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The map label for this gene is rimO

Identifier: 187736112

GI number: 187736112

Start: 1954359

End: 1955717

Strand: Reverse

Name: rimO

Synonym: Amuc_1623

Alternate gene names: 187736112

Gene position: 1955717-1954359 (Counterclockwise)

Preceding gene: 187736113

Following gene: 187736110

Centisome position: 73.41

GC content: 58.28

Gene sequence:

>1359_bases
ATGCCTCTCACCGTTGGACTCATTTCCCTGGGCTGCCCCAAGAATCTGGTTGACTCTGAAATCATGATCGGCCACCTGCA
AAAAGCCGGCATGACCATGACTCCGGAACCGGAACTCGCGGACGTAATGGTGGTCAATACCTGCGCTTTCATTGACCAGG
CCAAGCAGGAAGCCATCGATGCCATTCTGGACATCGTGCGTGCCCGGGAAAATGGGGCTTATCCGGAAAACCAGAAACTC
ATCGTGGCGGGCTGCCTGTCCCAGCGCTTCCGGAAGGAACTGCCCGCCCTGCTTCCGGAGGTGGACGCCTTTATCGGTCC
GGACCAAATCACCAAACTGCCGGAAATCATCACTCAAGTGATGGACCGGACCATACAGGACCGGAACTTCATTGAAGGAA
AATGCAGGTACGTTCCTGACTGGAACACGCCGCGCTACCGCCTTACCCCGCCGCACACGGCCTACATCAAGATAGCGGAA
GGCTGCAACCACGGCTGCGCCTACTGCATCATTCCCATGATCCGCGGACGCCACCGCAGCCGTTCCCAGCAGGACGTAGT
GCGTGAAGCGGAAACCCTTATCCGGTCCGGCGTGAAGGAAATCTGCCTCATCGCGCAGGACATCACCTATTACGGCATGG
ATAAATGGACGGATGCGCGCCCAAACCGCCGCAGCGCGGTGGATTCCTCCCGCGGAGAATCCCTGGCTTCCCTGCTCCGC
GCTCTGAACGCCATTGAGGGAGAATTCTGGATCAGGCTGCTCTACACCCACCCCGCCCACTGGAGCGACGAACTGACCGC
AGCCATCGCGGAATGCCCCAAAGTGGCCCGCTATGTAGACATTCCCCTACAGCATATTTCCGATAATATGCTGGACGCCA
TGCAGCGCGTAACGGACGGAAACTATATCCGCACGTTGCTCCGGAATATCCGGAAGGCCGTTCCGGGCATCGCCATCCGC
ACCACCTTCATCACCGGATTCCCCGGAGAAACGGAAGACGACCATCAGGAACTGATGGAGTTTATCGAGGAATTCCGTTT
TGAACGCGCCGGCATTTTCACCTTCTCACGGGAGGAAGGGACAAAAGCCTACAAGATGCCCAACCAGGTGCACCACCGCA
CCAAGGCTCGCCGCTACAATGAAGCCACCCTGTTGCTGGCGCGTCTCGCTTCGGAAACGGGCCAGGAACAAATCGGCAGG
CAAATACGCGTGCTGGTGGATGCTCCCGGAGTCGCCAGAACGGAATGGGATGCTCCGGACATTGACGGAACCGTCTCCGT
TCCTCTGACGCTGCCCGTCGGGCAATTCGCCACTGTCACCGTAACGGATGCCGTTGCGTATGAATTGACGGCGGAATAA

Upstream 100 bases:

>100_bases
CCCCTGTGCGCGTCGTGGACATCCGGCCATTCGACCTCGTCGTCGTCAAGGACGACTCCCCCTCCGGGGATTGACGCCAT
CACGCTTCTTATCTACTGCC

Downstream 100 bases:

>100_bases
AATTGCTTCCGTGCCTGTCGGAAAGAACCCGGGAACAAAAATACAGGCGAAGCGCAGGCCGACGTATATTGCCTCCTGTT
TTCCGCCCGGCTTTCCGGAT

Product: MiaB-like tRNA modifying enzyme YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMVVNTCAFIDQAKQEAIDAILDIVRARENGAYPENQKL
IVAGCLSQRFRKELPALLPEVDAFIGPDQITKLPEIITQVMDRTIQDRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAE
GCNHGCAYCIIPMIRGRHRSRSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTDARPNRRSAVDSSRGESLASLLR
ALNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVARYVDIPLQHISDNMLDAMQRVTDGNYIRTLLRNIRKAVPGIAIR
TTFITGFPGETEDDHQELMEFIEEFRFERAGIFTFSREEGTKAYKMPNQVHHRTKARRYNEATLLLARLASETGQEQIGR
QIRVLVDAPGVARTEWDAPDIDGTVSVPLTLPVGQFATVTVTDAVAYELTAE

