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The map label for this gene is purN [H]

Identifier: 187735775

GI number: 187735775

Start: 1543459

End: 1544046

Strand: Direct

Name: purN [H]

Synonym: Amuc_1281

Alternate gene names: 187735775

Gene position: 1543459-1544046 (Clockwise)

Preceding gene: 187735765

Following gene: 187735776

Centisome position: 57.94

GC content: 59.35

Gene sequence:

>588_bases
ATGTCCAGATTGCCCAAGTTAGGAATACTCGGTTCCGGTTCCGGTTCCAATTGCCAGTCCATTTATGATGCCATCCAGTC
CGGTTCTCTCCGGGCGGAAATCGCCGTGGTGATGTCTGACAATCCGGACGCCTATATTCTGGAACGCGCCCGTTCCTGGG
GCATTCCGGCGGAGGTTATTGACTGCGGGGGATTTAAAACCAGATTTCCGGAGGAATCCCAGGCATCCGTTGCCGCGCGC
CTGAAGCAATACGGCGTGGACTGCGTGTGCCTGGCGGGATTCATGCGCTTGGTGAAACTTCCGCTGCTGAAGGAATTTCC
TTCCCGCATCCTGAATATCCACCCTTCCCTTCTTCCAGCCTTCCCGGGGCTTCATGCCTGGGAGCAGGCTGTGAACGCCG
GAGCTGCGGAGAGCGGCTGCACCGTCCATTATGTGGATGACGGAATGGATACCGGCCCCATCCTGGGCCAGGCTCGCGTG
CCCGTGCTGCCGGGGGATACGCCGGAAAGCCTTCACGCCCGCATTCAGGAGCAGGAGCATACCCTTTATCCCGCCATGAT
CGCCCGCGTTCTGGAAACGCTGGCATGA

Upstream 100 bases:

>100_bases
GAAAACGGGGGTCTCTCATGCCTTTATTAGGCAAATACGGCAGCCTTTTTGTCAAGCCGGAGACTTGCCACGGGCAAAAA
ACTGTGGTTTTATCCCCCGG

Downstream 100 bases:

>100_bases
CCTGATTCTCCTTCTGACCGGAAAGCAATCCGGCCTCCTGCCGGGAGAGGATGACAGGATGACCGATTCTCTTGTCTTGT
GATATGAACGGAATCCGTTA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDAYILERARSWGIPAEVIDCGGFKTRFPEESQASVAAR
LKQYGVDCVCLAGFMRLVKLPLLKEFPSRILNIHPSLLPAFPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARV
PVLPGDTPESLHARIQEQEHTLYPAMIARVLETLA

Sequences:

>Translated_195_residues
MSRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDAYILERARSWGIPAEVIDCGGFKTRFPEESQASVAAR
LKQYGVDCVCLAGFMRLVKLPLLKEFPSRILNIHPSLLPAFPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARV
PVLPGDTPESLHARIQEQEHTLYPAMIARVLETLA
>Mature_194_residues
SRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDAYILERARSWGIPAEVIDCGGFKTRFPEESQASVAARL
KQYGVDCVCLAGFMRLVKLPLLKEFPSRILNIHPSLLPAFPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARVP
VLPGDTPESLHARIQEQEHTLYPAMIARVLETLA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=38.9189189189189, Blast_Score=144, Evalue=4e-35,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=38.9189189189189, Blast_Score=144, Evalue=4e-35,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=38.9189189189189, Blast_Score=144, Evalue=4e-35,
Organism=Escherichia coli, GI1788846, Length=180, Percent_Identity=41.6666666666667, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1787483, Length=166, Percent_Identity=34.3373493975904, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=191, Percent_Identity=39.7905759162304, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=212, Percent_Identity=30.6603773584906, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=43.5897435897436, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20983; Mature: 20852

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDAYILERARSWGIPAEVI
CCCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCHHHE
DCGGFKTRFPEESQASVAARLKQYGVDCVCLAGFMRLVKLPLLKEFPSRILNIHPSLLPA
ECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
FPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARVPVLPGDTPESLHARIQEQEH
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLYPAMIARVLETLA
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
SRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDAYILERARSWGIPAEVI
CCCCCCCEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCHHHE
DCGGFKTRFPEESQASVAARLKQYGVDCVCLAGFMRLVKLPLLKEFPSRILNIHPSLLPA
ECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
FPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARVPVLPGDTPESLHARIQEQEH
CCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TLYPAMIARVLETLA
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]