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The map label for this gene is pfkA [H]

Identifier: 187735972

GI number: 187735972

Start: 1771023

End: 1772102

Strand: Direct

Name: pfkA [H]

Synonym: Amuc_1481

Alternate gene names: 187735972

Gene position: 1771023-1772102 (Clockwise)

Preceding gene: 187735971

Following gene: 187735978

Centisome position: 66.48

GC content: 57.41

Gene sequence:

>1080_bases
ATGAACATCGGTGTTTTGAATAGCGGGGGTGACTCCCCCGGGTTGAATGCAGTTATTGAAGGTGTTGTGGGCGCGGCTTC
CCGTCGCGGCTGGAACGTTGTGGGTTTTTACGACGGTTTTGAAGGGCTTTTGTCCGAGGAAGGGGACGAACGGTTTGAAT
GGCTGACCCCTGCCGCCTGCCGCGGATTGCGAGCCAAGGGGGGCACCATTCTGGGAACCGTGAACAAAGGAAACTTCGCC
ATCAAAGTAGGCGTGGACCAAAAGGGGGCGATTGAACCCGCGGTGCTGGAAAAAACGAAGGCTACTGTCCGCCGGCTGGG
ACTGGATGCCCTGATCGTTGTGGGCGGAGACGGCTCCCAGTCCACGGCTCTTTTGCTTTCCGAGATAGGGCTCCCCGTAG
TTGGCGTGCCAAAAACCATTGATAATGACCTGGGCGCAACCGATGTCACTTTTGGCTTCTACAGCGCCGTTTCCATTGTT
TCCGAGTCTTTGGACCGTCTGGAAACCACTGCAAACGCGCACCAGCGCATGATGGTGGTGGAAGTGATGGGACGGCATGC
GGGATGGATTGCCTTGGAAGGCGGCATCGCAGGGAGTGCTGATGTTATTCTTCTGCCGGAAATTCCTTTTTCCCTTGAAA
ACGTGGTGGAATGCATCAAGGCGCGCAAGGCTGCCGGGCAGCGGGAAATCCTGGTGGTGGTATCTGAAGGGGCCCGTCTG
GCGGACGAACTGGTATTGCTGGATGAAAAGACCCAGGGGGAAGTTCGCCTGGGTGGCATCGGCAAGGTGATTGCCAAGAA
ACTGGAAGAAGCCACTGGGATTGAAACCCGCTCCTGCGTGCTGGGCCATATCCAGCGCGGCGGTTCTCCCTGTTCCTATG
ACCGGATTCTGGGAACGCGGTTCGGCAGTTATGCCGTGGAACTGGTGGAAAAGCGCCAATTCAGCTGCATGGTTGCTCTC
CGTGGGACGCAGATGACTGCCGTACCCATTGAGGAAGCTGTGAAAACCTTGAAGCTGGTGGATCCCGATTGTCAGCTGGT
GCGCACGGCCCGCGATTTGGGCGTATGCTTCGGGGACTGA

Upstream 100 bases:

>100_bases
AGGACCCCAAGGAATTTGTCCAGCTTCCTGTGAAATCTGCTCCACAAAGGAGATAGGAAGTAGACGGGGAAGGATTTTTC
TGCTATAAGGAACGCCGAAT

Downstream 100 bases:

>100_bases
CGTTCTCCGTCTGCGTCACGGGAAATTGATTGTACCCGATAGGGTTTTGATTGCTTTTCAACCGTATCCTGGAATCAGGA
TACGGTTATTTTTTGCGGAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLLSEEGDERFEWLTPAACRGLRAKGGTILGTVNKGNFA
IKVGVDQKGAIEPAVLEKTKATVRRLGLDALIVVGGDGSQSTALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIV
SESLDRLETTANAHQRMMVVEVMGRHAGWIALEGGIAGSADVILLPEIPFSLENVVECIKARKAAGQREILVVVSEGARL
ADELVLLDEKTQGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTRFGSYAVELVEKRQFSCMVAL
RGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD

Sequences:

>Translated_359_residues
MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLLSEEGDERFEWLTPAACRGLRAKGGTILGTVNKGNFA
IKVGVDQKGAIEPAVLEKTKATVRRLGLDALIVVGGDGSQSTALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIV
SESLDRLETTANAHQRMMVVEVMGRHAGWIALEGGIAGSADVILLPEIPFSLENVVECIKARKAAGQREILVVVSEGARL
ADELVLLDEKTQGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTRFGSYAVELVEKRQFSCMVAL
RGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD
>Mature_359_residues
MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLLSEEGDERFEWLTPAACRGLRAKGGTILGTVNKGNFA
IKVGVDQKGAIEPAVLEKTKATVRRLGLDALIVVGGDGSQSTALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIV
SESLDRLETTANAHQRMMVVEVMGRHAGWIALEGGIAGSADVILLPEIPFSLENVVECIKARKAAGQREILVVVSEGARL
ADELVLLDEKTQGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTRFGSYAVELVEKRQFSCMVAL
RGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=368, Percent_Identity=32.3369565217391, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI266453619, Length=367, Percent_Identity=32.6975476839237, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI266453768, Length=367, Percent_Identity=32.1525885558583, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI266453748, Length=367, Percent_Identity=32.1525885558583, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI4505749, Length=367, Percent_Identity=32.1525885558583, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI11321601, Length=367, Percent_Identity=32.1525885558583, Blast_Score=148, Evalue=6e-36,
Organism=Escherichia coli, GI1790350, Length=337, Percent_Identity=40.0593471810089, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25147584, Length=369, Percent_Identity=30.0813008130081, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558788, Length=336, Percent_Identity=32.1428571428571, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI72003601, Length=324, Percent_Identity=26.5432098765432, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323861, Length=372, Percent_Identity=34.4086021505376, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6321679, Length=371, Percent_Identity=32.6145552560647, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17647809, Length=367, Percent_Identity=31.6076294277929, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652337, Length=367, Percent_Identity=31.6076294277929, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28573326, Length=367, Percent_Identity=31.8801089918256, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37950; Mature: 37950

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLLSEEGDERFEWLTPAAC
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCHHHHCCHHHH
RGLRAKGGTILGTVNKGNFAIKVGVDQKGAIEPAVLEKTKATVRRLGLDALIVVGGDGSQ
CCCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
STALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIVSESLDRLETTANAHQRMMVV
HHHHHHHHCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVMGRHAGWIALEGGIAGSADVILLPEIPFSLENVVECIKARKAAGQREILVVVSEGARL
HHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHH
ADELVLLDEKTQGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTR
HHHEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
FGSYAVELVEKRQFSCMVALRGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD
HHHHHHHHHHHCCCEEEEEECCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLLSEEGDERFEWLTPAAC
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCCHHHHCCHHHH
RGLRAKGGTILGTVNKGNFAIKVGVDQKGAIEPAVLEKTKATVRRLGLDALIVVGGDGSQ
CCCCCCCCEEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
STALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIVSESLDRLETTANAHQRMMVV
HHHHHHHHCCCCEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVMGRHAGWIALEGGIAGSADVILLPEIPFSLENVVECIKARKAAGQREILVVVSEGARL
HHHCCCCCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHH
ADELVLLDEKTQGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTR
HHHEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHH
FGSYAVELVEKRQFSCMVALRGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD
HHHHHHHHHHHCCCEEEEEECCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA