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The map label for this gene is yfkO [H]

Identifier: 187735931

GI number: 187735931

Start: 1730320

End: 1730967

Strand: Direct

Name: yfkO [H]

Synonym: Amuc_1440

Alternate gene names: 187735931

Gene position: 1730320-1730967 (Clockwise)

Preceding gene: 187735930

Following gene: 187735934

Centisome position: 64.95

GC content: 57.41

Gene sequence:

>648_bases
ATGAATATGAACGCTCAGGAATTGATCGAGACGCTGGAATGGCGTTACGCCACCAAGGTATTCAATCCGGACCGCCGCAT
TCCGGCGGAGGACTGGGAAGCCCTTCTGGAATCCCTGCATTTGAGCCCTTCCTCCCTGGGGCTTCAGATGTGGAAGTTCC
TTGATGTCCGGGATCCCGCCGTGCGTTCCCGGCTTAGGGAAGTTTCATGGGGACAGCCCCAGGTTACGGACGCTTCCCAT
CTGGTGGTTTTCTGTGCCCGCAGGGATTTCAAGCCGGAAGACGTGCAGCGCTATCTGGAACGTATTGTGGAAGTGCGCGG
TGTTGCCATGGACTCCCTGGACCAGTACCGCGCCCGCATTTTTGAACTGGTGGGTTCCAAGTCTCCGGAAGTGCTGAAAG
CCTGGCTGGAACGGCAGGTGTACATTGCCCTCGGGTTCATGATGAGCTGCGCCGCGGATCTCCGCGTGGACACCTGCGCG
CTGGAAGGGATGGATCCGGCCGCCTATGACCGCATTCTTCATCTGGAAAATTCCCCTTATTACACGCTTTGCGCCCTGGC
TCTGGGTTACCGGAATGAAGAGGCGGATAAGTACGCCCGTCTGGCGAAAGTACGTTTTGACCGGGATGAAGTCATTCCGG
TCATCTGA

Upstream 100 bases:

>100_bases
GAACCTTGGCTTGCATTCCGGCAGGGAAACGGGGAAAATGCCAAGGCATGGAGCAAGCTGCGGCGGAAAACCGTCCGGCT
TCCTGCACGAATAATCATAA

Downstream 100 bases:

>100_bases
CGGAGTTTCTTACTAATCCCCGGTTTCAAGCAGTAGCCGCATGTCTTCCGGCAGGGGGGCTTCCGCGGTGAAGGTTTCTT
CATCAAACGGAAATTCCAGC

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPSSLGLQMWKFLDVRDPAVRSRLREVSWGQPQVTDASH
LVVFCARRDFKPEDVQRYLERIVEVRGVAMDSLDQYRARIFELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCA
LEGMDPAAYDRILHLENSPYYTLCALALGYRNEEADKYARLAKVRFDRDEVIPVI

Sequences:

>Translated_215_residues
MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPSSLGLQMWKFLDVRDPAVRSRLREVSWGQPQVTDASH
LVVFCARRDFKPEDVQRYLERIVEVRGVAMDSLDQYRARIFELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCA
LEGMDPAAYDRILHLENSPYYTLCALALGYRNEEADKYARLAKVRFDRDEVIPVI
>Mature_215_residues
MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPSSLGLQMWKFLDVRDPAVRSRLREVSWGQPQVTDASH
LVVFCARRDFKPEDVQRYLERIVEVRGVAMDSLDQYRARIFELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCA
LEGMDPAAYDRILHLENSPYYTLCALALGYRNEEADKYARLAKVRFDRDEVIPVI

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24933; Mature: 24933

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPSSLGLQMWKFLDVRDPA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
VRSRLREVSWGQPQVTDASHLVVFCARRDFKPEDVQRYLERIVEVRGVAMDSLDQYRARI
HHHHHHHCCCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCALEGMDPAAYDRILHLENSPY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
YTLCALALGYRNEEADKYARLAKVRFDRDEVIPVI
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPSSLGLQMWKFLDVRDPA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
VRSRLREVSWGQPQVTDASHLVVFCARRDFKPEDVQRYLERIVEVRGVAMDSLDQYRARI
HHHHHHHCCCCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCALEGMDPAAYDRILHLENSPY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCH
YTLCALALGYRNEEADKYARLAKVRFDRDEVIPVI
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]