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The map label for this gene is grpE [H]

Identifier: 187735950

GI number: 187735950

Start: 1750208

End: 1750762

Strand: Direct

Name: grpE [H]

Synonym: Amuc_1459

Alternate gene names: 187735950

Gene position: 1750208-1750762 (Clockwise)

Preceding gene: 187735949

Following gene: 187735951

Centisome position: 65.7

GC content: 57.3

Gene sequence:

>555_bases
ATGAGCGACGAAGAGAAAGAGAAAAACGCGGAAGCGGAAGAGCTGGAGGCTCAGGAACAGGTGCAGGATGCTCCTGCCGA
GGAGAAGGTGGCGGAACCTTCCCTGGAAGAGGAATTGCTTAAATGGCGGGATGCCGCCATGCGCACTGCTGCGGAATATG
ATAACTACCGCAAGCGCATGGTGAAGGAAAAGGAGGAATGCGCCAAGTTCGCCAATCAGCGCCTGCTGGAAGAACTTCTA
CCCGTCATTGATAATTTTGAAATGGGCATGGCCGCCGCCAGCGCAGATGCCTCCTCCATGATTTACATCGGCATGAGCAT
GGTGAAGAAGCAGCTGGACGAATTTCTCGCCGGCAATGGCGTGAGCGCCGTGGAGCCTGTGGTGGGAAGCATGTTTGACC
ACGCCACGGAGGAAGCTCTTCAGCGGGAACCCTCCGACCAGCCGGAAGGCACCGTTCTGCGCGTTATCCGCAAGGGGTAT
ATGCTGAAAGACAGGCTTCTGCGCCCGGCGAACGTGGTTGTCGCCCATACTCCGGAACCCGAACCGCAAGTCTGA

Upstream 100 bases:

>100_bases
CGTAGAAGGGTTGCATCAGGCGCGGTCCATTACGCGTCAAACGTCATCCATGTCTTAGCAGGCCGTTGACGCATCCGGTA
AAAGAATTTACATTGCCCGC

Downstream 100 bases:

>100_bases
CCGTTTTTTTAACATTCACGCCACATGGCTAAAAAAGATTATTACGAAATTCTCGGCGTTTCCAAGGACGCTACTGACGA
TGAGATTAAGAAGGCCTATC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSDEEKEKNAEAEELEAQEQVQDAPAEEKVAEPSLEEELLKWRDAAMRTAAEYDNYRKRMVKEKEECAKFANQRLLEELL
PVIDNFEMGMAAASADASSMIYIGMSMVKKQLDEFLAGNGVSAVEPVVGSMFDHATEEALQREPSDQPEGTVLRVIRKGY
MLKDRLLRPANVVVAHTPEPEPQV

Sequences:

>Translated_184_residues
MSDEEKEKNAEAEELEAQEQVQDAPAEEKVAEPSLEEELLKWRDAAMRTAAEYDNYRKRMVKEKEECAKFANQRLLEELL
PVIDNFEMGMAAASADASSMIYIGMSMVKKQLDEFLAGNGVSAVEPVVGSMFDHATEEALQREPSDQPEGTVLRVIRKGY
MLKDRLLRPANVVVAHTPEPEPQV
>Mature_183_residues
SDEEKEKNAEAEELEAQEQVQDAPAEEKVAEPSLEEELLKWRDAAMRTAAEYDNYRKRMVKEKEECAKFANQRLLEELLP
VIDNFEMGMAAASADASSMIYIGMSMVKKQLDEFLAGNGVSAVEPVVGSMFDHATEEALQREPSDQPEGTVLRVIRKGYM
LKDRLLRPANVVVAHTPEPEPQV

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=32.0512820512821, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=30, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324806, Length=180, Percent_Identity=27.2222222222222, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20671; Mature: 20539

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEEKEKNAEAEELEAQEQVQDAPAEEKVAEPSLEEELLKWRDAAMRTAAEYDNYRKRM
CCCCCHHHCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEKEECAKFANQRLLEELLPVIDNFEMGMAAASADASSMIYIGMSMVKKQLDEFLAGNG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSAVEPVVGSMFDHATEEALQREPSDQPEGTVLRVIRKGYMLKDRLLRPANVVVAHTPEP
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EPQV
CCCC
>Mature Secondary Structure 
SDEEKEKNAEAEELEAQEQVQDAPAEEKVAEPSLEEELLKWRDAAMRTAAEYDNYRKRM
CCCCHHHCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VKEKEECAKFANQRLLEELLPVIDNFEMGMAAASADASSMIYIGMSMVKKQLDEFLAGNG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSAVEPVVGSMFDHATEEALQREPSDQPEGTVLRVIRKGYMLKDRLLRPANVVVAHTPEP
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EPQV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA