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The map label for this gene is rluE [H]

Identifier: 187736067

GI number: 187736067

Start: 1899649

End: 1900215

Strand: Reverse

Name: rluE [H]

Synonym: Amuc_1578

Alternate gene names: 187736067

Gene position: 1900215-1899649 (Counterclockwise)

Preceding gene: 187736070

Following gene: 187736066

Centisome position: 71.33

GC content: 61.02

Gene sequence:

>567_bases
ATGATTCTGGCGTTCAATAAACCTTACGGTATTCTCTCCCAATTTACGAAGGAGGCTCCCCACCACCGGACACTGGCTGA
ATTCGGGTTTCCTCCTGGCGTGTATCCTGTAGGAAGGCTGGATGCGGATTCGGAAGGGCTGCTTCTTTTATCCGATGAGA
AGGGCCTGGCGGACCGTCTCCTGAACCCCTCCGGCAGGCATCCCAGAACTTATTGGAGCCAGGTGGAAGGCACTCCTTCC
GAAACGGACCTTCGCCCTCTGGAACTCGGGGGCCTCCGCATCCGCGGGCACTGCACGCTGCCTTGCCGGGCACGCCTCAT
GCGCAGGGAGCCTGACGTGCCGCCCCGCATCCCTCCCGTCCGCTACCGCGCCTCCATTCCCACCTCATGGCTGGAATTGA
CGCTGGTGGAAGGGAAAAACCGCCAGGTGCGGCGCATGACCGCCGCCATCGGTTTTCCCACACTCCGGCTTCTTCGCGCC
AGAATCGGAAATTTCTCCCTGGCGGGACTGAAACCCGGCGCATGGAAAGAACTGGATTCCCGCGAGCGAAGGCAGCTCAT
GCCGTAA

Upstream 100 bases:

>100_bases
CATGCATGCAGATACTTTCCTTCCCCAACACAGACAAGTCAAGCCGCTTCAATTTCCCGTGCCAAAGCCAGCGGTTCATG
CTACCCTTGCCGTGCACGGC

Downstream 100 bases:

>100_bases
TATAAGCTTGATTCCGGACAGGAACCGTTTTTATATCTCTCTCCGCATCAGGATATCTACCTAGACCTTTTTACATGAAA
GCCGAACTCGTCGAACAAGC

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MILAFNKPYGILSQFTKEAPHHRTLAEFGFPPGVYPVGRLDADSEGLLLLSDEKGLADRLLNPSGRHPRTYWSQVEGTPS
ETDLRPLELGGLRIRGHCTLPCRARLMRREPDVPPRIPPVRYRASIPTSWLELTLVEGKNRQVRRMTAAIGFPTLRLLRA
RIGNFSLAGLKPGAWKELDSRERRQLMP

Sequences:

>Translated_188_residues
MILAFNKPYGILSQFTKEAPHHRTLAEFGFPPGVYPVGRLDADSEGLLLLSDEKGLADRLLNPSGRHPRTYWSQVEGTPS
ETDLRPLELGGLRIRGHCTLPCRARLMRREPDVPPRIPPVRYRASIPTSWLELTLVEGKNRQVRRMTAAIGFPTLRLLRA
RIGNFSLAGLKPGAWKELDSRERRQLMP
>Mature_188_residues
MILAFNKPYGILSQFTKEAPHHRTLAEFGFPPGVYPVGRLDADSEGLLLLSDEKGLADRLLNPSGRHPRTYWSQVEGTPS
ETDLRPLELGGLRIRGHCTLPCRARLMRREPDVPPRIPPVRYRASIPTSWLELTLVEGKNRQVRRMTAAIGFPTLRLLRA
RIGNFSLAGLKPGAWKELDSRERRQLMP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=50.2824858757062, Blast_Score=164, Evalue=3e-42,
Organism=Escherichia coli, GI1790453, Length=183, Percent_Identity=30.0546448087432, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787524, Length=185, Percent_Identity=33.5135135135135, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 21268; Mature: 21268

Theoretical pI: Translated: 11.01; Mature: 11.01

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAFNKPYGILSQFTKEAPHHRTLAEFGFPPGVYPVGRLDADSEGLLLLSDEKGLADRL
CEEEECCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHH
LNPSGRHPRTYWSQVEGTPSETDLRPLELGGLRIRGHCTLPCRARLMRREPDVPPRIPPV
CCCCCCCCCHHHHHCCCCCCCCCCCEEEECCEEEEEEECCHHHHHHHHCCCCCCCCCCCE
RYRASIPTSWLELTLVEGKNRQVRRMTAAIGFPTLRLLRARIGNFSLAGLKPGAWKELDS
EEECCCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCHHHHH
RERRQLMP
HHHHHCCC
>Mature Secondary Structure
MILAFNKPYGILSQFTKEAPHHRTLAEFGFPPGVYPVGRLDADSEGLLLLSDEKGLADRL
CEEEECCCHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHH
LNPSGRHPRTYWSQVEGTPSETDLRPLELGGLRIRGHCTLPCRARLMRREPDVPPRIPPV
CCCCCCCCCHHHHHCCCCCCCCCCCEEEECCEEEEEEECCHHHHHHHHCCCCCCCCCCCE
RYRASIPTSWLELTLVEGKNRQVRRMTAAIGFPTLRLLRARIGNFSLAGLKPGAWKELDS
EEECCCCCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCCCHHHHH
RERRQLMP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]