMACMLGAALCFSMAGAETGVSSAEKASPLFGRMIWNRGWEFRLEDEPGKSGWQTVH
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCCCCCEEEE
LPHTFSLPYFMSDSFYTGFGSYRKKLVMPEEWQGKKVFLDCGAAFQVAEVKVNGKTAGRH
CCEEECCCEEECCCCCCCCHHHHHEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCC
EGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGEHTFSGGLYRNVHLHVCSPVY
CCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCEE
LPAHGVWVQTPEVTAARGKVRILTEVKNDTGTVRKVTVRHTVRDEQSGCVVLRGEKGAEL
ECCCCEEEECCCCEECCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCC
GAGEDSRVQADLPPLASPRLWSPAAPNMYRVTTELLDEKGKVLDRAENPLGFRTLELTAD
CCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHHCCCCCCEEEEEEECC
QGMLINGKPVYLQGANVHQDHAGWGDAVTDAGARRDVRLMKNAGFNFIRGSHYPHSQAFL
CCEEECCCEEEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHHCCCCEECCCCCCCHHHHH
DACDREGMCMLNEGIFWGMGGFKEHDRYWNCDAYPAEKKDRIAFEESCMRQVREMVLQFR
HHCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NHPSIVIWSISNEPFFTRHAPEARALCNRLITLVKELDPTRPVCAGGGQRGNFDKLGDMA
CCCCEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHCCEE
ALNGDGSHVKTPGRPSMVTEYGSVSCRRPGAYAPGWGDMKKDKEAGIRYPWRVGEAVWCG
EECCCCCCCCCCCCCCEEECCCCCEECCCCCCCCCCCCCCCHHHCCCCCCEECCCEEEEE
FDHGSIWPSGGRMGIVDYFRIPKRAWYWYRNALRNIPPPEWPVEGTPAQVKLSADKKVIS
CCCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEC
PADGTDDVHVTVKVADAAGRQISNAVPVTLTVVSGPGEFPTGKSITFTPGTDIDLIDGCA
CCCCCCCEEEEEEEECCCCCHHCCCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHCCEE
AIEFRSYYAGKTVIRASSPGLKGDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQ
EEEEHHHHCCCEEEEECCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCEE
LARYGRPESGEKANLAVLRPCSASSGFQETMKASDGDDVSAWHPSVEDKAPWWQLDMEFE
HEECCCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
FRLDRVEVKAAGSWKGPAPSVQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIR
EEEEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEEEE
LSPGQGIAEVAVWPADAS
ECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA