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The map label for this gene is fcl [H]

Identifier: 187735742

GI number: 187735742

Start: 1498142

End: 1499230

Strand: Reverse

Name: fcl [H]

Synonym: Amuc_1248

Alternate gene names: 187735742

Gene position: 1499230-1498142 (Counterclockwise)

Preceding gene: 187735743

Following gene: 187735741

Centisome position: 56.28

GC content: 58.03

Gene sequence:

>1089_bases
ATGAAAAAAAACAGCAGAATCTTTGTGGCCGGACACCACGGCCTCGTCGGTTCCGCCATCTGGAAATCCCTGGAAGACAA
AGGCTATTCCCACCTGATTGGCCGGCCGCACAAAGAACTGGATCTGGAAGACCCTGCGGCCGTCCGGGAGTTTTTTGACC
GGGAAAAGCCGGAATACGTCTTTCTGGCAGCGGCGTTTGTGGGCGGCATCATCGCCAACTCCCGCTACCGGGCGGACTTC
ATCTTCCGCAACCTTCAGATTCAGCAGAACGTCATCGGGGAAAGCTTCCGGCATGGCGTATCCAAGCTCCTCTTCCTGGG
GAGCACCTGCATCTACCCGCGCGAAGCTCCCCAGCCCATGAAGGAAAACGCCCTGCTCACCTCCCCGCTGGAATACACCA
ACGAACCCTATGCCATTGCCAAAATAGCAGGGCTGAAAATGTGCGAAAGCTTCAACCTGCAATACGGCACCAACTACATC
GCCGTCATGCCTACAAATCTGTACGGCCCCAATGACAATTTCCACCTGGAAAACAGCCACGTGCTGCCCGCCATGGTACG
CAAGATACACCTGGCCAAGTGCCTGATGGAGGGAGACTGGAACGCCGTGCGGAAAGATCTGAACGCGCGCCCAGTGGAAC
AGGTGGACGGCACGGCTGAAGAAAAGGAAATCCTGGCCGTGCTGGCCAAATACGGCATTACCCCCGGTTCCGTGGAACTG
TGGGGTACGGGAACTCCCCTGCGAGAGTTCCTGTGGAGCGAAGACATGGCGGACGCCTGCGTCCATGTGATGGAGCAAGT
GGATTTCAGCCAGCTGCAAGAAGGAGGAAGGGAAGTGCGGAACTGCCACATCAATATCGGAACCGGGAAAGAAATCTCCA
TCGGCGGACTGGCCCGCCTGATTGCCGCTACGGCAGGGTACCGGGGAAATCTGGCGTTCAACGCAGACAAGCCGGACGGC
ACCATGCGCAAGCTGACGGACGTCTCCAAGCTGCACTCCCTGGGCTGGAAACACCGGGTGGAGCTGGAAGAGGGAGTGGA
ACGCATTTACCGGTGGTATCTGAGCCGGATGGAAGCCGACGGCAACTGA

Upstream 100 bases:

>100_bases
CTTCTTGGCTGGAACCCCACCTCCACTCCGTTTGAGGAACTCGTGCGCATCATGGTGCGCCACGACATGAAATACGTAAA
AACTCCCGTTCATTTCTGAG

Downstream 100 bases:

>100_bases
CTTTTCGGCCAGCTCCCCTCACTCCAATGACCAGCCCCCCGGACAACCAGGATACGCCCCCCGCCAAAGCCAGCCTGTGG
GCGCTGAGCCCCCTGCTGGT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKKNSRIFVAGHHGLVGSAIWKSLEDKGYSHLIGRPHKELDLEDPAAVREFFDREKPEYVFLAAAFVGGIIANSRYRADF
IFRNLQIQQNVIGESFRHGVSKLLFLGSTCIYPREAPQPMKENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYI
AVMPTNLYGPNDNFHLENSHVLPAMVRKIHLAKCLMEGDWNAVRKDLNARPVEQVDGTAEEKEILAVLAKYGITPGSVEL
WGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGREVRNCHINIGTGKEISIGGLARLIAATAGYRGNLAFNADKPDG
TMRKLTDVSKLHSLGWKHRVELEEGVERIYRWYLSRMEADGN

Sequences:

>Translated_362_residues
MKKNSRIFVAGHHGLVGSAIWKSLEDKGYSHLIGRPHKELDLEDPAAVREFFDREKPEYVFLAAAFVGGIIANSRYRADF
IFRNLQIQQNVIGESFRHGVSKLLFLGSTCIYPREAPQPMKENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYI
AVMPTNLYGPNDNFHLENSHVLPAMVRKIHLAKCLMEGDWNAVRKDLNARPVEQVDGTAEEKEILAVLAKYGITPGSVEL
WGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGREVRNCHINIGTGKEISIGGLARLIAATAGYRGNLAFNADKPDG
TMRKLTDVSKLHSLGWKHRVELEEGVERIYRWYLSRMEADGN
>Mature_362_residues
MKKNSRIFVAGHHGLVGSAIWKSLEDKGYSHLIGRPHKELDLEDPAAVREFFDREKPEYVFLAAAFVGGIIANSRYRADF
IFRNLQIQQNVIGESFRHGVSKLLFLGSTCIYPREAPQPMKENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYI
AVMPTNLYGPNDNFHLENSHVLPAMVRKIHLAKCLMEGDWNAVRKDLNARPVEQVDGTAEEKEILAVLAKYGITPGSVEL
WGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGREVRNCHINIGTGKEISIGGLARLIAATAGYRGNLAFNADKPDG
TMRKLTDVSKLHSLGWKHRVELEEGVERIYRWYLSRMEADGN

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=362, Percent_Identity=29.2817679558011, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1788365, Length=353, Percent_Identity=47.3087818696884, Blast_Score=327, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17554428, Length=359, Percent_Identity=27.0194986072423, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922778, Length=359, Percent_Identity=27.0194986072423, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 40655; Mature: 40655

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNSRIFVAGHHGLVGSAIWKSLEDKGYSHLIGRPHKELDLEDPAAVREFFDREKPEYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
FLAAAFVGGIIANSRYRADFIFRNLQIQQNVIGESFRHGVSKLLFLGSTCIYPREAPQPM
EEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
KENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYIAVMPTNLYGPNDNFHLENSH
HCCCEEECCHHHCCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCEEECCCC
VLPAMVRKIHLAKCLMEGDWNAVRKDLNARPVEQVDGTAEEKEILAVLAKYGITPGSVEL
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEE
WGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGREVRNCHINIGTGKEISIGGLARL
EECCCHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCHHHHEEEEEECCCCEECHHHHHHH
IAATAGYRGNLAFNADKPDGTMRKLTDVSKLHSLGWKHRVELEEGVERIYRWYLSRMEAD
HHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCC
GN
CC
>Mature Secondary Structure
MKKNSRIFVAGHHGLVGSAIWKSLEDKGYSHLIGRPHKELDLEDPAAVREFFDREKPEYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
FLAAAFVGGIIANSRYRADFIFRNLQIQQNVIGESFRHGVSKLLFLGSTCIYPREAPQPM
EEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
KENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYIAVMPTNLYGPNDNFHLENSH
HCCCEEECCHHHCCCCCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCEEECCCC
VLPAMVRKIHLAKCLMEGDWNAVRKDLNARPVEQVDGTAEEKEILAVLAKYGITPGSVEL
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCEEE
WGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGREVRNCHINIGTGKEISIGGLARL
EECCCHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCHHHHEEEEEECCCCEECHHHHHHH
IAATAGYRGNLAFNADKPDGTMRKLTDVSKLHSLGWKHRVELEEGVERIYRWYLSRMEAD
HHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHCCC
GN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]