Sequences:

>Translated_452_residues
MPLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMVVNTCAFIDQAKQEAIDAILDIVRARENGAYPENQKL
IVAGCLSQRFRKELPALLPEVDAFIGPDQITKLPEIITQVMDRTIQDRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAE
GCNHGCAYCIIPMIRGRHRSRSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTDARPNRRSAVDSSRGESLASLLR
ALNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVARYVDIPLQHISDNMLDAMQRVTDGNYIRTLLRNIRKAVPGIAIR
TTFITGFPGETEDDHQELMEFIEEFRFERAGIFTFSREEGTKAYKMPNQVHHRTKARRYNEATLLLARLASETGQEQIGR
QIRVLVDAPGVARTEWDAPDIDGTVSVPLTLPVGQFATVTVTDAVAYELTAE
>Mature_451_residues
PLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMVVNTCAFIDQAKQEAIDAILDIVRARENGAYPENQKLI
VAGCLSQRFRKELPALLPEVDAFIGPDQITKLPEIITQVMDRTIQDRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAEG
CNHGCAYCIIPMIRGRHRSRSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTDARPNRRSAVDSSRGESLASLLRA
LNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVARYVDIPLQHISDNMLDAMQRVTDGNYIRTLLRNIRKAVPGIAIRT
TFITGFPGETEDDHQELMEFIEEFRFERAGIFTFSREEGTKAYKMPNQVHHRTKARRYNEATLLLARLASETGQEQIGRQ
IRVLVDAPGVARTEWDAPDIDGTVSVPLTLPVGQFATVTVTDAVAYELTAE

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=419, Percent_Identity=27.6849642004773, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI28872784, Length=419, Percent_Identity=27.6849642004773, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=29.0953545232274, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=36.4035087719298, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1786882, Length=454, Percent_Identity=29.7356828193833, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=27.4468085106383, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71996771, Length=331, Percent_Identity=29.0030211480363, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356207, Length=462, Percent_Identity=26.8398268398268, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI19922432, Length=378, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_AKKM8 (B2ULZ9)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878224.1
- ProteinModelPortal:   B2ULZ9
- SMR:   B2ULZ9
- GeneID:   6274722
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1623
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK2335883
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50865; Mature: 50734

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMVVNTCAFIDQAKQEAID
CCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AILDIVRARENGAYPENQKLIVAGCLSQRFRKELPALLPEVDAFIGPDQITKLPEIITQV
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MDRTIQDRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAEGCNHGCAYCIIPMIRGRHRS
HHHHHHCCCCCCCCEEECCCCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
RSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTDARPNRRSAVDSSRGESLASLLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHH
ALNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVARYVDIPLQHISDNMLDAMQRVTDG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
NYIRTLLRNIRKAVPGIAIRTTFITGFPGETEDDHQELMEFIEEFRFERAGIFTFSREEG
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCC
TKAYKMPNQVHHRTKARRYNEATLLLARLASETGQEQIGRQIRVLVDAPGVARTEWDAPD
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCCC
IDGTVSVPLTLPVGQFATVTVTDAVAYELTAE
CCCEEEEEEEECCCCEEEEEEEHHEEEEEECC
>Mature Secondary Structure 
PLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMVVNTCAFIDQAKQEAID
CEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AILDIVRARENGAYPENQKLIVAGCLSQRFRKELPALLPEVDAFIGPDQITKLPEIITQV
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MDRTIQDRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAEGCNHGCAYCIIPMIRGRHRS
HHHHHHCCCCCCCCEEECCCCCCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
RSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTDARPNRRSAVDSSRGESLASLLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHH
ALNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVARYVDIPLQHISDNMLDAMQRVTDG
HHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
NYIRTLLRNIRKAVPGIAIRTTFITGFPGETEDDHQELMEFIEEFRFERAGIFTFSREEG
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCC
TKAYKMPNQVHHRTKARRYNEATLLLARLASETGQEQIGRQIRVLVDAPGVARTEWDAPD
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCCC
IDGTVSVPLTLPVGQFATVTVTDAVAYELTAE
CCCEEEEEEEECCCCEEEEEEEHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA