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The map label for this gene is hisS [H]

Identifier: 187735890

GI number: 187735890

Start: 1669309

End: 1670583

Strand: Reverse

Name: hisS [H]

Synonym: Amuc_1397

Alternate gene names: 187735890

Gene position: 1670583-1669309 (Counterclockwise)

Preceding gene: 187735896

Following gene: 187735889

Centisome position: 62.71

GC content: 59.06

Gene sequence:

>1275_bases
ATGCCAGACGCTCGCTTTCAACCACTTCCCGGATTCCGCGATTTTGCACCGGCGGATTGCGCCGTGCGCAACTATCTTTT
CAACGCATGGAAGAAGGTGGCGCATCGTTACGGATTCCTGGAATGGGAGGGCCCGACTGTGGAAGCCACGGAGCTTTATC
TTAAAAAGAGCGGAGGCGAGCTGCCCACCCAGCTCTTCCGCTTCACGGACCAGGGGGACCGGGACATCACCATCCGCCCG
GAACTCACCGCCTCCCTGGGACGCATCGCAGCCGCTTATCAGCGCGAATACACCAAACCGCTCAAGTGGTTTGAAATCGG
CTCATGCTTCCGTTATGAAAAACCCCAGAAAGGGCGTCTGCGCGAATTTTATCAATTCAATGCGGATATTCTGGGAGAAG
CCTCCGCGTGGGCGGATTCCGAACTGATAGCCCTGGCCATCGACTGCATGAGGGAACTGGGCTTTACGCAAAACGACTTC
ATTGTGCGCGTCTCTGACCGGGAAGCCTGGATCAGGTTTGCTTCGGAACACGGCGTGCAGGAACAGGATATTCCCGCTTT
CCTCGGCATCGTGGACAAATTTGAACGTGACCGCCCGGAAGAATGCCAGCGCAAGCTGGACGCCTTTCATATCAGCCGCG
GCGATCTGGTGGCCTTCATTGAAAACCCGCCCGCCGGAGCGTCCGAACGCTATGACATCCTGATGAAAGACCTGACGGCC
CGCGGGCTGGACGGCTATGTCAAGCTGGACTTATCCGTAGTGCGCGGACTGGCATACTACACCGGTCTGGTTTTTGAAAT
CTTTGACACACAGCGCAGCCTGCGCGCCGTAGCCGGTGGAGGACGTTACGACACGCTGGTAGGCGCCCTTTCCAACAACG
CAGTGGACATGCCGGCAACCGGTTTTGCCATGGGGGATGCCGTCATCACCCACCTTATTGAACAGACGCCCCATGCCAGG
GCGCTGAAAGATGCGGCGCTGGCCTCCGCAGGCTGCGACATCTTCATGGTTCAGGCATCTGAAAGCCGCCGCGCGGAAGT
TCTTGCCATCGTCTCCGCCCTCCGGGACCAGGGATACAGTGTGGACCTTCCCCTCACGCTCACCAAAGTCAACGGACAGC
TCCAGAAGGCCGTCAAATCCGGCGCCCGCGCGGCCTTGATCGTGGGAGATGAATTCCCGGTCATGGAACTTCGCGACCTG
GGCGCGCGCACCTCATCCCCCGTCGCCATGGACGACCTGTTTGACGCCGTGGCTTCACTCACCGGAAGCAATTGA

Upstream 100 bases:

>100_bases
GTTTCCACCGAAATTCCTTCATCATAAGCATGTGGACTTGCCAAGGCATAGGAATTCTGTAAACTGTTTCGTCCTCAGAC
AACATCCTTTTTTCCAGGTT

Downstream 100 bases:

>100_bases
CACCGTTCTTTCCCCTTCTTCCATTTTCAATAAAGATGAACTCATACCGCACTCATACCTGCAGCGAATTGCGCGCTGCG
AACATCGGCAAGCCGACCAC

Product: Histidine--tRNA ligase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPDARFQPLPGFRDFAPADCAVRNYLFNAWKKVAHRYGFLEWEGPTVEATELYLKKSGGELPTQLFRFTDQGDRDITIRP
ELTASLGRIAAAYQREYTKPLKWFEIGSCFRYEKPQKGRLREFYQFNADILGEASAWADSELIALAIDCMRELGFTQNDF
IVRVSDREAWIRFASEHGVQEQDIPAFLGIVDKFERDRPEECQRKLDAFHISRGDLVAFIENPPAGASERYDILMKDLTA
RGLDGYVKLDLSVVRGLAYYTGLVFEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPATGFAMGDAVITHLIEQTPHAR
ALKDAALASAGCDIFMVQASESRRAEVLAIVSALRDQGYSVDLPLTLTKVNGQLQKAVKSGARAALIVGDEFPVMELRDL
GARTSSPVAMDDLFDAVASLTGSN

Sequences:

>Translated_424_residues
MPDARFQPLPGFRDFAPADCAVRNYLFNAWKKVAHRYGFLEWEGPTVEATELYLKKSGGELPTQLFRFTDQGDRDITIRP
ELTASLGRIAAAYQREYTKPLKWFEIGSCFRYEKPQKGRLREFYQFNADILGEASAWADSELIALAIDCMRELGFTQNDF
IVRVSDREAWIRFASEHGVQEQDIPAFLGIVDKFERDRPEECQRKLDAFHISRGDLVAFIENPPAGASERYDILMKDLTA
RGLDGYVKLDLSVVRGLAYYTGLVFEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPATGFAMGDAVITHLIEQTPHAR
ALKDAALASAGCDIFMVQASESRRAEVLAIVSALRDQGYSVDLPLTLTKVNGQLQKAVKSGARAALIVGDEFPVMELRDL
GARTSSPVAMDDLFDAVASLTGSN
>Mature_423_residues
PDARFQPLPGFRDFAPADCAVRNYLFNAWKKVAHRYGFLEWEGPTVEATELYLKKSGGELPTQLFRFTDQGDRDITIRPE
LTASLGRIAAAYQREYTKPLKWFEIGSCFRYEKPQKGRLREFYQFNADILGEASAWADSELIALAIDCMRELGFTQNDFI
VRVSDREAWIRFASEHGVQEQDIPAFLGIVDKFERDRPEECQRKLDAFHISRGDLVAFIENPPAGASERYDILMKDLTAR
GLDGYVKLDLSVVRGLAYYTGLVFEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPATGFAMGDAVITHLIEQTPHARA
LKDAALASAGCDIFMVQASESRRAEVLAIVSALRDQGYSVDLPLTLTKVNGQLQKAVKSGARAALIVGDEFPVMELRDLG
ARTSSPVAMDDLFDAVASLTGSN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=453, Percent_Identity=26.0485651214128, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI15029520, Length=457, Percent_Identity=24.945295404814, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1788861, Length=448, Percent_Identity=27.9017857142857, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71993693, Length=461, Percent_Identity=22.9934924078091, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=461, Percent_Identity=22.9934924078091, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=443, Percent_Identity=24.6049661399549, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24643061, Length=457, Percent_Identity=25.164113785558, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24643059, Length=475, Percent_Identity=24.2105263157895, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47049; Mature: 46918

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDARFQPLPGFRDFAPADCAVRNYLFNAWKKVAHRYGFLEWEGPTVEATELYLKKSGGE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCC
LPTQLFRFTDQGDRDITIRPELTASLGRIAAAYQREYTKPLKWFEIGSCFRYEKPQKGRL
CHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHCCCCCCCCHH
REFYQFNADILGEASAWADSELIALAIDCMRELGFTQNDFIVRVSDREAWIRFASEHGVQ
HHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCC
EQDIPAFLGIVDKFERDRPEECQRKLDAFHISRGDLVAFIENPPAGASERYDILMKDLTA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
RGLDGYVKLDLSVVRGLAYYTGLVFEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPAT
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
GFAMGDAVITHLIEQTPHARALKDAALASAGCDIFMVQASESRRAEVLAIVSALRDQGYS
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCE
VDLPLTLTKVNGQLQKAVKSGARAALIVGDEFPVMELRDLGARTSSPVAMDDLFDAVASL
ECCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
TGSN
CCCC
>Mature Secondary Structure 
PDARFQPLPGFRDFAPADCAVRNYLFNAWKKVAHRYGFLEWEGPTVEATELYLKKSGGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHEECCCCC
LPTQLFRFTDQGDRDITIRPELTASLGRIAAAYQREYTKPLKWFEIGSCFRYEKPQKGRL
CHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHCCCCCCCCHH
REFYQFNADILGEASAWADSELIALAIDCMRELGFTQNDFIVRVSDREAWIRFASEHGVQ
HHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCC
EQDIPAFLGIVDKFERDRPEECQRKLDAFHISRGDLVAFIENPPAGASERYDILMKDLTA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHHHH
RGLDGYVKLDLSVVRGLAYYTGLVFEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPAT
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
GFAMGDAVITHLIEQTPHARALKDAALASAGCDIFMVQASESRRAEVLAIVSALRDQGYS
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCE
VDLPLTLTKVNGQLQKAVKSGARAALIVGDEFPVMELRDLGARTSSPVAMDDLFDAVASL
ECCCEEEEECCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
TGSN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA